Dyslipidemia’s influence on the secretion ovarian’s steroids in female mice

Introduction: The synthesis ovarian’s steroids is a process thats depends on the supply of cholesterol. Objective: to evaluate the influence of dyslipidemia on the secretion ovarian’s steroids. Methodology: wild female mice were used (C57BL6) and LDL (LDLR-/-), which they were separated into 4 groups (n = 10): WTS: fed a standard diet; WTHL: fed a high-fat diet; KOS: fed a standard diet; KOHL: fed a high-fat diet. After 60 days, the estrous cycle was analyzed and after anesthetized, blood was collected for the to assess the lipid profile, glucose, plasma insulin level and HOMA index was calculated. In addition, plasma levels of C-reactive protein, estrogen and progesterone were determined. Results: The hyperlipidic diet in both the WTHL and the KOHL group generated hypercholesterolemia when compared to the WTS and KOS, respectively, with a decrease in HDLc, associated with an increase in CRP levels. Severe hypercholesterolemia in the KOHL group generated insulin resistance, marked by an increase in HOMAir. Food hypercholesterolemia in the WTHL group, food and genetics in the KOHL group, compared to their WTS and KOS controls, was definitive in reducing plasma levels of estrogen and progesterone. The genetic hypercholesterolemia associated with insulin resistance observed in the KOS and KOHL groups reduced the levels of progesterone, this reduction being more severe in the KOHL group, which had the highest HOMAir. Conclusion: dyslipidemia affected ovarian steroidogenesis in mice by means of oxidative stress, inflammation and insulin resistance and / or by decreasing HDL cholesterol levels.

The Evaluation of Fasting Glucose Plasma (FGP) in Hypertensive Patients using Calcium Channel Blocker (CCB) Drug Class in Undata Regional Hospital

Background: Hypertension plays a major role in the development of heart disease which is one of the most leading causes of death worldwide. Antihypertensives must be selected carefully to avoid affecting metabolic parameters. A risky case involving the increase of glucose plasma levels using Calcium Channel Blocker (CCB) antihypertensive, amlodipine, has been reported. Objectives: This study aims to examine the effects of CCB treatments on blood glucose levels in hypertensive patients at the Undata District General Hospital of Central Sulawesi Province. Material and Methods: This study used a cross-sectional design with a prospective approach. The instruments & materials used in this study are Accu-Chek Performa glucometer along with the patient's blood sample material. The research subjects were 31 patients who met the inclusion criteria of 1) patients who had been treated with CCB therapy for 3 months, 2) were not diagnosed with diabetes mellitus, and 3) did not use drugs that might affect blood glucose levels. Results: The results showed an average value of fasting glucose plasma (FGP) levels of hypertension patients using CCB class showed an increase from baseline 124,619 mg/dL to 141,612 mg/dL with a difference of 16,993 mg/dL. Conclusions: Statistical analysis conducted also obtained a significance value of p 0,000 which indicates that the use of amlodipine can significantly increase fasting patients' glucose plasma levels. If observed clinically, this increase in fasting glucose plasma values ​​falls into the category of mild to moderate hyperglycemic.

Case Report on Schizophrenia

Introduction: The psychotic state of Schizophrenia is characterized by a disruption in the existence of clear consciousness in the thought, emotion and faculties that typically leads to a social abrogation. In India, research found a prevalence 3/1000 individuals. It is more common in men, and when men begin to develop schizophrenia, they appear to be around five years younger by average than women. Case Presentation: A male patient 30 years from Hariom Nagar, Sindhi Meghe, Wardha was admitted to Psychiatric Ward, AVBRH on 20th January 2021 with a case of Schizophrenia. Symptoms: Behavioral changes like muttering to self, smiling to self, aggressiveness, irrelevant talk, sleep disturbance, hearing of voices not heard by others. Non adherence to medication in the last 1 years. Investigation: Hb% - 14.6 mg/dl; Glucose plasma random – 68 mg%; Monocytes- 3%, Granulocytes- 80% Lymphocytes- 15%; HIV, HCV, HBsAg, VDRL, all of these tests were negative. Surgical Management: Patient have no past surgical history. Medical Management: Patient was treated with Tab. Risperidone Plus x HS and Tab Clonazepam 0.25mg. Nursing Management: Assess for physical, psychological and social data. Aware for Impact of schizophrenia on physical health, emotions, thinking and natural capacity to cope. Remember that the defense mechanisms used, the nature or structure of the feeling, the suicidal risk, the capacity to work and the space accessible for social support services. Conclusion: Patient was admitted to AVBRH and was diagnosed to have Schizophrenia. The patient got appropriate treatment and therapy and his condition has improved well.

The Effect of the Decoction of Citrus aurantifolia (Lime) and Camellia sinensis (Lipton Tea) on the Plasma Glucose; Plasma Total Protein, Albumin Concentrations and Weight of Normal Albino Rats

Citrus aurantifolia is one of the major fruit trees that grow throughout the world and it’s known for its refreshing juice and health benefits. Some attributes of citrus aurantifolia (lime) include pharmacological properties (antitumor anti-inflammatory, anti-cancer). This study was aimed at determining the effects of the decoction of citrus aurantifolia and camellia sinensis (Lipton tea) on the glucose, plasma total protein and plasma albumin concentration in normal albino rats. Twenty-four (24) female healthy albino rats of Wister strain aged 3weeks and with an average of weight of 150g were randomly placed into two [2] groups of twelve rats each (control and test) after acclimatization for one week. The lime and Lipton tea was administered orally to the test group for 14days. Blood samples were collected from each rats and analyzed spectrophotometrically for plasma glucose, total protein and albumin. The liver, heart and kidney tissues were collected also for histological studies. Analysis of result shows that the plasma glucose decreased significantly (p<0.05) and the histological report showed that the liver and the kidneys were affected adversely but had no effect on the heart; the plasma total protein decreased significantly (p<0.05) and had adverse affection on the liver and kidneys. There was no significant increase in plasma albumin [p>0.05], however histological report showed effect on the liver and kidneys. In conclusion the lime and Lipton tea can be clinically significant in the reduction of high plasma glucose levels in the management of diabetes mellitus, treatment of obesity due to its protein reducing ability and has effect on plasma albumin; however, concentrations and doses administered could have adverse effect on the liver and kidneys. Key words: Lime, Lipton tea, Glucose, Total protein, Albumin, Albino rats.

Kishk Sa′eedi as a potential functional food for management of metabolic syndrome: A study of the possible interaction with pomegranate seed oil and/ or gum Arabic

Introduction: In the present research, the health benefits of the traditional Egyptian food called Kishk Sa′eedi (KS) and KS mixed with gum Arabic (GA) or with a mixture of GA and pomegranate seed oil (PSO) were studied in a rat model of metabolic syndrome (MS) induced by feeding high fructose high hydrogenated fat diet (HFFD). Methods: Rats were divided into a normal control group (NC) fed on a balanced diet (Diet 1), a MS control (MSC) receiving HFFD (Diet 2), and three test groups feeding on HFFD containing KS (Diet 3), KS with GA (Diet 4), and KS with GA and PSO (Diet 5), respectively for five weeks. Biochemical and histopathological changes were assessed. Results: Significant increase in blood glucose, plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), urea, creatinine, uric acid, malondialdehyde (MDA), dyslipidemia and reduction in reduced glutathione (GSH) were demonstrated in MSC compared to NC (P < 0.05). Significant elevation in liver fat, MDA and gene expression of interleukin-6 (IL-6) with significant down-regulation of peroxisome proliferator-activated receptor (PPAR-α) were noticed in MSC compared to NC (P < 0.05). The three test diets improved plasma high-density lipoprotein-cholesterol (HDL-C), uric acid, MDA, liver PPAR-α and IL-6 expression (P < 0.05) compared to MSC without affecting liver lipids. Blood glucose, plasma dyslipidemia, AST, creatinine and urea were improved by diet 3 and diet 5 (P < 0.05). Diet 3 elevated GSH and reduced ALT and MDA (P < 0.05). Histopathological changes induced by HFFD in both liver and kidney showed variable improvement by feeding the tested diets. Conclusion: The tested diets significantly improved MS rat model with superiority to diet 3.

A case of peritoneal-pericardial leak in a 19-year-old patient on peritoneal dialysis

We report a case of a peritoneal-pericardial leak in peritoneal dialysis.A 19-year-old patient, with no history of heart disease, with unkown chronic kidney disease, treated with continuous ambulatory peritoneal dialysis (CAPD) for 10 months. complained of chest pain and tachycardia, revealing pericardial effusion of great abundance. Pericardial drainage was necessary. The fluid analysis was a transudate with glucose levels 5 times higher than glucose plasma levels. A peritoneal scintigraphy was performed and showed a distribution of the radio-tracer in the peritoneal cavity without any image of a leak. With clinical and especillay biological arguments, the patient was diagnosed with a peritoneal-pericardial leak.After pericardial drainage and temporary switch to hemodialysis, automated peritoneal dialysis was resumed with progressive increase in volumes, without recurrence of the leak after a 6 months follow-up.

Effect of surgical pleth index-guided analgesia versus conventional analgesia techniques on fentanyl consumption under multimodal analgesia in laparoscopic cholecystectomy: a prospective, randomized and controlled study

Background The Surgical Pleth Index (SPI) is an objective tool that can reflect nociception-antinociception balance and guide the use of intraoperative analgesics. Multimodal analgesia has been neglected in many previous studies. The aim of this study was to compare fentanyl consumption using SPI-guided analgesia versus conventional analgesia techniques under multimodal analgesia in laparoscopic cholecystectomy. Methods A total of 80 patients aged 18–65 years with American Society of Anaesthesiologists (ASA) grade I-II and a body mass index (BMI) of 18.5 to 30 kg/m2 who were scheduled for laparoscopic cholecystectomy under total intravenous anaesthesia from March 2020 to September 2020 were selected. Multimodal analgesia, including local infiltration of the surgical incision, nonsteroidal anti-inflammatory drugs and opioids, was adopted perioperatively. Fentanyl boluses of 1.0 µg/kg were administered to maintain the SPI value between 20 and 50 in the SPI group. By contrast, fentanyl boluses of 1.0 µg/kg were administered whenever the heart rate (HR) or mean arterial pressure (MAP) increased to 20 % above baseline or when the HR was greater than 90 beats per minute (bpm) in the control group. Preoperative and postoperative blood glucose, plasma cortisol and interleukin-6 (IL-6) levels were evaluated. Intraoperative haemodynamic events and propofol and fentanyl doses were noted. The extubation time, postoperative visual analogue scale (VAS) score, use of remedial analgesics and opioid-related adverse reactions were recorded. Results In total, 18 of 80 patients withdrew for various reasons, and data from 62 patients were finally analysed. Intraoperative fentanyl consumption was significantly lower in the SPI group than in the control group (177.1 ± 65.9 vs. 213.5 ± 47.5, P = 0.016). The postoperative extubation time was shorter in the SPI group than in the control group (16.1 ± 5.2 vs. 22.1 ± 6.3, P < 0.001). Preoperative and postoperative blood glucose, plasma cortisol and IL-6 levels, intraoperative haemodynamic changes, postoperative VAS scores, remedial analgesic consumption and opioid-related adverse reactions were comparable in the two groups. Conclusions Lower doses of fentanyl are required intraoperatively with shorter extubation times when SPI is used to guide intraoperative analgesia compared to conventional analgesia techniques under multimodal analgesia in laparoscopic cholecystectomy. Trial registration Chictr.org.cn ChiCTR2000030145. Retrospectively Registered (Date of registration: February 24, 2020).

Cytokines Activating ILCs2 in Patients With Obesity Before and After Bariatric Surgery Shortened Title: ILCs2 in Patients With Obesity

Abstract/Purpose: Group 2 innate lymphoid cells (ILC2) are integral regulators of adipose tissue type 2 immunity and respond to epithelial signals such as IL-25, IL-33, thymic stromal lymphopoeitin (TSLP). Purpose was to assess cytokines activating ILCs2 in serum of patients with obesity and effect of bariatric surgery on these parametersMaterial and Methods: In a single-center prospective study serum IL-25, IL-33, TSLP and ST2L levels were assayed at baseline and at 6 months after bariatric surgery and correlated with anthropometric changes and metabolism parameters.Results Mean age and median of BMI of study participants were 41.9 years ± 11 and 45.6 kg/m2 (range 36.3-56.3), respectively. Six months after surgery %EWL was 43.1 ± 10.2%. Serum TSLP was significantly lower in patients with obesity both before and after surgery than in healthy controls. TSLP values before operation were significantly correlated to HbA1c%, glucose plasma level and female sex. Serum IL-25, IL-33 and ST2L levels were comparable to controls both before and after operation. Conclusions: Decreased serum levels of TSLP may be a characteristic trait for obesity, however non-modifiable by body mass surgical reduction in short time observation. Low serum levels of TSLP are related to disturbances in glucose metabolism and female sex.

Therapeutic effects of myocardin-related transcription factor A (MRTF-A) knockout on experimental mice with nonalcoholic steatohepatitis induced by high-fat diet

Objective: To explore the effects of myocardin-related transcription factor A (MRTF-A) knockout on mice with nonalcoholic steatohepatitis (NASH) induced by high-fat diet (HFD). Methods: Normal-fat diet (NFD) or HFD was fed to MRTF-A-knockout (MRTF-A−/−) and wild-type (WT) mice for 16 weeks. Liver histopathological status was observed using Hematoxylin and Eosin (HE) staining, Oil Red O staining, Sirius Red staining, and Immunohistochemical staining. The mRNA and protein levels in liver tissues were measured through quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot. Results: Compared with WT + HFD group, mice in MRTF-A−/− + HFD group were decreased in body weight, blood glucose, plasma insulin, liver TG and NAFLD activity score (NAS), with liver function recovery. Besides, compared with HFD-fed WT mice, HFD-fed MRTF-A−/− mice were improved in hepatic fibrosis, accompanied by decreased collagen content (%) and down-regulated expressions of α-SMA, COL1A2, TGFβ1, and SMAD3. In mice fed with HFD, the expression of MCP-1, CCR2, F4/80 and CD68 declined in liver tissues of MRTF-A−/− mice as compared with WT mice. Besides, in hepatic macrophages isolated from HFD-fed mice, the observed increased expression of TNF-α, IL-1β, MCP-1, as well as decreased expression of CCR2. Compared with WT + HFD group, MRTF-A−/− + HFD group mice were decreased regarding NF-κB p65 in liver tissues. Conclusion: MRTF-A knockout reduced macrophage infiltration, down-regulated NF-κB p65 expression, and ameliorated inflammation and fibrosis of liver tissues in mice, thereby becoming a potential therapeutic target for NASH treatment.

Identification of macrophage activation-related biomarkers in obese type 2 diabetes that may be indicative of enhanced respiratory risk in COVID-19

Hyperactivation of the immune system through obesity and diabetes may enhance infection severity complicated by Acute Respiratory Distress Syndrome (ARDS). The objective was to determine the circulatory biomarkers for macrophage activation at baseline and after serum glucose normalization in obese type 2 diabetes (OT2D) subjects. A case-controlled interventional pilot study in OT2D (n = 23) and control subjects (n = 23). OT2D subjects underwent hyperinsulinemic clamp to normalize serum glucose. Plasma macrophage-related proteins were determined using Slow Off-rate Modified Aptamer-scan plasma protein measurement at baseline (control and OT2D subjects) and after 1-h of insulin clamp (OT2D subjects only). Basal M1 macrophage activation was characterized by elevated levels of M1 macrophage-specific surface proteins, CD80 and CD38, and cytokines or chemokines (CXCL1, CXCL5, RANTES) released by activated M1 macrophages. Two potent M1 macrophage activation markers, CXCL9 and CXCL10, were decreased in OT2D. Activated M2 macrophages were characterized by elevated levels of plasma CD163, TFGβ-1, MMP7 and MMP9 in OT2D. Conventional mediators of both M1 and M2 macrophage activation markers (IFN-γ, IL-4, IL-13) were not altered. No changes were observed in plasma levels of M1/M2 macrophage activation markers in OT2D in response to acute normalization of glycemia. In the basal state, macrophage activation markers are elevated, and these reflect the expression of circulatory cytokines, chemokines, growth factors and matrix metalloproteinases in obese individuals with type 2 diabetes, that were not changed by glucose normalisation. These differences could potentially predispose diabetic individuals to increased infection severity complicated by ARDS. Clinical trial reg. no: NCT03102801; registration date April 6, 2017.