EYE ON CHINA

2017 ◽  
Vol 21 (09) ◽  
pp. 4-7
Keyword(s):  
Brain Imaging ◽  
High Throughput ◽  
Neural Circuit ◽  
Organ Transplant ◽  
Treatment Center ◽  
Human Organ ◽  
Global Innovation ◽  
Itch Sensation ◽  
Body Sensors ◽  
Set Up

High-throughput brain imaging institute to be set up in Suzhou. Breakthrough in pig-to-human organ transplant. Scientists identify central neural circuit for itch sensation. Silk-based wearable body sensors developed by Tsinghua researchers. First AI-assisted treatment center in Hefei city. China moves higher in Global Innovation Index.

BioBoard

2007 ◽  
Vol 11 (06) ◽  
pp. 318-328
Keyword(s):  
Hong Kong ◽  
New Zealand ◽  
South Korea ◽  
Organ Transplant ◽  
Science Research ◽  
Human Case ◽  
Human Organ ◽  
Technological Infrastructure ◽  
Draft Regulation ◽  
Set Up

Australia — Australia and New Zealand Collaborate on Grain Research. Australia — Cervical Cancer Hope for Developing World. Australia — Agilent Technologies Honors Australian Researcher with 2007 Manfred Donike Award. China — China Adopts Five-Year Health Plan. China — Chinese HIV/AIDS Advocate Gao Receives Award. China — China's New Rule on Prescription of Drugs. China — China to Increase Spending on Agricultural Biotechnology. China — China to Increase Natural Science Research Investment. China — Free TB Detection and Treatment Promised in China. China — Dr Zhang Xu Awarded the 6th Shanghai Peony Award for Research in Natural Sciences. China — National Survey on TB Drug-Resistance to Be Launched. China — Twelve Technological Infrastructure Projects to Be Built in China. China — China Passes Draft Regulation on Human Organ Transplant. China — Scientist Wins Top Scientific and Technological Award. China — Science and Technology Progress in 2006. Hong Kong — Hong Kong Reports Human Case of H9N2. India — India's Maharashtra FDA Conducted Raids on Fake Drug Makers. India — NIH Chooses India and China for HIV Clinical Trials. India — Apollo Hospitals Teams Up with StemCyte on Stem Cell Research. India — Ranbaxy in Lipitor Patent Suit with Pfizer in 17 countries. India — DuPont to Set Up R&D Center in India. India — AZRFI Dissolved and AstraZeneca to Mentor Science Sponsorship. India — AstraZeneca's New Center in India. Japan — Tamiflu May be Dangerous to Teens. New Zealand — PHARMAC Asking about Herceptin Funding. New Zealand — Two New Medicines Funded for People with HIV. South Korea — South Korean Laboratory Develops Radiation Source for Cancer Treatment. South Korea — Health Ministers of South Korea, China and Japan to Meet. South Korea — South Korea to Issue Safety Warnings on Tamiflu. Taiwan — Taiwan Scientists Develop Reproductive Cells from hESC. Thailand — Thai Health Groups Urge the Boycotting of Abbott. Vietnam — Vietnam to Produce Bird Flu Vaccines for Poultry. Others — WHO takes Stern Measures against Fake Drugs.

scholarly journals Chemical sectioning fluorescence tomography: high-throughput, high-contrast, multicolor, whole-brain imaging at subcellular resolution

Cell Reports ◽  
2021 ◽  
Vol 34 (5) ◽  
pp. 108709
Author(s):  
Xiaojun Wang ◽  
Hanqing Xiong ◽  
Yurong Liu ◽  
Tao Yang ◽  
Anan Li ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Throughput ◽  
High Contrast ◽  
Whole Brain ◽  
Fluorescence Tomography ◽  
Subcellular Resolution

scholarly journals Automatic 1D 1H NMR Metabolite Quantification for Bioreactor Monitoring

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 157
Author(s):  
Roy Chih Chung Wang ◽  
David A. Campbell ◽  
James R. Green ◽  
Miroslava Čuperlović-Culf
Keyword(s):  
High Throughput ◽  
Nmr Metabolomics ◽  
Gene Therapies ◽  
Quantification Method ◽  
Minimal Sample ◽  
Enhanced Production ◽  
Bioreactor Monitoring ◽  
Online Measurements ◽  
Metabolite Quantification ◽  
Set Up

High-throughput metabolomics can be used to optimize cell growth for enhanced production or for monitoring cell health in bioreactors. It has applications in cell and gene therapies, vaccines, biologics, and bioprocessing. NMR metabolomics is a method that allows for fast and reliable experimentation, requires only minimal sample preparation, and can be set up to take online measurements of cell media for bioreactor monitoring. This type of application requires a fully automated metabolite quantification method that can be linked with high-throughput measurements. In this review, we discuss the quantifier requirements in this type of application, the existing methods for NMR metabolomics quantification, and the performance of three existing quantifiers in the context of NMR metabolomics for bioreactor monitoring.

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

Data Processing Methods for a High Throughput Brain Imaging PET Research Center

2006 ◽  
Author(s):  
Judson P. Jones ◽  
Arman Rahmim ◽  
Merence Sibomana ◽  
Andrew Crabb ◽  
Ziad Burbar ◽  
...  
Keyword(s):  
Data Processing ◽  
Brain Imaging ◽  
High Throughput ◽  
Research Center ◽  
Processing Methods ◽  
Data Processing Methods

Modular automated bottom-up proteomic sample preparation for high-throughput applications v1

2021 ◽  
Author(s):  
Yan Chen ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Tad Ogorzalek ◽  
Paul D. Adams ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Tryptic Digestion ◽  
Gram Negative Bacteria ◽  
Bottom Up ◽  
Preparation Methods ◽  
Gram Negative ◽  
E Coli ◽  
Protocol Development ◽  
Set Up

Manual proteomic sample preparation methods limit sample throughput and often lead to poor data quality when thousands of samples must be analyzed. Automated workflows are increasingly used to overcome these issues for some (or even all) of the sample preparation steps. Here, we detail three optimised step-by-step protocols to: (A) lyse Gram-negative bacteria and fungal cells; (B) quantify the amount of protein extracted; and (C) normalize the amount of protein and set up tryptic digestion. These protocols have been developed to facilitate rapid, low variance sample preparation of hundreds of samples, be easily implemented on widely-available Beckman-Coulter Biomek automated liquid handlers, and allow flexibility for future protocol development. By using this workflow 50 micrograms of peptides for 96 samples can be prepared for tryptic digestion in under an hour. We validate these protocols by analyzing 47 E. coli and R. toruloides samples and show that this modular workflow provides robust, reproducible proteomic samples for high-throughput applications. The expected results from these protocols are 94 peptide samples from Gram-negative bacterial and fungal cells prepared for bottom-up quantitative proteomic analysis without the need for desalting column cleanup and with peptide variance (CVs) below 15%.

Joseph Murray: Pioneering Plastic Surgeon and Father of the First Organ Transplant

2021 ◽  
pp. 000313482110488
Author(s):  
Shruthi Deivasigamani ◽  
Benjamin Phillips ◽  
Charles J. Yeo ◽  
Renee M. Tholey
Keyword(s):  
World War Ii ◽  
Organ Transplant ◽  
Plastic Surgeon ◽  
Unrelated Donor ◽  
Human Organ ◽  
Us Army ◽  
Burn Victims ◽  
Graft Donor ◽  
Peter Bent Brigham Hospital ◽  
Valley Forge

Dr. Joseph Murray was a plastic surgeon who is best known for performing the first successful human organ transplant. After graduating from Harvard Medical School and completing a surgical internship at Peter Bent Brigham Hospital, Murray enlisted in the US Army Medical Corp and spent 5 years at Valley Forge General Hospital treating World War II soldiers injured in combat. He treated hundreds of burn victims with skin grafts and took an interest in the variable process of graft rejection based on both the patient’s relation to the graft donor and the patient’s level of immunocompetency. His work at Valley Forge set the stage for his research investigating the feasibility of kidney transplantation and immunosuppression. He went on to perform the first successful kidney transplant between identical twins in 1954, between fraternal twins in 1959, and between an unrelated donor and recipient in 1962. For his efforts, he was awarded the 1990 Nobel Prize in Medicine.

New Strategies for Prevention and Therapy of Cytomegalovirus Infection and Disease in Solid-Organ Transplant Recipients

2000 ◽  
Vol 13 (1) ◽  
pp. 83-121 ◽  
Author(s):  
Irene G. Sia ◽  
Robin Patel
Keyword(s):  
Antiviral Agents ◽  
Organ Transplant ◽  
Solid Organ Transplant ◽  
Solid Organ ◽  
Cmv Infection ◽  
Human Organ ◽  
Preventative Measures ◽  
Daunting Task ◽  
History Of ◽  
Antiviral Chemotherapy

SUMMARY In the past three decades since the inception of human organ transplantation, cytomegalovirus (CMV) has gained increasing clinical import because it is a common pathogen in the immunocompromised transplant recipient. Patients may suffer from severe manifestations of this infection along with the threat of potential fatality. Additionally, the dynamic evolution of immunosuppressive and antiviral agents has brought forth changes in the natural history of CMV infection and disease. Transplant physicians now face the daunting task of recognizing and managing the changing spectrum of CMV infection and its consequences in the organ recipient. For the microbiology laboratory, the emphasis has been geared toward the development of more sophisticated detection assays, including methods to detect emerging antiviral resistance. The discovery of novel antiviral chemotherapy is an important theme of clinical research. Investigations have also focused on preventative measures for CMV disease in the solid-organ transplant population. In all, while much has been achieved in the overall management of CMV infection, the current understanding of CMV pathogenesis and therapy still leaves much to be learned before success can be claimed.

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