scholarly journals Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 319 ◽  
Author(s):  
Anita Smailagić ◽  
Petar Ristivojević ◽  
Ivica Dimkić ◽  
Tamara Pavlović ◽  
Dragana Dabić Zagorac ◽  
...  

The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak (Quercus petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.), mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), and wild cherry (Prunus avium L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed different chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or caffeic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: Quercus spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against Staphylococcus aureus for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Mercedes Pérez-Bonilla ◽  
Sofía Salido ◽  
Adolfo Sánchez ◽  
Teris A. van Beek ◽  
Joaquín Altarejos

An investigation to optimize the extraction yield and the radical scavenging activity from the agricultural by-product olive tree wood (Olea europaeaL., cultivar Picual) using six different extraction protocols was carried out. Four olive wood samples from different geographical origin, and harvesting time have been used for comparison purposes. Among the fifty olive wood extracts obtained in this study, the most active ones were those prepared with ethyl acetate, either through direct extraction or by successive liquid-liquid partitioning procedures, the main components being the secoiridoids oleuropein and ligustroside. An acid hydrolysis pretreatment of olive wood samples before extractions did not improve the results. In the course of this study, two compounds were isolated from the ethanolic extracts of olive wood collected during the olives’ harvesting season and identified as (7′′R)-7′′-ethoxyoleuropein (1) and (7′′S)-7′′-ethoxyoleuropein (2).


2021 ◽  
Vol 45 (2) ◽  
pp. 223-231
Author(s):  
Kara Guzel ◽  
Mahmut Ulger ◽  
Ahmet Kahraman

The phytochemical contents of Salvia virgata mericarps and the antioxidant and antimicrobial activities of its ethanol extract were studied for the first time. S. virgata mericarps were collected in the province of Trabzon, Turkey. Chemical analyses were performed using chromatographic methods. The total phenolic and flavonoid contents and antioxidant potential were measured using Folin-Ciocalteu, Al(NO3)3, and DPPH radical scavenging activity methods, respectively. The antimicrobial activity was evaluated using the microdilution method for all the tested bacterial and fungal strains, with the exception of Mycobacterium tuberculosis for which the resazurin microtiter plate method was applied. The mericarps were rich in glutamic acid (3934 mg/100 g), potassium (12578.8 ?g/g), calcium (12092.0 ?g/g), and dietary fibers (35.565 g/100 g). The total phenolic and flavonoid contents were 2.50 ?g GAE/mg extract and 0.34 ?g QE/mg extract, respectively. The most effective DPPH free radical scavenging activity determined for the highest applied concentration was 92.44%. The ethanol extract obtained from the mericarps was found to be as effective as the reference drug ampicillin (MIC value = 125 ?g/mL) against the nosocomial pathogen Acinetobacter baumannii. In conclusion, S. virgata mericarps provide good nutritional value with low amounts of carbohydrates and high dietary fibers, amino acids, minerals and total phenolic and flavonoid contents and medicinal properties.


2010 ◽  
Vol 5 (9) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ismiarni Komala ◽  
Takuya Ito ◽  
Yasuyuki Yagi ◽  
Fumihiro Nagashima ◽  
Yoshinori Asakawa

Crude extracts of the Tahitian liverworts Mastigophora Diclados and Frullania sp., the Indonesian Frullania sp., Dumortiera hirsuta and Marchantia sp., and the Japanese Porella perrottetiana were investigated chemically by using gas chromatography-mass spectrometry (GC-MS). All extracts contained various volatile sesqui- and diterpenoids and a few aromatic compounds. The Tahitian M. diclados and Frullania sp., and the Indonesian Frullania sp. exhibited cytotoxic activity against HL-60 and KB cell lines. The extracts of the Tahitian M. diclados and the Indonesian Marchantia sp. showed radical scavenging activity, whereas the crude extracts of the Tahitian M. diclados and Frullania sp., and the Indonesian Frullania and Marchantia sp. showed antimicrobial activity against Staphylococcus aureus and Bacillus subtilis.


2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Jaiprakash Sharanappa Biradar ◽  
Parveen Rajesab ◽  
Naveen Jaiprakash Biradar ◽  
Sasidhar Balappa Somappa

A new series of novel triazolothiadiazepinylindole analogues were synthesized with an aim to examine possible antioxidant and antimicrobial activities. The titled compounds (3a–z) were obtained in good yield by reacting 5-(5-substituted-3-phenyl-1H-indol-2-yl)-4-amino-4H-1,2,4-triazole-3-thiols1a–cwith 3-(2,5-disubstituted-1H-indol-3-yl)-1(4-substituted phenyl)prop-2-en-1-ones2a–i. All the newly synthesized compounds were characterized by IR,1H NMR, mass spectroscopic and analytical data. The synthesized analogues were tested for antioxidant and antimicrobial potency. Among the tested compounds3a–cand3j–lhave shown very promising free radical scavenging activity and total antioxidant capacity. Compounds3d–f,3m–o, and3s–zhave shown excellent ferric reducing antioxidant activity. An outstanding antimicrobial activity is observed with compounds3a–cand3j–l.


Crystals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 989
Author(s):  
Adnan Shahzad ◽  
Ezzat Khan ◽  
Muhammad Said ◽  
Gul Shazada Khan ◽  
Mian Gul Syed ◽  
...  

The reaction of 1,3-Diisobutyl thiourea (Tu) with metal salts, {[CuX (X = Cl, I)], [ZnCl2] and [HgI2] in an appropriate stoichiometric ratio afforded the corresponding metal complexes [Tu2CuCl] (1), [Tu3CuI] (2), [Tu2ZnCl2] (3) and [Tu2HgI2] (4) in good yields. The FT-IR data show typically broad signals (3278–3288 cm−1) attributed to the involvement of NH bonds in extensive hydrogen bonding. The structures of complexes were proposed based on a spectroscopic data set. Compounds 1 and 2 were additionally characterized by single-crystal X-ray analysis. Complexes 1–4 were tested for their free radical scavenging efficiency using 2,2-diphenyl-1-picrylhydrazyl free radical (hereafter abbreviated as DPPH). The free radical scavenging activity was a function of decrease in the resultant absorption of DPPH solution after the mixing of an appropriate concentration of the respective complex. The activity of complexes was determined to be dose dependent and increased concentration of the complex resulted in improved antioxidant activity. Compound 1 was found to be the most efficient, with 79.9% free radical scavenging activity. Complexes were also tested for their efficiency against selected strains of bacteria (E. coli, S. flexneri, S. typhi, and P. aeruginosa) and the activities were compared to commercially available standard drug cephradine. Compound 1 was more active against P.aeruginosa (ZI 13.25), while compound 4 was found to be more active against E. coli (ZI 11.0), S. flexneri (ZI 11.2), and S. typhi (ZI 10.5).


2011 ◽  
Vol 29 (No. 6) ◽  
pp. 575-585 ◽  
Author(s):  
J.M. Čanadanović-Brunet ◽  
S.S. Savatović ◽  
G.S. Ćetković ◽  
J.J. Vulić ◽  
S.M. Djilas ◽  
...  

We described the in vitro antioxidant and antimicrobial activities of ethanol, acetone, and water extracts of beet root pomace. Total contents of phenolics (316.30&ndash;564.50 mg GAE/g of dry extract), flavonoids (316.30&ndash;564.50&nbsp;mg RE/g of dry extract), betacyanins (18.78&ndash;24.18 mg/g of dry extract), and betaxanthins (11.19&ndash;22.90 mg/g of dry extract) after solid-phase extraction were determined spectrophotometrically. The antioxidant activity was determined by measuring the reducing power and DPPH scavenging activity by spectrometric metod, and hydroxyl and superoxide anion radical scavenging activity by ESR spectroscopy. In general, the reducing power of all the beet root pomace extracts increased with increasing concentrations. The DPPH-free radical scavenging activity of the extracts, expressed as EC<sub>50</sub>, ranged from 0.133 mg/ml to 0.275 mg/ml. Significant correlation was observed between all phytochemical components and scavenging activity. 0.5 mg/ml of ethanol extract completely eliminated hydroxyl radical, which had been generated in Fenton system, while the same concentration of this extract scavenged 75% of superoxide anion radicals. In antibacterial tests, Staphylococcus aureus and Bacillus cereus showed higher susceptibility than Escherichia coli and Pseudomonas aeruginosa.


Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1125-1134
Author(s):  
A.A. Adan ◽  
R.A. Ojwang ◽  
E.K. Muge ◽  
B.K. Mwanza ◽  
E.N. Nyaboga

The processing of jackfruit (Artocarpus heterophyllus Lam) yields a considerable amount of bio-waste. Accumulation of this waste is considered a health risk because it is a potential source of air and water pollution. Recycling of the unutilized fruit parts, therefore, reduces the quantity and the impact of the bio-waste released to the environment. The purpose of this study was to determine the phytochemical profile, antioxidant and antimicrobial activities of extracts from three fruit parts (peel, fiber and the core) of jackfruit sampled from the coastal region of Kenya. Different extraction techniques and solvents were tested. The highest phenolic and flavonoid content of the peels, fiber and the core were obtained from methanol extracts following a 48-hour incubation. The values were recorded at 17.07±5.16 mg/g, 23.28±4.73 mg/g, and 15.68±3.74 mg/g for the phenolics and 28.55±12.42 mg/g, 35.4±9.53 mg/g and 36.23±2.54 mg/g for the flavonoids, respectively. The highest tannin content was obtained from distilled water extracts following homogenization recorded at 10.82±2.63 mg/g, 10.39±4.10 mg/g and 10.52±1.05 mg/g for peels, fiber and core, respectively. The fiber extracts gave the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity followed by the core at 61.51±29.90% and 51.06±33.39%, respectively. The antioxidant activity was highest for methanol fiber extracts at 61.51±29.90% for DPPH radical scavenging activity and 7.94±4.56 mg/mL for reducing power assay. The best antibacterial activity against Xanthomonas axonopodis pv. manihotis (Xam) was obtained from Ethyl acetate extracts showed. The unutilized jackfruit parts, therefore, are a potential source of natural antioxidants as well as antibacterial, for agriculture and food industry


2021 ◽  
pp. 166-174
Author(s):  
Arif Nur Ikhsan ◽  
Abdul Rohman ◽  
Anggita Rosiana Putri ◽  
Fella Syifa ◽  
Mabrurotul Mustafidah ◽  
...  

Patin fish (Pangasius micronemus), Gabus fish (Channa striata) and Bandeng fish (Chanos chanos) are the freshwater fish that are widely cultivated in Indonesia, fish oils extracted are believed has antioxidant activity. The goals of this study was (1) determining antioxidant activities using radical scavenging activity (RSA) 2,2’-diphenyl-1-picrylhydrazil (DPPH), (2) classifying of fish oil from different species and extraction method using principal component analysis (PCA) and cluster analysis (CA) and predicting antioxidant activities of fish oil from different species and extraction method using FTIR spectroscopy combined principal component regression (PCR) and partial least square regression (PLS). The results exhibit radical scavenging activity (RSA) of patin fish flesh oil (maceration dried samples using chloroform) giving highest RSA (23.57 ± 0.14). PCA and CA succesfully classify of fish oil from different species and extraction based on PC1 - PC2 score plots and grouping into 4 groups based on dendogram. Optimal PCR method were obtained from second derivative spectra at wavenumbers region 1440 – 1741 cm-1 with highest R2 (0,9794), lowest RMSEC (0,737), and lowest RMSEP (0,927) in calibration and validation models. From this study we can concluded FTIR spectroscopy combined with chemometrics can be classifying and predicting antioxidant activity of fish oil from different species and extraction method successfully.


Fitoterapia ◽  
2005 ◽  
Vol 76 (3-4) ◽  
pp. 348-351 ◽  
Author(s):  
Joaquín Altarejos ◽  
Sofía Salido ◽  
Mercedes Pérez-Bonilla ◽  
Pablo J. Linares-Palomino ◽  
Teris A. van Beek ◽  
...  

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