scholarly journals Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
R. J. Mohd Salim ◽  
M. I. Adenan ◽  
A. Amid ◽  
M. H. Jauri ◽  
A. S. Sued
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Centella Asiatica ◽  
Ethyl Acetate Fraction ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Metal Chelating Activity ◽  
Extraction Parameters

The purpose of the study is to evaluate the relationship between the extraction parameters and the metal chelating activity of Centella asiatica (CA) and Erythroxylum cuneatum (EC). The response surface methodology was used to optimize the extraction parameters of methanolic extract of CA and EC with respect to the metal chelating activity. For CA, Run 17 gave optimum chelating activity with IC50 = 0.93 mg/mL at an extraction temperature of 25°C, speed of agitation at 200 rpm, ratio of plant material to solvent at 1 g : 45 mL and extraction time at 1.5 hour. As for EC, Run 13 with 60°C, 200 rpm, 1 g : 35 mL and 1 hour had metal chelating activity at IC50 = 0.3817 mg/mL. Both optimized extracts were further partitioned using a solvent system to evaluate the fraction responsible for the chelating activity of the plants. The hexane fraction of CA showed potential activity with chelating activity at IC50 = 0.090 and the ethyl acetate fraction of EC had IC50 = 0.120 mg/mL. The study showed that the response surface methodology helped to reduce the extraction time, temperature and agitation and subsequently improve the chelating activity of the plants in comparison to the conventional method.

scholarly journals OPTIMIZATION OF EXTRACTION PARAMETERS FOR TOTAL FLAVONOIDS FROM GARDENIA GUMMIFERA GUM RESIN BY RESPONSE SURFACE METHODOLOGY

2016 ◽  
Vol 8 (11) ◽  
pp. 64 ◽  
Author(s):  
Snehal B. Bhandare ◽  
Kirti S. Laddha
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Close Agreement ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Single Factor ◽  
Solvent Concentration ◽  
Extraction Parameters

Objective: To optimize the extraction parameters for determining the highest yield of Total Flavonoids from Gardenia gummifera gum resin.Methods: In the present study, response surface methodology (RSM) with three level Box Behenken design (BBD) was performed to optimize extraction parameters for total flavonoids. Solvent concentration(A), extraction time (B) and extraction temperature(C) were considered for single factor experiment.Results: The highest flavonoid concentration was obtained with acetone 45.00% v/v, time 101.46 min, temperature 41.57 °C. The average experimental TFC under optimal conditions was 161.14 mg/g which was in close agreement with the predicted value of 163.42 mg/g.Conclusion: RSM-BBD is successfully applied in optimizing extraction conditions for extraction of total flavonoids from G. gummifera gum resin.

scholarly journals OPTIMIZATION OF EXTRACTION PARAMETERS FOR FUCOXANTHIN, GALLIC ACID AND RUTIN FROM NITZSCHIA THERMALIS

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Vasfiye Hazal Özyur ◽  
Ayşegül Erdoğan ◽  
Zeliha Zeliha Demirel ◽  
Meltem Conk Dalay ◽  
Semih Ötleş
Keyword(s):  
Response Surface Methodology ◽  
Gallic Acid ◽  
Response Surface ◽  
Desirability Function ◽  
Antioxidant Properties ◽  
Value Added ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Extraction Parameters

Recently, microalgae have become important in their health, and cosmetic applications since they are viewed as new sources of carotenoids. Fucoxanthin is also a type of carotenoid. The anti-diabetic, anti-obesity, anti-cancer, and antioxidant properties of fucoxanthin have been widely reported. Since these valuable properties, they also represent a valuable resource of nutraceuticals for functional food applications. This study aims to determine the amount of fucoxanthin, gallic acid, and rutin in Nitzschia thermalis obtained from the Ege University Microalgae Culture Collection. The extraction parameters have been optimized using response surface methodology. The extraction temperature (25, 35, and 45°C), the extraction time (10, 20, and 30 min) and the biomass/solvent ratio (0.005, 0.001, and 0.015 g ml-1) have been assessed as response variables in the Box – Behnken design. The amount of fucoxanthin was determined by the C30 column at 450 nm, while both the amount of gallic acid and rutin were separated in the C18 column at 275 nm by HPLC-DAD. In the present study, the optimum extraction conditions providing the maximum amount of fucoxantin, gallic acid, and rutin were selected by applying the “desirability” function approach in response surface methodology. Finally, the temperature has been determined to be 27.30°C, the extraction time 10 minutes, and the biomass ratio 0.05 g ml-1. Under these conditions, the optimum fucoxanthin level has been determined as 5.8702 mg g-1, the gallic acid level as 0.0140 mg g-1, and the rutin level as 0.0496 mg g-1. The findings are in good agreement with international published values for fucoxanthin content. In addition, response surface methodology was shown to be an effective technique for optimising extraction conditions for maximum fucoxanthin yield. In conclusion, these findings may be applied in the development of extraction methodologies for value added microalgea products as well as can serve as a reference for the extraction of fucoxanthin having high gallic acid and rutin from other brown microalgae, and therefore it could potentially be applied in both pharmaceutical and food industries.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

scholarly journals Optimization of Supercritical Carbon Dioxide Extraction of Quercus infectoria Oil

2015 ◽  
Vol 74 (7) ◽  
Author(s):  
Liza Md Salleh ◽  
Stashia ELeaness Rosland Abel ◽  
Gholamreza Zahedi ◽  
Russly Abd Rahman ◽  
Hasmida Mohd Nasir ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Goodness Of Fit ◽  
Maximum Yield ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Predicted Values ◽  
Artificial Neural Network Ann ◽  
Quercus Infectoria

This current study focuses on the modelling and optimization of supercritical fluid extraction of Quercus infectoria galls oil. In this case, response surface methodology (RSM) and artificial neural network (ANN) were applied for the modelling and prediction of extraction yield of galls oil. A 17-run Box-Behnken Design (BBD) was employed to statistically optimize the process parameters of SC-CO2 extraction of Quercus infectoria galls at a condition as follows: pressure (5000, 6000, 7000 Psi), temperature (40, 50, 60°C) and extraction time (30, 45, 60 min). The maximum yield of the extracted oil is1.12 % and the optimum conditions are at an extraction pressure of 5574 Psi; extraction temperature of 75°C and extraction time of 54 min. Under the optimal conditions, the experimental results agree with the predicted values obtained through analysis of variance (ANOVA). This indicates a successful response surface methodology and highly satisfactory goodness of fit of the model used. The analysis of experimental design for process optimization results demonstrates that temperature and extraction time are the main parameters that influence the oil extraction of Quercus infectoria.

scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

scholarly journals Optimization of the extraction of natural phenolic antioxidants from the seeds of Tamarindus indica L. - an undervalued by product of food processing - using response surface methodology

2018 ◽  
Vol 62 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Atreyi Sarkar ◽  
Uma Ghosh
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Time ◽  
Phenolic Antioxidants ◽  
Extraction Temperature ◽  
Tamarindus Indica ◽  
Solvent Concentration ◽  
Antioxidant Power ◽  
Wide Range ◽  
Ferric Reducing Antioxidant Power

The seeds of Tamarindus indica are known to possess a wide range of phenolic compounds with high antioxidant activity as measured by the ferric reducing antioxidant power (FRAP). In the present study, the optimum conditions for the extraction of crude phenolic antioxidants from Tamarind seed were determined using response surface methodology (RSM). A central composite design (CCD) was used to investigate the effects of four independent variables, namely concentration of extractable solids in solvent (g/ml; X1), extraction time (h; X2), extraction temperature (°C; X3) and solvent concentration (%, v/v; X4) on the responses of total polyphenol content (TPC) and FRAP. The CCD consisted of 30 experimental runs. A second-order polynomial model was used for predicting the responses. Canonical analysis of the surface responses revealed that the predicted optimal conditions for the maximal yield of TPC and FRAP were concentration of extractable solids in solvent of 0.049 g/ml, extraction time of 3.24 h, extraction temperature of 45 °C and a solvent concentration of 50%. The experimental values in the optimised condition coincided with the predicted ones within a 95% confidence interval, hence indicating the suitability of the model and the success of RSM in optimizing the extraction parameters.

scholarly journals Box-Behnken Experimental Design for Extraction of Spinosin from Ziziphus mauritiana Lam. Seeds

2019 ◽  
Vol 31 (5) ◽  
pp. 1045-1048
Author(s):  
Quang Thuong Tran ◽  
Loan Thi Thanh Vu ◽  
Tram Huyen Le ◽  
Ly Thi Phuong Giang ◽  
Toyonobu Usuki
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Factorial Design ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Raw Material ◽  
Extraction Time ◽  
Ziziphus Mauritiana ◽  
Solid Ratio ◽  
Extraction Parameters

The objective of this study was to evaluate the effects of extraction parameters (ethanol concentration, extraction ratio of solvent to raw material and extraction time) on the yield of spinosin from the seeds of Ziziphus mauritiana Lam. Box-Behnken factorial design was used in association with response surface methodology. The optimal extraction conditions were an ethanol concentration of 61 %, 40 (v/w) liquid-to-solid ratio, and 2.6 h extraction time with reflux. Spinosin was purified using Diaion HP20SS as an adsorbent. The maximum spinosin yield was 1.18 mg/g raw material. Present findings can be applied to future approaches for maximizing the extraction of spinosin from Z. mauritiana Lam. seeds.

Optimization of Ultrasonic Extraction of Phenolics from Litchi Seed by Response Surface Methodology

2012 ◽  
Vol 610-613 ◽  
pp. 3387-3393
Author(s):  
Xiao Bing Huang ◽  
Li Jing Lin ◽  
Ji Hua Li ◽  
Xu Ran ◽  
Yong Fu Tang
Keyword(s):  
Response Surface Methodology ◽  
Regression Model ◽  
Response Surface ◽  
Maximum Yield ◽  
Methanol Concentration ◽  
Ultrasonic Extraction ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Quadratic Regression ◽  
Box Behnken Design

Optimization conditions for ultrasonic extraction of phenolics from litchi seed were studied using response surface methodology. A Box–Behnken design (BBD) was applied to determine the effects of extraction temperature, methanol concentration and extraction time on yield of phenolics. Then a quadratic regression model was developed and found to be statistically significant by examining its adequacy. According to the model, the maximum yield of phenolics was obtained at the theoretical extraction conditions described as follows: extraction temperature 90°C, methanol concentration 59% and extraction time 70 min. Under this condition, the experimental value was 5.48 ± 0.03% (n = 3) with gallic acid as the equivalent, which agreed with the predicted value (5.52%) closely.

scholarly journals OPTIMIZATION CONDITION EXTRACTION USED ENZYME WITH RESPONSE SURFACE METHODOLOGY TO EXTRACT ROSELLE PETALS (Hibiscus sabdariffa L.)

2019 ◽  
Vol 9 (2) ◽  
Author(s):  
Mardiah Mardiah ◽  
Rima Nidaul Hasanah ◽  
Noli Novidahlia ◽  
A.E. Zainal Hasan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Raw Material ◽  
Extraction Time ◽  
Anthocyanin Content ◽  
Extraction Temperature ◽  
Hibiscus Sabdariffa ◽  
Cellulase Enzymes ◽  
Yield Level

Roselle petals can be made into extracts as a source of raw material for food products and other products. Previous research states that the use of pectinase and cellulase enzymes can increase the yield including the anthocyanin content. This study aims to optimize the extraction conditions of roselle flower petals  (Hibiscus sabdariffa L.) using pectinase and cellulase enzymes by using Response Surface Methodology (RSM). The treatment conditions were using pectinase and cellulase enzyme (1: 1) with concentrations in the range between 500-1500 ppm, extraction temperature between 35-65 oC and extraction time between 30-90 minutes. Analysis of the best treatment was carried out on the yield level, anthocyanin content and total dissolved solids tested using central composite design, obtained R2 values for the three responses of 0.6. The results showed that based on the surface response, the optimum conditions of the best  extraction of the rosella at the addition of enzyme concentration of 1000 ppm, extraction temperature of 50 oC and extraction time of 10 minutes. In this condition, the yield of dried extracts of rosella was 84,20%, anthocyanins content 479,70 mg/L and total soluble solid 2,13 oBrix.

Sign in / Sign up

Export Citation Format

Share Document