scholarly journals CHARACTERIZATION OF INULIN FROM BLACK SALSIFY (SCORZONERA HISPANICA L.) FOR FOOD AND PHARMACEUTICAL PURPOSES

2018 ◽  
Vol 11 (12) ◽  
pp. 221 ◽  
Author(s):  
Nadezhda Petkova
Keyword(s):  
High Performance ◽  
Chemical Characterization ◽  
Degree Of Polymerization ◽  
Size Exclusion ◽  
Future Application ◽  
Coating Agent ◽  
Swelling Capacity ◽  
Exclusion Chromatography ◽  
Water Holding

Objective: The aim of the current study was to evaluate the black salsify (Scorzonera hispanica L.) as a potential source of inulin and to characterize the physicochemical properties of isolated polysaccharide.Methods: The carbohydrate content in its roots and leaves was analyzed by high-performance liquid chromatography with refractive index detection (HPLC-RID) method. Microwave-assisted extraction was performed for isolation of inulin from black salsify roots. The obtained polysaccharide was characterized by HPLC-RID method, HPLC size-exclusion chromatography, and Fourier transformed-infrared spectroscopy. Functional properties as swelling capacity, solubility, and water-holding and oil-holding capacities (OHCs) were also evaluated.Results: Black salsify (S. hispanica L.) roots were evaluated as a rich source of inulin (22% dw) and 1-kestose (6.25 g/100 g dw). The isolated inulin (yield 20%) was characterized with average degree of polymerization 17, with polydispersity index (1.04) that was near to medium-chained inulin. This polysaccharide showed better OHC than water-holding capacity, and it was characterized with swelling capacity 0.5 ml/g sample.Conclusion: For the first time, inulin was isolated from black salsify roots. The chemical characterization of inulin reveals the potential of this plant to be used as a valuable source of this polysaccharide for future application in food technology and pharmaceutical industry for dietary fibers, stabilizer, and coating agent.

Chemical characterization of polymerized products formed in the reactions of matairesinol and pinoresinol with the stable radical 2,2-diphenyl-1-picrylhydrazyl

Holzforschung ◽  
2012 ◽  
Vol 66 (3) ◽  
Author(s):  
Annika I. Smeds ◽  
Patrik C. Eklund ◽  
Evanthia Monogioudi ◽  
Stefan M. Willför
Keyword(s):  
High Performance ◽  
Chemical Characterization ◽  
Stable Radical ◽  
Size Exclusion ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Exclusion Chromatography ◽  
Tof Ms

Abstract Polymerization reactions of the lignans matairesinol and pinoresinol with the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) were studied. The reactions were rapid and almost quantitative. The molecular weight distribution (MWD) of the formed lignan dimers and polymers was determined by high-performance size-exclusion chromatography (HPSEC) combined with evaporative light-scattering detection (ELSD), and the compounds were characterized by HPLC-MS, pyrolysis-GC/MS, NMR, MALDI-ToF-MS, and GC-MS of the monomeric and dimeric units formed by KMnO4 oxidation. The yield of high-MW polymers is higher for pinoresinol (69%) than for matairesinol (43%). According to the HPSEC-ELSD analyses, the MWD is also broader for the pinoresinol polymers. The latter seem to consist mainly of hepta-hexacontalignans (MW 2.5–21.4 kDa), whereas the matairesinol polymers are mainly penta-pentadecalignans (MW 1.8–5.3 kDa). The polymers seem to contain mainly unmodified lignan units linked by 5-5′ bonds.

The degradation of Indulin ATR by Streptomyces: chemical characterization of the water soluble acid precipitable products

1989 ◽  
Vol 67 (2) ◽  
pp. 350-355 ◽  
Author(s):  
P. F. Vidal ◽  
J. Bouchard ◽  
E. Chornet ◽  
R. P. Overend ◽  
H. Giroux ◽  
...  
Keyword(s):  
High Performance ◽  
Protein Complexes ◽  
Chemical Characterization ◽  
Water Soluble ◽  
Size Exclusion ◽  
Produce Water ◽  
Lignin Fraction ◽  
Exclusion Chromatography ◽  
Soluble Acid

Two strains of Streptomyces, S. badius and S. virdosporus, when incubated with kraft lignin (Indulin ATR) produce water soluble acid precipitable compounds. We show by a combination of elemental analysis, amino acid analysis, and FTIR that these are complexes of lignin fragments strongly associated with proteins. The latter, which can be separated by solvent extraction, are added by the microorganisms themselves. The lignin fraction, which represents approximately 60% of the complex weight, consists of lower molecular weight, lower methoxyl content Indulin fragments. KeyWords: lignin, Indulin, lignin–protein complexes, Fourier transform infrared spectrophotometry, high performance size exclusion chromatography, Streptomyces.

scholarly journals Characterization of fractionated lignins polymerized by fungal laccases

BioResources ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. 1105-1121 ◽  
Author(s):  
Daniel van de Pas ◽  
Aynsley Hickson ◽  
Lloyd Donaldson ◽  
Gareth Lloyd-Jones ◽  
Tarja Tamminen ◽  
...  
Keyword(s):  
Value Added ◽  
Degree Of Polymerization ◽  
Size Exclusion ◽  
Enzyme Treatment ◽  
Chemical Fractionation ◽  
Trametes Hirsuta ◽  
Melanocarpus Albomyces ◽  
Exclusion Chromatography ◽  
Optimum Reaction

Lignins are important biopolymers that can be converted into value-added materials by enzymatic treatments. However, the heterogeneity of the lignin polymer makes it a challenging material to modify. Thus, chemical fractionation was used to obtain lignins with high homogeneity in order to assess their biotechnological utilization. Commercial Alcell, birch organosolv lignins, and steam-exploded pine and eucalypt lignins were sequentially fractionated by ether, ether/acetone 4:1 (v:v), and acetone. All fractions were structurally characterized prior to treatments with Thielavia arenaria, Trametes hirsuta, and Melanocarpus albomyces laccases. The reactivities of the enzymes towards the lignins were determined by oxygen consumption measurements, and the degree of polymerization was confirmed by size exclusion chromatography. Field emission scanning electron microscopy revealed that the surfaces of the lignin nanoparticles were dispersed in the enzyme treatment, suggesting an increase in hydrophilicity of the surfaces detected as loosened morphology. Hence, it was concluded that enzyme-aided valorization is an attractive means for lignin modification, provided that optimum reaction conditions are employed.

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