Effects of estrogen on whole animal and tissue glucose use in female and male rainbow trout

1992 ◽  
Vol 263 (6) ◽  
pp. R1241-R1247 ◽  
Author(s):  
B. S. Washburn ◽  
M. L. Bruss ◽  
E. H. Avery ◽  
R. A. Freedland

Reports of changes in carbohydrate metabolism during vitellogenesis in fish prompted an investigation of the effects of estrogen on glucose utilization in rainbow trout. Estrogen pellets were implanted in both female and male fish, and a third group of male fish was given a sham operation. After cannulation of the dorsal aorta, D-[1-3H]glucose and 2-deoxy-D-[U-14C]glucose were injected into the fish to observe whole animal and tissue glucose use. We found that estrogen does not affect glucose turnover rate or transit time but causes a decrease in plasma glucose concentration and size of the glucose mixing pool. Adipose tissue in female fish utilized glucose at a higher rate than sham fish. Ovarian tissue used more glucose per kilogram of body weight than the testes of the male fish. Regardless of treatment, brain had the highest rate of glucose consumption per gram of tissue, followed by gonads and red blood cells. Muscle and adipose tissue utilized only small amounts (< 1 nmol.g tissue-1.min-1) of glucose. We conclude that an increase in the rate of whole body glucose use is not responsible for the fall in plasma glucose caused by estrogen and seen during vitellogenesis.

1972 ◽  
Vol 50 (9) ◽  
pp. 1183-1188 ◽  
Author(s):  
M. Chen ◽  
G. Power

In samples taken monthly throughout the year the percentage of American smelt in Lake Ontario and Lake Erie containing cysts of Glugea hertwigi was 5.2% and 62.7% respectively. Sexual differences in incidence were observed, the significance of which was uncertain as results from the two lakes were contradictory.In male fish infection was almost entirely restricted to the digestive tract with few cysts in the liver, skin, and testes. In female fish the digestive tract and ovaries were similarly infected.Seasonal fluctuations in Glugea infection were obvious and seemed correlated with the gonadal cycle. In both sexes the highest parasite load corresponded with the onset of maturation.A striking difference in fecundity between the two smelt populations was attributed to the Glugea infection. In females parasite cysts replaced ovarian tissue, causing a reduction in the number of maturing eggs.


2004 ◽  
Vol 287 (6) ◽  
pp. E1195-E1201 ◽  
Author(s):  
Theodore W. Zderic ◽  
Simon Schenk ◽  
Christopher J. Davidson ◽  
Lauri O. Byerley ◽  
Edward F. Coyle

We have recently reported that, during moderate intensity exercise, low muscle glycogen concentration and utilization caused by a high-fat diet is associated with a marked increase in fat oxidation with no effect on plasma glucose uptake (Rd glucose). It is our hypothesis that this increase in fat oxidation compensates for low muscle glycogen, thus preventing an increase in Rd glucose. Therefore, the purpose of this study was to determine whether low muscle glycogen availability increases Rd glucose under conditions of impaired fat oxidation. Six cyclists exercised at 50% peak O2 consumption (V̇o2 peak) for 1 h after 2 days on either a high-fat (HF, 60% fat, 24% carbohydrate) or control (CON, 22% fat, 65% carbohydrate) diet to manipulate muscle glycogen to low and normal levels, respectively. Two hours before the start of exercise, subjects ingested 80 mg of propanolol (βB), a nonselective β-adrenergic receptor blocker, to impair fat oxidation during exercise. HF significantly decreased calculated muscle glycogen oxidation ( P < 0.05), and this decrease was partly compensated for by an increase in fat oxidation ( P < 0.05), accompanied by an increase in whole body lipolysis ( P < 0.05), despite the presence of βB. Although HF increased fat oxidation, plasma glucose appearance rate, Rd glucose, and glucose clearance rate were also significantly increased by 13, 15, and 26%, respectively (all P < 0.05). In conclusion, when lipolysis and fat oxidation are impaired, in this case by βB, fat oxidation cannot completely compensate for a reduction in muscle glycogen utilization, and consequently plasma glucose turnover increases. These findings suggest that there is a hierarchy of substrate compensation for reduced muscle glycogen availability after a high-fat, low-carbohydrate diet, with fat being the primary and plasma glucose the secondary compensatory substrate. This apparent hierarchy likely serves to protect against hypoglycemia when endogenous glucose availability is low.


2017 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
Otong Zenal Arifin ◽  
Wahyulia Cahyanti ◽  
Jojo Subagja ◽  
Anang Hari Kristanto

Ikan tambakan berpotensi dibudidayakan karena memiliki keunggulan seperti kemampuan beradaptasi terhadap perairan dengan kadar oksigen terlarut rendah dan tergolong ikan dengan nilai fekunditas yang tinggi. Penelitian untuk mengetahui keragaan fenotipe ikan tambakan hasil domestikasi telah dilakukan di Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Bogor. Tujuan dari penelitian ini adalah untuk mengkarakterisasi bentuk morfologi berdasarkan morfometrik, meristik, dan warna yang berguna dalam pengelolaan pembenihan dan budidaya ikan tambakan. Pengambilan data dilakukan melalui pengamatan bentuk tubuh dan genitalia ikan jantan dan betina, pengukuran bagian tubuh, penghitungan jumlah dan jenis jari sirip, linea lateralis, warna ikan dan morfometrik. Hasil yang diperoleh menunjukkan bentuk tubuh ikan jantan lebih ramping dibanding ikan betina, ikan betina mempunyai rasio panjang standar terhadap tinggi badan sebesar 2,08±0,117; ikan jantan sebesar 2,26±0,095. Rasio panjang standar terhadap lebar badan pada ikan jantan adalah 0,95±0,018 dan ikan betina 1,01±0,025. Nilai koefisien variasi (CV) rerata seluruh karakter tubuh tergolong rendah, dengan nilai 12,2±10,73. Karakter C4 (awal sirip dorsal-akhir sirip anal) merupakan karakter nilai CV paling rendah yaitu 3,2% dan karakter D1 (akhir sirip anal-awal sirip ekor bawah) mempunyai nilai CV tertinggi yaitu 43,8%. Berdasarkan karakter meristik dan warna, tidak terdapat perbedaan antara jantan dan betina. Warna ikan tambakan terdiri atas warna punggung hijau keabuan (TC 613), warna operculum hijau keperakan (TC 613), warna perut perak sampai keabuan (TC 521) dan warna gonad kuning oranye (TC 023).Kissing gouramy has potentially to be cultivated due to the ability to adapt on swampy waters and has high eggs fecundity. Research on phenotype performance of domesticated kissing gouramy was done at the Institute for Freshwater Aquaculture Research and Development, Bogor. The purpose of this study was to characterize morphological forms based on the morphometrics, meristics, and color of domesticated fish that will be useful in the aquaculture management. The data were collected through observation of body shape and genitalia of male and female fish, measurement of body parts, counting the number and the type of fin, linea lateralis, fish color and the morphometric measurement. The obtained results showed that the body shape of the male fish was slender than that of the female fish, the female fish had a ratio of standard length to the height of 2.08 ± 0.117, male fish of 2.26 ± 0.095. The standard length ratio to body width in male fish was 0.95 ± 0.018 and female fish was 1.01 ± 0.025. The mean value of coefficient variation (CV) of the whole body character was low, with value 12,2 ± 10,73. C4 character (beginning of dorsal fin-end of anal fin) was the lowest character of CV value of 3.2% and D1 character (final anal fin-bottom caudal fin) had the highest CV value of 43.8%. Based on the meristic and color character there was no difference between male and female. The dorsal, overculum, ventral part and matured gonad of domesticated kissing gouramy fish had gray-green colour (TC 613), silver-green colour (TC 613), silver-gray to silver colour (TC 521) and orange yellow colour (TC 023) respectively.


1995 ◽  
Vol 78 (4) ◽  
pp. 1296-1302 ◽  
Author(s):  
A. L. Vallerand ◽  
J. Zamecnik ◽  
I. Jacobs

To clarify the source of increased carbohydrate oxidation during cold stress, six males rested for 3 h at 29 and 10 degrees C dressed only in shorts. After priming the blood glucose and bicarbonate pools, [U-13C6]glucose was infused for 3 h in each condition to determine the plasma glucose rate of appearance (Ra) or turnover under relative steady-state conditions. Plasma enrichment (mol %excess) was determined by selective ion-monitoring gas chromatography-mass spectrometry. Cold exposure decreased rectal temperature and mean skin temperature and increased heat debt, metabolic rate, and whole body lipid and carbohydrate oxidation (CHOox) compared with the same subjects at thermal neutrality (P < 0.05). Cold exposure significantly increased Ra from 13.18 +/- 0.70 to 16.22 +/- 0.43 mumol.kg-1.min-1 (P < 0.05). Plasma glucose clearance was elevated commensurately by the cold (from 2.68 +/- 0.16 to 3.55 +/- 0.14 ml.kg-1.min-1; P < 0.05). If we assume that Ra is completely oxidized (thus equivalent to maximum rates of plasma glucose oxidation) [J. A. Romijn, E. F. Coyle, L. S. Sidossis, A. Gastaldelli, J. F. Horowitz, E. Endert, and R. R. Wolfe. Am. J. Physiol. 265 (Endocrinol. Metab. 28): E380-E391, 1993], the minimum rates of glycogen and lactate oxidation in the cold would be the difference between CHOox and glucose oxidation (approximately 14.0 +/- 3.0 mumol.kg-1.min-1). Therefore, under the present laboratory conditions, 54% of CHOox would be fueled by plasma glucose oxidation, whereas the remaining 46% would be derived from the combination of glycogen and lactate oxidation.(ABSTRACT TRUNCATED AT 250 WORDS)


1994 ◽  
Vol 267 (5) ◽  
pp. R1226-R1234 ◽  
Author(s):  
T. G. West ◽  
C. J. Brauner ◽  
P. W. Hochachka

The involvement of circulatory glucose in the energy provision of skeletal muscle and heart of swimming carp was examined. Plasma glucose concentration varied from 3 to 17 mM among individual carp, and estimates of glucose turnover rate (RT) were positively correlated with plasma glucose level in resting fish (range 1.6-6.3 mumol.min-1.kg-1) and in swimming fish (range 4.2-10.7 mumol.min-1.kg-1). Carp that were exercised at 80% of their critical swimming speed displayed a twofold higher RT at any given plasma glucose concentration. Metabolic clearance rate also doubled in swimming carp (1.0 +/- 0.1 ml.min-1.kg-1) relative to resting controls (0.5 +/- 0.1 ml.min-1.kg-1). Indexes of muscle glucose utilization (GUI), determined with 2-deoxy-D-[14C]glucose, indicated that glucose utilization in red muscle was not dependent on plasma glucose concentration; however, glucose utilization in this muscle mass was threefold higher in swimming fish than in resting control fish. On the basis of whole body aerobic scope measurements in carp, it was estimated that circulatory glucose potentially comprised 25-30% of the total fuel oxidation in the active red muscle mass. GUI in heart was positively correlated with plasma glucose concentration, and it is possible that glucose availability had considerable influence on the pattern of myocardial substrate oxidation in resting and active carp. Carp are somewhat more reliant than rainbow trout on glucose for locomotor energetics, correlating with species differences in swimming capability and with the greater capacity of omnivorous carp to tolerate dietary glucose.


1968 ◽  
Vol 46 (4) ◽  
pp. 797-803 ◽  
Author(s):  
W. C. Mackay ◽  
D. D. Beatty

The level of plasma glucose was investigated in specimens of white sucker and northern pike which had been caught at different times of the year by both gill net and trap net. Blood samples were collected for analysis within 1 minute of the time the fish were first handled.Male white suckers undergo a significant seasonal change in plasma glucose levels but female suckers do not. The plasma glucose of the female fish of both species was more variable than that of male fish sampled at the same time. At certain times of the year male fish of both species had a significantly lower plasma glucose concentration than female fish. Fish which had been caught by gill net or held in the laboratory were found to have higher, more variable plasma glucose levels than fish which were sampled from the trap net.


2016 ◽  
Vol 113 (10) ◽  
pp. 2708-2713 ◽  
Author(s):  
Xiaoxue Yuan ◽  
Tao Hu ◽  
Han Zhao ◽  
Yuanyuan Huang ◽  
Rongcai Ye ◽  
...  

Polycystic ovary syndrome (PCOS), which is characterized by anovulation, hyperandrogenism, and polycystic ovaries, is a complex endocrinopathy. Because the cause of PCOS at the molecular level is largely unknown, there is no cure or specific treatment for PCOS. Here, we show that transplantation of brown adipose tissue (BAT) reversed anovulation, hyperandrogenism, and polycystic ovaries in a dehydroepiandrosterone (DHEA)-induced PCOS rat. BAT transplantation into a PCOS rat significantly stabilized menstrual irregularity and improved systemic insulin sensitivity up to a normal level, which was not shown in a sham-operated or muscle-transplanted PCOS rat. Moreover, BAT transplantation, not sham operation or muscle transplantation, surprisingly improved fertility in PCOS rats. Interestingly, BAT transplantation activated endogenous BAT and thereby increased the circulating level of adiponectin, which plays a prominent role in whole-body energy metabolism and ovarian physiology. Consistent with BAT transplantation, administration of adiponectin protein dramatically rescued DHEA-induced PCOS phenotypes. These results highlight that endogenous BAT activity is closely related to the development of PCOS phenotypes and that BAT activation might be a promising therapeutic option for the treatment of PCOS.


1997 ◽  
Vol 272 (6) ◽  
pp. G1530-G1539 ◽  
Author(s):  
C. Cherbuy ◽  
B. Darcy-Vrillon ◽  
L. Posho ◽  
P. Vaugelade ◽  
M. T. Morel ◽  
...  

We have reported previously that a high glycolytic capacity develops soon after birth in enterocytes isolated from suckling newborn pigs. In the present work, we investigated whether such metabolic changes could affect intestinal glucose utilization in vivo and examined possible variations in glucose metabolism along the small intestine. Glucose utilization by individual tissues was assessed using the 2-deoxyglucose technique. The overall glucose utilization rate was doubled in suckling vs. fasting 2-day-old pigs because of significantly higher rates in all tissues studied, except for the brain. In parallel, enterocytes were isolated from the proximal, medium, or distal jejunoileum of newborn vs. 2-day-old pigs and assessed for their capacity to utilize, transport, and phosphorylate glucose. Intestinal glucose consumption accounted for approximately 15% of glucose turnover rate in suckling vs. 8% in fasting pigs. Moreover, there was a proximal-to-distal gradient of glucose utilization in the intestinal mucosa of suckling pigs. Such a gradient was also evidenced on isolated enterocytes. The stimulation of both hexokinase activity (HK2 isoform) and basolateral glucose transporter (GLUT2), as observed in the proximal jejunum, could account for such a site-specific effect of suckling.


Author(s):  
Ruyu Liu ◽  
Caitlyn G Edwards ◽  
Corinne N Cannavale ◽  
Isabel R Flemming ◽  
Morgan R Chojnacki ◽  
...  

Abstract Background Breastfeeding is associated with healthier weight and nutrient status in early life. However, the impact of breastfeeding on carotenoid status beyond infancy, and the influence of adiposity, is unknown. Objective The aim of the study was to retrospectively investigate the relationship between breastfeeding and carotenoid status, and the mediating effect of weight status and adiposity on this relationship among school-aged children. Methods This was a secondary analysis of baseline data collected from a randomized-controlled clinical trial. (ClinicalTrials.gov Identifier: NCT03521349). 7–12-year-old (n = 81) children were recruited from East-Central Illinois. Dual-energy x-ray absorptiometry (DXA) was used to assess visceral adipose tissue (VAT) and whole-body adiposity (%Fat). Weight was obtained to calculated body mass index percentile (BMI %ile). Skin carotenoids were assessed via reflection spectroscopy. Macular carotenoids were assessed as macular pigment optical density (MPOD). Dietary, birth, and breastfeeding information was self-reported by parents. Results Skin carotenoids were inversely related to %Fat (P &lt; 0.01), VAT (P &lt; 0.01) and BMI %ile (P &lt; 0.01). VAT and BMI %ile significantly mediated this relationship between exclusive breastfeeding duration and skin carotenoids, following adjustment for dietary carotenoids, energy intake, and mother education. Conclusions Weight status and adipose tissue distribution mediate the positive correlation between exclusive breastfeeding duration and skin carotenoids among children aged 7–12 years. The results indicate the need to support breastfeeding and healthy physical growth in childhood for optimal carotenoid status.


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