Dielectrophoretic separation of living cells

1971 ◽  
Vol 17 (7) ◽  
pp. 879-888 ◽  
Author(s):  
B. D. Mason ◽  
P. M. Townsley
Keyword(s):  
Complex Function ◽  
Pulse Height ◽  
Cell Types ◽  
Living Cells ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
Pulse Height Analyzer ◽  
Physiological Character ◽  
Cells Cultured

Dielectrophoretic separation studies were undertaken to investigate the possibility of separating cells with varying physiological character. An attempt was made to define the importance of size in the determination of separability. Living cells of Saccharomyces cerevisiae var. ellipsoideus were separated from the same cells killed by heating, using a batch separation. Cells cultured in wort broth were separated from cells grown in yeast nitrogen base – 1% glucose using both batch and continuous separators. Size analyses of separated fractions were performed using a Coulter transducer/multichannel pulse height analyzer. A size differential in cell separability was demonstrated, although it is clear that a complex function of both size and polarizability is instrumental in determining the separation of cell types.

scholarly journals DETERMINATION OF THE URANIUM CONTENT IN SOME SOIL SAMPLES FROM AJAOKUTA, KOGI STATE: NIGERIA

2019 ◽  
Vol 7 (9) ◽  
pp. 252-258
Author(s):  
Aruwa Arome ◽  
Philibus Musa Gyuk ◽  
Achor Mathias Ogwo ◽  
Isah Suleiman
Keyword(s):  
Activity Concentration ◽  
Pulse Height ◽  
Uranium Content ◽  
Mean Value ◽  
Soil Samples ◽  
Pulse Height Analyzer ◽  
The World ◽  
The Mean ◽  
Good Agreement

This work present the Uranium (238U) content in soil samples collected in Ajaokuta from some villages was been determined. The measurement of the soil Uranium activity concentration were made using a multi–channel pulse height analyzer (Camberra series 10 plus) coupled to a 76.2mm x 76.2mm NaI (TI) scintillation detector. 2.93)The mean Uranium content in the analyzed samples was found to be (44.26 Bq/kg which is higher than the world mean value of 35Bq/kg. The results were in good agreement with others for soils from region which is considered as normal or slightly high in radioactivity level.

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

par-4, a gene required for cytoplasmic localization and determination of specific cell types in Caenorhabditis elegans embryogenesis.

Genetics ◽  
1992 ◽  
Vol 130 (4) ◽  
pp. 771-790 ◽  
Author(s):  
D G Morton ◽  
J M Roos ◽  
K J Kemphues
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Types ◽  
Intestinal Cells ◽  
Specific Cell ◽  
Cytoplasmic Localization ◽  
Loss Of Function ◽  
Embryo Viability ◽  
Cell Stage ◽  
Maternal Genome

Abstract Specification of some cell fates in the early Caenorhabditis elegans embryo is mediated by cytoplasmic localization under control of the maternal genome. Using nine newly isolated mutations, and two existing mutations, we have analyzed the role of the maternally expressed gene par-4 in cytoplasmic localization. We recovered seven new par-4 alleles in screens for maternal effect lethal mutations that result in failure to differentiate intestinal cells. Two additional par-4 mutations were identified in noncomplementation screens using strains with a high frequency of transposon mobility. All 11 mutations cause defects early in development of embryos produced by homozygous mutant mothers. Analysis with a deficiency in the region indicates that it33 is a strong loss-of-function mutation. par-4(it33) terminal stage embryos contain many cells, but show no morphogenesis, and are lacking intestinal cells. Temperature shifts with the it57ts allele suggest that the critical period for both intestinal differentiation and embryo viability begins during oogenesis, about 1.5 hr before fertilization, and ends before the four-cell stage. We propose that the primary function of the par-4 gene is to act as part of a maternally encoded system for cytoplasmic localization in the first cell cycle, with par-4 playing a particularly important role in the determination of intestine. Analysis of a par-4; par-2 double mutant suggests that par-4 and par-2 gene products interact in this system.

scholarly journals Multiplexed Plasmonic Nano-Labeling for Bioimaging of Cytological Stained Samples

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3509
Author(s):  
Paule Marcoux-Valiquette ◽  
Cécile Darviot ◽  
Lu Wang ◽  
Andrée-Anne Grosset ◽  
Morteza Hasanzadeh Kafshgari ◽  
...  
Keyword(s):  
Accurate Determination ◽  
Cell Types ◽  
Fine Needle Biopsy ◽  
Dark Field ◽  
Imaging Method ◽  
New Methods ◽  
Formalin Fixed Paraffin ◽  
Dark Field Microscopy ◽  
Formalin Fixed Paraffin Embedded

Reliable cytopathological diagnosis requires new methods and approaches for the rapid and accurate determination of all cell types. This is especially important when the number of cells is limited, such as in the cytological samples of fine-needle biopsy. Immunoplasmonic-multiplexed- labeling may be one of the emerging solutions to such problems. However, to be accepted and used by the practicing pathologists, new methods must be compatible and complementary with existing cytopathology approaches where counterstaining is central to the correct interpretation of immunolabeling. In addition, the optical detection and imaging setup for immunoplasmonic-multiplexed-labeling must be implemented on the same cytopathological microscope, not interfere with standard H&E imaging, and operate as a second easy-to-use imaging method. In this article, we present multiplex imaging of four types of nanoplasmonic markers on two types of H&E-stained cytological specimens (formalin-fixed paraffin embedded and non-embedded adherent cancer cells) using a specially designed adapter for SI dark-field microscopy. The obtained results confirm the effectiveness of the proposed optical method for quantitative and multiplex identification of various plasmonic NPs, and the possibility of using immunoplasmonic-multiplexed-labeling for cytopathological diagnostics.

Toxicity of Glycols and Allyl Alcohol Evaluated by Means of Co-cultures of Microcarrier-attached Rat Hepatocytes and BALB/C 3T3 Mouse Fibroblasts

1992 ◽  
Vol 20 (2) ◽  
pp. 266-270
Author(s):  
Jens-Uwe Voss ◽  
Hasso Seibert
Keyword(s):  
Lactate Dehydrogenase ◽  
Ethylene Glycol ◽  
Allyl Alcohol ◽  
Rat Hepatocytes ◽  
3T3 Cells ◽  
Active Metabolites ◽  
Mediated Effects ◽  
Cells Cultured

The toxicity of allyl alcohol and several glycols (ethylene glycol, 1,2-propanediol, 1,3-propanediol, methoxyethanol, and the glycol ether dioxane) was studied in cultures of 3T3 cells and in co-cultures of 3T3 cells with microcarrier-attached hepatocytes. Metabolism-mediated effects on the cytotoxicity to 3T3 cells were recorded by differences in the growth of the cultures exposed in the presence or absence of hepatocytes. Hepatocyte viability was determined by depletion of intracellular lactate dehydrogenase and effects on the biotransformation ability of hepatocytes were assessed by determination of O-deethylation of 7-ethoxycoumarin (EOD activity). Allyl alcohol was the only substance more toxic to the hepatocytes than to 3T3 cells cultured in the absence of hepatocytes. Toxicity to 3T3 cells of allyl alcohol, ethylene glycol, and 1,3-propanediol, but not of 1,2-propanediol, methoxyethanol and dioxane, was markedly enhanced when the cells were co-cultured with hepatocytes. The results indicate that the toxicity of allyl alcohol, ethylene glycol, and 1,3-propanediol, to 3T3 cells depends on the formation of active metabolites. For ethylene glycol and 1,3-propanediol, growth of 3T3 cells in co-cultures was reduced at concentrations without effects on hepatocyte viability. Co-culture of 3T3 cells with microcarrier-attached rat hepatocytes represents a suitable approach for the in vitro evaluation of metabolism-mediated cytotoxicity.

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