Association betweenIL17Polymorphisms and Risk of Cervical Cancer in Chinese Women

Interleukin-17 (IL-17) is a proinflammatory cytokine that is associated with inflammation, autoimmune disorders, and even tumors. Previous studies revealed that a large group of human malignant tumors have abnormally high IL-17 expression. In the present study, we analyzed two single-nucleotide polymorphisms (SNPs) in theIL17A(rs2275913) andIL17F(rs763780) in 311 cervical cancer patients and 463 controls using TaqMan assays. Our results indicated that the frequencies of AA genotype and A allele of rs2275913 were significantly different between the cervical cancer patients and controls (P=0.008, OR = 1.32, 95% CI, 1.07–1.62). Stratified analyses revealed that the polymorphism of rs2275913 was also associated with positive peritumor intravascular cancer emboli and high clinical stage. The genotype and allele frequencies of rs763780 did not show any difference between patients and controls or relate to patient clinical characteristics. Collectively, these findings suggested thatIL17gene polymorphism rs2275913 was associated with the susceptibility as well as positive peritumor intravascular cancer emboli and high clinical stage of cervical cancer in Chinese women.

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Association Between LIN28A Gene Polymorphisms and Glioma Susceptibility in Chinese Children

Cancer Control ◽

10.1177/10732748211040009 ◽

2021 ◽

Vol 28 ◽

pp. 107327482110400

Author(s):

Huiqin Guo ◽

Yuxiang Liao ◽

Ao Lin ◽

Huiran Lin ◽

Xiaokai Huang ◽

...

Keyword(s):

Gene Polymorphisms ◽

Case Control Study ◽

Malignant Tumors ◽

Clinical Stage ◽

Case Control ◽

Chinese Children ◽

Stage Iii ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Control Study

Gliomas are the most prevalent brain tumors among children and adolescents. The occurrence and development of various malignant tumors is closely related with LIN28A gene, but its relationship with glioma susceptibility has not been widely discovered. In this case-control study, we conducted four single nucleotide polymorphisms (SNPs) (rs3811464 G>A, rs3811463 T>C, rs34787247 G>A, and rs11247957 G>A) of LIN28A gene to investigate whether they increase the risk of glioma. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate their relationship. There was no significant correlation between four SNPs and glioma risk in single polymorphism and conjoint analysis. However, in stratification analysis, we found that rs3811463 TC/CC may add to the risk of glioma with clinical stage III (adjusted OR = 3.16, 95% CI = 1.15-8.70, P = .026) or stage III+IV patients (adjusted OR = 2.05, 95% CI = 1.02-4.13, P = .044). Our research suggested that four SNPs of LIN28A gene have a weak relationship with the risk of glioma in Chinese children. LIN28A rs3811463 TC/CC may increase the possibility of glioma in clinical stage III or stage III+IV patients which need larger samples and further confirmation.

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Association of TGFB1 gene polymorphisms with cervical cancer in Bangladeshi women: A case-control study

Tumor Biology ◽

10.3233/tub-200061 ◽

2021 ◽

Vol 43 (1) ◽

pp. 27-35

Author(s):

Mohd Nazmul Hasan Apu ◽

Most. Nasrin Aktar ◽

Md Morshadur Rahman ◽

Md Shaki Mostaid

Keyword(s):

Cervical Cancer ◽

Cancer Patients ◽

Transforming Growth Factor Beta ◽

Gene Polymorphisms ◽

Transforming Growth Factor ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Cervical Cancer Risk ◽

Pcr Rflp ◽

Tgfb1 Gene

OBJECTIVES: Genetic susceptibility to cervical cancer in relation to transforming growth factor beta 1 (TGFB1) gene polymorphisms has not been investigated extensively among the women in Bangladesh. So, the aim of this study was to find out the correlation of the polymorphisms of TGFB1 C509T (rs1800469) and T869C (rs1800470) with the risk of cervical cancer among the Bangladeshi women. STUDY DESIGN: 134 cervical cancer patients and 102 age-sex matched healthy controls were included from two institutions in Bangladesh. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for genotyping two TGFB1 single nucleotide polymorphisms C509T (rs1800469) and T869C (rs1800470) in patients and controls. RESULTS: No significant correlation was found between polymorphisms C509T (rs1800469) and T869C (rs1800470) of TGFB1 gene with cervical cancer in Bangladeshi women. In case of the cervical cancer patients who had first degree relatives with cancer were prone to carry the polymorphic version of the TGFB1 gene polymorphism at C509T (OR = 5.597, 95% CI = 1.224–25.597, p < 0.05) but may not result in the increase of developing cervical cancer. CONCLUSION: In summary, two polymorphisms C509T and T869C of TGFB1 gene may not be associated with cervical cancer risk in Bangladeshi women.

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The Functional Polymorphisms on Promoter Region of Matrix Metalloproteinase-12, -13 Genes May Alter the Risk of Epithelial Ovarian Carcinoma in Chinese

International Journal of Gynecological Cancer ◽

10.1111/igc.0b013e31819a1d8e ◽

2009 ◽

Vol 19 (1) ◽

pp. 129-133 ◽

Cited By ~ 16

Author(s):

Yan Li ◽

Jing-Hui Jia ◽

Shan Kang ◽

Xiao-Juan Zhang ◽

Jian Zhao ◽

...

Keyword(s):

Ovarian Carcinoma ◽

Promoter Region ◽

Malignant Tumors ◽

Chinese Women ◽

Clinical Stage ◽

Positive Association ◽

Epithelial Ovarian Carcinoma ◽

Nucleotide Polymorphisms ◽

Functional Polymorphisms ◽

Pathological Subtype

Backgrounds and Aims:Growing evidences indicate that single nucleotide polymorphisms (SNPs) of matrix metalloproteinases (MMPs) gene promoter may alter MMPs protein expression levels to influence malignant tumors developing and progressing. Our study was to assess the effects of the SNPs in the promoter region of MMP-12 and MMP-13 on the risk of epithelial ovarian carcinoma (EOC) developing and progressing.Methods:MMP-12 A-82G and MMP-13 A-77G SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism in 256 EOC patients and 329 controls.Results:The A/G genotype frequency of MMP-12 was significantly higher in patients than in controls (7.0% vs 3.3%, P = 0.04); similarly, the frequency of MMP-12 82G allele was higher in patients too (P = 0.04). Compared with A/A genotype, A/G genotype significantly increased the risk of EOC (odds ratio, 2.19; 95% confidence interval, 1.01-4.72). Age-stratified analysis showed that individuals with A/G genotype had a higher risk in the final diagnosis aged younger than 50 years. We observed no overall association between MMP-13-77A/G polymorphism and EOC. However, an elevated positive association was observed for A/A versus G/G + A/G genotypes in mucinous ovarian cancer. Combining the analyzed 2 SNPs, the haplotype distributions in patients were not significantly different from that in controls.Conclusion:These results suggested that the G allele of the MMP-12 82A/G polymorphism might be a risk factor for the development and progression of EOC and that the A/A genotype of MMP-13-77A/G polymorphism was associated with special pathological subtype and clinical stage in EOC at least in Chinese women.

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The association of integration patterns of human papilloma virus and single nucleotide polymorphisms on immune- or DNA repair-related genes in cervical cancer patients

Scientific Reports ◽

10.1038/s41598-019-49523-0 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Jungnam Joo ◽

Yosuke Omae ◽

Yuki Hitomi ◽

Boram Park ◽

Hye-Jin Shin ◽

...

Keyword(s):

Cervical Cancer ◽

Dna Repair ◽

Single Nucleotide Polymorphisms ◽

Cancer Patients ◽

Immune Cell ◽

Luciferase Assay ◽

Candidate Snps ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Chromosome 11P15

Abstract The present study investigated the association between single nucleotide polymorphisms (SNPs) in immune- or DNA repair-related genes and the integration pattern of human papillomavirus (HPV), a promising prognostic marker in cervical cancer. The HPV integration patterns of cervical cancer patients were determined by polymerase chain reaction and in situ hybridization, and categorized as episomal (group A), single-copy or multi-copy tandem repetition integrated (group B), and undetectable HPV types (group C). After sample and SNP quality control, 166,505 SNPs in 161 samples (38, 111, and 12 patients in groups A, B, and C, respectively) were examined. None of the SNPs reached genome-wide significance, and several candidate SNPs for future study were selected, including rs10999435 on chromosome 10q22, rs1322054 on chromosome 9q32-33, and rs10902171 on chromosome 11p15. Luciferase assay identified rs1322054 as the primary functional variant to regulate gene expression in immune cell. Further studies are needed to determine the genetic background of different integration patterns of HPV in cervical cancer patients.

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The Nonsynonymous Single-Nucleotide Polymorphisms in Codon 31 of p21 Gene and the Susceptibility to Cervical Cancer in Chinese Women

International Journal of Gynecological Cancer ◽

10.1111/igc.0b013e3181a8b950 ◽

2009 ◽

Vol 19 (6) ◽

pp. 1011-1014 ◽

Cited By ~ 9

Author(s):

Qifang Tian ◽

Weiguo Lu ◽

Huaizeng Chen ◽

Feng Ye ◽

Xing Xie

Keyword(s):

Cervical Cancer ◽

Single Nucleotide Polymorphisms ◽

Allele Frequency ◽

Chinese Women ◽

Host Susceptibility ◽

Genotype Distribution ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Increased Risk ◽

Significant Difference

Background:It was suggested that single-nucleotide polymorphisms in p21 codon 31 seem to be associated with a variety of human malignancies; very few studies have focused on the association between p21 codon 31 polymorphisms and cervical cancer. This study explored whether p21 codon 31 nonsynonymous single-nucleotide polymorphisms might be associated with an increased risk of cervical cancer development among Chinese women.Methods:Peripheral blood samples were obtained from patients with cervical cancer (n = 317) and healthy controls (n = 353) for detecting the biallelic polymorphisms at codon 31 of p21 gene by the mismatch amplification mutation assay-polymerase chain reaction. Cervix brush-off samples were obtained from patients with cervical squamous cell carcinoma (SCC) and controls for detection of high-risk human papillomavirus (HR-HPV).Results:The AGA (Arg) allele frequency in patients with cervical SCCs was significantly higher than that in controls. AGA/AGA and AGA/AGC genotypes were more frequently found in cervical SCCs than in controls. There was no significant difference of allele frequency or genotype distribution between cervical adenocarcinomas and controls, or between HR-HPV-positive and HR-HPV-negative groups.Conclusions:p21 Codon 31 with AGA (Arg) allele is a genetic risk factor of cervical SCC, and the increased risk is probably not caused by increasing host susceptibility to HR-HPV infection.

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Fas gene promoter –670 polymorphism in gynecological cancer

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200602001-00028 ◽

2006 ◽

Vol 16 (Suppl 1) ◽

pp. 179-182 ◽

Cited By ~ 2

Author(s):

M. Ueda ◽

Y. Terai ◽

K. Kanda ◽

M. Kanemura ◽

M. Takehara ◽

...

Keyword(s):

Ovarian Cancer ◽

Cervical Cancer ◽

Cancer Patients ◽

Germ Line ◽

Gynecological Cancer ◽

Gene Promoter ◽

Single Nucleotide ◽

Increased Risk ◽

Significant Difference ◽

Germ Line Polymorphism

Single-nucleotide polymorphism at −670 of Fas gene promoter (A/G) was examined in a total of 354 blood samples from normal healthy women and gynecological cancer patients. They consisted of 95 normal, 83 cervical, 108 endometrial, and 68 ovarian cancer cases. Eighty-three patients with cervical cancer had statistically higher frequency of GG genotype and G allele than 95 controls (P= 0.0353 and 0.0278, respectively). There was no significant difference in the genotype or allele prevalence between control subjects and endometrial or ovarian cancer patients. The Fas −670 GG genotype was associated with an increased risk for the development of cervical cancer (OR = 2.56, 95% CI = 1.08–6.10) compared with the AA genotype. The G allele also increased the risk of cervical cancer (OR = 1.60, 95% CI = 1.05–2.43) compared with the A allele. Germ-line polymorphism of Fas gene promoter −670 may be associated with the risk of cervical cancer in a Japanese population.

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miR-146a and miR-196a-2 genes polymorphisms and its circulating levels in lung cancer patients

The Journal of Biochemistry ◽

10.1093/jb/mvz044 ◽

2019 ◽

Vol 166 (4) ◽

pp. 323-329 ◽

Cited By ~ 2

Author(s):

Randa H Mohamed ◽

Heba F Pasha ◽

Doaa M Gad ◽

Mostafa M Toam

Keyword(s):

Lung Cancer ◽

Cancer Risk ◽

Cancer Patients ◽

Lung Cancer Risk ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Lung Cancer Patients ◽

Increased Risk ◽

Increase Risk ◽

Circulating Levels

AbstractRecently, MicroRNAs polymorphisms and their serum expression have been linked to increase risk of various cancers. The aim of this study was to elucidate the association between single nucleotide polymorphisms of miR-146a and miR-196a-2 and their serum expression and lung cancer risk. One hundred and twenty lung cancer patients and 120 health controls were included in this study. Genotyping and expression for miR-146a and miR-196a-2 were performed using polymerase chain reaction (PCR)-restriction fragment length polymorphism and quantitative real-time PCR. Individuals carrying miR-146a CG and CC genotypes had significantly increased risk for lung cancer than those carrying miR-146a GG genotype. MiR-146a expression significantly decreased in miR-146a CG and CC genotypes carriers as compared with GG genotype carriers. MiR-196a-2 CT and TT genotypes were significantly associated with increased lung cancer while the highest expression of MiR-196a-2 was detected in miR-196a-2 CC genotype carriers. Serum miR-146a was significantly decreased in lung cancer patients while serum miR-196a-2 expression was significantly increased in lung cancer patients. In conclusion, miR-146a and miR-196a-2 genes polymorphisms and their circulating levels were associated with lung cancer risk in Egyptians and may be helpful in early detection of lung cancer.

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Single nucleotide polymorphisms to predict taxanes toxicities and effectiveness in cancer patients

The Pharmacogenomics Journal ◽

10.1038/s41397-021-00227-7 ◽

2021 ◽

Author(s):

Sara Demurtas ◽

Nicla La Verde ◽

Selene Rota ◽

Giovanni Casazza ◽

Cristina Montrasio ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Cancer Patients ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Analysis of 4 Single-Nucleotide Polymorphisms in Relation to Cervical Dysplasia and Cancer Development Using a High-Throughput Ligation-Detection Reaction Procedure

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e31822b6299 ◽

2011 ◽

Vol 21 (9) ◽

pp. 1664-1671 ◽

Cited By ~ 12

Author(s):

Helmut von Keyserling ◽

Thomas Bergmann ◽

Miriam Schuetz ◽

Ursula Schiller ◽

Jonas Stanke ◽

...

Keyword(s):

Cervical Cancer ◽

Single Nucleotide Polymorphisms ◽

Genetic Markers ◽

Cervical Dysplasia ◽

Semantic Integration ◽

Cancer Development ◽

Large Sample Size ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Genetic Characteristics

BackgroundHost genetic characteristics and environmental factors may correlate with risk for cervical cancer development. Here we describe a retrospective screening study for single nucleotide polymorphisms (SNPs) in genetic markersTP53, MTHFR, CYP1A1,andCYP2E1in 749 patients.MethodsA multiplex ligation-dependent polymerase chain reaction approach was applied. We used archived material from human papillomavirus tests and correlated SNP genotypes to the corresponding clinical data. Semantic integration was used to identify and evaluate the clinical status from electronic health records.ResultsAn association with cervical cancer and high-grade dysplasia was found for the rare homozygous CC genotype (rs4646903) inCYP1A1(odds ratio [OR], 8.862). Odds ratios were also significantly elevated for heterozygousMTHFRCT genotype (rs1801133; OR, 1.457). No significant association was found inTP53(rs1042522) andCYP2E1(rs3813867). In addition, we found smokers at higher risk (OR, 2.688) and identified pregnancies as a significant risk factor (OR, 1.54).ConclusionsOur protocol enables a feasible way for further retrospective large sample size evaluation of potential genetic markers. This study revealed genetic associations of a rare SNP genotype with cervical dysplasia in one of the largest patient sample to date that warrants further investigation.

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