Chemical and biochemical aspects of superoxide radicals and related species of activated oxygen

1982 ◽  
Vol 60 (11) ◽  
pp. 1330-1345 ◽  
Author(s):  
Ajit Singh

The spectrum of biological processes in which oxygen is used by living systems is quite large, and the products include some damaging species of activated oxygen, particularly the superoxide radical [Formula: see text] and hydrogen peroxide (H2O2). Superoxide radicals and hydrogen peroxide, in turn, can lead to the formation of other damaging species: hydroxyl radicals (∙OH) and singlet oxygen (1O2). Hydroxyl radicals react with organic compounds to give secondary free radicals that, in the presence of oxygen, yield peroxy radicals, peroxides, and hydroperoxides. Formation, interconversion, and reactivity of [Formula: see text] and related activated oxygen species, methods available for their detection, and the basis of their biological toxicity are briefly reviewed.

2009 ◽  
Vol 6 (s1) ◽  
pp. S79-S86 ◽  
Author(s):  
Vikesh Kumar ◽  
M. R. Tripathi ◽  
Manish Kumar ◽  
Gopal Shukla ◽  
Sarvesh Dwivedi ◽  
...  

There are several low lying singlet oxygen (1O2) and superoxide radical (O-2) which are important in photochemical oxidation. In our present analysis we are studying chemical property of singlet oxygen (1O2) and super oxide radical (O-2) and some dyestuffs species to produce reactive oxygen such as singlet oxygen1O2and superoxide radicals. Irradiation with sun lightin vitrothe dyestuff like benzanthrone, metanil yellow andp-aminodiphenylamine were found to produce reactive oxygen species such as singlet oxygen (1O2) and/or superoxide radicals (O-2) .The dose response relationship between singlet1O2production when sunlight expose of those dyestuffs (0-25 min), and (0-12 min) for super oxide (O-2) production were studied. However benzanthrone produces detectable amount of1O2, Although metanil yellow andp-aminodiphenylamine (p-ADPA) did not produce detectable amounts of1O2under similar conditions. The above dye stuffs are routinely used in textiles, cosmetics, detergents, leather industries as well as food additives and role of these activated oxygen species in the development of skin diseases.


Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1148 ◽  
Author(s):  
Anton Misak ◽  
Lucia Kurakova ◽  
Eduard Goffa ◽  
Vlasta Brezova ◽  
Marian Grman ◽  
...  

Doxycycline (DOXY) is an antibiotic routinely prescribed in human and veterinary medicine for antibacterial treatment, but it has also numerous side effects that include oxidative stress, inflammation, cancer or hypoxia-induced injury. Endogenously produced hydrogen sulfide (H2S) and polysulfides affect similar biological processes, in which reactive oxygen species (ROS) play a role. Herein, we have studied the interaction of DOXY with H2S (Na2S) or polysulfides (Na2S2, Na2S3 and Na2S4) to gain insights into the biological effects of intermediates/products that they generate. To achieve this, UV-VIS, EPR spectroscopy and plasmid DNA (pDNA) cleavage assay were employed. Na2S or Na2S2 in a mixture with DOXY, depending on ratio, concentration and time, displayed bell-shape kinetics in terms of producing/scavenging superoxide and hydroxyl radicals and decomposing hydrogen peroxide. In contrast, the effects of individual compounds (except for Na2S2) were hardly observable. In addition, DOXY, as well as oxytetracycline and tetracycline, interacting with Na2S or other studied polysulfides reduced the •cPTIO radical. Tetracyclines induced pDNA cleavage in the presence of Na2S. Interestingly, they inhibited pDNA cleavage induced by other polysulfides. In conclusion, sulfide and polysulfides interacting with tetracyclines produce/scavenge free radicals, indicating a consequence for free radical biology under conditions of ROS production and tetracyclines administration.


Nanoscale ◽  
2018 ◽  
Vol 10 (15) ◽  
pp. 6971-6980 ◽  
Author(s):  
V. Baldim ◽  
F. Bedioui ◽  
N. Mignet ◽  
I. Margaill ◽  
J.-F. Berret

Cerium oxide nanoparticles are known to catalyze the decomposition of reactive oxygen species such as the superoxide radical and hydrogen peroxide.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 455E-455
Author(s):  
Shiow Y. Wang ◽  
Hongjun Jiao

The effect of blackberries (Rubus sp.) genotypes on antioxidant activities against superoxide radicals (O2–), hydrogen peroxide (H2O2), hydroxyl radicals (OH), and singlet oxygen (O,), was evaluated. The results were expressed as percent inhibition of active oxygen species production in the presence of fruit juice. The active oxygen radical absorbance capacity (ORAC) value referred to the net protection in the presence of fruit juice, and was expressed as micromoles of α-tocopherol, ascorbate, α-tocopherol, and β-carotene equivalents per 10 g of fresh weight for O2–, H2O2, OH, and O2, respectively. Among the different cultivars, juice of Hull' blackberry had the highest oxygen species, superoxide radicals (O2–), hydrogen peroxide (H2O2), hydroxyl radicals (OH), and singlet oxygen (O2,) scavenging capacity. Different antioxidants have their functional scavenging capacity against active oxygen species. There were interesting and marked differences among the different antioxidants in their abilities to inhibit the different active oxygen species. β-carotene had by far the highest scavenging activity against O2– but had absolutely no effect on H2O2. Ascorbic acid was the best at inhibiting H2O2 free radical activity. For OH, there was a wide range of scavenging capacities with α-tocopherol the highest and ascorbic acid the lowest. Glutathione had higher O2– scavenging capacity compared to the other antioxidants.


1984 ◽  
Vol 224 (3) ◽  
pp. 697-701 ◽  
Author(s):  
J M C Gutteridge

The stimulatory effect of ferrous salts on the peroxidation of phospholipids can be enhanced by EDTA when the concentration of Fe2+ in the reaction is greater than that of EDTA. Hydroxyl-radical scavengers do not inhibit peroxidation until the concentrations of Fe2+ and EDTA in the reaction are equal. Lipid peroxidation is then substantially initiated by hydroxyl radicals derived from a Fenton-type reaction requiring hydrogen peroxide. Superoxide radicals appear to play some role in the formation of initiating species.


2013 ◽  
Vol 41 (02) ◽  
pp. 425-441 ◽  
Author(s):  
Tong Ho Kang ◽  
Bin Na Hong ◽  
Su-Young Jung ◽  
Jeong-Han Lee ◽  
Hong-Seob So ◽  
...  

Cisplatin is commonly used as a chemotherapeutic agent against many human cancers. However, it generates reactive oxygen species (ROS) and has serious dose-limiting side effects, including ototoxicity. The roots of Curculigo orchioides (C. orchioides) have been used to treat auditory diseases such as tinnitus and hearing loss in Chinese traditional medicine. In the present study, we investigated the protective effects of an ethanol extract obtained from C. orchioides rhizome (COR) on cisplatin-induced cell damage in auditory cells (HEI-OC1). COR (2.5–25 μg/ml) inhibited cisplatin-induced HEI-OC1 cell damage in a dose-dependent manner. To investigate the protective mechanism of COR on cisplatin cytotoxicity in HEI-OC1 cells, we measured the effects of COR on ROS generation and lipid peroxidation in cisplatin-treated cells as well as its scavenging activities against superoxide radicals, hydroxyl radicals, hydrogen peroxide, and DPPH radicals. COR (1–25 μg/ml) had scavenging activities against superoxide radicals, hydroxyl radicals, hydrogen peroxide, and DPPH radicals, as well as reduced lipid peroxidation. In in vivo experiments, COR was shown to reduce cochlear and peripheral auditory function impairments through cisplatin-induced auditory damage in mice. These results indicate that COR protects from cisplatin-induced auditory damage by inhibiting lipid peroxidation and scavenging activities against free radicals.


2007 ◽  
Vol 34 (12) ◽  
pp. 1112 ◽  
Author(s):  
Iva Šnyrychová ◽  
Péter B. Kós ◽  
Éva Hideg

The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (•OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation of terephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm–2), •OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B-driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of •OH was still observed in thylakoids, even though no free hydrogen peroxide was detected. At this late stage of UV-B stress, •OH may also be formed by the direct cleavage of organic peroxides by UV-B. Immunodetection showed that the presence of the observed ROS alone was not sufficient to achieve the degradation of the D1 protein of PSII centres.


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