Crop Improvement Through Temperature Resilience

2019 ◽  
Vol 70 (1) ◽  
pp. 753-780 ◽  
Author(s):  
Jingyu Zhang ◽  
Xin-Min Li ◽  
Hong-Xuan Lin ◽  
Kang Chong
Keyword(s):  
Crop Production ◽  
Molecular Mechanisms ◽  
Crop Improvement ◽  
Hybrid Breeding ◽  
Plant Responses ◽  
Temperature Sensitive ◽  
Heat Stress Response ◽  
Signal Perception ◽  
Temperature Signal

Abnormal environmental temperature affects plant growth and threatens crop production. Understanding temperature signal sensing and the balance between defense and development in plants lays the foundation for improvement of temperature resilience. Here, we summarize the current understanding of cold signal perception/transduction as well as heat stress response. Dissection of plant responses to different levels of cold stresses (chilling and freezing) illustrates their common and distinct signaling pathways. Axillary bud differentiation in response to chilling is presented as an example of the trade-off between defense and development. Vernalization is a cold-dependent development adjustment mediated by O-GlcNAcylation and phosphorylation to sense long-term cold. Recent progress on major quantitative trait loci genes for heat tolerance has been summarized. Molecular mechanisms in utilizing temperature-sensitive sterility in super hybrid breeding in China are revealed. The way to improve crop temperature resilience using integrative knowledge of omics as well as systemic and synthetic biology, especially the molecular module program, is summarized.

scholarly journals Transcriptome Analysis Reveals Photoperiod-Associated Genes Expressed in Rice Anthers

2021 ◽  
Vol 12 ◽  
Author(s):  
Shiyu Sun ◽  
Duoxiang Wang ◽  
Jingbin Li ◽  
Yaqi Lei ◽  
Gang Li ◽  
...  
Keyword(s):  
Male Fertility ◽  
Regulatory Networks ◽  
Molecular Mechanisms ◽  
Crop Improvement ◽  
Differentially Expressed ◽  
Hybrid Breeding ◽  
Temperature Sensitive ◽  
Male Sterile ◽  
Time Points ◽  
Photoperiod Sensitive

Environmental conditions, such as photoperiod and temperature, can affect male fertility in plants. While this feature is heavily exploited in rice to generate male-sterile lines for hybrid breeding, the underlying molecular mechanisms remain largely unknown. In this study, we use a transcriptomics approach to identify key genes and regulatory networks affecting pollen maturation in rice anthers in response to different day lengths. A total of 11,726 differentially expressed genes (DEGs) were revealed, of which 177 were differentially expressed at six time points over a 24-h period. GO enrichment analysis revealed that genes at all time points were enriched in transport, carbohydrate, and lipid metabolic processes, and signaling pathways, particularly phytohormone signaling. In addition, co-expression network analysis revealed four modules strongly correlated with photoperiod. Within these four modules, 496 hub genes were identified with a high degree of connectivity to other photoperiod-sensitive DEGs, including two previously reported photoperiod- and temperature-sensitive genes affecting male fertility, Carbon Starved Anther and UDP-glucose pyrophosphorylase, respectively. This work provides a new understanding on photoperiod-sensitive pollen development in rice, and our gene expression data will provide a new, comprehensive resource to identify new environmentally sensitive genes regulating male fertility for use in crop improvement.

scholarly journals Efficient Method of Transporting Coconut (Cocos nucifera L.) Zygotic Embryos for Cryopreservation of Plumules by Encapsulation/Dehydration

CORD ◽  
2014 ◽  
Vol 30 (2) ◽  
pp. 12
Author(s):  
H D D Bandupriya
Keyword(s):  
Crop Production ◽  
Cocos Nucifera ◽  
Crop Improvement ◽  
Germplasm Collection ◽  
Zygotic Embryos ◽  
Germplasm Storage ◽  
Germplasm Exchange ◽  
Long Term Storage ◽  
Term Storage

Coconut is both socially and economically important crop in tropical and subtropical countries, thus the conservation of existing diversity of its germplasm is vital to maintain biodiversity, sustain crop production and utilisation of germplasm for crop improvement strategies. The recalcitrant storage behavior and large size of the coconut seed make it impossible to use as a germplasm storage material. Cryopreservation is an ideal means of long-term storage of germplasm which offers long-term storage capability with minimal storage space and maintenance requirements. The coconut embryo has been now adapted by various researchers for the purpose of germplasm exchange and it is now being routinely applied in germplasm collection and exchange activities with sufficient germination rates. The aim of the present study was to determine the effect of different coconut embryo transport/ storage methods [as solid endosperm plugs under cold temperature, embryos cultured in Solidified Agar Medium (SAM) or KCl solution under room temperature] on cryopreservation of plumules using encapsulation/dehydration method. The results revealed that plumules excised from embryos transported/ stored in SAM and pretreated with 1.0M sucrose could be cryopreserved with 71.8% survival and 56% recovery rates. The survival and recovery could be further increased up to 77.5% and 65% respectively by supplementation of 1.0M sucrose with 20 µM ABA.

scholarly journals A temperature sensitive mutation in the CstF77 subunit of the polyadenylation complex reveals the critical function of mRNA 3' end formation for a robust heat stress response in plants

2021 ◽  
Author(s):  
Minsoo Kim ◽  
John D Swenson ◽  
Fionn McLoughlin ◽  
Elizabeth Vierling
Keyword(s):  
Heat Stress ◽  
Molecular Mechanisms ◽  
Dominant Role ◽  
Temperature Sensitive ◽  
Heat Sensitivity ◽  
Critical Function ◽  
Heat Stress Response ◽  
Transcript Processing ◽  
Cleavage Stimulation Factor ◽  
Inducible Genes

Background: Heat Shock Protein 101 (HSP101) in plants and orthologs in bacteria (Caseinolytic peptidase B, ClpB) and yeast (Hsp104) are essential for thermotolerance. To investigate molecular mechanisms of thermotolerance involving HSP101, we performed a suppressor screen in Arabidopsis thaliana of a semi-dominant, missense HSP101 allele, hot1-4 (A499T). Plants carrying the hot1-4 mutation are more heat-sensitive than an HSP101 null mutant (hot1-3), indicating the toxicity of hot1-4 allele. Results: We report that one suppressor (shot2, suppressor of hot1-4 2) has a temperature-sensitive, missense mutation (E170K) in the CstF77 (Cleavage stimulation factor 77) subunit of the polyadenylation complex, which is critical for 3' end maturation of pre-mRNA. RNA-Seq analysis of total RNA depleted of ribosomes reveals that heat treatment causes transcriptional readthrough events in shot2, specifically in highly heat-induced genes, including the toxic hot1-4 gene. In addition, failure of correct transcript processing leads to reduced accumulation of many HSP RNAs and proteins, suppressing heat sensitivity of the hot1-4 mutant, due to reduction of the toxic mutant HSP101 protein. Notably, the shot2 mutation makes plants more sensitive to heat stress in the HSP101 null (hot1-3) and wild-type backgrounds correlated with the reduced expression of other heat-inducible genes required for thermotolerance. Conclusions: Our study reveals the critical function of CstF77 for 3' end formation of mRNA during heat stress, as well as the dominant role of HSP101 in dictating the outcome of severe heat stress in plants.

scholarly journals High Chromosomal Stability and Immortalized Totipotency Characterize Long-Term Tissue Cultures of Chinese Ginseng (Panax Ginseng)

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 514
Author(s):  
Sitong Liu ◽  
Jing Zhao ◽  
Yutong Liu ◽  
Ning Li ◽  
Zhenhui Wang ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Chromosomal Instability ◽  
Molecular Mechanisms ◽  
Crop Improvement ◽  
Rapid Decline ◽  
Chromosomal Stability ◽  
Complex Phenotypes ◽  
Culture Duration ◽  
Transgenic Technologies

Chinese ginseng (Panax ginseng C. A. Meyer) is a highly cherished traditional Chinese medicine, with several confirmed medical effects and many more asserted health-boosting functions. Somatic chromosomal instability (CIN) is a hallmark of many types of human cancers and also related to other pathogenic conditions such as miscarriages and intellectual disabilities, hence, the study of this phenomenon is of wide scientific and translational medical significance. CIN also ubiquitously occurs in cultured plant cells, and is implicated as a major cause of the rapid decline/loss of totipotency with culture duration, which represents a major hindrance to the application of transgenic technologies in crop improvement. Here, we report two salient features of long-term cultured callus cells of ginseng, i.e., high chromosomal stability and virtually immortalized totipotency. Specifically, we document that our callus of ginseng, which has been subcultured for 12 consecutive years, remained highly stable at the chromosomal level and showed little decline in totipotency. We show that these remarkable features of cultured ginseng cells are likely relevant to the robust homeostasis of the transcriptional expression of specific genes (i.e., genes related to tissue totipotency and chromosomal stability) implicated in the manifestation of these two complex phenotypes. To our knowledge, these two properties of ginseng have not been observed in any animals (with respect to somatic chromosomal stability) and other plants. We posit that further exploration of the molecular mechanisms underlying these unique properties of ginseng, especially somatic chromosomal stability in protracted culture duration, may provide novel clues to the mechanistic understanding of the occurrence of CIN in human disease.

Transcriptional Profiling Reveals Adaptive Responses to Boron Defi ciency Stress in Arabidopsis

2012 ◽  
Vol 67 (9-10) ◽  
pp. 510-524
Author(s):  
Lishun Peng ◽  
Changying Zeng ◽  
Lei Shi ◽  
Hongmei Cai ◽  
Fangsen Xu
Keyword(s):  
Crop Production ◽  
Transcriptional Profiling ◽  
Global Gene Expression ◽  
Cellular Growth ◽  
Limiting Factor ◽  
Plant Responses ◽  
Adaptive Responses ◽  
Short And Long Term ◽  
Prominent Response

Boron (B) is a micronutrient for vascular plants, and B deficiency has been recognized as a limiting factor for crop production in many areas worldwide. To gain a better insight into the adaptability mechanism of plant responses to B starvation, an Arabidopsis whole genome Affymetrix GeneChip was used to evaluate global gene expression alterations in response to short- and long-term B deficiency stress. A large number of B deficiency-responsive genes were identified and grouped by their functions. Genes linked to jasmonic acid (JA) showed the most prominent response under B deficiency. The transcripts for biosynthesis and regulation of JA were constantly induced during short- and long-term B deficiency stress. A set of well-known JA-dependent process and responsive genes showed the same expression profile. This suggested that JA could be a pivotal player in the integration of adaptive responses to B deficiency stress. Moreover, other functional groups of B deficiency-responsive genes (including various encoding the biosynthesis of antioxidants, the basic components of Ca2+ signalling, protein kinases, cell wall-modifying enzymes and proteins, H+-ATPase, K+ transporters, and a set of enzymes involved in central metabolism and cellular growth) were also observed, and their physiological roles under B deficiency stress are discussed. These results provide some information for a better understanding of plant-adaptive responses to B deficiency stress and potential strategies to improve B efficiency in crops

Impact of long-term trials on crop production research and education

2010 ◽  
Vol 58 (Supplement 1) ◽  
pp. 1-5 ◽  
Author(s):  
M. Jolánkai ◽  
F. Nyárai ◽  
K. Kassai
Keyword(s):  
Crop Production ◽  
Life Sciences ◽  
Ecological Models ◽  
Dynamic Databases ◽  
Production Research ◽  
Research And Education

Long-term trials have a twofold role in life sciences, acting as both live laboratories and public collections. Long-term trials are not simply scientific curios or the honoured relics of a museum, but highly valuable live ecological models that can never be replaced or restarted if once terminated or suspended. These trials provide valuable and dynamic databases for solving scientific problems. The present paper is intended to give a brief summary of the crop production aspects of long-term trials.

Influence of NPK fertilization on weed flora in maize field

2014 ◽  
Vol 63 (1) ◽  
pp. 139-148 ◽  
Author(s):  
Éva Lehoczky ◽  
M. Kamuti ◽  
N. Mazsu ◽  
J. Tamás ◽  
D. Sáringer-Kenyeres ◽  
...  
Keyword(s):  
Plant Nutrition ◽  
Crop Production ◽  
N Availability ◽  
Weed Species ◽  
Negative Effects ◽  
Production Factors ◽  
Maize Field ◽  
Weed Flora ◽  
Npk Fertilization

Plant nutrition is one of the most important intensification factors of crop production. The utilization of nutrients, however, may be modified by a number of production factors, including weed presence. Thus, the knowledge of occurring weed species, their abundance, nutrient and water uptake is extremely important to establish an appropriate basis for the evaluation of their risks or negative effects on crops. That is why investigations were carried out in a long-term fertilization experiment on the influence of different nutrient supplies (Ø, PK, NK, NPK) on weed flora in maize field.The weed surveys recorded similar diversity on the experimental area: the species of A. artemisiifolia, S. halepense and D. stramonium were dominant, but C. album and C. hybridum were also common. These species and H. annuus were the most abundant weeds.Based on the totalized and average data of all treatments, density followed the same tendency in the experimental years. It was the highest in the PK treated and untreated plots, and significantly exceeded the values of NK fertilized areas. Presumably the better N availability promoted the development of nitrophilic weeds, while the mortality of other small species increased.Winter wheat and maize forecrops had no visible influence on the diversity and the intensity of weediness. On the contrary, there were consistent differences in the density of certain weed species in accordance to the applied nutrients. A. artemisiifolia was present in the largest number in the untreated control and PK fertilized plots. The density of S. halepense and H. annuus was also significantly higher in the control areas. The number of their individuals was smaller in those plots where N containing fertilizers were used. Contrary to them, the density of D. stramonium, C. album and C. hybridum was the highest in the NPK treatments.

Enhancement of Rural Income and Nutrition by Cultivating Pigeonpea Hybrids

2020 ◽  
Vol 7 (04) ◽  
Author(s):  
K B SAXENA ◽  
A K CHOUDHARY ◽  
RAFAT K SULTANA
Keyword(s):  
Cajanus Cajan ◽  
Family Income ◽  
National Level ◽  
Hybrid Breeding ◽  
High Food ◽  
Rural Income ◽  
Breeding Technology ◽  
On Farm ◽  
Food Value

Pigeonpea [Cajanus cajan (L.) Millsp.] is a favourite crop of rain-fed farmers due to its high food value, drought tolerance and various soil improving properties. The productivity enhancement of this crop has been a long-term goal at the national level but with a little success. In this context, the advent of hybrid breeding technology with over 30% on-farm yield advantages has provided a much-needed breakthrough. The hybrids in pigeonpea were bred using a stable CMS system and natural out-crossing. It is believed that the adoption of locally adapted hybrids would contribute significantly towards both family income and nutrition.

scholarly journals Cardiac Cell Therapy: Insights into the Mechanisms of Tissue Repair

2021 ◽  
Vol 22 (3) ◽  
pp. 1201
Author(s):  
Hsuan Peng ◽  
Kazuhiro Shindo ◽  
Renée R. Donahue ◽  
Ahmed Abdel-Latif
Keyword(s):  
Stem Cell ◽  
Immune Responses ◽  
Tissue Repair ◽  
Molecular Mechanisms ◽  
Clinical Evidence ◽  
Current Knowledge ◽  
Cell Delivery ◽  
Cardiac Cell Therapy ◽  
Stem Cell Treatment

Stem cell-based cardiac therapies have been extensively studied in recent years. However, the efficacy of cell delivery, engraftment, and differentiation post-transplant remain continuous challenges and represent opportunities to further refine our current strategies. Despite limited long-term cardiac retention, stem cell treatment leads to sustained cardiac benefit following myocardial infarction (MI). This review summarizes the current knowledge on stem cell based cardiac immunomodulation by highlighting the cellular and molecular mechanisms of different immune responses to mesenchymal stem cells (MSCs) and their secretory factors. This review also addresses the clinical evidence in the field.

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