scholarly journals HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Daya L. Chothani ◽  
M. B. Patel ◽  
S. H. Mishra
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Mobile Phase ◽  
Ethyl Acetate Extract ◽  
Marker Compound ◽  
Biological Nature

The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366 nm) at : 0.56 (mobile phase chloroform : toluene : ethyl acetate (6 : 3 : 1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366 nm) at : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound.

A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC

2021 ◽  
pp. 2613-2618
Author(s):  
A Rajasekaran ◽  
V Abirami ◽  
S Arunkumar ◽  
Priya G Bharani ◽  
S Gugapriya ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Tamil Nadu ◽  
Leaf Extract ◽  
Major Constituent ◽  
Marker Compound ◽  
Aluminium Sheets ◽  
Hptlc Method

Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.

Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

2021 ◽  
Vol 20 ◽  
Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Petroleum Ether Extract ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
Membrane Stabilization ◽  
Leaf Extracts ◽  
Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

scholarly journals Comparative phytotoxicity and chironomid toxicity assessments of leaf successive extract fractions of Asiatic poison bulb, Crinum asiaticum L

2020 ◽  
Author(s):  
Souren Goswami ◽  
Sanjib Ray
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Meristematic Tissue ◽  
Aqueous Fraction ◽  
Extract Fraction ◽  
Potent Growth ◽  
Crinum Asiaticum

AbstractCrinum asiaticum is an evergreen bulbous perennial shrub of Amaryllidaceae family with ethnomedicinal importance and our earlier study described a comparative account antimicrobial and antioxidant properties of the different solvent-mediated sequential extract fractions. The present study aimed to analyze their comparative account of induced phytotoxicity and chironomid toxicity. For phytotoxicity assessment, germination inhibition and seedling’s root and shoot growth retardation effects on Triticum aestivum and Cicer arietinum were analyzed and for lethal concentration determination, the freshwater bottom-dwelling first instars chironomid larvae were used. The crude aqueous, petroleum ether and ethyl acetate extracts showed significant toxic effects on both meristematic tissue and aquatic midges. The phytotoxic assays indicate that the ethyl acetate fraction contains the most potent growth inhibitors, followed by the crude aqueous and petroleum ether fractions. The last aqueous fraction was found to be the least toxic, the highest LC50 and LT50 values and ethyl acetate extract fraction having highest toxicity. Thus the present study supplements to our earlier report, that indicated the last aqueous extract fraction of C. asiaticum has potent antioxidant and antibacterial potentials as well as its prospective use in livestock maintenance, as it is least toxic and the ethyl acetate extract, the most toxic fraction identified here, is needed to explore for pharmaceutical importance.

scholarly journals Spray-Drying Microencapsulation of High Concentration of Bioactive Compounds Fragments from Euphorbia hirta L. Extract and Their Effect on Diabetes Mellitus

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 881 ◽  
Author(s):  
Ngan Tran ◽  
Minh Tran ◽  
Han Truong ◽  
Ly Le
Keyword(s):  
Blood Glucose ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Petroleum Ether Extract ◽  
Fast Blood Glucose ◽  
Chloroform Extract ◽  
Ether Extract ◽  
Euphorbia Hirta

The present study was performed to spray-dry the high concentration of bioactive compounds from Euphorbia hirta L. extracts that have antidiabetic activity. The total phenolic content (TPC) and total flavonoid content (TFC) of four different extracts (crude extract, petroleum ether extract, chloroform extract and ethyl acetate extract) from the dried powder of Euphorbia hirta L. were determined using a spectrophotometer. After that, the fragment containing a high number of bioactive compounds underwent spray-dried microencapsulation to produce powder which had antidiabetic potential. The total phenolic content values of the crude extract, petroleum ether extract, chloroform extract and ethyl acetate extract were 194.55 ± 0.82, 51.85 ± 3.12, 81.56 ± 1.72 and 214.21 ± 2.53 mg/g extract, expressed as gallic acid equivalents. Crude extract, petroleum ether extract, chloroform extract and ethyl acetate extracts showed total flavonoids 40.56 ± 7.27, 29.49 ± 1.66, 64.99 ± 2.60 and 91.69 ± 1.67 mg/g extract, as rutin equivalents. Ethyl acetate extract was mixed with 20% maltodextrin in a ratio of 1:10 to spray-dry microencapsulation. The results revealed that the moisture content, bulk density, color characteristic, solubility and hygroscopicity of the samples were 4.9567 ± 0.00577%, 0.3715 ± 0.01286 g/mL, 3.7367 ± 0.1424 Hue, 95.83 ± 1.44% and 9.9890 ± 1.4538 g H2O/100 g, respectively. The spray powder was inhibited 51.19% α-amylase at 10 mg/mL and reduced 51% in fast blood glucose (FBG) after 4 h treatment. Furthermore, the administration of spray powder for 15 days significantly lowered the fast blood glucose level in streptozotocin-diabetic mice by 23.32%, whereas, acarbose—a standard antidiabetic drug—and distilled water reduced the fast blood glucose level by 30.87% and 16.89%. Our results show that obtained Euphorbia hirta L. powder has potential antidiabetic activity.

scholarly journals Fractionation and identification of some phenolics extracted from evening primrose seed meal

2008 ◽  
Vol 26 (No. 1) ◽  
pp. 58-64 ◽  
Author(s):  
L. Zahradníková ◽  
Š. Schmidt ◽  
Z. Sékelyová ◽  
S. Sekretár
Keyword(s):  
Ethyl Acetate ◽  
Mobile Phase ◽  
Total Phenolics ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Syringic Acid ◽  
Seed Meal ◽  
Oenothera Biennis ◽  
Evening Primrose ◽  
Ethyl Acetate Extracts

Crude ethanol and ethyl acetate extracts of evening primrose (<I>Oenothera biennis</I> L.) seed meal were separated into fractions using Silicagel L 100/200 column chromatography and methanol as the mobile phase. Six fractions were obtained from the ethanol extract and five fractions from the ethyl acetate extract and their contents of total phenolics were determined. By means of HPLC, the active compounds were identified as catechin, gallic acid, caffeic acid, syringic acid, and ferulic acid.

Liquid Chromatographic Determination of Simazine, Atrazine, and Propazine Residues in Catfish

1995 ◽  
Vol 78 (4) ◽  
pp. 1067-1071 ◽  
Author(s):  
David C Holland ◽  
Robert K Munns ◽  
José E Roybal ◽  
Jeffrey A Hurlbut ◽  
Austin R Long
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Triazine Herbicides ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the simultaneous determination of the triazine herbicides, simazine (SIM), atrazine (ATZ), and propazine (PRO) in the 12.5–100 ppb range in catfish. The herbicides are extracted from catfish homogenates with ethyl acetate, followed by solvent partitioning between acetonitrile and petroleum ether and additional cleanup on a C18 cartridge. A Supelcosil LC-18-DB column is used for LC separation, and UV determination is at 220 nm. The isocratic mobile phase is a mixture of methanol, acetonitrile, and water. Mean recoveries from catfish were 88.7, 96.9, and 91.7%; standard deviations were 6.84,7.78, and 6.26%; and coefficients of variation were 7.72,8.03, and 6.82% for SIM, ATZ, and PRO, respectively.

scholarly journals Phytochemical and Biological Studies on Bark Extract of Aporosa wallichii Hook.f.

2020 ◽  
Vol 19 (2) ◽  
pp. 139-143
Author(s):  
Kazi Ruhullah Shahriar ◽  
Sirajis Salekin ◽  
Quazi Sufia Islam ◽  
Monira Ahsan ◽  
Choudhury M Hasan
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Soluble Fraction ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Bark Extract ◽  
Separation And Purification ◽  
Biological Studies

The ethyl acetate extract of bark of Aporosa wallichii Hook.f. was extensively investigated for the chemical characterization of secondary metabolites and for biological activities. After repeated chromatographic separation and purification, two compounds were elucidated as a phenolic compound ferulic acid (1) and an uncommon triterpene glut-5(6)-en-3β-ol (2) from the ethyl acetate extract of barks of A. wallichii by using high field NMR analyses. This is the first report of isolation of both compounds from this plant species. No significant antimicrobial activity was observed for any fraction after Kupchan partitioning of the extract. During the antioxidant activity assay, the ethyl acetate soluble fraction of A. wallichii demonstrated significant DPPH radical scavenging capacity with an IC50 value of 1.25 μg/mL. The other fractions viz. petroleum ether, aqueous and chloroform soluble fractions exhibited moderate, mild, and weak activity respectively. The petroleum ether soluble fraction demonstrated maximum thrombolytic property (51.33%) which was similar to that of standard streptokinase (66.81%). Dhaka Univ. J. Pharm. Sci. 19(2): 139-143, 2020 (December)

Effect of Chroogomphis Rutillus Extracts on Activity of Nitrite Scavenging and Nitrosamine Formation Inhibition

2011 ◽  
Vol 282-283 ◽  
pp. 545-548
Author(s):  
Hong Fang Ji ◽  
Ling Wen Zhang ◽  
Hui Hui Jin ◽  
Ling Yu Wang ◽  
Fang Yuan Cheng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Water Extract ◽  
Dependent Manner ◽  
In Vitro Systems ◽  
Dose Dependent ◽  
Ethyl Acetate Extracts ◽  
Scavenging Rates

Chroogomphis rutillusextracts were prepared with solvent of petroleum ether, acetic ester, methanol and water. The effects of four different extract on nitrite scavenging (NS) and nitrosamine formation inhibiting (NFI) were evaluated in vitro systems. Results showed that all the extract showed NS and NFI potentials with a dose-dependent manner. With regard to NS activity, at 50μg/mL, the scavenging rates of ethyl acetate and methanol extracts were 96.65% and 93.48%, respectively. While, those of petroleum ether and water extract were only 86.96% and 26.09%, respectively. With regard to NFI activity,at 200μg/mL, both petroleum ether and ethyl acetate extracts showed excellent inhibition abilities of 94.46% and 91.33%, respectively, much higher than those of methanol and water extract (78.57% and 4.06%, respectively) (P<0.05).Chroogomphis rutillusespecially ethyl acetate extract may have a preventive effect against carcinogenesis induced by nitrosamines.

scholarly journals DETERMINATION OF ANNONACYN COMPOUND BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ON THE EXTRACT OF Annona muricata LINN SEED FOR PESTICIDE FORMULA

2010 ◽  
Vol 5 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Freddy L Wurangian
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Ethanolic Extract ◽  
Raw Material ◽  
Limit Detection

Determination of annonacyn grade, a main insecticide compound has been done in the ethanolic and ethyl acetate extracts produced by soxhletation, and ethyl acetate extract produced by fractionation of ethanolic extract to find out the most active extract to be used as raw material of pesticide formulae. Analysis method used the reverse phase high performance liquid chromatography with column of Novapack ODS C-18 (waters; 3.9 x 150 nm), mobile phase was the mixture of acetonitrile-water. The result of this research showed the optimum condition as follows: the mobile phase was acetonitrile-water (60:40), flow rate of 0.2 mL min-1, injection volume of 5.0 L, detector UV, wavelength () at 220 nm with AUFS of 1.00. The limit detection of annonacyn was 0.01 g, annonacyn grade in ethanolic extract produced by soxhletation: 0.0405 + 0.0021%, in ethyl acetate extract produced by soxhletation: 0.0293 + 0.0009%, in ethyl acetate extract produced by fractionation of ethanolic extract: 0.1003 + 0.0018%. The precision and accuracy of annonacyn in this research were respectively obtained 8.89% - 1.92%, and 4.91% - 7.28%, at the concentration of 5.00 g mL-1 - 25.00 g mL-1. The sensitivity was 0.75

scholarly journals PHYTOCHEMICAL SCREENING IN VITRO BIOLOGICAL ACTIVITIES AND ISOLATION OF ACTIVE MOLECULE FROM VITEX ALTISSIMA LEAVES

2020 ◽  
pp. 96-100
Author(s):  
PREETHI NAIDU V ◽  
VAGDEVI HM ◽  
LATHA KP ◽  
AJISH AD
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Bioactive Molecule ◽  
Ethanol Extracts

Objective: The objective of this work is to investigate the antibacterial, anthelmintic activity of the leaves of Vitex altissima and isolation of the bioactive molecule. Methods: The agar disk diffusion method is implemented to evaluate the antibacterial activity of the plant leaves of V. altissima, using petroleum ether, ethyl acetate, and ethanol extracts. Exactly 1 mg of each extract is dissolved in 1 ml of dimethyl sulfoxide. The circular Whatman filter paper of diameter 5 mm was dipped in each extract and placed over solidified agar medium. The zone of inhibition was measured. The petroleum ether, ethyl acetate, and ethanol extracts of the plant have been used to carry out the anthelmintic activity against Indian earthworms Pheretima posthuma. The column chromatography technique is used for the isolation of bioactive molecules. Results: The results revealed that the ethyl acetate extract exhibited a remarkable zone of inhibition against Gram-positive and Gram-negative bacteria. The petroleum ether, ethyl acetate, and ethanol extracts produce zero percentage zone of inhibition against Escherichia coli bacteria. The ethanol extract showed potent anthelmintic activity. The spectral data confirm that the structure of the bioactive molecule is 4-hydroxybenzoic acid. Conclusion: The preliminary results of the study revealed that the ethyl acetate extract of the plant exhibited a broad zone of inhibition against various Gram-positive and Gram-negative bacterial strains and ethanol extract showing significant anthelmintic activity. Spectral data confirmed the structure of the bioactive molecule. The obtained bioactive molecule is responsible for exhibiting potent antibacterial activity.

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