scholarly journals Inhibition ofStreptococcus gordoniiMetabolic Activity in Biofilm by Cranberry Juice High-Molecular-Weight Component

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Jegdish Babu ◽  
Cohen Blair ◽  
Shiloah Jacob ◽  
Ofek Itzhak
Keyword(s):  
Oral Cavity ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
Plaque Formation ◽  
High Molecular Mass ◽  
Cranberry Juice ◽  
Numerous Species ◽  
Low Concentrations ◽  
High Molecular Weight Component ◽  
Biofilm Development

Previous studies demonstrated that a cranberry high-molecular-mass, nondialyzable material (NDM) can inhibit adhesion of numerous species of bacteria and prevents bacterial coaggregation of bacterial pairs. Bacterial coaggregation leads to plaque formation leading to biofilm development on surfaces of oral cavity. In the present study, we evaluated the effect of low concentrations of NDM onStreptococcus gordoniimetabolic activity and biofilm formation on restorative dental surfaces. We found that the NDM selectively inhibited metabolic activity ofS. gordonii, without affecting bacterial viability. Inhibiting the metabolic activity of bacteria in biofilm may benefit the health of the oral cavity.

Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†

2020 ◽  
Vol 58 (7) ◽  
pp. 887-895 ◽  
Author(s):  
Judith Díaz-García ◽  
Maiken C Arendrup ◽  
Rafael Cantón ◽  
Julio García-Rodríguez ◽  
Ana Gómez ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Metabolic Activity ◽  
Crystal Violet ◽  
Biofilm Formation ◽  
Candida Spp ◽  
Biofilm Production ◽  
Hospital Environments ◽  
Marked Variability ◽  
Inert Surfaces ◽  
Biofilm Development

Abstract The capacity of Candida spp. to form biofilms allows them to attach either to living or inert surfaces, promoting their persistence in hospital environments. In a previous study, we reported strain-to-strain variations in Candida spp. biofilm development, suggesting that some genotypes may be greater biofilm formers than others. In this study, we hypothesize that isolates pertaining to clusters may be found more frequently in the environment due to their ability to form biofilms compared to singleton genotypes. Two hundred and thirty-nine Candida spp. isolates (78 clusters) from candidemia patients admitted to 16 hospitals located in different cities and countries—and the same number of singleton genotypes used as controls—were tested in terms of biofilm formation using the crystal violet and the XTT reduction assays. Candida albicans clusters showed higher biofilm formation in comparison to singleton genotypes (P < .01). The biofilms formed by intra-hospital C. albicans clusters showed higher metabolic activity (P < .05). Furthermore, marked variability was found among species and type of cluster. We observed that the higher the number of isolates, the higher the variability of biofilm production by isolates within the cluster, suggesting that the production of biofilm by isolates of the same genotype is quite diverse and does not depend on the type of cluster studied. In conclusion, candidemia Candida spp. clusters—particularly in the case of C. albicans—show significantly more biomass production and metabolic activity than singleton genotypes.

scholarly journals Murein Hydrolase LytF of Streptococcus sanguinis and the Ecological Consequences of Competence Development

2017 ◽  
Vol 83 (24) ◽  
Author(s):  
Nyssa Cullin ◽  
Sylvio Redanz ◽  
Kirsten J. Lampi ◽  
Justin Merritt ◽  
Jens Kreth
Keyword(s):  
Dental Caries ◽  
Oral Cavity ◽  
Cell Morphology ◽  
Biofilm Formation ◽  
Oral Bacteria ◽  
Tooth Surface ◽  
Content Type ◽  
Streptococcus Sanguinis ◽  
Murein Hydrolase ◽  
Biofilm Development

ABSTRACT The overall health of the oral cavity is dependent on proper homeostasis between health-associated bacterial colonizers and bacteria known to promote dental caries. Streptococcus sanguinis is a health-associated commensal organism, a known early colonizer of the acquired tooth pellicle, and is naturally competent. We have shown that LytF, a competence-controlled murein hydrolase, is capable of inducing the release of extracellular DNA (eDNA) from oral bacteria. Precipitated LytF and purified LytF were used as treatments against planktonic cultures and biofilms. Larger amounts of eDNA were released from cultures treated with protein samples containing LytF. Additionally, LytF could affect biofilm formation and cellular morphology. Biofilm formation was significantly decreased in the lytF-complemented strain, in which increased amounts of LytF are present. The same strain also exhibited cell morphology defects in both planktonic cultures and biofilms. Furthermore, the LytF cell morphology phenotype was reproducible in wild-type cells using purified LytF protein. In sum, our findings demonstrate that LytF can induce the release of eDNA from oral bacteria, and they suggest that, without proper regulation of LytF, cells display morphological abnormalities that contribute to biofilm malformation. In the context of the oral biofilm, LytF may play important roles as part of the competence and biofilm development programs, as well as increasing the availability of eDNA. IMPORTANCE Streptococcus sanguinis, a commensal organism in the oral cavity and one of the pioneer colonizers of the tooth surface, is associated with the overall health of the oral environment. Our laboratory showed previously that, under aerobic conditions, S. sanguinis can produce H2O2 to inhibit the growth of bacterial species that promote dental caries. This production of H2O2 by S. sanguinis also induces the release of eDNA, which is essential for proper biofilm formation. Under anaerobic conditions, S. sanguinis does not produce H2O2 but DNA is still released. Determining how S. sanguinis releases DNA is thus essential to understand biofilm formation in the oral cavity.

Efficacy of metal ions and isothiazolones in inhibiting Enterobacter cloacae BF-17 biofilm formation

2014 ◽  
Vol 60 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Gang Zhou ◽  
Long-jie Li ◽  
Qing-shan Shi ◽  
You-sheng Ouyang ◽  
Yi-ben Chen ◽  
...  
Keyword(s):  
Metal Ions ◽  
Biofilm Formation ◽  
High Capacity ◽  
Enterobacter Cloacae ◽  
High Concentration ◽  
Planktonic Growth ◽  
Low Concentrations ◽  
Negative Effect ◽  
Biofilm Development ◽  
Initial Biofilm

Enterobacter cloacae is a nosocomial pathogen. The E. cloacae strain BF-17, with a high capacity for biofilm formation, was screened and identified from industrially contaminated samples, carried out in our laboratory. To develop an efficient strategy to deal with biofilms, we investigated the effects of metal ions, including Na+, K+, Ca2+, Mg2+, Cu2+, and Mn2+, and 3 isothiazolones, on elimination of E. cloacae BF-17 biofilm formation by using a 0.1% crystal violet staining method. The results revealed that higher concentrations of Na+ or K+ significantly inhibited E. cloacae BF-17 biofilm development. Meanwhile, Ca2+ and Mn2+ stimulated biofilm formation at low concentration but exhibited a negative effect at high concentration. Moreover, biofilm formation decreased with increasing concentration of Mg2+ and Cu2+. The isothiazolones Kathon (14%), 1,2-benzisothiazolin-3-one (11%), and 2-methyl-4-isothiazolin-3-one (10%) stimulated initial biofilm formation but not planktonic growth at low concentrations and displayed inhibitory effects on both biofilm formation and planktonic growth at higher concentrations. Unfortunately, the 3 isothiazolones exerted negligible effects on preformed or fully mature biofilms. Our findings suggest that Na+, K+, Mg2+, and isothiazolones could be used to prevent and eliminate E. cloacae BF-17 biofilms.

scholarly journals Inhibition ofStreptococcus mutansBiofilm Formation byStreptococcus salivariusFruA

2011 ◽  
Vol 77 (5) ◽  
pp. 1572-1580 ◽  
Author(s):  
Ayako Ogawa ◽  
Soichi Furukawa ◽  
Shuhei Fujita ◽  
Jiro Mitobe ◽  
Taketo Kawarai ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Biofilm Formation ◽  
Sucrose Concentration ◽  
Gel Filtration ◽  
Microbial Interactions ◽  
Oral Disease ◽  
Mass Spectrometry Analysis ◽  
Oral Streptococci ◽  
Spectrometry Analysis ◽  
Biofilm Development

ABSTRACTThe oral microbial flora consists of many beneficial species of bacteria that are associated with a healthy condition and control the progression of oral disease. Cooperative interactions between oral streptococci and the pathogens play important roles in the development of dental biofilms in the oral cavity. To determine the roles of oral streptococci in multispecies biofilm development and the effects of the streptococci in biofilm formation, the active substances inhibitingStreptococcus mutansbiofilm formation were purified fromStreptococcus salivariusATCC 9759 and HT9R culture supernatants using ion exchange and gel filtration chromatography. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis was performed, and the results were compared to databases. TheS. salivariusHT9R genome sequence was determined and used to indentify candidate proteins for inhibition. The candidates inhibiting biofilms were identified asS. salivariusfructosyltransferase (FTF) and exo-beta-d-fructosidase (FruA). The activity of the inhibitors was elevated in the presence of sucrose, and the inhibitory effects were dependent on the sucrose concentration in the biofilm formation assay medium. Purified and commercial FruA fromAspergillus niger(31.6% identity and 59.6% similarity to the amino acid sequence of FruA fromS. salivariusHT9R) completely inhibitedS. mutansGS-5 biofilm formation on saliva-coated polystyrene and hydroxyapatite surfaces. Inhibition was induced by decreasing polysaccharide production, which is dependent on sucrose digestion rather than fructan digestion. The data indicate thatS. salivariusproduces large quantities of FruA and that FruA alone may play an important role in multispecies microbial interactions for sucrose-dependent biofilm formation in the oral cavity.

scholarly journals A lux/gfp dual label system for studying attachment and biofilm formation of Enterobacter sakazakii

SURG Journal ◽  
1969 ◽  
Vol 1 (1) ◽  
pp. 22-28 ◽  
Author(s):  
Melanie Thimodo
Keyword(s):  
Metabolic Activity ◽  
Biofilm Formation ◽  
Significant Feature ◽  
The Novel ◽  
Enterobacter Sakazakii ◽  
Reporter System ◽  
Cell Numbers ◽  
Gfp Reporter ◽  
Novel Method ◽  
Biofilm Development

A significant feature of Enterobacter sakazakii is the ability to form biofilms enabling its persistence in manufacturing and neonatal environments, and implicating it as the primary cause of outbreaks of neonatal meningitis. Conventional methods of studying E. sakazakii are time consuming, inaccurate and damaging to cells, preventing further analysis. A novel method for detecting biofilm formation has been utilized that takes advantage of a dual lux/gfp reporter system cloned into cells for simultaneous quantification of bacterial metabolic activity and cell numbers respectively. To evaluate the effectiveness and accuracy of the novel method compared to the conventional method, strains of bacteria were allowed to form biofilms and were measured using both methods. Biofilm formation of E. sakazakii strains over 2 days was measured and peak formation and changes in biofilm development was determined for each strain. The lux reporter was utilized to determine metabolic activity of cells in the biofilm, correlating to biofilm formation, over 3 days. Biofilm formation of strains was measured using both methods and compared to detect trends. This study found that the novel method was effective at detecting changes in metabolic activity of cells in biofilm. Cell numbers were to be simultaneously detected via the gfp reporter but filters with correct detection wavelength were unavailable thus cell numbers were not obtained here. These observations determine that this dual reporter system is a promising tool for monitoring bacteria in situ and for further understanding of biofilm formation.

scholarly journals Role of PvdQ in Pseudomonas aeruginosa virulence under iron-limiting conditions

Microbiology ◽  
2010 ◽  
Vol 156 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Pol Nadal Jimenez ◽  
Gudrun Koch ◽  
Evelina Papaioannou ◽  
Mariana Wahjudi ◽  
Joanna Krzeslak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Iron Homeostasis ◽  
Gene Deletion ◽  
Infection Model ◽  
Swarming Motility ◽  
Low Concentrations ◽  
Biofilm Development ◽  
Long Chain

PvdQ, an acylase from Pseudomonas aeruginosa PAO1, has been shown to have at least two functions. It can act as a quorum quencher due to its ability to degrade long-chain N-acylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL, leading to a decrease in virulence factors. In addition, PvdQ is involved in iron homeostasis by playing a role in the biosynthesis of pyoverdine, the major siderophore of P. aeruginosa. In accordance with earlier studies on RNA level, we could show at the protein level that PvdQ is only expressed when iron is present at very low concentrations. We therefore set out to investigate the two functions of PvdQ under iron-limiting conditions. Gene deletion of pvdQ does not affect growth of P. aeruginosa but abrogates pyoverdine production, and results in an accumulation of 3-oxo-C12-HSL. Phenotypic analyses of our ΔpvdQ mutant at low iron concentrations revealed that this mutant is impaired in swarming motility and biofilm formation. Additionally, a plant and a Caenorhabditis elegans infection model demonstrated that the deletion of pvdQ resulted in reduced virulence. None of the phenotypes in the present study could be linked to the presence or absence of AHLs. These results clearly indicate that under iron-limiting conditions PvdQ plays a major role in swarming motility, in biofilm development and in infection that is more likely to be linked to the pyoverdine pathway rather than the LasI/LasR/3-oxo-C12-HSL quorum-sensing circuit.

scholarly journals A streptococcal effector protein that inhibits Porphyromonas gingivalis biofilm development

Microbiology ◽  
2010 ◽  
Vol 156 (11) ◽  
pp. 3469-3477 ◽  
Author(s):  
Aaron B. Christopher ◽  
Annette Arndt ◽  
Carla Cugini ◽  
Mary E. Davey
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Developmental Process ◽  
Plaque Formation ◽  
Arginine Deiminase ◽  
Pcr Analysis ◽  
Effector Protein ◽  
Oral Streptococci ◽  
Genes Encoding ◽  
Biofilm Development

Dental plaque formation is a developmental process involving cooperation and competition within a diverse microbial community, approximately 70 % of which is composed of an array of streptococci during the early stages of supragingival plaque formation. In this study, 79 cell-free culture supernatants from a variety of oral streptococci were screened to identify extracellular compounds that inhibit biofilm formation by the oral anaerobe Porphyromonas gingivalis strain 381. The majority of the streptococcal supernatants (61 isolates) resulted in lysis of P. gingivalis cells, and some (17 isolates) had no effect on cell viability, growth or biofilm formation. One strain, however, produced a supernatant that abolished biofilm formation without affecting growth rate. Analysis of this activity led to the discovery that a 48 kDa protein was responsible for the inhibition. Protein sequence identification and enzyme activity assays identified the effector protein as an arginine deiminase. To identify the mechanism(s) by which this protein inhibits biofilm formation, we began by examining the expression levels of genes encoding fimbrial subunits; surface structures known to be involved in biofilm development. Quantitative RT-PCR analysis revealed that exposure of P. gingivalis cells to this protein for 1 h resulted in the downregulation of genes encoding proteins that are the major subunits of two distinct types of thin, single-stranded fimbriae (fimA and mfa1). Furthermore, this downregulation occurred in the absence of arginine deiminase enzymic activity. Hence, our data indicate that P. gingivalis can sense this extracellular protein, produced by an oral streptococcus (Streptococcus intermedius), and respond by downregulating expression of cell-surface appendages required for attachment and biofilm development.

Adhesion and Growth of Anaerobic Biofilms on Ion Exchange Resins

1986 ◽  
Vol 21 (4) ◽  
pp. 486-495 ◽  
Author(s):  
R.F.G. Selle Sardi ◽  
W. Bulani ◽  
W.L. Cairns ◽  
N. Kosaric
Keyword(s):  
Metabolic Activity ◽  
Cation Binding ◽  
Fatty Acid Substrate ◽  
Anaerobic Reactors ◽  
Initial Adhesion ◽  
Rapid Changes ◽  
Exchange Resins ◽  
Biofilm Development ◽  
First Time ◽  
Acid Substrate

Abstract Ion exchanger beads are explored as aids in accelerating the development of anaerobic biofilms for use in advanced anaerobic reactors. Initial adhesion and subsequent changes in adhesion and growth of anaerobic biofilms (as reflected in total supported biomass and metabolic activity) were monitored on different ion exchangers (strong cation, strong anion and weak anion) over a period of 12 days. Metabolic activity was recorded for the first time in this type of study using ATP biolumininescence assays which allowed monitoring of rapid changes in the biofilm development. Results indicate that the strong cation exchanger is a better overall substratum for anaerobic biofilm development due to its favorable property of dialent cation binding and adsorption of volatile fatty acid substrate for methanogens.

scholarly journals In VitroAnalyses of the Effects of Heparin and Parabens on Candida albicans Biofilms and Planktonic Cells

2011 ◽  
Vol 56 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Marisa H. Miceli ◽  
Stella M. Bernardo ◽  
T. S. Neil Ku ◽  
Carla Walraven ◽  
Samuel A. Lee
Keyword(s):  
Candida Albicans ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
High Dose ◽  
Dependent Manner ◽  
Planktonic Cells ◽  
Content Type ◽  
Heparin Sodium ◽  
The Individual

ABSTRACTInfections and thromboses are the most common complications associated with central venous catheters. Suggested strategies for prevention and management of these complications include the use of heparin-coated catheters, heparin locks, and antimicrobial lock therapy. However, the effects of heparin onCandida albicansbiofilms and planktonic cells have not been previously studied. Therefore, we sought to determine thein vitroeffect of a heparin sodium preparation (HP) on biofilms and planktonic cells ofC. albicans. Because HP contains two preservatives, methyl paraben (MP) and propyl paraben (PP), these compounds and heparin sodium without preservatives (Pure-H) were also tested individually. The metabolic activity of the mature biofilm after treatment was assessed using XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and microscopy. Pure-H, MP, and PP caused up to 75, 85, and 60% reductions of metabolic activity of the mature preformedC. albicansbiofilms, respectively. Maximal efficacy against the mature biofilm was observed with HP (up to 90%) compared to the individual compounds (P< 0.0001). Pure-H, MP, and PP each inhibitedC. albicansbiofilm formation up to 90%. A complete inhibition of biofilm formation was observed with HP at 5,000 U/ml and higher. When tested against planktonic cells, each compound inhibited growth in a dose-dependent manner. These data indicated that HP, MP, PP, and Pure-H havein vitroantifungal activity againstC. albicansmature biofilms, formation of biofilms, and planktonic cells. Investigation of high-dose heparin-based strategies (e.g., heparin locks) in combination with traditional antifungal agents for the treatment and/or prevention ofC. albicansbiofilms is warranted.

scholarly journals Sodium Salicylate Influences the Pseudomonas aeruginosa Biofilm Structure and Susceptibility Towards Silver

2021 ◽  
Vol 22 (3) ◽  
pp. 1060
Author(s):  
Erik Gerner ◽  
Sofia Almqvist ◽  
Peter Thomsen ◽  
Maria Werthén ◽  
Margarita Trobos
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Sodium Salicylate ◽  
Treatment Strategies ◽  
Cell Aggregation ◽  
Wound Fluid ◽  
Global Challenge ◽  
3D Collagen ◽  
Biofilm Development ◽  
Biofilm Structure

Hard-to-heal wounds are typically infected with biofilm-producing microorganisms, such as Pseudomonas aeruginosa, which strongly contribute to delayed healing. Due to the global challenge of antimicrobial resistance, alternative treatment strategies are needed. Here, we investigated whether inhibition of quorum sensing (QS) by sodium salicylate in different P. aeruginosa strains (QS-competent, QS-mutant, and chronic wound strains) influences biofilm formation and tolerance to silver. Biofilm formation was evaluated in simulated serum-containing wound fluid in the presence or absence of sodium salicylate (NaSa). Biofilms were established using a 3D collagen-based biofilm model, collagen coated glass, and the Calgary biofilm device. Furthermore, the susceptibility of 48-h-old biofilms formed by laboratory and clinical strains in the presence or absence of NaSa towards silver was evaluated by assessing cell viability. Biofilms formed in the presence of NaSa were more susceptible to silver and contained reduced levels of virulence factors associated with biofilm development than those formed in the absence of NaSa. Biofilm aggregates formed by the wild-type but not the QS mutant strain, were smaller and less heterogenous in size when grown in cultures with NaSa compared to control. These data suggest that NaSa, via a reduction of cell aggregation in biofilms, allows the antiseptic to become more readily available to cells.

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