Effects of electric charge on hydraulic conductivity of pulmonary interstitium

1991 ◽  
Vol 70 (5) ◽  
pp. 1928-1932 ◽  
Author(s):  
S. J. Lai-Fook ◽  
L. V. Brown
Keyword(s):  
Hydraulic Conductivity ◽  
Pulmonary Artery ◽  
Flow Rate ◽  
Electric Charge ◽  
Protamine Sulfate ◽  
Flow Ratio ◽  
All Solutions ◽  
Charged Membrane ◽  
Isolated Segment ◽  
Positively Charged

The hydraulic conductivity of pulmonary interstitium was measured in a short isolated segment of interstitium surrounding a large pulmonary artery (1-3 mm diam) of the rabbit. The flow rate of the following solutions was measured sequentially: normal saline, polycation protamine sulfate (0.08 mg/ml), cationic dextran (0.1 or 1.5%) or anionic dextran (0.1 or 1.5%), and hyaluronidase (testes, 0.02%) solution. The pH of all solutions was adjusted to 7.35-7.40. The ratios of the flow of protamine sulfate and cationic dextran to that of saline averaged 2.3 +/- 0.92 (SD, n = 7) and 3.0 +/- 1.2 (n = 6), respectively. The anionic dextran-to-saline flow ratio averaged 0.72 +/- 0.28 (n = 13). Flow increased in the presence of positively charged molecules and decreased in the presence of negatively charged molecules. At a lower pH of 5.0-6.0, only 0.1% cationic dextran had an effect on interstitial conductivity. Thus pulmonary interstitium at physiological pH has the properties of a negatively charged membrane. The increased interstitial conductivity caused by the positively charged molecules was not observed after treatment with hyaluronidase. These effects of electric charge on interstitial conductivity were partly attributed to the presence in the interstitium of negatively charged hyaluronan.

Effect of albumin on hydraulic conductivity of pulmonary artery endothelial monolayers

1991 ◽  
Vol 260 (6) ◽  
pp. L571-L576 ◽  
Author(s):  
B. K. McCandless ◽  
M. R. Powers ◽  
J. A. Cooper ◽  
A. B. Malik
Keyword(s):  
Hydraulic Conductivity ◽  
Pulmonary Artery ◽  
Water Flux ◽  
Vascular Wall ◽  
Caproic Acid ◽  
Unit Surface Area ◽  
Endothelial Monolayers ◽  
Unit Surface ◽  
The Media ◽  
Positively Charged

We tested the hypothesis that albumin reduces the vascular wall hydraulic conductivity by an interaction with the endothelium. The system consisted of luminal and abluminal chambers separated by a microporous filter onto which was grown a confluent monolayer of ovine pulmonary artery endothelial cells. The abluminal chamber filtrate was collected for timed periods during increases in transendothelial pressures of 5, 10, 15, and 20 cmH2O. The transendothelial water flux was linearly related to the hydrostatic pressure. Hydraulic conductivity (Lp) was determined from the slope of this relationship per unit surface area. In the absence of albumin, Lp of the endothelium and the filter was 14.8 +/- 3.8 x 10(-5) cm.s-1.cmH2O-1. The addition of either 2.5 or 5.0 mg/ml ovine serum albumin to the medium reduced Lp values similarly to 2.0 +/- 0.3 x 10(-5) and 2.5 +/- 1.1 x 10(-5) cm.s-1.cmH2O-1, respectively. Removal of albumin from the media reversed the effect of albumin on Lp. The filter Lp value of 3.2 +/- 0.3 x 10(-3) cm.s-1.cmH2O-1 was unaffected by albumin. Endothelial Lp value did not decrease with 5.0 mg/ml of 70-kDa neutral dextran. Albumin decreased Lp in the presence of epsilon-amino-caproic acid to the same extent as albumin alone, suggesting that the positively charged lysine sites on albumin did not mediate the effect. The results indicate that albumin decreases Lp due to an interaction between albumin and the endothelial cell.

Experimental Investigation of Effect of Tip Coolant Ejection on Internal Flow Characteristics Within a Realistic Blade Coolant Channel

2013 ◽  
Author(s):  
Jian Pu ◽  
Zhaoqing Ke ◽  
Jianhua Wang ◽  
Lei Wang ◽  
Hongde You
Keyword(s):  
Experimental Investigation ◽  
Reynolds Number ◽  
Pressure Drop ◽  
Turbine Blade ◽  
Flow Rate ◽  
Flow Characteristics ◽  
Reynolds Numbers ◽  
Flow Ratio ◽  
Lp Turbine ◽  
Mass Flow Ratio

This paper presents an experimental investigation on the characteristics of the fluid flow within an entire coolant channel of a low pressure (LP) turbine blade. The serpentine channel, which keeps realistic blade geometry, consists of three passes connected by a 180° sharp bend and a semi-round bend, 2 tip exits and 25 trailing edge exits. The mean velocity fields within several typical cross sections were captured using a particle image velocimetry (PIV) system. Pressure and flow rate at each exit were determined through the measurements of local static pressure and volume flow rate. To optimize the design of LP turbine blade coolant channels, the effect of tip ejection ratio (ER) from 180° sharp bend on the flow characteristics in the coolant channel were experimentally investigated at a series of inlet Reynolds numbers from 25,000 to 50,000. A complex flow pattern, which is different from the previous investigations conducted by a simplified square or rectangular two-pass U-channel, is exhibited from the PIV results. This experimental investigation indicated that: a) in the main flow direction, the regions of separation bubble and flow impingement increase in size with a decrease of the ER; b) the shape, intensity and position of the secondary vortices are changed by the ER; c) the mass flow ratio of each exit to inlet is not sensitive to the inlet Reynolds number; d) the increase of the ER reduces the mass flow ratio through each trailing edge exit to the extent of about 23–28% of the ER = 0 reference under the condition that the tip exit located at 180° bend is full open; e) the pressure drop through the entire coolant channel decreases with an increase in the ER and inlet Reynolds number, and a reduction about 35–40% of the non-dimensional pressure drop is observed at different inlet Reynolds numbers, under the condition that the tip exit located at 180° bend is full open.

scholarly journals Electrostatic Interaction Between NS1 and Negatively Charged Lipids Contributes to Flavivirus Replication Organelles Formation

2021 ◽  
Vol 12 ◽  
Author(s):  
Yali Ci ◽  
Yang Yang ◽  
Caimin Xu ◽  
Cheng-Feng Qin ◽  
Lei Shi
Keyword(s):  
Electrostatic Interaction ◽  
Membrane Binding ◽  
Essential Factor ◽  
Charged Residue ◽  
Charged Membrane ◽  
Phosphatidylinositol Phosphates ◽  
Positively Charged ◽  
Er Membrane

Flavivirus replication occurs in membranous replication compartments, also known as replication organelles (ROs) derived from the host ER membrane. Our previous study showed that the non-structural (NS) protein 1 (NS1) is the essential factor for RO creation by hydrophobic insertion into the ER membrane. Here, we found that the association of NS1 with the membrane can be facilitated by the electrostatic interaction between NS1 and negatively charged lipids. NS1 binds to a series of negatively charged lipids, including PI4P, and a positively charged residue, R31, located on the membrane-binding face of NS1, plays important roles in this interaction. The NS1 R31E mutation significantly impairs NS1 association with negatively charged membrane and its ER remodeling ability in the cells. To interfere with the electrostatic interaction between NS1 and negatively charged lipids, intracellular phosphatidylinositol phosphates (PIPs) level was downregulated by the overexpression of Sac1 or treatment with PI3K and PI4K inhibitors to attenuate flavivirus replication. Our findings emphasize the importance of electrostatic interaction between NS1 and negatively charged lipids in flavivirus RO formation.

scholarly journals Comparison of Different Synthesis Schemes for Production of Sodium Methoxide from Methanol and Sodium Hydroxide

2020 ◽  
Vol 24 (6) ◽  
pp. 63-77
Author(s):  
Natthiyar Aeamsuksai ◽  
Thirawat Mueansichai ◽  
Pongtorn Charoensuppanimit ◽  
Pattaraporn Kim-Lohsoontorn ◽  
Farid Aiouache ◽  
...  
Keyword(s):  
Energy Consumption ◽  
Total Energy ◽  
Flow Rate ◽  
Sodium Methoxide ◽  
Distillation Column ◽  
Reactive Distillation ◽  
Flow Ratio ◽  
Total Energy Consumption ◽  
Pervaporation Membrane ◽  
Simulation Parameters

This research investigates the process simulation of sodium methoxide (NaOCH3) synthesis from methanol (CH3OH) and sodium hydroxide (NaOH) under three synthesis schemes: schemes A, B, and C. Scheme A consisted of one equilibrium reactor and two distillation columns, scheme B one reactive distillation column and one distillation column, and scheme C one reactive distillation column and pervaporation membrane. The simulation parameters included CH3OH/NaOH feed flow ratio (1.2-1.6), number of stages (5-30), bottom flow rate (1400-1600 kg/h), and feed stage location (5, 10, 15, 20, 21, 22, 23, and 24). The simulation parameters were varied to determine the optimal NaOCH3 synthetic conditions under different schemes with 0.01 wt% water content, maximum 45 wt% NaOCH3, and the lowest total energy consumption. The results showed that scheme C had the lowest total energy consumption (34.25 GJ/h) under the optimal synthetic condition of 1.4 for CH3OH/NaOH feed flow ratio, 25 for the number of stages, 1550 kg/h for the bottom flow rate, and the 24th feed stage location, with the NaOCH3 flow rate of 675 kg/h. Scheme C thus holds promising potential as an energy-efficient alternative for synthesis of NaOCH3. The novelty of this research lies in the use of pervaporation membrane in place of distillation column to separate CH3OH from water and to lower energy consumption and capital cost.

scholarly journals Effect of Differentiated Injection Ratio, Gas Flow Rate, and Slag Thickness on Mixing Time and Open Eye Area in Gas-Stirred Ladle Assisted by Physical Modeling

Metals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 555 ◽  
Author(s):  
Luis E. Jardón-Pérez ◽  
Daniel R. González-Morales ◽  
Gerardo Trápaga ◽  
Carlos González-Rivera ◽  
Marco A. Ramírez-Argáez
Keyword(s):  
Flow Rate ◽  
Gas Flow ◽  
Mixing Time ◽  
Flow Ratio ◽  
Gas Flow Rate ◽  
Nsga Ii ◽  
Image Velocimetry ◽  
Gas Flow Ratio ◽  
Slag Thickness ◽  
Injection Ratio

In this work, the effects of equal (50%/50%) or differentiated (75%/25%) gas flow ratio, gas flow rate, and slag thickness on mixing time and open eye area were studied in a physical model of a gas stirred ladle with dual plugs separated by an angle of 180°. The effect of the variables under study was determined using a two-level factorial design. Particle image velocimetry (PIV) was used to establish, through the analysis of the flow patterns and turbulence kinetic energy contours, the effect of the studied variables on the hydrodynamics of the system. Results revealed that differentiated injection ratio significantly changes the flow structure and greatly influences the behavior of the system regarding mixing time and open eye area. The Pareto front of the optimized results on both mixing time and open eye area was obtained through a multi-objective optimization using a genetic algorithm (NSGA-II). The results are conclusive in that the ladle must be operated using differentiated flow ratio for optimal performance.

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