Immunoglobulin molecular genetics: the prospects for mutational analysis of the chromosomal immunoglobulin genes
Keyword(s):
Homologous recombination between transferred and chromosomal DNA can be used to effect precise, predetermined modifications of the chromosomal genes. Ultimately this phenomenon should allow the assessment of genetic regulatory elements as they function in the normal chromosomal environment. We have previously described a system for isolating mutant hybridoma cells that are defective in immunoglobulin (Ig) production, with a view toward using these mutants to define cis-acting elements that influence Ig gene expression. Here we describe results that indicate that homologous recombination between transferred and chromosomal Ig genes can be used to map Ig mutations by marker rescue.Key words: homologous recombination, gene expression.
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1992 ◽
Vol 12
(3)
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pp. 1202-1208
Keyword(s):
1993 ◽
Vol 13
(9)
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pp. 5928-5928
1990 ◽
Vol 10
(9)
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pp. 4466-4472
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2018 ◽
2002 ◽
Vol 227
(5)
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pp. 301-314
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Keyword(s):
1990 ◽
Vol 10
(9)
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pp. 4466-4472
1988 ◽
Vol 8
(10)
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pp. 4041-4047
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