scholarly journals Time-Resolved Fluorometry Based Sandwich Hybridisation Assay for HLA-DQA1 Typing

1998 ◽  
Vol 14 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Minna Sjöroos ◽  
Jorma Ilonen ◽  
Helena Reijonen ◽  
Timo Lövgren
Keyword(s):  
Hla Typing ◽  
Time Resolved Fluorescence ◽  
Specific Detection ◽  
Time Resolved ◽  
Exon 2 ◽  
Pcr Product ◽  
Detection Probe ◽  
Hybridisation Signal ◽  
Hla Dqa1 ◽  
Biotinylated Probes

A microtitration plate based time-resolved fluorescence (TRF) hybridisation assay was developed for HLA typing utilising biotinylated sequence-specific catching probes and europium (Eu) labelled gene locus-specific detection probe to allow time-resolved fluorometer reading of the reaction. In an application for HLA-DQA typing a 228 base pair long region of the polymorphic exon 2 of DQA1 gene was amplified and the denatured PCR product distributed into streptavidin-coated microtitration wells together with the detection probe and one of the catching probes. After incubation and washes, the enhancement solution was added and specific hybridisation signal detected by measuring the emitted light. A series of 100 isolated genomic DNA samples were studied using biotinylated probes specific for DQA1*01, *0101/0104, *0103/0201/0601, *0201, *03, *0401/0601, *05 and *0502 alleles with results demonstrating the capacity of the test to detect aimed alleles. A series of whole blood spot samples were also studied and the results confirmed the applicability of this modification of the test.

scholarly journals A Luminescent, Water-Soluble Ir(III) Complex as a Potential Photosensitizer for Two-Photon Photodynamic Therapy

2021 ◽  
Vol 11 (24) ◽  
pp. 11596
Author(s):  
Elisabeta I. Szerb ◽  
Sharmistha Chatterjee ◽  
Massimo La Deda ◽  
Giovanna Palermo ◽  
Lucie Sancey ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Soluble ◽  
In Vitro Experiments ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Biologically Relevant ◽  
Two Photon ◽  
Fluorescence Measurements ◽  
Detection Probe

This work reports the study of two-photon induced properties of a highly luminescent cyclometalated Ir(III) complex, [Ir(ppy)2(en)] OOCCH3 (1), ppy = 2-phenylpyridine, en = ethylenediamine. Steady-state and time-resolved fluorescence measurements were performed by exciting 1 at the biologically relevant wavelength of 800 nm, whereas, the generation of singlet oxygen (1O2) was evaluated using 9,10-Anthracenediyl-bis(methylene)dimalonic acid (ABDA) as a detection probe. Preliminary in vitro experiments with U87-MG cells were performed, showing the potential of this compound as a two-photon photodynamic therapy (2P-PDT) agent at NIR wavelengths.

scholarly journals The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreea Lorena Mateescu ◽  
Nicolae-Bogdan Mincu ◽  
Silvana Vasilca ◽  
Roxana Apetrei ◽  
Diana Stan ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Protein Complexes ◽  
C Reactive Protein ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reactive Protein ◽  
In Vitro Diagnostic ◽  
The Stability ◽  
Positive Controls

AbstractThe purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C.

scholarly journals Architecture of Polyglutamine-containing Fibrils from Time-resolved Fluorescence Decay

2014 ◽  
Vol 289 (39) ◽  
pp. 26817-26828 ◽  
Author(s):  
Christoph Röthlein ◽  
Markus S. Miettinen ◽  
Tejas Borwankar ◽  
Jörg Bürger ◽  
Thorsten Mielke ◽  
...  
Keyword(s):  
Fluorescence Decay ◽  
Time Resolved Fluorescence ◽  
Time Resolved
Sign in / Sign up

Export Citation Format

Share Document