Synthesis, properties and photodynamic activities of some zinc(II) phthalocyanines against Escherichia coli and Staphylococcus aureus

2008 ◽  
Vol 12 (08) ◽  
pp. 953-963 ◽  
Author(s):  
Ozlem Seven ◽  
Bircan Dindar ◽  
Sohret Aydemir ◽  
Feriha Cilli
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Gram Negative Bacteria ◽  
Bacterial Inactivation ◽  
Gram Negative ◽  
Oxygen Generation ◽  
Gram Positive Bacteria ◽  
Singlet Oxygen Generation

Two new zinc phthalocyanine derivatives bearing four 3,5-di-tert-butyl-4-hydroxyphenyl (ZnPc1) and 3,5-dimethylphenoxy (ZnPc2) have been synthesized and proved by elemental analyses and UV-vis, 1H NMR, FTIR and MALDI-TOF mass spectra as spectroscopic determination. Thermal stabilities of these neutral Zn -phthalocyanines were performed by thermal gravimetric analysis and, significantly, were found stable up to 373 °C for ZnPc1 and 550 °C for ZnPc2. In addition, the photostability of the sensitizers was quite successful within 240 min. Furthermore, photodynamic therapy has been investigated using these neutral phthalocyanines. Singlet oxygen generation capacities of ZnPc1 and ZnPc2 were studied using 1,3-diphenyl-iso-benzofuran (50.0 μM) as a selective singlet oxygen trap in DMSO and both of them demonstrated very high singlet oxygen generation capacity. Photodynamic therapy is of considerable interest for its potential as an antimicrobial therapy on the grounds that the photodynamic activity of these compounds was tested against a Gram-negative bacteria, Escherichia coli and a Gram-positive bacteria, Staphylococcus aureus. Suspensions of the microorganisms were irradiated for 240 min in the presence of ZnPc1 (with hydrophilic group) and ZnPc2 phthalocyanines. In order to simulate solar radiation, we used a 750 W xenon lamp. Minimum photosensitizer concentration was used as 0.1 mg.10 mL-1 in 1% dimethylsulfoxide/phosphate buffer saline solution (DMSO/PBS). Moreover, the photostability of these compounds has been investigated and the effect of the amount of DMSO tested against selected bacteria. In the dark, with and without Pcs, bacterial inactivation did not occur. Bacterial inactivation by light with ZnPc1 was observed in response to Gram-negative bacteria E. coli and Gram-positive bacteria S. aureus, whereas the photoinactivation studies with ZnPc2 have revealed that the lack of its activity is due to its poor affinity for either of the organisms. These results suggest that a neutral amphiphilic photosensitizer may be easily used in an application concerning photoinactivation of bacterial cells as well as ionic photosensitizers.

scholarly journals Water-dispersible glycosylated poly(2,5′-thienylene)porphyrin-based nanoparticles for antibacterial photodynamic therapy

2019 ◽  
Vol 18 (5) ◽  
pp. 1147-1155 ◽  
Author(s):  
Rehan Khan ◽  
Melis Özkan ◽  
Aisan Khaligh ◽  
Dönüs Tuncel
Keyword(s):  
Antibacterial Activity ◽  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Water Dispersible ◽  
Gram Positive Bacteria ◽  
High Yields

Water-dispersible glycosylated poly(2,5′-thienylene)porphyrin-based nanoparticles have the ability to generate singlet oxygen in high yields and exhibit light-triggered antibacterial activity against Gram negative bacteria, E. coli as well as Gram positive bacteria, B. subtilis.

Survival of some non-starter bacteria in naturally ripened and enzyme-accelerated Cheddar cheese

1988 ◽  
Vol 55 (4) ◽  
pp. 597-602 ◽  
Author(s):  
Lydia Bautista ◽  
Rohan G. Kroll
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Enzyme System ◽  
Cheddar Cheese ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ripening Period ◽  
Gram Positive Bacteria

SummaryEffects of the addition of a proteinase (Neutrase 1–5S) and a peptidase (aminopeptidase DP-102) as agents for accelerating the ripening of Cheddar cheese on the survival of some non-starter bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coliand aSalmonellasp.) were studied throughout a 4-month ripening period. The enzymes were found to have no significant effect on the survival of the Gram-positive bacteria but some significant effects were observed, at some stages of the ripening period, with the Gram-negative bacteria in that lower levels were recovered from cheeses treated with the enzyme system.

scholarly journals Paper modified with ZnO nanorods – antimicrobial studies

2012 ◽  
Vol 3 ◽  
pp. 684-691 ◽  
Author(s):  
Mayuree Jaisai ◽  
Sunandan Baruah ◽  
Joydeep Dutta
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Aspergillus Niger ◽  
Zno Nanorods ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Tissue Paper ◽  
Gram Positive Bacteria ◽  
Lighting Conditions

Paper with antimicrobial properties was developed through in situ growth of ZnO nanorods. The targeted application for this type of paper is in health centers as wallpaper, writing paper, facemasks, tissue paper, etc. The paper was tested on three model microbes, Gram-positive bacteriaStaphylococcus aureus,Gram-negative bacteriaEscherichia coliand common airborne fungusAspergillus niger. No viable bacterial colonies or fungal spores could be detected in the areas surrounding test samples of the antimicrobial paper. Gram-negative bacteriaEscherichia coliwere found to be inhibited in an area that is 239% and 163% the area of the paper sample under different room lighting conditions, i.e., halogen and fluorescent lamp illumination, respectively. For Gram-positive bacteriaStaphylococcus aureusthe zones of inhibition surrounding the paper samples are 102% and 70%, and forAspergillus niger, 224% and 183% of the sample area, under similar lighting conditions.

Composition and Antimicrobial Activity of the Essential Oil of a Wild Kiwi

2011 ◽  
Vol 322 ◽  
pp. 160-163
Author(s):  
Yin Lu ◽  
Hong Chen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Fungal Species ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Leaf Oil ◽  
First Time

A medicinal wild kiwi in China, Actinidia valvata Dunn, has been well known for its activities against leprosy and cancers. The compositions and the antimicrobial activity of its leaf oil were reported for the first time. The oil obtained by hydrodistillation and analyzed by GC and GC-MS, was characterized by the high content of monoterpenes. Linalool (48.14%) is the major component identified, followed by 1,2-dimethyl-lindoline (7.94%), linolenic acid methylester (6.57%) and (E)-phytol (5.29%). The antimicrobial activity of the oil was evaluated against four bacterial and three fungal species. The results showed that it exhibited a mild antibacterial activity against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), a significant activity against Gram-negative bacteria (Escherichia coli), and no activity on Pseudomonas aeruginosa. The test fungi were more sensitive to the oil, with a MIC range of 0.78~1.56 μL/mL than bacteria in the range which were significantly higher from 0.78 to 25.50 μL/mL.

scholarly journals Green Synthesis, Antioxidant and Antimicrobial Activity of Silver Nanoparticles Using Gnetum africanum Extracts

2021 ◽  
pp. 1-7
Author(s):  
I. M. Uneze ◽  
J. O. Otonko ◽  
A. K. Adigun ◽  
S. J. Adebayo
Keyword(s):  
Escherichia Coli ◽  
Aqueous Solution ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Positive Bacteria

The synthesis and application of nanoparticles is an important area of research that is gaining attention recently. In this recent project, we report the synthesis of silver nanoparticles, AgNP using aqueous solution of silver nitrate and Gnetum africanum leaf extract (reducing agent). The synthesis of AgNP was achieved by mixing aqueous solution of silver nitrate (70ml, 15.75mM) with a solution of Gnetum africanum leaf extract 100 ml) in a reaction flask and allowed to stand for 24 hours in a dark cupboard. A color change from light brown to yellowish brown was observed which indicated that synthesis of silver nanoparticles took place. The presence of AgNP was ascertained using UV-vis spectra analysis and absorption at 442 nm showed the presence of AgNP. The antioxidant assay of both the synthesized AgNP and the leaf extract was determined using DPPH. Antimicrobial activity was conducted using three different organisms which were Staphylococcus aureus, Escherichia coli and Pseudomonas respectively. The antioxidant results using DPPH scavenging ability of AgNp showed that at concentrations of 2mg/ml,1mg/ml and 0.1mg/ml, the percentage inhibition  of  DPPH  by AgNp was 61.69, 53.06 and 38.31 respectively and that of Gnetum africanum leaf extract was 81.32, 78.49, and 58.29 respectively at the same concentrations using Ascorbic acid as a standard. The antimicrobial activity of both the synthesized AgNps and Gnetum Africanum Leaf extract using one gram positive bacteria (Staphylococcus aureus) and two gram negative bacteria (Escherichia coli and Pseudomonas) revealed that the synthesized AgNps showed lesser activity than Gnetumafricanum leaf extract for both the gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Pseudomonas) and (Escherichia coli). From the above findings, it can be observed that Gnetum Africanum Leaf extract reduced Ag+ to Ag0 and also both the synthesized AgNps and the Gnetum Africanum Leaf extract showed reasonable antioxidant activity against DPPH and antimicrobial activity against the tested microorganisms. This implied that both samples have medicinal values.

scholarly journals Analysis of antibiotics administration in diabetic ulcer patients at Panembahan Senopati regional hospital Bantul

2021 ◽  
Vol 8 (8) ◽  
pp. 3782
Author(s):  
Adnal K. P. Husein Putra ◽  
Sri Sundari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Antibiotic Administration ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Diabetic Ulcer ◽  
Gram Negative ◽  
Gram Positive Bacteria

Background: This study provides an overview of diabetic ulcer infection, antibiotic susceptibility patterns, the most common types of antibiotics, factors influence in antibiotics administrations, and verifying the guideline used of antibiotics.Methods: The research used descriptive analysis combined with interviews. The study was starting from February-March 2021. Eighty-six diabetic ulcer patients were selected considering the inclusion and exclusion criteria. We interviewed seven doctors.Results: 71 bacterias were found with a gram-negative bacteria count of 80.3%- higher than gram-positive bacteria (19.7%). The most common gram-negative bacteria found are Escherichia coli (12.7%) and show the most heightened sensitivity with imipenem (80.7%), while cefuroxime and trimethoprim-sulfamethoxazole show the highest resistance (64.9%). The most common gram-positive bacteria found are Staphylococcus aureus (14.1%), and vancomycin shows the most heightened sensitivity (100%), while penicillin shows the highest resistance (71.4%). The most common single antibiotic administration was ceftriaxone, as well as a combination of two antibiotics, namely ceftriaxone and metronidazole.Conclusions: Escherichia coli was the most gram-negative bacteria, which has the highest sensitivity with imipenem, while Staphylococcus aureus was the most gram-positive bacteria, which has the most heightened sensitivity with vancomycin. The most common single antibiotic administration was ceftriaxone, as well as a combination of two antibiotics, ceftriaxone and metronidazole. Factors that influence antibiotics administration are patient condition, susceptibility testing, training, and advice from colleagues. Every doctor uses different guidelines for diabetic foot ulcer.

Green Synthesis of Novel ethyl 3-amino-5-(methylthio)-4-(5-substituted phenyloxazol-2-yl)thiophene-2-carboxylate derivatives

2021 ◽  
pp. 105-110
Author(s):  
Satish M. Chavan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Microwave Irradiation ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Green Protocol ◽  
Gram Positive Bacteria

Synthesis of novel ethyl 3-amino-5-(methylthio)-4-(5-substituted phenyloxazol-2-yl) thiophene-2-carboxylate derivatives from 3-amino-4-carbamoyl-5-(methylthio)thiophene-2-carboxylate was carried out by traditional as well as microwave irradiation green protocol. Ethyl 3-amino-5-(methylthio)-4-(5-substituted phenyloxazol-2-yl) thiophene-2-carboxylate derivatives were screened for antimicrobial screening against gram positive bacteria Staphylococcus aureus (ATCC 29737), gram negative bacteria Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (MTCC 277), Aspergillus niger (MCIM 545) fungi.

scholarly journals Reactions of tin- and triorganotin(IV) isopropoxides with thymol derivative: Synthesis, characterization and in vitro antimicrobial screening

2013 ◽  
Vol 78 (9) ◽  
pp. 1323-1333 ◽  
Author(s):  
Garima Matela ◽  
Robina Aman ◽  
Chetan Sharma ◽  
Smita Chaudhary
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Nuclear Magnetic Resonance ◽  
Antibacterial Activity ◽  
Gram Negative Bacteria ◽  
Carboxylate Group ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
1H Nuclear Magnetic Resonance

A new series of diisopropyloxytin- and triorganotin(IV) complexes of H2hbgl (1) of the general formula Sn(OPri)2(hbgl) (2), Sn(OPri)2(Hhbgl)2 (3), Ph3Sn(Hhbgl) (4), Bu3Sn(Hhbgl) (5) and Me3Sn(Hhbgl) (6), [where H2hbgl= a ligand of thymol derivative namely, N-(2-hydroxy-3-isopropyl-6-methyl benzyl)Glycine] were synthesized by reacting tin- and triorganotin(IV) chloride with the ligand, with the aid of sodium iso-propoxide in appropriate stiochiometric ratios (1:1 and 1:2). These complexes were characterized by elemental analysis, IR, 1H nuclear magnetic resonance. The spectral data suggest that the carboxylate group, in complexes 2-5, was bonded in a bidentate manner, while a unidentate bonding was observed in complex 6. All five complexes were tested in vitro for their antibacterial activity against Gram-positive bacteria namely, Staphylococcus aureus MTCC 96, Bacillus subtilis MTCC 121 and two Gram-negative bacteria namely, Escherichia coli MTCC 1652 and Pseudomonas aeruginosa MTCC 741. All the five complexes were also tested against three pathogenic fungal strains namely, Aspergillus niger, A. flavus and Penicillium sp.

Enhancement of photobactericidal activity of chlorin-e6-cellulose nanocrystals by covalent attachment of polymyxin B

2017 ◽  
Vol 5 (33) ◽  
pp. 6953-6962 ◽  
Author(s):  
Florent Le Guern ◽  
Tan-Sothea Ouk ◽  
Karine Grenier ◽  
Nicolas Joly ◽  
Vincent Lequart ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Polymyxin B ◽  
Chlorin E6 ◽  
Covalent Attachment ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Positive Bacteria

Following light irradiation, a new nanomaterial, elaborated from CNCs, chlorin-e6 and polymyxin B, demonstrated efficiency against Gram-negative bacteria (Escherichia coli,Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus,Staphylococcus epidermidis).

scholarly journals Gram Negative Bacteria (Escherichia coli) Win Against Gram Positive Bacteria (Staphylococcus aureus) in The Same Media

2021 ◽  
Vol 4 (2) ◽  
pp. 113
Author(s):  
Neisya Intan Cahyaningtyas Agung Putri ◽  
Ramadhani Ramadhani ◽  
Eddy Bagus Wasito
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Doubling Time ◽  
Bacterial Culture ◽  
Agar Plate ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Significant Difference

Introduction: Biodiversity of the microorganism in Indonesia lead to the large amount of patient with infection. Human can get infected in two different place, with different kind of bacteria that cause the infection. This may lead to bacteremia without knowing which bacteria type whose causing it, either the Gram positive or Gram negative bacteria, whereas the treatment of this two types of bacteria are different. The aim of this study is to determine the doubling time of the Gram positive and Gram negative bacteria when they are grown in the same lesion and the kinds of bacteria that we need to eliminate first.Methods: Staphylococcus aureus and Escherichia coli bacteria were used as samples in this study. Bacterial culture in nutrient broth with 0.5 OD turbidity were mixed then incubated in incubator with 35˚C. Every one hour within 24 hour, 0.01 ml of bacterial culture was taken in serial dilutionover time, varying between 106 – 1012, . It was then planted in nutrient agar plate with droplets technique. After it had been incubated for 24 hours, we counted the Colony Forming Unit per ml (CFU/ml) to time, then the doubling time of the bacteria. The result were then compared between the Staphylococcus aureus and Escherichia coli group.Results: Two tailed t-test result of the doubling time between Staphylococcus aureus dan Escherichia coli was < 0,05 (p=0,000) wich means that there is significant difference of the doubling time between Staphylococcus aureus (24,35 ± 2,23 munites), and Escherichia coli (18,37 ± 0,50 minutes). When grown in the same media, Gram positive bacteria (Staphylococcus aureus) had slower doubling time than Gram negative bacteria (Escherichia coli) as much as 1.32 times.Conclusion: In bacteremia with two possible kinds of bacterial suspect, we need to eliminate the Gram negative bacteria first.

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