IRRADIATION OF DIPHOSPHOPYRIDINE NUCLEOTIDE IN DILUTE AQUEOUS SOLUTIONS

1960 ◽  
Vol 38 (1) ◽  
pp. 1255-1263 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Absorption Spectrum ◽  
Aqueous Solutions ◽  
Ultraviolet Absorption ◽  
Ultraviolet Absorption Spectrum ◽  
Ph Values ◽  
In Vivo Experiments ◽  
X Irradiation ◽  
Dilute Aqueous Solutions

In extension of previous in vivo experiments, the effects of X irradiation on DPN were studied in vitro. No correlation between the effects on the ultraviolet absorption spectrum and on the coenzyme function of DPN was evident after irradiation at different pH values. However, the loss of coenzyme function could be correlated with the destruction of ribose and of riboside linkages. Catalase did not provide greater protection than did other proteins. It was concluded that the observed loss of DPN from irradiated cells is not due to the radiosensitivity of the DPN molecule itself.

IRRADIATION OF DIPHOSPHOPYRIDINE NUCLEOTIDE IN DILUTE AQUEOUS SOLUTIONS

1960 ◽  
Vol 38 (11) ◽  
pp. 1255-1263 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Absorption Spectrum ◽  
Aqueous Solutions ◽  
Ultraviolet Absorption ◽  
Ultraviolet Absorption Spectrum ◽  
Ph Values ◽  
In Vivo Experiments ◽  
X Irradiation ◽  
Dilute Aqueous Solutions

In extension of previous in vivo experiments, the effects of X irradiation on DPN were studied in vitro. No correlation between the effects on the ultraviolet absorption spectrum and on the coenzyme function of DPN was evident after irradiation at different pH values. However, the loss of coenzyme function could be correlated with the destruction of ribose and of riboside linkages. Catalase did not provide greater protection than did other proteins. It was concluded that the observed loss of DPN from irradiated cells is not due to the radiosensitivity of the DPN molecule itself.

Ultraviolet absorption spectra of the 2-Phenylethyl radical formed during pulse radiolysis

1981 ◽  
Vol 34 (4) ◽  
pp. 721 ◽  
Author(s):  
NA McAskill ◽  
DF Sangster
Keyword(s):  
Absorption Spectrum ◽  
Aqueous Solutions ◽  
Extinction Coefficient ◽  
Pulse Radiolysis ◽  
Electron Attachment ◽  
Ultraviolet Absorption ◽  
Ultraviolet Absorption Spectrum ◽  
Radical Ions ◽  
Dissociation Reaction ◽  
Decarboxylation Reaction

2-Phenylethyl radicals (Ph-CH2-�CH2) were prepared by the decarboxylation reaction of 3-phenylpropanoic acid with sulfate radical ions (SO4-�) formed during the pulse radiolysis of aqueous solutions containing peroxydisulfate (S2O82-) ions. The ultraviolet absorption spectrum of the transient radicals consisted of a band centred at 309nm with an extinction coefficient of 5000 1, mol-1 cm-1. Attempts were made to form phenylethyl radicals by the electron-attachment dissociation reaction of (2-haloethy1)benzenes. Contrary to literature reports, it was found that the latter reaction does not occur in either cyclohexane, benzene or aqueous solutions of (2-chloroethyl)- and (2-bromoethyl)-benzenes.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES

2019 ◽  
Vol 65 (5) ◽  
pp. 760-765
Author(s):  
Margarita Tyndyk ◽  
Irina Popovich ◽  
A. Malek ◽  
R. Samsonov ◽  
N. Germanov ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Human Tumor ◽  
Significant Inhibition ◽  
In Vivo Studies ◽  
Ehrlich Carcinoma ◽  
In Vivo Experiments

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.

scholarly journals Plant-Derived Extracellular Vesicles: Current Findings,Challenges, and Future Applications

Membranes ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 411
Author(s):  
Nader Kameli ◽  
Anya Dragojlovic-Kerkache ◽  
Paul Savelkoul ◽  
Frank R. Stassen
Keyword(s):  
Human Health ◽  
Extracellular Vesicles ◽  
Preliminary Results ◽  
In Vivo Experiments ◽  
Health And Disease ◽  
Conducting Research ◽  
Protocol Standardization ◽  
Insight Into

In recent years, plant-derived extracellular vesicles (PDEVs) have gained the interest of many experts in fields such as microbiology and immunology, and research in this field has exponentially increased. These nano-sized particles have provided researchers with a number of interesting findings, making their application in human health and disease very promising. Both in vitro and in vivo experiments have shown that PDEVs can exhibit a multitude of effects, suggesting that these vesicles may have many potential future applications, including therapeutics and nano-delivery of compounds. While the preliminary results are promising, there are still some challenges to face, such as a lack of protocol standardization, as well as knowledge gaps that need to be filled. This review aims to discuss various aspects of PDEV knowledge, including their preliminary findings, challenges, and future uses, giving insight into the complexity of conducting research in this field.

scholarly journals Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice

2020 ◽  
Vol 22 (1) ◽  
pp. 233
Author(s):  
Eunkuk Park ◽  
Chang Gun Lee ◽  
Eunguk Lim ◽  
Seokjin Hwang ◽  
Seung Hee Yun ◽  
...  
Keyword(s):  
Osteoclast Differentiation ◽  
Osteoblastic Differentiation ◽  
Common Disease ◽  
Root Extract ◽  
Mouse Bone Marrow ◽  
Mineral Density ◽  
Bone Mineral Density Loss ◽  
In Vivo Experiments

Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting alkaline phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis.

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