scholarly journals Biogenic Synthesis of Silver Nanoparticles Using Scenedesmus abundans and Evaluation of Their Antibacterial Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Nafe Aziz ◽  
Tasneem Fatma ◽  
Ajit Varma ◽  
Ram Prasad
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Visible Spectrum ◽  
Potential Candidate ◽  
Gradual Change ◽  
Antibacterial Efficacy ◽  
Silver Particles ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Uv Visible

Silver nanoparticle (AgNP) was synthesized using the cell free extract of Scenedesmus abundans with AgNO3. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), and Photoluminescence. Bioreduction of Ag+ ions showed a gradual change in the colour of the extract and nanoparticles were synthesized having the range of 420–440 nm under UV-visible spectrum. The antibacterial efficacy was assessed against pathogenic bacteria E. coli, Klebsiella pneumoniae, and Aeromonas hydrophila. The present study revealed that the AgNPs prepared from Scenedesmus abundans show antibacterial efficacy against the test pathogens. The bioaccumulation of silver particles makes the organism potential candidate for ecofriendly silver biorecovery system and S. abundans can be used as a source of silver nanoparticles.

Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

2012 ◽  
Vol 5 (1) ◽  
pp. 1638-1642 ◽  
Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Human Beings ◽  
Streptomyces Species ◽  
Force Microscopy ◽  
E Coli ◽  
Extracellular Synthesis ◽  
Atomic Force ◽  
Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

2019 ◽  
Vol 12 (5) ◽  
pp. 4644-4647
Author(s):  
K.K. Gupta ◽  
Neha Kumari ◽  
Neha Sinha ◽  
Akruti Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Bacterial Species ◽  
Antimicrobial Property ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Light Yellow ◽  
Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

scholarly journals Biosynthesis of DLLAE blended silver nanoparticles and their response against periodontitis triggering bacteria

2020 ◽  
Vol 11 (2) ◽  
pp. 1849-1856
Author(s):  
Chin Zi Hang ◽  
Neeraj Kumar Fuloria ◽  
Oh Jian Hong ◽  
Chuah Bee Kim ◽  
Bernice Yii Shu Ting ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Stability Study ◽  
E Coli ◽  
Strong Signal ◽  
Antimicrobial Potential ◽  
Optimization Study ◽  
Uv Visible ◽  
Cubic Structure ◽  
Inhibitory Potential

Facts over microorganisms to predominate periodontitis, shifting of human microbiota by Dimocarpus longan (D. longan) plant, and potentiation of antimicrobial activity by biosynthetic silver nanoparticles (SNPs) intended present study to biosynthesize, optimize, characterize and evaluate the antimicrobial potential of silver nanoparticles (SNPs) obtained using D. longan leaves aqueous extract (DLLAE). Study involved preparation of DLLAE using decoction method. The DLLAE was subjected to biosynthesis of SNPs followed by optimization (using UV-Visible spectrometry), characterization (by FTIR, FESEM, XRD, and EDX), stability, and antimicrobial activity of SNPs against periodontitis triggering human microflora. Biosynthesized SNPs exhibited signal between 416-453 nm. Optimization study established AgNO3 concentration (5 mM), pH 4, DLLAE and AgNO3 ratio (1:9) and temperature (60°C) as parametric requirement for SNPs biosynthesis using DLLAE. Stability study exhibited signal between 489-553 nm supporting SNPs stability. Characterization data of FESEM showed that SNPs were poly dispersed, and spherical shaped. Biosynthesized SNPs size ranged from 74.82 nm to 131.5 nm. The XRD data revealed presence of signals at 38.08°, 44.33°, 64.47°, and 78.83° 2θ values indexed to silver cubic structure planes. In EDX study, silver exhibited strong signal (55.54%). Antimicrobial investigation explored the high inhibitory potential of SNPs against B. subtilis and P. aeruginosa; and low inhibitory potential against S. aureus and E. coli. Present study conclude that biosynthesis of SNPs using DLLAE is an efficient method and biosynthetic SNPs possess high antimicrobial potential against P. aeruginosa and B. subtilis the periodontitis triggering pathogens.

scholarly journals Synthesis of silver nanoparticles: double-green approach of using chitosan and microwave technique towards antimicrobial activity against pathogenic bacteria

2020 ◽  
Vol 10 (4) ◽  
pp. 5918-5922 ◽  
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Size Range ◽  
Low Cost ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Heterogeneous Distribution ◽  
E Coli ◽  
Green Approach ◽  
Dual Mechanism

A double green approach to the synthesis of silver nanoparticles using chitosan as a reducing agent and microwave radiation has been carried out in this study. Chitosan was extracted from marine crab carapace and the morphology of the produced chitosan was characterized using Scanning Electron Microscope (SEM). The production of silver nanoparticles (AgNPs) was monitored by the formation of surface plasmon resonance (SPR) at the λmax= 420 nm, indicates the reduction of the oxidation state of Ag+ to Ag0. The XRD data confirms the crystallinity nature of obtained AgNps, while the SEM and TEM revealed the formation of near-spherical shapes, polydisperse, heterogeneous distribution of AgNps with the size range between 7 to 25 nm. The antimicrobial activities of the AgNPs were investigated using The prepared AgNPs demonstrated dual mechanism action with chitosan, which acted as a stabilizer on the surface of AgNPs, and enhance the inhibition zone against E. tarda and E. coli. Therefore, the synthesized AgNPs in this study may have excellent potential for clinical application as it is green, low-cost and eco-friendly.

Comparative Analysis of Antibiotic Effect of AgNO3 and Silver Nanoparticles Synthesized from Penicillium fellutanum

2013 ◽  
Vol 760 ◽  
pp. 9-14 ◽  
Author(s):  
Anima Nanda ◽  
Shahnaz Majeed
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Culture Filtrate ◽  
Pathogenic Bacteria ◽  
Room Temperature ◽  
High Dose ◽  
Fungal Culture ◽  
Proteus Vulgaris ◽  
E Coli ◽  
Antibiotic Effect

The proposed study was to synthesize silver nanoparticles by using filamentous fungus Penicillium fellutanum. From the ancient period, silver has been used against microorganisms due to its own antibacterial properties. The fungal culture of Penicillium fellutanum was isolated from the soil samples of Sathyabama University, Chennai, Tamil Nadu, India. The fungal isolates were inoculated in culture medium and incubated at room temperature for three days and the culture filtrate was separated and divided into two parts. One part of the culture filtrate was mixed with equal volume of 1mM silver nitrate [AgNO3,(1 mM)] and agitated at room temperature in dark condition. And the second half was kept as such. In the other hand only 1mM silver nitrate was added into the equal volume of Milli Q water and one half was plain Milli Q water. All the four samples were tested against pathogens like Bacillus cereus, E. coli, Proteus vulgaris, Staphylococcus aureus, Staphylococcus epidermidis and the results were compared. Proteus vulgaris showed the highest zone where E coli showed the least zone of inhibition, in the culture filtrate added with silver nitrate. The cell filtrate on treatment with silver nitrate and prior incubation were observed for change in color and characterized by UV-Vis spectrophotometer which detected AgNPs in the solution. The maximum absorbance 410nm confirmed the formation of silver nanoparticles. Size and morphology of silver nanoparticle were investigated using Atomic Force microscopy (AFM). The silver Nanoparticles after confirmation were checked for its antibacterial activity against selected pathogenic bacteria. The biologically synthesized Nanoparticles from Penicillium fellutanum showed the good inhibitory effect against the selected pathogens, which would be the novel remedy substituent in the place of high dose antibiotics.

scholarly journals Bioremoval of PVP coated silver nanoparticles using Aspergillus niger: the role of exopolysaccharides

2021 ◽  
Author(s):  
Ola M. Gomaa ◽  
Amar Alrshim ◽  
Anindya Chanda
Keyword(s):  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Wastewater Treatment Plants ◽  
Aqueous Media ◽  
Visible Spectrum ◽  
Ag Nps ◽  
Characteristic Peak ◽  
Uv Visible

Abstract The present work aims to study the removal of Polyvinylpyrrolidone coated silver nanoparticles (PVP-Ag-NPs) using Aspergillus niger and depict the role of exopolysaccharides in the removal process. Our results show that the majority of PVP-Ag-NPs were attached to fungal pellets. About 74% and 88% PVP-Ag-NPs were removed when incubated with A. niger pellets and exopolysaccharide-induced A. niger pellets, respectively. Ionized Ag decreased by 553 and 1290 fold under the same conditions as compared to stock PVP-Ag-NP. PVP-Ag-PVP resulted in an increase in reactive oxygen species (ROS) in 24h. The UV-Visible spectrum shows the disappearance of Ag characteristic peak and the broadness of the spectrum suggested an increase in size. Dynamic Light Scattering results showed an increase in PVP-Ag-NPs size from 28.4 nm to 115.9 nm for A. niger pellets and 160.3 nm after removal by stress-induced A. niger pellets and further increased to 650.1 nm for in vitro EPS removal. Our findings show that EPS can be used for nanoparticle removal, by increasing the net size of nanoparticles in aqueous media, this will, in turn, facilitate its filtration through conventional filtration techniques commonly used at wastewater treatment plants.

scholarly journals Synthesis, Characterization and Antitumor Efficacy of Silver Nanoparticle from Agaricus bisporus Pileus, Basidiomycota

2018 ◽  
Vol 17 (2) ◽  
pp. 75-87
Author(s):  
Mustafa Nadhim OWAID ◽  
Ghassan Adnan NAEEM ◽  
Rasim Farraj MUSLIM ◽  
Raheel Saeed OLEIWI
Keyword(s):  
Silver Nanoparticles ◽  
Uv Radiation ◽  
Agaricus Bisporus ◽  
Visible Spectrum ◽  
Silver Ions ◽  
Mouse Cell ◽  
Face Centered Cubic ◽  
Uv Visible ◽  
Face Centered ◽  
Biosynthesized Agnps

The object of this study is to synthesize and characterize silver nanoparticles from Agaricus bisporus pileus extracts and their applications. Agaricus bisporus-mediated synthesis of AgNPs was characterized using changing the color solution, UV-Visible spectroscopy, SEM, AFM, SPM, FTIR spectrum, XRD, and EDS analyses. The change of the mixture color of 10-3 M AgNO3 with the watery extract of fresh A. bisporus caps from colorless to brown color is an indicator for the formation of silver nanoparticles (AgNPs). The UV-Visible spectrum exhibits the absorption peak at 418 nm. The FTIR spectra exhibited that the structures of amino acids, polysaccharides, and polyphenols in the crude extract of A. bisporus are not affected because of the joining and interaction of their functional groups with silver ions, and act as reducing and capping agents to the biosynthesized Ag nanoparticles. SEM and EDS refer to the formation of AgNPs with irregular or spherical shapes. The XRD pattern exhibits face-centered cubic (fcc) silver nanocrystals, with crystalline AgNPs size of 43.9 nm. The biosynthesized AgNPs play a suitable role against mouse cell line, which has receptors for polioviruses (L20B). After exposure of the colloid AgNPs to UV radiation (256 nm), the absorption band transferred from 418 nm to 435 nm, indicating that UV rays affect on physical properties of AgNPs. Roughness average of the biosynthesized AgNPs from A. bisporus caps is 15.4 nm, but the roughness is increased after UV irradiation for 1 h to average 33.6 nm. Histograms of particle size distribution of AgNPs show the average of AgNPs is 103.57 nm, while the size of nanoparticles reaches 69.47 nm after exposure to UV radiation of 256 nm. The use of UV radiation leads to enhanced characteristics of silver nanoparticles.

Biosynthesis of Silver Nanoparticles from Eucalyptus corymbia Leaf Extract at Optimized Conditions

2019 ◽  
Vol 25 ◽  
pp. 32-45 ◽  
Author(s):  
Munyao Joshua Sila ◽  
Michira Immaculate Nyambura ◽  
Deborah Atieno Abong’o ◽  
Francis B. Mwaura ◽  
Emmanuel Iwuoha
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Narrow Range ◽  
Visible Spectrum ◽  
Extraction Temperature ◽  
Transmission Electron ◽  
Uv Visible ◽  
Silver Nitrate Concentration ◽  
The Fourier Transform ◽  
20 Nm

This study reports the biosynthesis of narrow range diameter silver nanoparticles at optimum conditions usingEucalyptus corymbiaas a reducing and stabilizing agent. Optimal conditions for biosynthesis of silver nanoparticles (AgNPs) were found to be; an extraction temperature of 90°C, pH of 5.7 a Silver Nitrate concentration of 1mM and AgNO3to plant extract ratio of 4:1. UV-Visible spectroscopy monitored the formation of colloidal AgNPs. The UV-Visible spectrum showed a peak around 425 nm corresponding to the Plasmon absorbance of the AgNPs. The size and shape characterization of the AgNPs was done using Transmission Electron Microscopy (TEM) techniques which revealed narrow range diameter (18-20 nm), almost monodispersed AgNPs, spherical in nature and with minimal agglomeration. Energy Dispersive X-ray (EDX) results showed the presence of two peaks at 3.0 and 3.15 keV in the silver region. The Fourier Transform Infrared-Spectra (FTIR) of the plant extract and the AgNPs gave rise to vibrational peaks at 3260 and 1634 wavenumbers which are due to the presence of OH and –C=C-functional groups respectively.

scholarly journals Conductivity, Surface Tension, and Comparative Antibacterial Efficacy Study of Different Brands of Soaps of Nepal

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Narendra Kumar Chaudhary ◽  
Arush Bhattarai ◽  
Biswash Guragain ◽  
Ajaya Bhattarai
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Efficacy Study ◽  
Antibacterial Efficacy ◽  
Solution Properties ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
E Coli ◽  
Diffusion Technique ◽  
Bioactive Ingredients

The current study aims to evaluate the solution properties and antibacterial efficacy study of five different brands of toilet soaps of Nepal such as Okhati (OKT), Lifebuoy (LFBY), Lux (LX), Liril (LRL), and Chiuree Neem (NM). The evaluation of critical micelle concentration (CMC) and their thermodynamics and surface properties are also reported. This study was further extended to evaluate antibacterial efficacy against three pathogenic bacteria such as Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Proteus vulgaris (P. vulgaris) by disc diffusion technique, and it was done at four different concentrations of soap. The bioactive ingredients present in them provide antibacterial potency to cure various skin problems caused by bacterial pathogens. Similarly, the antibacterial potency of LFBY was found higher than other soaps. Based on these studies, we can simply take LFBY soap at the highest rank in regards to antibacterial sensitivity.

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