scholarly journals Effects of Exercise on Oxidative Stress in Rats Induced by Ozone

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Catalina Martinez-Campos ◽  
Eleazar Lara-Padilla ◽  
Rosa Amalia Bobadilla-Lugo ◽  
Robert David Kross ◽  
Cleva Villanueva
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Aerobic Exercise ◽  
Wistar Rats ◽  
Acute Exercise ◽  
Chronic Exercise ◽  
Sod Activity ◽  
Pilot Experiment ◽  
Male Wistar Rats ◽  
Adaptation To Exercise

Oxidative stress (OS) induced by acute exercise is reduced by chronic exercise. Ozone (O3) exposure produces OS. The aim of this study was to determine if aerobic exercise (AE) reduced OS produced by O3. A pilot experiment was performed with male Wistar rats submitted to AE (trained to swim 90 min/day). Adaptation to exercise was demonstrated three weeks after training by means of changes in reduced nitrates (NOx) in plasma. Therefore, two-week training was chosen for the following experiments. Six of twelve trained rats were exposed to O3(0.5 ppm, 4 h/day, one hour before exercise). Two groups of sedentary animals (n=6each) were used as controls, one of which was exposed to O3. At the end of the experiments NOx, 8-isoprostane (8-IP), malondialdehyde (MDA), superoxide dismutase (SOD) activity, and carbonyls (CBs) were measured in plasma. CBs did not change in any group. O3-induced OS was manifested by reduced NOxand SOD activity, as well as increased 8-IP and MDA. Exercise significantly blocked O3effects although SOD was also decreased by exercise (a greater drop occurring in the O3group). It is concluded that AE protects against OS produced by O3and the effect is independent of SOD.

scholarly journals The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

2014 ◽  
Vol 66 (3) ◽  
pp. 1075-1081
Author(s):  
Ivan Simic ◽  
Violeta Iric-Cupic ◽  
Rada Vucic ◽  
Marina Petrovic ◽  
Violeta Mladenovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Wistar Rats ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

scholarly journals Effect of Nandrolone Treatment with And Without Resistance Training on Superoxide Dismutase Concentration and Pathology of Kidney Tissue in Rats

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Esfandiyar Heidari ◽  
Seyed Ali Hosseini ◽  
Mohammad Ali Azarbayjani
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Resistance Training ◽  
Anabolic Steroids ◽  
Kidney Tissue ◽  
Sod Activity ◽  
Significant Difference ◽  
Stress Levels ◽  
Sh Group ◽  
Male Wistar Rats

Background: The prevalence of anabolic steroids abuse in athletes and non-athletes is associated with the risk of injury to various organs, but there are limited studies of oxidative changes in kidney tissue following nandrolone (N) administration. Objectives: the aim of this study was to investigate the effect of N treatment with and without resistance training (RT) on superoxide dismutase (SOD) concentration and tissue pathology of kidney tissue in rats. Methods: In this experimental study, 20 male Wistar rats were randomly divided into four groups of five rats including 1) control (C), 2) sham (normal saline) (Sh), 3) N, and 4) N + RT. Groups 3 and 4 received 10 mg/kg N peritoneally, and the N + RT group performed 1 m ladder climbing for eight weeks and three sessions per week. SOD levels of kidney tissue were measured by ELISA and radioimmunoassay. Hematoxylin-eosin (H&E) staining was used to evaluate oxidative stress levels in kidney tissue. One-way ANOVA with Bonferroni’s post- hoc tests were used for analysis of research findings in SPSS version 22 (P ≤ 0.05). Results: SOD levels in the C group were higher than the Sh (P = 0.001), N (P = 0.001), and N + RT (P = 0.001) groups. SOD levels were lower in the Sh group than in the N (P = 0.049) and N + RT (P = 0.001) groups. However, there was no significant difference in SOD levels in the N + RT group and N group (P = 0.28). Also, oxidative stress levels were normal in tissue studies in all groups. Conclusions: It seems that Ntreatment with and without RT reduces SOD activity in kidney tissue, but more studies are needed in this regard given the normality of tissue oxidative stress results.

Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Sunny O. Abarikwu ◽  
Sussan Benjamin ◽  
Sunday Godspower Ebah ◽  
Godbless Obilor ◽  
Goodluck Agbam
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Protective Effect ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Negative Effects ◽  
Male Wistar Rats ◽  
Moringa Oleifera Oil

Abstract:Background:Various parts of theMethods:Male Wistar rats were administered MO (1.798 mg/kg p.o.) or HgClResults:In the liver, malondialdehyde (MDA) level, glutathione (GSH), and superoxide dismutase (SOD) activities were higher whereas catalase (CAT) activity was lower in the HgClConclusions:MO decreased the negative effects of Hg-induced oxidative stress in rats.

scholarly journals Oxidative stress in rat liver during acute cadmium and ethanol intoxication

2012 ◽  
Vol 77 (2) ◽  
pp. 159-176 ◽  
Author(s):  
Tatjana Radosavljevic ◽  
Dusan Mladenovic ◽  
Milica Ninkovic ◽  
Danijela Vucevic ◽  
Ivan Boricic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Rat Liver ◽  
Manganese Superoxide Dismutase ◽  
Treated Group ◽  
Sod Activity ◽  
Pronounced Increase ◽  
Orogastric Tube ◽  
Liver Copper ◽  
Male Wistar Rats

The aim of our study was to investigate the effects of binge drinking on prooxidant/antioxidant system in rat liver in acute cadmium (Cd) intoxication. In experiment male Wistar rats were used and divided into following groups: 1. control, 2. ethanol-treated group, in five subsequent doses of 2 g/kg administered by orogastric tube, 3. Cd-treated group in a single dose of 2.5 mg/kg intraperitoneally, 4. group that received Cd 12 hours after the last dose of ethanol. Blood and liver samples were collected for determination of oxidative stress parameters, 24 hours after treatment. When administered in combination, ethanol and Cd induced a more pronounced increase in serum and liver malondialdehyde level than either of these substances alone (p<0.01). Liver manganese superoxide dismutase (MnSOD) activity was increased both in ethanol and Cd-treated group (p<0.01), while liver copper/zinc superoxide dismutase (Cu/ZnSOD) activity was elevated in Cd group only. However, when administered in combination, ethanol and Cd induced a more pronounced decrease in liver MnSOD and Cu/ZnSOD activity 24 hours after treatment (p<0.01). Based on our study, it can be concluded that ethanol may act sinergistically with Cd in inducing lipid peroxidation and reduction in liver SOD activity.

scholarly journals THE EFFECTS OF EXPOSURE DURATION TO ELECTRONIC CIGARETTE SMOKE ON DIFFERENCES IN SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE IN BLOOD OF WISTAR RATS

2019 ◽  
pp. 13-16
Author(s):  
RIVAN VIRLANDO SURYADINATA ◽  
BAMBANG WIRJATMADI ◽  
MERRYANA ADRIANI ◽  
SRI SUMARMI
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Cell Damage ◽  
The Body ◽  
Electronic Cigarette ◽  
Male Wistar Rats ◽  
The Relationship

Objective: Exposure to electronic cigarette smoke causes an increase of free radicals. Physiologically, the body produces antioxidant superoxide dismutase to neutralize the free radicals. However, an excessive increase of the free radicals will result in an imbalance between the amount of free radicals and antioxidants. The free radicals in the body can trigger lipid peroxidation so that it will result in oxidative stress causing cell damage. Malondialdehyde increase is a marker of oxidative stress in the body. The aim of this research is to understand the changes and the relationship between levels of superoxide dismutase and malondialdehyde in the blood due to exposure to electronic cigarette smoke. Methods: This research is an experimental study using male Wistar rats as experimental animal models. In the study, the exposure to electronic cigarette smoke with different duration of administration was carried out. Next, blood samples were taken to check the levels of superoxide dismutase and malondialdehyde. Results: The results showed a difference in antioxidant levels between antioxidant Superoxide Dismutase and malondialdehyde (p<0.05). Meanwhile the relationship between the two groups showed a strong (r = 0.893) and significant (p = 0.000) relationship. Conclusion: The exposure to electronic cigarette smoke can reduce the level of antioxidant superoxide dismutase and increase the level of malondialdehyde in blood. In addition, changes in the levels of antioxidant superoxide dismutase and malondialdehyde had a strong and significant relationship.

scholarly journals Constant darkness conditions modulate the effects of melatonin and luzindole on the antioxidant enzyme activities and levels of retinol and α-tocopherol in rats

2019 ◽  
Vol 64 (3) ◽  
Author(s):  
Svetlana Kalinina ◽  
Viktor Ilyukha ◽  
Evgeniy Khizhkin ◽  
Irina Baishnikova ◽  
Ekaterina Antonova ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Wistar Rats ◽  
Constant Darkness ◽  
Antioxidant Enzyme Activities ◽  
Light Conditions ◽  
Melatonin Receptor ◽  
Sod Activity ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Normal Light

This study was conducted to evaluate the effects of both exogenous melatonin and melatonin receptor antagonist luzindole on the activities of antioxidant enzymes (AOE) (superoxide dismutase, SOD; catalase, CAT) and the level of low-molecular antioxidant vitamins (retinol, α-tocopherol) in male Wistar rats kept in normal light conditions (LD 12:12) or constant darkness (DD). In LD, while melatonin had no influence on the studied antioxidants, luzindole caused an increase in retinol and a decrease in α-tocopherol contents in the liver compared to the control. In DD, with no influence on AOE activities, both drugs exerted similar effects on the liver retinol and kidney α-tocopherol contents, increasing them in comparison with control. Exposing the animals to DD induced an increase in kidney SOD activity and in liver retinol content. Moreover, DD-mel rats had higher SOD activity in the liver and kidney and a higher retinol level in the liver compared to LD-mel ones; DD-luz rats had a higher liver retinol content compared to LD-luz ones. Liver retinol level seems to be the most sensitive to influence of DD, melatonin and luzindole; the data are probably connected with the involvement of vitamin A in the regulation of circadian rhythms.

scholarly journals The Effect of Uncaria gambir Roxb. Extract on Superoxide Dismutase Activity in Proteinuric Wistar Rats Model

2021 ◽  
Vol 9 (B) ◽  
pp. 1505-1509
Author(s):  
Rosmayanti Syafriani Siregar ◽  
Aznan Lelo ◽  
Delyuzar Harris ◽  
Oke Rina Ramayani ◽  
Muhammad Ichwan
Keyword(s):  
Superoxide Dismutase ◽  
Wistar Rats ◽  
Control Group ◽  
Blood Collection ◽  
Creatinine Ratio ◽  
Urine Protein ◽  
Puromycin Aminonucleoside ◽  
Sod Activity ◽  
Control Group Design ◽  
Male Wistar Rats

Nephrotic syndrome (NS) is a glomerular disease that is most often found in children with proteinuric as clinical manifestation. Gambier extract is a traditional medicine that has antioxidant effect. Its use to treat  proteinuric has never been done. Therefore, we conducted an analytical study on the effect of gambier on proteinuric and superoxide dismutase (SOD) activity in proteinuric Wistar rat’s model. This research is an experimental study with a posttest control group design. The aim is analyzing the effect of giving gambier extract in decreasing urinary protein creatinine ratio and increasing SOD activity. Male Wistar rats were given an injection of puromycin aminonucleoside 1.5mg/100g body weight (BW), subcutaneously for 5 consecutive days for being nephrosis. The experimental animals in this study were divided into four groups: K1, the control group, consist of group of rats that were injected with 0.15ml/100 g BW of aquabidest and not given gambier extract. While K2 group consist of rats induced by Puromycin and given 1 ml of aquabidest. K3 consist of groups of rats induced by Puromycin and then given gambier extract 26mg/200g BW. Group K4 consist of rats induced by Puromycin and then given 80mg/200g BW of gambier extract. The four groups were given treatment for 14 days, after which the rats were placed in a metabolic cage for 24 hours to collect urine samples and then knocked down with ketamine for intra-cardiac blood collection. The results of this study showed differences between the four groups of rats in terms of the mean urine protein/creatinine ratio (p=0.015) and SOD activity (p=0.036). Groups of rats that were given gambier extracts 80mg/200g BW had lower urine protein / creatinine ratio and higher SOD activity. Therapy of gambier 80 mg/200g BW is better in managing proteinuric compared to 26mg/200g.

Mechanism of oxidative stress in skeletal muscle atrophied by immobilization

1993 ◽  
Vol 265 (6) ◽  
pp. E839-E844 ◽  
Author(s):  
H. Kondo ◽  
I. Nakagaki ◽  
S. Sasaki ◽  
S. Hori ◽  
Y. Itokawa
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
High Performance ◽  
Superoxide Anions ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Cytochemical Study ◽  
Male Wistar Rats

To clarify the mechanism of oxidative stress in skeletal muscle atrophied by immobilization, we measured the activities of antioxidant enzymes and xanthine oxidase (XOD) and carried out the cytochemical study of hydrogen peroxide in a typical slow red muscle, the soleus. Male Wistar rats (15 wk old), of which ankle joints of one hindlimb were immobilized in the fully extended position, were killed after 4, 8, or 12 days. The activities of Mn-containing superoxide dismutase (Mn-SOD), Cu-Zn-containing superoxide dismutase (Cu-Zn-SOD), Se-dependent glutathione peroxidase (Se-GSHPx), glutathione S-transferase, catalase, and glutathione reductase were measured spectrophotometrically. The XOD activity and the concentrations of hypoxanthine, xanthine, and urate were measured using a high-performance liquid chromatography. The cytochemical study of hydrogen peroxide in short-term organ culture was performed using an electron microscope. Increased Cu-Zn-SOD and decreased Mn-SOD in atrophy might reflect increased generation of superoxide anions in the cytoplasm rather than in the mitochondria. The source of superoxide anions in the cytoplasm might be the increased superoxide-producing XOD. Enhanced generation of superoxide anions and increased Cu-Zn-SOD activity in atrophy suggested the enhanced generation of hydrogen peroxide in the cytoplasm. Due to the unchanged activity of Se-GSHPx and the unchanged or slightly increased activity of catalase in atrophy, the ability to degrade hydrogen peroxide might not increase so much. Hence, hydrogen peroxide is expected to be increased in atrophy. The cytochemical study supported this expectation.(ABSTRACT TRUNCATED AT 250 WORDS)

Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Taye J. Lasisi ◽  
Shehu-Tijani T. Shittu ◽  
Jude I. Abeje ◽  
Kehinde J. Ogunremi ◽  
Seyyid A. Shittu
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Wistar Rats ◽  
Paradoxical Sleep ◽  
Salivary Secretion ◽  
Control Group ◽  
Paradoxical Sleep Deprivation ◽  
Sod Activity ◽  
Submandibular Glands ◽  
Male Wistar Rats

Abstract Objectives Paradoxical sleep deprivation has been associated with impaired salivary secretion in rats. However, the mechanism that underlies this is not known. Therefore, this study assessed salivary and serum oxidative stress levels following paradoxical sleep deprivation in rats. Methods Twenty-one male Wistar rats randomly divided into three groups of seven rats each as; Control (C); partial sleep-deprived (PSD); and total sleep-deprived (TSD) were used. Malondialdehyde (MDA) concentration, Superoxide dismutase (SOD), and catalase activities were evaluated in saliva, serum, and submandibular glands after seven days of sleep deprivation. Data were expressed as mean ± standard error of the mean and analyzed using one-way ANOVA, Tukey HSD post hoc, and Pearson’s correlation tests. Results Serum MDA levels were significantly higher in both the TSD and PSD groups compared to the control group whereas only the TSD group showed higher submandibular MDA levels compared to the PSD group and the control group. Submandibular SOD activity was significantly lower in both the TSD and PSD groups compared to the control group. Serum catalase activity was significantly lower in the TSD group only compared to the control group. Conclusions These results have demonstrated for the first time that paradoxical sleep deprivation was associated with changes in the oxidant/antioxidant defense system in the submandibular salivary glands of male Wistar rats which may contribute to impairment in salivary secretion.

scholarly journals Useful Effects of Curcumin Supplementation on Gastric Superoxide Dismutase Activity and Serum Malondialdehyde Level During Endurance Training in Male Wistar Rats

2020 ◽  
Vol 22 (2) ◽  
Author(s):  
Ali Gorzi ◽  
Mozhgan Asadi
Keyword(s):  
Superoxide Dismutase ◽  
Endurance Training ◽  
Wistar Rats ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Antioxidant Defenses ◽  
Gastric Ulcers ◽  
Gastric Tissue ◽  
Sod Activity ◽  
Male Wistar Rats

Background: Strenuous endurance training compromises the antioxidant defenses of the body and attempts to find supplements to heal gastric ulcers induced by strenuous endurance training are increasing. Objectives: Therefore, the current study aimed at investigating the effects of curcumin supplementation during nine weeks of endurance training on gastric antioxidant capacity (superoxide dismutase; SOD) and lipid peroxidation (malondialdehyde; MDA) of serum in male Wistar rats. Methods: The current applied research was conducted on 26 male Wistar rats aged nine weeks and weighed 215.87 ± 20.49 g. After one week of acclimation to training, the rats were randomly assigned to four groups: the control, curcumin, endurance, and endurance + curcumin. During the nine weeks (five sessions per week), the rats performed an incremental endurance training (up to 70 minutes at 35 m/minute), and the curcumin supplement at 30 mg/kg of body weight was injected intraperitoneally three times per week. SOD activity was assayed by the spectrophotometric method, and serum MDA level was measured utilizing the thiobarbituric acid reactive substances. For data analysis, ANOVA was employed using SPSS version 24. Results: The results of the study revealed that strenuous endurance training significantly reduced the activity of the gastric SOD enzyme (P < 0.05) and increased serum MDA level (P < 0.05), while curcumin supplementation increased gastric SOD enzyme activity, decreased serum MDA level, and resulted in the restoration of antioxidant defenses. Conclusions: Prolonged strenuous endurance training induces oxidative stress to gastric tissue, and curcumin supplementation restores the antioxidant defense of the gastric tissue and body.

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