Mechanism of oxidative stress in skeletal muscle atrophied by immobilization

To clarify the mechanism of oxidative stress in skeletal muscle atrophied by immobilization, we measured the activities of antioxidant enzymes and xanthine oxidase (XOD) and carried out the cytochemical study of hydrogen peroxide in a typical slow red muscle, the soleus. Male Wistar rats (15 wk old), of which ankle joints of one hindlimb were immobilized in the fully extended position, were killed after 4, 8, or 12 days. The activities of Mn-containing superoxide dismutase (Mn-SOD), Cu-Zn-containing superoxide dismutase (Cu-Zn-SOD), Se-dependent glutathione peroxidase (Se-GSHPx), glutathione S-transferase, catalase, and glutathione reductase were measured spectrophotometrically. The XOD activity and the concentrations of hypoxanthine, xanthine, and urate were measured using a high-performance liquid chromatography. The cytochemical study of hydrogen peroxide in short-term organ culture was performed using an electron microscope. Increased Cu-Zn-SOD and decreased Mn-SOD in atrophy might reflect increased generation of superoxide anions in the cytoplasm rather than in the mitochondria. The source of superoxide anions in the cytoplasm might be the increased superoxide-producing XOD. Enhanced generation of superoxide anions and increased Cu-Zn-SOD activity in atrophy suggested the enhanced generation of hydrogen peroxide in the cytoplasm. Due to the unchanged activity of Se-GSHPx and the unchanged or slightly increased activity of catalase in atrophy, the ability to degrade hydrogen peroxide might not increase so much. Hence, hydrogen peroxide is expected to be increased in atrophy. The cytochemical study supported this expectation.(ABSTRACT TRUNCATED AT 250 WORDS)

Download Full-text

Further investigation of the mechanism of Vitreoscilla hemoglobin (VHb) protection from oxidative stress in Escherichia coli

Biologia ◽

10.2478/s11756-011-0099-x ◽

2011 ◽

Vol 66 (5) ◽

Cited By ~ 2

Author(s):

Meltem Akbas ◽

Tugrul Doruk ◽

Serhat Ozdemir ◽

Benjamin Stark

Keyword(s):

Oxidative Stress ◽

Escherichia Coli ◽

Hydrogen Peroxide ◽

Superoxide Dismutase ◽

Stationary Phase ◽

Exponential Phase ◽

Vitreoscilla Hemoglobin ◽

Sod Activity ◽

E Coli ◽

Hydrogen Peroxide Treatment

AbstractIn Escherichia coli, Vitreoscilla hemoglobin (VHb) protects against oxidative stress, perhaps, in part, by oxidizing OxyR. Here this protection, specifically VHb-associated effects on superoxide dismutase (SOD) and catalase levels, was examined. Exponential or stationary phase cultures of SOD+ or SOD− E. coli strains with or without VHb and oxyR antisense were treated with 2 mM hydrogen peroxide without sublethal peroxide induction, and compared to untreated control cultures. The hydrogen peroxide treatment was toxic to both SOD+ and SOD− cells, but much more to SOD− cells; expression of VHb in SOD+ strains enhanced this toxicity. In contrast, the presence of VHb was generally associated in the SOD+ background with a modest increase in SOD activity that was not greatly affected by oxyR antisense or peroxide treatment. In both SOD+ and SOD− backgrounds, VHb was associated with higher catalase activity both in the presence and absence of peroxide. Contrary to its stimulatory effects in stationary phase, in exponential phase oxyR antisense generally decreased VHb levels.

Download Full-text

Effect of Nandrolone Treatment with And Without Resistance Training on Superoxide Dismutase Concentration and Pathology of Kidney Tissue in Rats

Journal of Clinical Research in Paramedical Sciences ◽

10.5812/jcrps.102497 ◽

2020 ◽

Vol In Press (In Press) ◽

Author(s):

Esfandiyar Heidari ◽

Seyed Ali Hosseini ◽

Mohammad Ali Azarbayjani

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Resistance Training ◽

Anabolic Steroids ◽

Kidney Tissue ◽

Sod Activity ◽

Significant Difference ◽

Stress Levels ◽

Sh Group ◽

Male Wistar Rats

Background: The prevalence of anabolic steroids abuse in athletes and non-athletes is associated with the risk of injury to various organs, but there are limited studies of oxidative changes in kidney tissue following nandrolone (N) administration. Objectives: the aim of this study was to investigate the effect of N treatment with and without resistance training (RT) on superoxide dismutase (SOD) concentration and tissue pathology of kidney tissue in rats. Methods: In this experimental study, 20 male Wistar rats were randomly divided into four groups of five rats including 1) control (C), 2) sham (normal saline) (Sh), 3) N, and 4) N + RT. Groups 3 and 4 received 10 mg/kg N peritoneally, and the N + RT group performed 1 m ladder climbing for eight weeks and three sessions per week. SOD levels of kidney tissue were measured by ELISA and radioimmunoassay. Hematoxylin-eosin (H&E) staining was used to evaluate oxidative stress levels in kidney tissue. One-way ANOVA with Bonferroni’s post- hoc tests were used for analysis of research findings in SPSS version 22 (P ≤ 0.05). Results: SOD levels in the C group were higher than the Sh (P = 0.001), N (P = 0.001), and N + RT (P = 0.001) groups. SOD levels were lower in the Sh group than in the N (P = 0.049) and N + RT (P = 0.001) groups. However, there was no significant difference in SOD levels in the N + RT group and N group (P = 0.28). Also, oxidative stress levels were normal in tissue studies in all groups. Conclusions: It seems that Ntreatment with and without RT reduces SOD activity in kidney tissue, but more studies are needed in this regard given the normality of tissue oxidative stress results.

Download Full-text

Superoxide dismutase mimetic drug tempol aggravates anti-GBM antibody-induced glomerulonephritis in mice

AJP Renal Physiology ◽

10.1152/ajprenal.00583.2009 ◽

2010 ◽

Vol 299 (2) ◽

pp. F445-F452 ◽

Cited By ~ 17

Author(s):

Hua Lu ◽

Junhui Zhen ◽

Tianfu Wu ◽

Ai Peng ◽

Ting Ye ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Superoxide Dismutase ◽

Basement Membrane ◽

Glomerular Basement Membrane ◽

Immunohistochemical Staining ◽

Treated Group ◽

First Line ◽

Sod Activity ◽

Leukocyte Influx

Oxidative stress plays an important role in the pathogenesis of anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM-GN). Superoxide dismutase (SOD) is the first line of defense against oxidative stress by converting superoxide to hydrogen peroxide (H2O2). We investigated the effect of the SOD mimetic drug tempol on anti-GBM-GN in mice. 129/svJ mice were challenged with rabbit anti-mouse-GBM sera to induce GN and subsequently divided into tempol (200 mg·kg−1·day−1, orally) and vehicle-treated groups. Routine histology, SOD and catalase activities, malondialdehyde (MDA), H2O2, and immunohistochemical staining for neutrophils, lymphocytes, macrophages, p65-NF-κB, and osteopontin were performed. Mice with anti-GBM-GN had significantly reduced renal SOD and catalase activities and increased H2O2 and MDA levels. Unexpectedly, tempol administration exacerbated anti-GBM-GN as evidenced by intensification of proteinuria, the presence of severe crescentic GN with leukocyte influx, and accelerated mortality in the treated group. Tempol treatment raised SOD activity and H2O2 level in urine, upregulated p65-NF-κB and osteopontin in the kidney, but had no effect on renal catalase activity. Thus tempol aggravates anti-GBM-GN by increasing production of H2O2 which is a potent NF-κB activator and as such can intensify inflammation and renal injury. This supposition is supported by increases seen in p65-NF-κB, osteopontin, and leukocyte influx in the kidneys of the tempol-treated group.

Download Full-text

Oxidative stress in rat liver during acute cadmium and ethanol intoxication

Journal of the Serbian Chemical Society ◽

10.2298/jsc110330174r ◽

2012 ◽

Vol 77 (2) ◽

pp. 159-176 ◽

Cited By ~ 17

Author(s):

Tatjana Radosavljevic ◽

Dusan Mladenovic ◽

Milica Ninkovic ◽

Danijela Vucevic ◽

Ivan Boricic ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Rat Liver ◽

Manganese Superoxide Dismutase ◽

Treated Group ◽

Sod Activity ◽

Pronounced Increase ◽

Orogastric Tube ◽

Liver Copper ◽

Male Wistar Rats

The aim of our study was to investigate the effects of binge drinking on prooxidant/antioxidant system in rat liver in acute cadmium (Cd) intoxication. In experiment male Wistar rats were used and divided into following groups: 1. control, 2. ethanol-treated group, in five subsequent doses of 2 g/kg administered by orogastric tube, 3. Cd-treated group in a single dose of 2.5 mg/kg intraperitoneally, 4. group that received Cd 12 hours after the last dose of ethanol. Blood and liver samples were collected for determination of oxidative stress parameters, 24 hours after treatment. When administered in combination, ethanol and Cd induced a more pronounced increase in serum and liver malondialdehyde level than either of these substances alone (p<0.01). Liver manganese superoxide dismutase (MnSOD) activity was increased both in ethanol and Cd-treated group (p<0.01), while liver copper/zinc superoxide dismutase (Cu/ZnSOD) activity was elevated in Cd group only. However, when administered in combination, ethanol and Cd induced a more pronounced decrease in liver MnSOD and Cu/ZnSOD activity 24 hours after treatment (p<0.01). Based on our study, it can be concluded that ethanol may act sinergistically with Cd in inducing lipid peroxidation and reduction in liver SOD activity.

Download Full-text

Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos

10.7287/peerj.preprints.3383 ◽

2017 ◽

Author(s):

Fadia Najjar ◽

Francine Rizk ◽

Gilles Carnac ◽

Rim Nassar ◽

Sara Jabak ◽

...

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Hydrogen Peroxide ◽

Superoxide Dismutase ◽

Protective Effect ◽

Zebrafish Embryos ◽

H Nmr ◽

Rhus Coriaria ◽

Etoac Fraction ◽

Superoxide Dismutase 2

Background and purpose Oxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect of R. coriaria sumac fruit extract on human myoblasts and zebrafish embryos in conditions of hydrogen peroxide-induced oxidative stress. Study design and Methods Crude ethanolic 70 % extract (CE) and its fractions was obtained from sumac fruits. The composition of sumac ethyl acetate EtOAc fraction was studied by 1H NMR. The viability of human myoblasts treated with CE and the EtOAc fraction was determined by trypan blue exclusion test. Oxidative stress, cell cycle and adhesion were analyzed by flow cytometry and microscopy. Gene expression was analyzed by qPCR. Results The EtOAc fraction (IC50 2.57 μg/mL) had the highest antioxidant activity and exhibited the best protective effect against hydrogen peroxide-induced oxidative stress. It also restored cell adhesion. This effect was mediated by superoxide dismutase 2 and catalase. Pre-treatment of zebrafish embryos with low concentrations of the EtOAc fraction protected them from hydrogen peroxide-induced death in vivo. 1H NMR analysis revealed the presence of gallic acid in this fraction. Conclusion Rhus coriaria extracts inhibited or slowed down the progress of skeletal muscle atrophy by decreasing oxidative stress via superoxide dismutase 2 and catalase-dependent mechanisms.

Download Full-text

Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos

10.7287/peerj.preprints.3383v1 ◽

2017 ◽

Author(s):

Fadia Najjar ◽

Francine Rizk ◽

Gilles Carnac ◽

Rim Nassar ◽

Sara Jabak ◽

...

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Hydrogen Peroxide ◽

Superoxide Dismutase ◽

Protective Effect ◽

Zebrafish Embryos ◽

H Nmr ◽

Rhus Coriaria ◽

Etoac Fraction ◽

Superoxide Dismutase 2

Download Full-text

Effects of Exercise on Oxidative Stress in Rats Induced by Ozone

The Scientific World JOURNAL ◽

10.1100/2012/135921 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 7

Author(s):

Catalina Martinez-Campos ◽

Eleazar Lara-Padilla ◽

Rosa Amalia Bobadilla-Lugo ◽

Robert David Kross ◽

Cleva Villanueva

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Aerobic Exercise ◽

Wistar Rats ◽

Acute Exercise ◽

Chronic Exercise ◽

Sod Activity ◽

Pilot Experiment ◽

Male Wistar Rats ◽

Adaptation To Exercise

Oxidative stress (OS) induced by acute exercise is reduced by chronic exercise. Ozone (O3) exposure produces OS. The aim of this study was to determine if aerobic exercise (AE) reduced OS produced by O3. A pilot experiment was performed with male Wistar rats submitted to AE (trained to swim 90 min/day). Adaptation to exercise was demonstrated three weeks after training by means of changes in reduced nitrates (NOx) in plasma. Therefore, two-week training was chosen for the following experiments. Six of twelve trained rats were exposed to O3(0.5 ppm, 4 h/day, one hour before exercise). Two groups of sedentary animals (n=6each) were used as controls, one of which was exposed to O3. At the end of the experiments NOx, 8-isoprostane (8-IP), malondialdehyde (MDA), superoxide dismutase (SOD) activity, and carbonyls (CBs) were measured in plasma. CBs did not change in any group. O3-induced OS was manifested by reduced NOxand SOD activity, as well as increased 8-IP and MDA. Exercise significantly blocked O3effects although SOD was also decreased by exercise (a greater drop occurring in the O3group). It is concluded that AE protects against OS produced by O3and the effect is independent of SOD.

Download Full-text

Glutathione peroxidase (EC 1.11.1.9), glutathione-S-transferase (EC 2.5.1.13), superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) activities in tissues of ducklings deprived of vitamin E and selenium

British Journal Of Nutrition ◽

10.1079/bjn19830111 ◽

1983 ◽

Vol 50 (2) ◽

pp. 437-444 ◽

Cited By ~ 16

Author(s):

Guang-Lu Xu ◽

Anthony T. Diplock

Keyword(s):

Skeletal Muscle ◽

Hydrogen Peroxide ◽

Superoxide Dismutase ◽

Vitamin E ◽

Glutathione Peroxidase ◽

Enzyme Activities ◽

Cumene Hydroperoxide ◽

The Other ◽

Glutathione S Transferase ◽

Different Tissues

1. Vitamin E and selenium deficiencies were produced in the Pekin duckling (Anser cinerens) and were characterized by the development of lesions after 14 d in gizzard, intestine, heart and skeletal muscle.2. The activities of glutathione peroxidase (EC 1.11.1.9), using hydrogen peroxide and cumene hydroperoxide as substrates, glutathione-S-transferase (EC 2.5.1.13), superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) were measured in homogenate supernatant fractions of liver, heart, intestine, gizzard and skeletal muscle of deficient ducklings and of control birds given vitamin E or Se or both.3. Glutathione peroxidase activities were dramatically lower in tissues of Se-deficient ducklings, and this was unaffected by vitamin E. No adaptive changes were seen in the activity of the other enzymes, even after 21 d when the deficiency was severe.4. It appeared likely that the variability of the enzyme activities, other than glutathione peroxidase, in the different tissues studied might explain differences among the tissues in susceptibility to peroxidative damage.

Download Full-text

Protective effect ofRhus coriariafruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos

PeerJ ◽

10.7717/peerj.4144 ◽

2017 ◽

Vol 5 ◽

pp. e4144 ◽

Cited By ~ 5

Author(s):

Fadia Najjar ◽

Francine Rizk ◽

Gilles Carnac ◽

Rim Nassar ◽

Sara Jabak ◽

...

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Hydrogen Peroxide ◽

Superoxide Dismutase ◽

Protective Effect ◽

Skeletal Muscle Atrophy ◽

Zebrafish Embryos ◽

H Nmr ◽

Etoac Fraction ◽

Superoxide Dismutase 2

Background and PurposeOxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect ofR. coriariasumac fruit extract on human myoblasts and zebrafish embryos in conditions of hydrogen peroxide-induced oxidative stress.Study Design and MethodsCrude ethanolic 70% extract (CE) and its fractions was obtained from sumac fruits. The composition of sumac ethyl acetate EtOAc fraction was studied by1H NMR. The viability of human myoblasts treated with CE and the EtOAc fraction was determined by trypan blue exclusion test. Oxidative stress, cell cycle and adhesion were analyzed by flow cytometry and microscopy. Gene expression was analyzed by qPCR.ResultsThe EtOAc fraction (IC502.57 µg/mL) had the highest antioxidant activity and exhibited the best protective effect against hydrogen peroxide-induced oxidative stress. It also restored cell adhesion. This effect was mediated by superoxide dismutase 2 and catalase. Pre-treatment of zebrafish embryos with low concentrations of the EtOAc fraction protected them from hydrogen peroxide-induced deathin vivo.1H NMR analysis revealed the presence of gallic acid in this fraction.ConclusionRhus coriariaextracts inhibited or slowed down the progress of skeletal muscle atrophy by decreasing oxidative stress via superoxide dismutase 2 and catalase-dependent mechanisms.

Download Full-text

10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0042 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Kiptiyah Kiptiyah ◽

Widodo Widodo ◽

Gatot Ciptadi ◽

Aulanni’am Aulanni’Am ◽

Mohammad A. Widodo ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Culture ◽

Superoxide Dismutase ◽

In Silico ◽

Cumulus Cell ◽

Cumulus Cells ◽

Sod Activity ◽

In Silico Studies ◽

Incubation Periods

AbstractBackgroundWe investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells.MethodsFor the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.ResultsThe in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10˗gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.Conclusions10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.

Download Full-text