scholarly journals Production of Biomass-Degrading Multienzyme Complexes under Solid-State Fermentation of Soybean Meal Using a Bioreactor

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Gabriela L. Vitcosque ◽  
Rafael F. Fonseca ◽  
Ursula Fabiola Rodríguez-Zúñiga ◽  
Victor Bertucci Neto ◽  
Sonia Couri ◽  
...  
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Flow Rate ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Monitoring And Control ◽  
Xylanase Production ◽  
Operational Conditions ◽  
Initial Substrate ◽  
Substrate Moisture

Biomass-degrading enzymes are one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels. This work evaluates the effects of operational conditions on biomass-degrading multienzyme production by a selected strain of Aspergillus niger. The fungus was cultivated under solid-state fermentation (SSF) of soybean meal, using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The effects of air flow rate, inlet air relative humidity, and initial substrate moisture content on multienzyme (FPase, endoglucanase, and xylanase) production were evaluated using a statistical design methodology. Highest production of FPase (0.55 IU/g), endoglucanase (35.1 IU/g), and xylanase (47.7 IU/g) was achieved using an initial substrate moisture content of 84%, an inlet air humidity of 70%, and a flow rate of 24 mL/min. The enzymatic complex was then used to hydrolyze a lignocellulosic biomass, releasing 4.4 g/L of glucose after 36 hours of saccharification of 50 g/L pretreated sugar cane bagasse. These results demonstrate the potential application of enzymes produced under SSF, thus contributing to generate the necessary technological advances to increase the efficiency of the use of biomass as a renewable energy source.

Cellulase Production under Solid State Fermentation in Cashew Apple Bagasse by Trichoderma reesei LCB 48

2015 ◽  
Vol 365 ◽  
pp. 323-328
Author(s):  
Simone Aparecida da Silva Lins ◽  
Líbia de Sousa Conrado
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Initial Moisture Content ◽  
Cellulase Production ◽  
Cashew Apple Bagasse ◽  
Production Values ◽  
Initial Substrate ◽  
Substrate Moisture

Cellulases, among many enzymes, have been highlighted in several areas of expertise, such as food, textiles, pulp and paper and wastewater treatment of effluents and residues. There is also the challenge of producing biofuels, where currently cellulases have been widely applied in the production of cellulosic ethanol, where it is used during the stage of hydrolysis of lignocellulosic biomass for conversion of cellulose to glucose. Studies have been developed in order to produce this enzyme through a process of solid state fermentation from lignocellulosic agroindustrial wastes, thus reducing the cost of enzyme production, and adding value to the residue. The aim of this work was to produce cellulases from the stalk of the cashew bagasse using Trichoderma reesei LCB 48. The study of the cellulase production was performed using 22 factorial design with central point in quadruplicate. The washed stalk of the cashew bagasse inoculated with T. reesei was evaluated for the production of cellulases with initial moisture contents of 45, 55 and 65% and in the presence of inorganic nitrogen ((NH4)2SO4) at concentrations 0.5, 0.75 and 1%. The fermentation was developed over 238 hours, and during this period the parameters analyzed were pH, moisture, AR and enzyme activity expressed in CMCase. Peak production of cellulase enzyme expressed in CMCase was achieved with 238 hours which value was 0.71 U/g (0.095 U/mL), under the conditions of 45% initial moisture content and 1% of nitrogen source. This activity was obtained in only one stage of the biotechnological process, the solid state fermentation; the next ones are concentration and purification. The using of experimental design methodology allowed us to observe the initial substrate moisture is the determining variable in the production of enzymes CMCases, and the minimum moisture level (45%) showed the highest production values of CMCase.

scholarly journals Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation

2011 ◽  
Vol 29 (No. 5) ◽  
pp. 557-567 ◽  
Author(s):  
X.-J. Dai ◽  
M.-Q. Liu ◽  
H.-X. Jin ◽  
M.-Y. Jing
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Xylanase Activity ◽  
Orange Peel ◽  
Basic Medium ◽  
Relative Molecular Mass ◽  
Xylanase Production ◽  
Fermentation Time

The production of xylanase (XylA) by Aspergillus niger JL-15 in solid-state fermentation (SSF) on orange peel was optimised by the response surface methodology (RSM). The results revealed that four factors had significant effects on the XylA production (P &lt; 0.05), that is the concentrations of the added glycerin and ammonium sulfate, the moisture content, and fermentation time. Exploying orange peel as the solid substrate, maximum xylanase activity (917.7&nbsp;U/g dry fermentation product) was obtained at 4.2% glycerin, 3.1% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 61% moisture content, and 73.4-h fermentation, this activity being close to the predicted one and 3.2 times higher than that of the basic medium (218.5&nbsp;U/g). Optimum temperature and pH for XylA activity were 55&deg;C and pH 5.0, respectively. SDS-PAGE analysis showed that the relative molecular mass of XylA was about 30.0 kDa. XylA exhibited K<sub>m</sub> and V<sub>max </sub>values of 9.24 mg/ml and 54.05&nbsp;&mu;mol/min/ml, respectively. XylA liberated mainly xylotriose from birchwood xylan and wheat bran, respectively. XylA was an endo-acting xylanase with transglycosylation activity, with the ability to hydrolyse, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexaose.

scholarly journals Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 585 ◽  
Author(s):  
Rajeev Ravindran ◽  
Gwilym A. Williams ◽  
Amit K. Jaiswal
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Initial Moisture Content ◽  
Medium Composition ◽  
Xylanase Production ◽  
Box Behnken Design ◽  
Fermentation Conditions ◽  
Coffee Waste ◽  
Media Component

In this study, spent coffee waste (SCW) was used as the sole carbon source for xylanase production in solid state fermentation mode using Aspergillus niger. A Box–Behnken design was constructed using three parameters viz. temperature, initial moisture content, and log number of spores to determine the optimal fermentation condition. The best fermentation conditions for xylanase production were found to be incubation at 30 °C with an initial moisture content of 70% and using an inoculum of 6.5 × 106 spores/g of dry SCW. Furthermore, the design of experiments revealed that maintaining a medium composition of 0.2 g of yeast extract, 0.04 g of K2HPO4, and 0.03 g of MgSO4 increased xylanase production. Under optimised solid-state fermentation conditions an enzyme activity of 6495.6 IU/g of dry SCW was recorded, which was approximately 1.39-fold higher than that of control (4649 IU/g of dry SCW). The efficacy of the purified xylanase as a juice enrichment agent for strawberry, blueberry, and raspberry pulp was tested.

scholarly journals Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2015 ◽  
Vol 176 (1) ◽  
pp. 317-319 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

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