ISOLATION OF RAT HEPARIN

1959 ◽  
Vol 37 (1) ◽  
pp. 1183-1186
Author(s):  
L. B. Jaques
Keyword(s):  
Body Weight ◽  
Sodium Salt ◽  
Anticoagulant Activity ◽  
Barium Salt ◽  
Wistar Strain

Heparin was isolated from the whole carcass and skin of rats of the Wistar strain and crystallized as the barium salt. The sodium salt derived from this had a specific anticoagulant activity (Howell assay) of 47.5 International Units/mg and specific metachromatic activity of 101.5 International Units/mg. Yield of heparin was 0.55 mg/100 g body weight.

ISOLATION OF RAT HEPARIN

1959 ◽  
Vol 37 (10) ◽  
pp. 1183-1186 ◽  
Author(s):  
L. B. Jaques
Keyword(s):  
Body Weight ◽  
Sodium Salt ◽  
Anticoagulant Activity ◽  
Barium Salt ◽  
Wistar Strain

Heparin was isolated from the whole carcass and skin of rats of the Wistar strain and crystallized as the barium salt. The sodium salt derived from this had a specific anticoagulant activity (Howell assay) of 47.5 International Units/mg and specific metachromatic activity of 101.5 International Units/mg. Yield of heparin was 0.55 mg/100 g body weight.

scholarly journals Eine neue Methode zur Herstellung und Reinigung von 5-Methyltetrahydrofolsäure / A New Method for Preparation and Purification of 5-M ethyltetrahydrofolic Acid

1973 ◽  
Vol 28 (11-12) ◽  
pp. 650-652
Author(s):  
Harold Rüdiger ◽  
Reinhard Siede
Keyword(s):  
Acetic Acid ◽  
Folic Acid ◽  
Ion Exchange ◽  
Sodium Borohydride ◽  
Sodium Salt ◽  
Barium Salt ◽  
Borohydride Reduction ◽  
Methyl Derivative ◽  
Crude Product ◽  
Sodium Borohydride Reduction

Abstract 5-Methyltetrahydrofolic acid is prepared from folic acid by sodium borohydride reduction, reac­ tion with formaldehyde and reduction to the methyl derivative by sodium borohydride. The crude product is precipitated as barium salt which after having been converted to the sodium salt by ion exchange is adsorbed to QAE-Sephadex and eluted by different acetic acid gradients in subsequent chromatographies. This method allows to process gram quantities on reasonably small columns

scholarly journals Efficacy of Composite Extract from Leaves and Fruits of Medicinal Plants Used in Traditional Diabetic Therapy against Oxidative Stress in Alloxan-Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Brahm Kumar Tiwari ◽  
Dileep Kumar ◽  
A. B. Abidi ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Diabetic Complications ◽  
Small Dose ◽  
Diabetic Rats ◽  
Vital Role ◽  
Special Focus ◽  
Wistar Strain ◽  
Glucose Plasma

Oxidative stress plays a vital role in diabetic complications. To suppress the oxidative stress mediated damage in diabetic pathophysiology, a special focus has been given on composite extract (CE) and making small dose of naturally occurring antidiabetic plants leaf and fruits. The aim of the present study was to evaluate the beneficial role of CE against alloxan- (ALX-) induced diabetes of Wistar strain rats. A dose-dependent study for CE (25, 50, and 100 mg/kg body weight) was carried out to find the effective dose of the composite compound in ALX-induced diabetic rats. ALX exposure elevated the blood glucose, plasma advanced oxidation product (AOPP), sialic acid demonstrating disturbed antioxidant status.CE at a dose of 100 mg/kg body weight restored/minimised these alterations towards normal values. In conclusion, small dose of CE possesses the capability of ameliorating the oxidative stress in ALX-induced diabetes and thus could be a promising approach in lessening diabetic complications.

scholarly journals Pengaruh Kontrasepsi Suntik DMPA Terhadap Kenaikan Berat Badan Tikus Betina Galur Wistar

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwi Retna Prihati
Keyword(s):  
Body Weight ◽  
Test Group ◽  
Laboratory Research ◽  
Control Group ◽  
Female Rat ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Laboratory ◽  
Rat Strain

Abstract: DMPA Injectable Contraception, Body Weight. The purpose of this study to determine the effect of contraception DMPA injection to BB in female blood wistar strain female. The benefits of this study are expected to increase public knowledge about injectable contraception especially related to the side effects of weight gain. This type of research is an experimental laboratory. Research design Randomized pre-post test group with control, the sample size of 10 adult female mice Wistar strain divided into 2 groups. Data were analyzed by independent T test. Significant value of p <0.05. The result of this research is there is significant difference between control group and DMPA contraception treatment group (p = 0,008). In conclusion contraceptive DMPA affects body weight wistar female rat strain.

scholarly journals The effects of crude extract of Amaranthus hybridus (000059604) on reproductive parameters of the male Wistar rats

2020 ◽  
Vol 47 (4) ◽  
pp. 44-57
Author(s):  
M. O. Oyeyemi ◽  
V. B. Adedeji ◽  
A. M. Bankole
Keyword(s):  
Body Weight ◽  
Antioxidant Properties ◽  
Albino Rats ◽  
Reproductive Parameters ◽  
Amaranthus Hybridus ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Male Wistar Rats ◽  
Group A ◽  
Group B

Studies on Amaranthus hybridus (AH) have established its antioxidant properties. This study investigated the effects of AH on some reproductive parameters of forty-five sexually matured Albino rats (Wistar Strain) males. Amaranthus hybridus has been reported to have several protective and curative properties attributed majorly to strong antioxidant activity. They were randomly grouped into 3 with each group consisting of 15 rats. Group A received (per Os) Amaranthus hybridus (50mg/kg body weight) and group B had Amaranthus hybridus (100mg/kg body weight for 7 days. Group C (control) received distilled water for 7 days. Blood and semen samples were collected for analyses on day 1,8,15 and 22 post-treatments. The results showed that the sperm motility of group B in day 1, (2.00± 2.00%) rat was significantly lower (p < 0.001) than that of group C (26.00 ± 7.97%), whereas it was greatly improved in Group B (85.00 ± 4.47%) and Group A (60.00 ±4.47%) at the day 22 posttreatment. Livability of sperm cells were significantly higher (p < 0.5 0) in Group A (96.20±0.73%) and Group B (96.20±0.73%) compared to Group C (96.80±1.64%) at day 22 post-treatment. The histology of the testis of control and all treatment groups revealed normal testicular germinal epithelium full of viable spermatogenic cells. It is, therefore, concluded that Amaranthus hybridus extract given orally at 50mg/kg and 100mg/kg body weight, did not affect the normal cytoarchitecture of the testis and epididymis and can increase sperm production.

scholarly journals EFFECT OF ETHANOLIC EXTRACT OF TINOSPORA CRISPA L FROM INDONESIA IN ALLOXAN INDUCED LIVER DAMAGE

2017 ◽  
Vol 9 (8) ◽  
pp. 65
Author(s):  
Emsutrisna . ◽  
Fahrizal Aria Sahadewa ◽  
Ikbar Ardiansyah
Keyword(s):  
Body Weight ◽  
Liver Damage ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Hepatoprotective Effect ◽  
Male Rats ◽  
Histopathologic Examination ◽  
Wistar Strain ◽  
Alloxan Monohydrate ◽  
Group 1

Objective: The objective of this study was to evaluate the hepatoprotective effect of Tinospora crispa L. (Bratawali).Methods: Twenty four male rats wistar strain were divided into four groups. The serum alanine amino transferase (ALT) and aspartate aminotransferase (AST) of rats were measured (day 0). Rats then were injected by alloxan monohydrate at doses of 120 mg/200 bw (g) intraperitoneally. Four days later, the serum ALT and AST of rats were measured (second measurement/day 4) and then were treated by extract appropriate their groups. Group 1 was treated by 2 ml of distilled water orally; group 2,3 and 4 were treated by 70% ethanolic extract of T. crispa L. (EETC) at dose of 100; 200 and 400 mg/200 bw (g)/day respectively orally. After 10 d treatment, serum ALT and AST were measured (third measurement/day 14). At the end of this treatment, all rats were killed for histopathologic examination of their liver. The histopathologic examination was performed to assess the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei.Results: The result of this study showed that the ethanolic extract of T. crispa L at dose of 100 and 200 mg/200gbw can reduce blood ALT and AST significantly (P<0.05). From the histopathological examination, it was found that the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei of EETC at doses of 100 and 200 mg/200 body weight (g) lower than negative control.Conclusion: The present study shows that the 70% EETC at dose of 100 and 200 mg/200 body weight (g) has hepatoprotective effect against alloxan induced liver damage.  

ISOLATION AND IDENTIFICATION OF THE FAST-DEATH FACTOR IN MICROCYSTIS AERUGINOSA NRC-1

1959 ◽  
Vol 37 (1) ◽  
pp. 453-471 ◽  
Author(s):  
C. T. Bishop ◽  
E. F. L. J. Anet ◽  
P. R. Gorham
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Body Weight ◽  
Microcystis Aeruginosa ◽  
Sodium Salt ◽  
Cyclic Peptide ◽  
Isolation And Identification ◽  
Toxic Compound ◽  
Molar Ratios ◽  
Crude Preparation

The fast-death factor in Microcystis aeruginosa NRC-1 is an acidic, probably cyclic peptide containing the following amino acids in the molar ratios indicated: L-aspartic (1); L-glutamic (2); D-serine (1); L-valine (1); L-ornithine (1); L-alanine (2); L-leucine (2). It is possible, although not likely, that one of the residues of glutamic, alanine, or leucine also is in the D-configuration. The toxin, in the form of its sodium salt, was extracted from lyophilized algal cells by water, separated from pigments by extraction into n-butanol, and freed from high-molecular-weight impurities by dialysis. No separation of a single toxic compound could be obtained by countercurrent distribution, chromatography, or electrophoresis in carbonate, acetate, or phosphate buffers. Electrophoresis of the crude toxin on cellulose in 0.1 M borate yielded five peptides one of which was toxic and accounted for 100% of the toxicity present in the crude preparation. The intraperitoneal LD50of the pure toxin for mice was 0.466 ± 0.013 mg/kg body weight.

scholarly journals EVALUATION OF THE ACUTE TOXICITY OF LIANA BARK OF LANDOLPHIA OWARIENSIS P. BEAUV. (APOCYNACEAE)

2020 ◽  
Vol 8 (4) ◽  
pp. 167-172
Author(s):  
Dally Laba Ismael ◽  
Fofana Yaya ◽  
Kablan Ahmont Landry Claude ◽  
Lia Arthur José
Keyword(s):  
Nervous System ◽  
Body Weight ◽  
Acute Toxicity ◽  
Wistar Strain ◽  
Sensory Nervous System ◽  
Oral Doses

The objective of this study was to assess the acute toxicity of the decocted bark of the liana bark of L. owriensis P. Beauv. (Apocynaceae) in the Wistar strain rat. The study of acute toxicity was carried out according to the OECD 423 method. This study was carried out up to a limit dose of 5000 mg / kg of body weight. After administration of different oral doses, observations on behavior, respiration, skin effects, response of the sensory nervous system or the gastrointestinal effects of animals were made on the 1st, 2nd, 3rd and 14 days (OECD 423). During 14 days of observation, a decoction of the bark of liana from L. owriensis did not show any mortality or lead to obvious toxicity on the mice at the respective doses of 300, 2000 and 5000 mg / kg. The decoction is not toxic to rats, even in large doses (5000 mg / kg of body weight). Therefore, the vine barks of this plant could be used for effective therapeutic purposes.

scholarly journals Research of subchronic inhalation toxicity of ozone-air mixture

2020 ◽  
pp. 40-43
Author(s):  
Valery Anatolyevich Belyaev ◽  
Vladimir Sergeyevich Nikulin ◽  
Ruslan Rashidovich Kochkarov ◽  
Nikolay Alekseyevich Gvozdetskii ◽  
Tatyana Anatolyevna Beloborodenko
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Blood Parameters ◽  
Behavioral Activity ◽  
Inhalation Toxicity ◽  
Ozone Generation ◽  
Wistar Strain

            The subchronic inhalation toxicity of the ozone-air mixture obtained on a portable ozone generation device (ozonizer) of its own design was studied.  The work investigated the concentration of ozone-air mixture of 1000, 3000 and 5000 ppm.  The experiment was carried out, according to the standards for determining subchronic inhalation toxicity of substances, for 90 days, on rats of the Wistar strain.  Throughout the experiment, body weight, feed intake were recorded, behavioral activity was evaluated, hematological and biochemical blood parameters were determined.  As a result of the study of subchronic inhalation toxicity of the ozone-air mixture, no signs of intoxication were detected in rats, there was no case.

ISOLATION AND IDENTIFICATION OF THE FAST-DEATH FACTOR IN MICROCYSTIS AERUGINOSA NRC-1

1959 ◽  
Vol 37 (3) ◽  
pp. 453-471 ◽  
Author(s):  
C. T. Bishop ◽  
E. F. L. J. Anet ◽  
P. R. Gorham
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Body Weight ◽  
Microcystis Aeruginosa ◽  
Sodium Salt ◽  
Cyclic Peptide ◽  
Isolation And Identification ◽  
Toxic Compound ◽  
Molar Ratios ◽  
Crude Preparation

The fast-death factor in Microcystis aeruginosa NRC-1 is an acidic, probably cyclic peptide containing the following amino acids in the molar ratios indicated: L-aspartic (1); L-glutamic (2); D-serine (1); L-valine (1); L-ornithine (1); L-alanine (2); L-leucine (2). It is possible, although not likely, that one of the residues of glutamic, alanine, or leucine also is in the D-configuration. The toxin, in the form of its sodium salt, was extracted from lyophilized algal cells by water, separated from pigments by extraction into n-butanol, and freed from high-molecular-weight impurities by dialysis. No separation of a single toxic compound could be obtained by countercurrent distribution, chromatography, or electrophoresis in carbonate, acetate, or phosphate buffers. Electrophoresis of the crude toxin on cellulose in 0.1 M borate yielded five peptides one of which was toxic and accounted for 100% of the toxicity present in the crude preparation. The intraperitoneal LD50of the pure toxin for mice was 0.466 ± 0.013 mg/kg body weight.

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