scholarly journals Clinical and Enzymatic Investigation of Induction of Oxygen Free Radicals by Ischemia and Reperfusion in Human Hepatocellular Carcinoma and Adjacent Liver

HPB Surgery ◽  
1995 ◽  
Vol 8 (3) ◽  
pp. 193-199 ◽  
Author(s):  
Akira Yamanoi ◽  
Naofumi Nagasue ◽  
Hitoshi Kohno ◽  
Takeo Kimoto ◽  
Terushisa Nakamura
Keyword(s):  
Hepatocellular Carcinoma ◽  
Free Radicals ◽  
Liver Tissue ◽  
Oxygen Free Radicals ◽  
Human Subjects ◽  
Thiobarbituric Acid ◽  
Mean Value ◽  
Ischemia And Reperfusion ◽  
Before And After ◽  
The Mean

Serum concentration of thiobarbituric acid (TBA) reactants in the hepatic vein were measured before and after transient dearterialization of the liver in five human subjects bearing unresectable hepatocellular carcinoma (HCC). During 1 hour of the occlusion of the hepatic artery, change inTBA reactants level was slight. However, the mean value of TBA reactants in 1 hour after the reflow increased to 1.50 ± 0.11 nmol/ml (mean ± S.E.) and was significantly higher (p < 0.05) than those before hepatic dearterialization (1.28 ± 0.11 nmol/ml) and just before the release of occlusion (1.32 ± 0.09 nmol/ml).Further, two endogeneous scavenger enzymes, superoxide dismutase (SOD) and catalase (CAT), and one of the major sources of oxygen free radicals, xanthine oxidase (XOD) were measured in human untreated HCC and the corresponding adjacent liver tissue. The results demonstrated an increase in SOD in 81.8% (9/11) of HCC, and a decrease in CAT in 72.7% (8/11) of HCC when compared with the corresponding adjacent liver tissue. The mean value of SOD in HCC was significantly higher (66.8 ± 6.5 vs 52.8 ± 3.8 U/mg protein; p < 0.05), and that of CAT was significantly lower (22.6 ± 2.4 vs 36.0 ± 6.1 U/mg protein; p < 0.05) than those in liver tissue. All of nine HCC samples had a significantly lower activity of XOD (6.4 ± 1.9 vs 20.3 ± 3.4 pmol/minute/mg protein; p < 0.01) than the corresponding liver tissue. There was no obvious relation between the content of SOD and CAT in HCC, or in liver tissue.These data may suggest that oxygen free radicals can be generated in human HCC by ischemia and reperfusion of the tumor- bearing liver. It is also indicated that the antioxidant system of HCC is not always impaired, and that HCC might develop several lines of defence systems against the oxidative attack. A possible strategy of the treatment for liver tumor with oxygen derived free radicals induced by ischemia and reperfusion is hypothized here.

scholarly journals Anticoagulation in Cardiopulmonary Bypass

1977 ◽  
Author(s):  
C. Thomas Kisker ◽  
John A. Young ◽  
Donald B. Doty ◽  
Barbara J. Taylor
Keyword(s):  
Cardiopulmonary Bypass ◽  
Antithrombin Iii ◽  
Human Subjects ◽  
Mean Value ◽  
Clotting Time ◽  
Excessive Bleeding ◽  
Safe Level ◽  
Activated Clotting Time ◽  
Before And After ◽  
The Mean

Prolonging the activated clotting time (ACT) 2 to 3 times normal is said to provide a “safe” level of anticoagulation during cardiopulmonary bypass. To test this level of anticoagulation 9 monkeys were anticoagulated with heparin at the start of cardiopulmonary bypass so that ACT’s ranged from 201 sec to > 1000 sec (normal 91 sec). ACT, platelet count (P), fibrinogen (F), and fibrin monomer (FM) were measured at 10, 30, 60, 90, and 120 minutes during bypass. Antithrombin III (AT3) was measured before and after bypass. Six monkeys developed increased FM indicating active coagulation beginning from 10 to 60 minutes on bypass. ACT’s were > 200 sec in all animals at the time of FM detection. P fell below 100,000/mm3 in the 6 animals with elevated FM, but remained above 100,000/mm3 in the other 3 animals. The mean value of AT3 (69%) decreased to 24.4% after bypass in the 6 animals with elevated FM, but was 61% after bypass in the others. Scanning electron microscopy of the oxygenator membranes showed significant amounts of fibrin on the membranes used in monkeys who developed increased FM levels, but not on those with normal FM concentrations. F decreased from 167 mgm/dl to 80.5 mgm/dl in monkeys with elevated FM and to 117 mgm/dl in those with normal FM concentrations. Excessive bleeding did not occur in the animals without increased FM although ACT’s were in excess of 1000 sec. Subsequently three human subjects on cardiopulmonary bypass whose ACT’s were maintained above 400 sec have not shown increased FM levels. The results suggest that prolonging the ACT more than 2 - 3 times normal is required to prevent activation of clotting during cardiopulmonary bypass.

Detection of Soluble Fibrin Monomer Complexes in Blood by Means of Protamine Sulphate Test

1968 ◽  
Vol 20 (01/02) ◽  
pp. 044-049 ◽  
Author(s):  
B Lipiński ◽  
K Worowski
Keyword(s):  
Human Subjects ◽  
Coronary Thrombosis ◽  
Mean Value ◽  
Healthy Human ◽  
Protamine Sulphate ◽  
Soluble Fibrin ◽  
Fibrin Monomer ◽  
Dog Plasma ◽  
The Mean ◽  
Precipitable Protein

SummaryIn the present paper described is a simple test for detecting soluble fibrin monomer complexes (SFMC) in blood. The test consists in mixing 1% protamine sulphate with diluted oxalated plasma or serum and reading the optical density at 6190 Å. In experiments with dog plasma, enriched with soluble fibrin complexes, it was shown that OD read in PS test is proportional to the amount of fibrin recovered from the precipitate. It was found that SFMC level in plasma increases in rabbits infused intravenously with thrombin and decreases after injection of plasmin with streptokinase. In both cases PS precipitable protein in serum is elevated indicating enhanced fibrinolysis. In healthy human subjects the mean value of OD readings in plasma and sera were found to be 0.30 and 0.11, while in patients with coronary thrombosis they are 0.64 and 0.05 respectively. The origin of SFMC in circulation under physiological and pathological conditions is discussed.

Perbedaan Pengetahuan Siswa Tentang Penyakit Hepatitis A Sebelum Dan Sesudah Penyuluhan Di MTsN 02 Bondowoso Tahun 2015

2017 ◽  
Vol 4 (2) ◽  
pp. 14
Author(s):  
Putri Megasari
Keyword(s):  
Hepatitis A ◽  
Hepatitis Virus ◽  
Sampling Technique ◽  
Mean Value ◽  
Test Design ◽  
The World ◽  
Before And After ◽  
Post Test ◽  
The Mean ◽  
Paired T Test

Hepatitis has become a health problem in the world. The hepatitis virus infected many people. According to the teacher of MTsN 02 Bondowoso more than 20 students have hepatitis A viral infection. The purpose of this research was to know the differences of students' knowledge about hepatitis A before and after counseling in MTsN 02 Bondowoso 2015. This study used pre-experimental (pre-post test design). This study used stratified random sampling technique, 127 students from 270 sample involved this research,and 143 students was excluded. We used questionnaires to collect data. The results showed that the mean value of the students 'knowledge about hepatitis A before counseling in MTsN 02 Bondowoso 2015 was 83.96 with the lowest value of 37.5 and the highest value was 100. The mean value of the students' knowledge about hepatitis A after counseling in MTsN 02 Bondowoso 2015 was 93.21 with the lowest value waf 62.5 and the highest value was 100. Paired t test showed that t (-9.07) > t table (1.98), the null hypothesis (H0) was rejected. There was a difference between students' knowledge about hepatitis A before and after counseling in MTsN 02 Bondowoso 2015. This study showed that routine counseling by healthcare provider was important to prevent hepatitis A infection.; Keywords: counseling, knowledge of students, hepatitis

scholarly journals Quality of life of obstetrics fistula patients before and after surgical repair in the Jimma University Medical Center, Southwest Ethiopia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tilahun Fufa Debela ◽  
Zerihun Asefa Hordofa ◽  
Aster Berhe Aregawi ◽  
Demisew Amenu Sori
Keyword(s):  
Quality Of Life ◽  
Surgical Repair ◽  
Obstetric Fistula ◽  
Medical Center ◽  
Mean Value ◽  
Significant Difference ◽  
Before And After ◽  
The Mean ◽  
Jimma University

Abstract Background The consequences of obstetric fistula on affected women are more than the medical condition. It has extensive physical, psychological, social, and economic consequences on them. Obstetric fistula affects the entire health and entire life of women. Women suffering from obstetric fistula are often abandoned by her partner, relatives, and the community. This study aimed to determine the quality of life of obstetrics fistula patients before and after surgical repair. Methods Institutional-based prospective, before and after study design was conducted in the Jimma University Medical Center from November 1, 2019–October 30, 2020. A face-to-face interview was conducted with fistula patients who visited Jimma University Medical center, fistula clinic during the study period. All fistula patients were included in the study. Accordingly, 78 women who underwent surgical repair were interviewed. The means and the standard deviation were computed using conventional statistics formulas. The unpaired t-test was used to compare two independent means, and one-way analysis of variance (ANOVA) was used to compare the quality of life before repair and after a successful repair. Linear regression analysis was done for identifying determinants of quality of life. A P value of 0.05 will be considered statistical significance. Result The overall quality of life of women was 58.17 ± 7.2 before the surgical repair and 71.20 ± 10.79 after surgical repair. The result indicates there is a significant difference in the mean value of pre and post-operative (P < 0.001). The overall satisfaction of women with their health status before the surgical repair was 22.5 ± 1.30and it has increased to 53.0 ± .90after surgical repair. The physical health dimension score was 16.51 ± 5.27 before the surgical repair while it has increased to 21.77 ± 5.38 after the surgical repair. The score of the social domain before the surgical repair was 5.19 ± 1.34 and it has increased to 7.13 ± 3.67 after the surgical repair. The score of the environmental health domain was 17.41 ± 2.89 before the surgery while it also increased to 21.65 ± 4.04 after the surgical repair. The results have shown there was a significant difference in the mean values of pre and post-operatives in both social and environmental scores (P < 0.001). The score of the psychological health domain before the surgery was 19.06 ± 1.46 and it was increased to 19.84 ± 3.21 after the surgical repair. The result showed there is a significant difference in mean value pre and post-operative (P = 0.048), though it is a slight improvement compared to other domains. Conclusion The overall quality of life of the patient with fistula was improved after successful surgical repair. Although all domains of quality of life had shown significant improvement after successful surgical repair, the psychological domain showed slight improvement.

scholarly journals The penetration of methanol into bovine cardiac and hepatic tissues is faster than ethanol and formalin

2018 ◽  
Author(s):  
Michelle Steicke ◽  
Guang Yang ◽  
Tam Nguyen Dinh ◽  
Matthew Dunster-Jones ◽  
Owen Sargisson ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Penetration Rate ◽  
Biological Tissues ◽  
Heart Tissue ◽  
Penetration Distance ◽  
Before And After ◽  
Tissue Shrinkage ◽  
The Mean ◽  
Post Hoc ◽  
Liver Tissues

Methanol, ethanol and formalin are commonly used as fixatives to preserve biological tissues from decay in the preparation of histological sections. Fixation of the inner layers of the tissue depends on the ability of the fixative to diffuse into the tissue. It is unknown whether methanol penetrates tissues at similar rates to other fixatives. This study aimed to compare the penetration rates of methanol, ethanol and formalin into bovine heart and liver tissues. The penetration distance and tissue shrinkage or expansion were measured by analysing the digital images of tissue before and after immersion in different fixatives for 1, 2, 6 or 10 h. Data were analysed using two-way ANOVA, followed by Bonferroni’s post-hoc test. The penetration distance of methanol was significantly greater in both heart and liver tissues compared with that of ethanol (N=4, P<0.001). Methanol or ethanol immersion led to similar shrinkage of both tissues (P>0.05). The penetration rate of formalin was similar to that of ethanol in both tissues however it was significantly slower than methanol (N=4, P<0.005 in the heart; P<0.001 in the liver). The mean penetration coefficients of methanol, formalin and ethanol in the heart tissue were 2.609, 1.994 and 1.801, respectively, and 3.012, 2.153 and 2.113, respectively, in the liver tissue. The penetration coefficient of methanol was significantly greater than that of ethanol or formalin in both tissues (P<0.001 for each comparison). In conclusion, methanol penetrates tissue significantly faster than ethanol and formalin.

scholarly journals Effect of Tea Tree Oil on Improvement of Hair Damage

2021 ◽  
Vol 19 (3) ◽  
pp. 333-342
Author(s):  
Ju-Sub Kim
Keyword(s):  
Tensile Strength ◽  
Color Difference ◽  
Mean Value ◽  
Tea Tree Oil ◽  
Tea Tree ◽  
Natural Extracts ◽  
Hair Samples ◽  
Before And After ◽  
L Value ◽  
The Mean

Purpose: The study aimed to investigate the effects of tea tree oil on damaged hair.Methods: A texture-improving agent was produced by adding different dosages of tea tree oil (i.e., 0, 2, 4, and 6 g) on sample hair. Comparative analysis was conducted by measuring the effects of the agent before and after application to sample hair bleached into level 8. To examine changes in hair brightness, L* value was measured using a color difference meter. In addition, to determine the effects of the agent on damaged hair, the study measured tensile strength, absorbance using methylene blue, and hair gloss.Results: The mean L* value for each sample increased for healthy hair but decreased in the 8L samples. Analysis indicates that the healthy hair, 8L(4) sample, and 8L(6) samples pointed to significant results. The mean value for tensile strength increased for all healthy and 8L hair samples after application. Specifically, the 8L(4) and 8L(6) samples displayed significant results. In terms of absorbance, the mean value decreased for all healthy and 8L hair samples after application. Moreover, hair gloss values increased for samples applied with the agent containing tea tree oil. Specifically, the 8L(2), 8L(4), and 8L(6) samples exhibited increased values after application.Conclusions: The results confirm the effects of tea tree oil on damaged hair in terms of improvements in terms of tensile strength, absorbance, and gloss. The study recommends that further research on the effects of various oils and natural extracts on damaged hair is necessary in terms of improving other hair characteristics and using more samples.

scholarly journals Semen characteristics of breeding bulls at the Central Cattle Breeding and Dairy Farm of Bangladesh

2012 ◽  
Vol 41 (1) ◽  
pp. 1-5
Author(s):  
ME Hossain ◽  
MM Khatun ◽  
MM Islam ◽  
OF Miazi
Keyword(s):  
Sperm Concentration ◽  
Chemical Properties ◽  
Dairy Farm ◽  
Mean Value ◽  
Cattle Breeding ◽  
Bull Semen ◽  
Before And After ◽  
The Mean ◽  
Physical And Chemical ◽  
And Chemical Properties

The experiment was conducted on 97 breeding bulls at the central cattle breeding and dairy farm, Savar, Dhaka to find out the physical and chemical properties of different bull semen. Out of 97 bulls, 9 were Local (L), 9 were Friesian (F), 13 were Sahiwal (SL), 12 were Local×Friesian (L×F), 10 were Sahiwal×Friesian (SL×F), 26 were Local×Friesian×Friesian (LF1×F), 18 were Local×Friesian× Friesian×Friesian (LF2×F) bulls. The semen of different crossbred and local bulls was examined for ejaculate volume, concentration, pH and motility percent of sperm before and after freezing. It was observed that, the maximum average ejaculate was obtained from SL×F and the mean value was 12.9 ml. However, the minimum average ejaculate was obtained from LF×F and the mean value was 7.4 ml. Before freezing, the maximum average sperm concentration was obtained from SL and the mean value was 1858.4 million/ml. The minimum average sperm concentration was obtained from LF1×F and the mean value was 1286.6 million/ml. The maximum average pH was obtained from LF2×F and the mean value was 6.5. The maximum average motility was obtained from SL and the mean value was 68.8%. However, the minimum average motility was obtained from L×F and the mean value was 63.7%. After freezing, sperm concentration, pH and motility reduced substantially irrespective of type of breed. It could therefore be inferred that freezing of semen should be undertaken with special care to maintain optimum semen quality.http://dx.doi.org/10.3329/bjas.v41i1.11968

scholarly journals ANALISIS PERBEDAAN ABNORMAL RETURN DAN TRADING VOLUME ACTIVITY SAHAM SEBELUM DAN SESUDAH PERISTIWA GEMPA DI YOGYAKARTA TANGGAL 27 MEI 2006 (Sudi Kasus pada Perusahaan Asuransi Terdaftar di Bursa Efek Jakarta)

IQTISHODUNA ◽  
2011 ◽  
Vol 2 (2) ◽  
Author(s):  
Lulu Nurul Istanti, SE., MM.,
Keyword(s):  
Abnormal Return ◽  
Trading Volume ◽  
Stock Exchange ◽  
Mean Value ◽  
Volume Activity ◽  
Significant Difference ◽  
Before And After ◽  
Insurance Firms ◽  
The Mean ◽  
Difference Test

This research presents an empirical analysis of difference between abnormal return and trading volume activity before and after earths-quake, in Yogyakarta at May 27, 2006. And examine its statistical properties. This research argues that there was difference between abnormal return and trading volume activity before and after quake. For this purpose, the mean difference test, using t-test, was applied to compare the mean value of abnormal return and trading volume activity before and after quake. The sample of this research consists of the insurance firms listed at the Jakarta Stock Exchange. Investigation on the sample firms involved periods of ten days before quake and ten days after quake. The results of this research indicate that there was no significant difference between the abnormal return and trading volume activity before and after quake. This evidence confirms that even did not positively influence abnormal return and trading volume activity as suggested theoretically.  

scholarly journals Effects Of Home-Based Convergence Insufficiency Vision Therapy On Accommodation Among School Going Children

2020 ◽  
Vol 7 (06) ◽  
pp. 4848-4853
Author(s):  
Andrew Wekesa ◽  
Mazximila Wanzala
Keyword(s):  
Mean Value ◽  
Mean Values ◽  
Convergence Insufficiency ◽  
Home Based ◽  
Before And After ◽  
The Mean ◽  
Home Based Therapy ◽  
Vision Therapy ◽  
The Town ◽  
Accommodative Facility

Effects of home-based convergence insufficiency vision therapy on accommodation among school going children Wekesa Andrew1, Wanzala Maximilla1 1Masinde Muliro University of Science and Technology Kakamega, Kenya.   Abstract Background: Convergence and accommodation are always yoked together and any adjustment on the former affects the later. The most effective treatment for convergence insufficiency (CI) is vision therapy. Aim/Purpose: To determine the effects of home-based vision therapy on accommodation in school-going with CI attending the Masinde Muliro University Academic Vision Center in Kenya. Methods: A clinical experimental design involving 23 participants with the mean age of 14±2.4 years, were recruited into the study, however, only 18 were assessed after the therapy. The study took 9 weeks thus majority lost contact while others moved out of the town. Home-based vision therapy which is undertaken at home using a pencil or broke strings. During the therapy, accommodative values were monitored which were; negative relative accommodation, positive relative accommodation, dynamic accommodation, accommodative facility and near point of accommodation. Paired t-test used to compare mean values before and after the therapy. Results: The mean value of negative relative accommodation (NRA) before (mention what the mean values were here) and after (mention what the mean values were here) home-based therapy was statistically significant (p=0.01). However, the mean values before and after home-based therapy for the near point of accommodation (NPA), dynamic (Lag), relativity (PRA) and facility (MAF) showed no difference (p> 0.05). The mean value of the NRA before and after office-based vision therapy was statistically significant (p=0.01). Conclusion: The use of home-based vision therapy for the patients with CI had significant effects on accommodation changing from a lower limit to average after the therapy.   Keywords: Convergence insufficiency, Accommodation, Home-based vision therapy.

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