scholarly journals Effect of Extraction Conditions on the Antioxidant Activity of Olive Wood Extracts

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Mercedes Pérez-Bonilla ◽  
Sofía Salido ◽  
Adolfo Sánchez ◽  
Teris A. van Beek ◽  
Joaquín Altarejos
Keyword(s):  
Geographical Origin ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Olive Tree ◽  
Extraction Yield ◽  
Scavenging Activity ◽  
Direct Extraction ◽  
Ethanolic Extracts ◽  
Wood Extracts ◽  
Main Components

An investigation to optimize the extraction yield and the radical scavenging activity from the agricultural by-product olive tree wood (Olea europaeaL., cultivar Picual) using six different extraction protocols was carried out. Four olive wood samples from different geographical origin, and harvesting time have been used for comparison purposes. Among the fifty olive wood extracts obtained in this study, the most active ones were those prepared with ethyl acetate, either through direct extraction or by successive liquid-liquid partitioning procedures, the main components being the secoiridoids oleuropein and ligustroside. An acid hydrolysis pretreatment of olive wood samples before extractions did not improve the results. In the course of this study, two compounds were isolated from the ethanolic extracts of olive wood collected during the olives’ harvesting season and identified as (7′′R)-7′′-ethoxyoleuropein (1) and (7′′S)-7′′-ethoxyoleuropein (2).

scholarly journals Radical Scavenging Activity Combination of Sambiloto (Andrographis paniculata Nees.) and Patikan Kebo (Euphorbia hirta L.) Ethanolic Extracts on 2,2-Diphenyl-1-Picrylhidrazyl (DPPH)

2018 ◽  
Vol 23 (3) ◽  
pp. 79
Author(s):  
Andayana Puspitasari Gani ◽  
Suwidjiyo Pramono ◽  
Sudibyo Martono ◽  
Sitarina Widyarini
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Andrographis Paniculata ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Euphorbia Hirta ◽  
Ic50 Value ◽  
Ethanolic Extracts ◽  
Dpph Radical Scavenging

Research of radical scavenging activity of sambiloto (Andrographis paniculata Nees) and patikan kebo (Euphorbia hirta L) ethanolic extracts and their combination toward 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) has conducted. Subjects of this experiment comprise sambiloto ethanolic extract, patikan kebo ethanolic extract, dechlorophyllated extract of sambiloto and combinations among those three. Dechlorophyllation by electrocoagulation method is done to reduce chlorophyll content thereby increasing the level of active compound in the sambiloto extract. The results of dechlorophyllation showed that only total flavonoid content in the extract of sambiloto increased, meanwhile the radical scavenging activity results showed that patikan kebo ethanolic extract has the lowest IC50 value (22,36 µg/mL) compared to sambiloto ethanolic extract (IC50 value 499,03 µg/mL). Extract combination may increase DPPH radical scavenging activity of the sambiloto extract (IC50 value 76,06 µg/mL) but not higher than that of patikan kebo single extract.

scholarly journals Free radical scavenging activity of ethanolic extracts from herbs and spices commercialized in Brazil

2008 ◽  
Vol 51 (6) ◽  
pp. 1225-1232 ◽  
Author(s):  
Lilian Regina Barros Mariutti ◽  
Gisela Pizarro de Mattos Barreto ◽  
Neura Bragagnolo ◽  
Adriana Zerlotti Mercadante
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Black Pepper ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Decay Rate Constant ◽  
Ethanolic Extracts ◽  
Radical Scavenging Properties ◽  
Herbs And Spices

Ethanolic extracts from 23 different dried herbs and spices commercialized in Brazil were investigated for their free radical scavenging properties using the stable free radicals 2,2'-diphenyl-β-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+), and Trolox as reference (TEAC) for both radicals. The kinetic curves for both radicals showed to follow the first order kinetics model and the decay rate constant (k obs) was calculated. For all the samples, the two methods showed a good linear TEAC correlation, indicating that the average reactivity of the compounds present in the ethanolic extracts was similar. Sage and rosemary extracts showed the highest free radical scavenging capacities, while onion showed negligible activity and colorifico, one of the most consumed spices in Brazil, showed low ABTS•+ scavenging activity. Three distinct situations were found for the extracts concerning the DPPH• scavenging capacities: (1) extracts, like rosemary and laurel, that presented the same efficient concentrations (EC50 ) but differed in the TEAC values and velocities of action (k obs), (2) extracts, such as garlic and basil, that showed similar EC50 and TEAC values, but different k obs values and (3) extracts that reacted at the same velocities but completely differed in the free radical scavenging capacities, like black pepper, savory, nutmeg, rosemary and sage. Similar considerations could be done for the ABTS•+ results. For the first time the ABTS•+ scavenging activity for allspice, basil, cardamom, chives, colorifico, cumin, dill, laurel, marjoram, parsley and tarragon was reported.

scholarly journals Antioxidative and Quality Properties of Full-Fat Date Seeds Brew as Influenced by the Roasting Conditions

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 226 ◽  
Author(s):  
Mohammad Fikry ◽  
Yus Aniza Yusof ◽  
Alhussein M. Al-Awaadh ◽  
Russly Abdul Rahman ◽  
Nyuk Ling Chin ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Browning Index ◽  
Phenolic Contents ◽  
Dpph Radical Scavenging

Full-fat roasted date seeds are considered an excellent source of antioxidants which can treat many diseases. The specific objectives were to investigate the effect of roasting temperature and time on the hardness of whole seeds, moisture content of the roasted date seeds powder, DPPH radical scavenging activity, total phenolic contents, extraction yield, pH, browning index and sensory properties of the brew prepared from the full-fat roasted date seeds and to construct descriptive models that could describe this effect. Date seeds were roasted at three temperatures (160, 180 and 200 °C) for different period of times (10, 20 and 30 min) using a natural conventional oven; then grinded and next brewed. Hardness of whole seeds, moisture content of the seeds powder, DPPH radical scavenging activity and total phenolic contents, extraction yield, pH and browning index and sensory properties of the brew were significantly affected by the roasting conditions. The statistical results indicated that the proposed model could adequately describe the measured properties. Strong correlations have been found among the properties of the brew as well. The producers of the date seeds brew can utilize these results for controlling the roasting process.

scholarly journals Composition and Antioxidant Activity of Essential Oils of Lippia Origanoides H.B.K. grown in Colombia

2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Elena Stashenko ◽  
Carlos Ruiz ◽  
Amner Muñoz ◽  
Martha Castañeda ◽  
Jairo Martínez
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
The Other ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Compound Identification ◽  
Microwave Assisted ◽  
Main Components

Microwave-assisted hydrodistillation, simultaneous distillation-solvent extraction, and supercritical fluid extraction, were used to isolate secondary metabolites from two Lippia origanoides chemotypes growing wild in Colombia. Compound identification was based on chromatographic and spectroscopic criteria. The main components identified in the essential oil of one chemotype were carvacrol (44.4 – 51.8%) and p-cymene (8.8 – 10.1%). Those of the other chemotype were p-cymene (11.3 – 15.7%) and 1,8-cineole (6.8 – 10.9%). The last one constitutes a new chemotype, characterized by its low thymol and carvacrol contents and very low antioxidant activity. The radical scavenging activity of the essential oil of the first chemotype was higher than that of butylated hydroxytoluene (BHT) and similar to that of α-tocopherol.

scholarly journals Comparative Study on Phytochemical Composition and In Vitro Radical Scavenging Activity of Ethanolic Extracts of Landolphia lanceolata and Nutraceutical-C24/7

2018 ◽  
Vol 10 ◽  
pp. 36-44
Author(s):  
Carole Nweze Chibuzo ◽  
Abdurrasheed Yusuf Hauwa ◽  
Ahmed Zubairu
Keyword(s):  
Vitamin C ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Vitamin B ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Fruit Extract ◽  
Phytochemical Composition ◽  
Ethanolic Extracts

This study investigated the phytochemical composition andin-vitroradical scavenging activity of ethanolic extracts of Nutraceutical-C24/7 andLandolphialanceolatafruit. Phytochemical screening ofLandolphialanceolatafruit extractindicated the presence of phenols, flavonoids, saponins, alkaloids, terpenoids, tannins, triterpenoids and steroids. While nutraceutical-C24/7 showed precence of with exception of saponins, terpenoids and triterpenoids. Some of the identified Phytochemicals and vitamins concentrations quantified were higher inLandolphialanceolatafruit extractthan nutraceutical-C24/7 with statistically significant difference (p<0.05) with the former having flavonoids 0.062±0.00mg/ml, phenol 0.097±0.00mg/ml, vitamin C 206±42mg/dL, vitamin B154.16±0.00mg/dL and vitamin B20.11±0.00mg/dL while, Nutraceutical-C24/7 extract had flavonoid 0.117±0.00mg/ml, phenol 0.032±0.00, vitamin C 102.24±0.00mg/dL, vitamin B123.36±0.00mg/dL, and vitamin B20.067±0.00mg/dL. The study also showed that at higher concentration, DPPH radical, ferric ion, and hydrogen peroxide the extracts radical scavenging activity increased. The study showed that the extracts scavenging activities at different concentrations had higher percentage inhibition on DPPH radical, reducing power of ferric ion capabilities and reducing composition of hydrogen peroxide indicated that ethanolic extract ofLandolphialanceolatafruits showed statistically significant different (p<0.05) when compared with ethanolic extract of Nutraceutical-C24/7. Standard vitamin C percentage inhibition for DPPH radical compared well with that ofLandolphialanceolatafruit extract at higher concentration with no statistical significant different (p>0.05).The indigenous functional foodLandolphialanceolatafruit can be concluded to be a good antioxidant that can scavenge, inhibit and quench free radicals. This suggests thatLandolphialanceolatamay be considered a good source and alternative antioxidant for developing countries like Nigeria.

scholarly journals Variation in Antioxidant Activity of Extracts of Acacia confusa of Different Ages

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Yu-Tang Tung ◽  
Shang-Tzen Chang
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Acacia Confusa ◽  
Ethanolic Extracts ◽  
Different Ages ◽  
Branch Wood

The antioxidant activity of extracts of Acacia confusa of different ages has been evaluated. Ethanolic extracts of bark, twigs, branches (2- and 5 cm-diameter branch-bark, 2- and 5 cm-diameter branch-wood), and leaves of 15-, 25-, and 35-year-old A. confusa were examined. Results indicate that the ethanolic extracts of 5 cm-diameter branch-bark and bark of A. confusa exhibited stronger antioxidant activities, and higher phenolic and proanthocyanidin contents than the other plant parts. Furthermore, twig, 2 cm-diameter branch-wood, 2 cm-diameter branch-bark, and 5 cm-diameter branch-wood extracts of 35-year-old specimens showed better antioxidant activities and higher phenolic contents, followed by the 25- and 15-year-old specimens tested. This reveals that the composition of extracts and their differential antioxidant activities are dependent on the age of A. confusa. In addition, the total phenolic content of A. confusa ethanolic extracts has a high positive correlation with the DPPH free-radical scavenging activity (R2 = 0.83), superoxide radical-scavenging activity (R2 = 0.66), ferrous ion-chelating effect (R2 = 0.67), and reducing power (R2 = 0.92).

scholarly journals Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 319 ◽  
Author(s):  
Anita Smailagić ◽  
Petar Ristivojević ◽  
Ivica Dimkić ◽  
Tamara Pavlović ◽  
Dragana Dabić Zagorac ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Prunus Avium ◽  
Black Locust ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Principal Component ◽  
Antimicrobial Activities ◽  
Scavenging Activity ◽  
Data Set ◽  
Wood Extracts

The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak (Quercus petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.), mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), and wild cherry (Prunus avium L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed different chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or caffeic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: Quercus spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against Staphylococcus aureus for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.

scholarly journals ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

2017 ◽  
Vol 9 (2) ◽  
pp. 279 ◽  
Author(s):  
Ahmed R. Abu-raghif ◽  
Ghaith A. Jasim ◽  
Muneer M. Hanoon
Keyword(s):  
Antioxidant Activity ◽  
Cell Line ◽  
Proliferative Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Extraction Yield ◽  
Gas Chromatography Mass Spectrometry ◽  
Scavenging Activity

<p><strong>Objective: </strong>The aim of this study was to investigate the capability of <em>Zizyphus spina christi</em> methanol extract to inhibit cancer cell line proliferation.</p><p><strong>Methods: </strong>The leaves of <em>Zizyphus spina christi</em> were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. <strong></strong></p><p><strong>Results: </strong>The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC<sub>50</sub> was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC<sub>50</sub> of 33.91 mg/ml.</p><p><strong>Conclusion: </strong>Methanol extract showed<strong> </strong>potential anti-proliferative activity against RD cell line,<strong> </strong>which could be due to its antioxidant activity.</p>

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