scholarly journals Synthesis of silver nanoparticles: double-green approach of using chitosan and microwave technique towards antimicrobial activity against pathogenic bacteria

2020 ◽  
Vol 10 (4) ◽  
pp. 5918-5922 ◽  
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Size Range ◽  
Low Cost ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Heterogeneous Distribution ◽  
E Coli ◽  
Green Approach ◽  
Dual Mechanism

A double green approach to the synthesis of silver nanoparticles using chitosan as a reducing agent and microwave radiation has been carried out in this study. Chitosan was extracted from marine crab carapace and the morphology of the produced chitosan was characterized using Scanning Electron Microscope (SEM). The production of silver nanoparticles (AgNPs) was monitored by the formation of surface plasmon resonance (SPR) at the λmax= 420 nm, indicates the reduction of the oxidation state of Ag+ to Ag0. The XRD data confirms the crystallinity nature of obtained AgNps, while the SEM and TEM revealed the formation of near-spherical shapes, polydisperse, heterogeneous distribution of AgNps with the size range between 7 to 25 nm. The antimicrobial activities of the AgNPs were investigated using The prepared AgNPs demonstrated dual mechanism action with chitosan, which acted as a stabilizer on the surface of AgNPs, and enhance the inhibition zone against E. tarda and E. coli. Therefore, the synthesized AgNPs in this study may have excellent potential for clinical application as it is green, low-cost and eco-friendly.

scholarly journals EVALUATION OF PHYTOCHEMICAL CONSTITUENTS AND ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICLE SYNTHESIZED IPOMOEA NIL AGAINST SELECTED PATHOGENS

2017 ◽  
Vol 10 (3) ◽  
pp. 183
Author(s):  
Asha Sivaji ◽  
Asha A ◽  
Rajeshkumar S
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Pathogenic Bacteria ◽  
Color Change ◽  
Silver Ions ◽  
Inhibition Zone ◽  
E Coli ◽  
Ipomoea Nil ◽  
Inhibition Zones ◽  
Phytochemical Constituents

ABSTRACTObjective: The objective of this study is to investigate a simple and feasible approach for the production of silver nanoparticles (AgNPs) by using leafextract of Ipmoea nil and to evaluate its antibacterial activity.Methods: The AgNPs synthesized was characterized by a change in color and the absorption peaks by ultraviolet-visible spectroscopy. The phytocompounds responsible for the reduction and capping of silver ions was known from Fourier transform infrared spectra and phytochemical analysis.The antibacterial effects of prepared aqueous AgNPs were detected against five types of pathogenic bacteria, including Gram-negative and Grampositivebacteria (Escherichia coli, Salmonella, Bacillius, Proteus, and Klebsilla pneumonia) using agar well diffusion method.Results: A peak absorption value between 400 and 450 nm and the color change of the extract from yellowish to red wine were corresponds tothe plasmon absorbance of AgNPs. On the other hand, aqueous extract of I. nil mediated AgNPs found to be effective against tested microorganisms(Salmonella, Bacillus, and Proteus) with inhibition zone in the range of 10-13 mm (20 μl and 10 μl) except E. coli and K. pneumonia. Furthermore,aqueous extract of I. nil leaves had no ability to suppress the growth of the tested microorganisms in the concentration of 10 μl. The control alsoproduced similar inhibition zones like AgNPs.Conclusions: Our findings indicated that green synthesized AgNPs mediated by I. nil leaf extract had an efficient anti-bactericidal activity against thebacterial species tested. Hence, further studies are needed to highlight its mechanism and application as an antibacterial agent.Keywords: Ipomoea nil, Silver nanoparticles, Antibacterial activity, Bacteria.

Antibacterial Effects of Long-Chain Polyphosphates on Selected Spoilage and Pathogenic Bacteria

2008 ◽  
Vol 71 (7) ◽  
pp. 1401-1405 ◽  
Author(s):  
JEREMY A. OBRITSCH ◽  
DOJIN RYU ◽  
LUCINA E. LAMPILA ◽  
LLOYD B. BULLERMAN
Keyword(s):  
Food Industry ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Lag Phase ◽  
E Coli ◽  
Inhibition Of Growth ◽  
K 12 ◽  
Chain Lengths ◽  
And Staphylococcus Aureus ◽  
Long Chain

The antimicrobial activities of four long-chain food-grade polyphosphates were studied at concentrations allowed in the food industry (<5,000 ppm) in defined basal media by determining the inhibition of growth of three gram-negative and four gram-positive spoilage and pathogenic bacteria. Both generation time and lag phase of Escherichia coli K-12, E. coli O157: H7, and Salmonella Typhimurium were increased with all of the polyphosphates tested. Bacillus subtilis and Staphylococcus aureus were more sensitive to polyphosphates, but not in all cases, with multiphased growth. The growth of Lactobacillus plantarum was inhibited by polyphosphates at concentrations above 750 ppm, but the lag time of Listeria monocytogenes was shortened by the presence of polyphosphates. No single polyphosphate was maximally inhibitory against all bacteria. Polyphosphates with chain lengths of 12 to 15 were significantly different from those with chain lengths of 18 to 21 depending on the organism and concentrations of polyphosphate used. Overall, higher polyphosphate concentrations resulted in greater inhibition of bacterial growth.

scholarly journals Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa Wallich) as Antibacterial Agent

2018 ◽  
Vol 10 (3) ◽  
pp. 546-552
Author(s):  
Maria Yasinta Moi ◽  
Endang Kusdiyantini ◽  
Sri Pujiyanto
Keyword(s):  
Antibacterial Agent ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Biochemical Properties ◽  
Rrna Gene ◽  
Plant Seed ◽  
E Coli ◽  
Antibiotic Compounds ◽  
Screening Result

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.

Nanocomposite cellulose fabrics with in situ generated silver nanoparticles by bioreduction method

2020 ◽  
pp. 152808372092473 ◽  
Author(s):  
Suchart Siengchin ◽  
Pawinee Boonyasopon ◽  
Vajja Sadanand ◽  
Anumakonda Varada Rajulu
Keyword(s):  
Silver Nanoparticles ◽  
Size Range ◽  
Low Cost ◽  
Aqueous Dispersion ◽  
X Ray Diffraction ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Bed Materials ◽  
Cellulose Fabrics

In the present work, nanocomposite cellulose fabrics with in situ generated silver nanoparticles were prepared by bioreduction method employing aqueous dispersion of low-cost natural turmeric powder as a reducing agent and different concentrated aqueous AgNO3 as source solutions. The prepared nanocomposite cellulose fabrics were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and antibacterial tests. The nanocomposite cellulose fabrics had roughly spherical silver nanoparticles in the size range of 41–130 nm with an overall average of 78 nm. The X-ray analysis indicated the generation of both silver nanoparticles and Ag2O nanoparticles in the nanocomposite cellulose fabrics. The nanocomposite cellulose fabrics retained the generated AgNPs even after repeated detergent washings. The prepared nanocomposite cellulose fabrics exhibited excellent antibacterial activity against both the Gram-negative and Gram-positive bacteria and hence can be considered as antibacterial hospital-bed materials, apparels, etc.

scholarly journals Synthesis of Plant-Mediated Silver Nanoparticles UsingDioscorea batatasRhizome Extract and Evaluation of Their Antimicrobial Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
P. C. Nagajyothi ◽  
K. D. Lee
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Room Temperature ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Synthesize Silver Nanoparticles ◽  
Dioscorea Batatas

The eco-friendly synthesis of nanoparticles through various biological means helps to explore various plants for their ability to synthesize silver nanoparticles (AgNPs). Here we have synthesized AgNPs by using rhizome extract ofDioscorea batatasat as well as room temperature (). AgNPs were characterized under UV-vis spectrophotometer, SEM, FTIR, XRD, and EDX. The antimicrobial activity of AgNPs was evaluated on gram positive (B. substilisandS. aureus), gram negative (E. coli), and fungi (S. cerivisaeandC. albicans). At room temperature,S. cerivisaeandC. albicanswere found to be more susceptible to AgNPs than at .

scholarly journals A Green Approach to Synthesize Silver Nanoparticles in Starch-co-Poly(acrylamide) Hydrogels by Tridax procumbens Leaf Extract and Their Antibacterial Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Siraj Shaik ◽  
Madhusudana Rao Kummara ◽  
Sudhakar Poluru ◽  
Chandrababu Allu ◽  
Jaffer Mohiddin Gooty ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Silver Ions ◽  
Inhibition Zone ◽  
Zone Method ◽  
Green Approach ◽  
Tridax Procumbens ◽  
Poly Acrylamide

A series of starch-co-poly(acrylamide) (starch-co-PAAm) hydrogels were synthesized by employing free radical redox polymerization. A novel green approach, Tridax procumbens (TD) leaf extract, was used for reduction of silver ions (Ag+) into silver nanoparticles in the starch-co-PAAm hydrogel network. The formation of silver nanoparticles was confirmed by UV-visible spectroscopy (UV-Vis), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (X-RD) studies. 22% of weight loss difference between hydrogel and silver nanocomposite hydrogel (SNCH) clearly indicates the formation of silver nanoparticles by TGA. TEM images indicate the successful incorporation of silver nanoparticles ranging from 5 to 10 nm in size and spherical in shape with a narrow size distribution. These developed SNCHs were used to study the antibacterial activity by inhibition zone method against gram-positive and gram-negative bacteria such as Bacillus and Escherichia coli. The results indicated that these SNCHs can be used potentially for biomedical applications.

scholarly journals BIOSYNTHESIS OF SILVER NANOPARTICLES USING MARINE MICROALGAE ISOCHRYSIS sp.

2017 ◽  
Vol 2 (1) ◽  
pp. 1-12
Author(s):  
Tevan R ◽  
Saravanan Jayakumar ◽  
Nor Haledah Ahmad Sahimi ◽  
Nur Farah Ain Iqbal ◽  
Iffah Zapri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metal Nanoparticles ◽  
Low Cost ◽  
Spherical Shape ◽  
Antimicrobial Activities ◽  
Marine Microalgae ◽  
X Ray ◽  
Potential Applications ◽  
Uv Visible ◽  
193 Nm

Biosynthesis of metal nanoparticles has received a remarkable attention due to their eco-friendly and potential applications in pharmaceutical and medical fields. The searches for natural alternatives to replace biosynthetic nanoparticles have resulted in extensive studies of microalgal derived metal nanoparticles. Since there are very limited reports on Isochrysis sp. in synthesising metal nanoparticles, a novel initiative was taken to induce an environmentally friendly and low cost technique to biosynthesise the silver nanoparticles (AgNPs) using marine microalgae, Isochrysis sp. Further, the synthesised silver nanoparticles were screened against human pathogen for antimicrobial effects. The characterisation of nanoparticles were confirmed by UV visible spectroscopy, field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRD). The results obtained from characterisations indicate that the AgNPs have an almost spherical shape with a various size of 98.1 to 193 nm. The synthesised nanoparticles exhibited outstanding antioxidant and antimicrobial activities.

scholarly journals Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Michele Sumampouw
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Potato Dextrose Agar ◽  
Rhizophora Stylosa ◽  
E Coli

Abstract: The aim of this study is to investigate the antibacterial effect of endophytic fungi originated from the root of mangrove Rhizophora stylosa growing on Mangrove Plantation Area around DAS Simpang 5 Jl. Piere Tendean Manado. The method of this research was using combination of PDA and NA media. Two species of endophytic fungi were extracted from the root of R. stylosa, black mycelium fungi as isolate A and brown mycelium fungi as isolate B. The activities of both isolates were tested against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The result showed the same inhibition zone of isolate A and B to S. aureus, while, isolate A showed bigger the inhibition zone in comparison to isolate B against E. coli. As a conclusion, the endophytic fungi taken from the root of R. stylosa have antibacterial activity toward S. aureus and E. coli. Keywords: endophytic fungi, Rhizophora stylosa, antibacterial, Staphylococcus aureus, Escherichia coli.   Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar bakau Rhizophora stylosa yang ditanam di sekitar Perairan Daerah Aliran Sungai Simpang Lima Jl. Piere Tendean Manado. Metode penelitian dilakukan dengan menggunakan kombinasi media Potato Dextrose Agar (PDA) dan Nutrient Agar (NA). Dari akar bakau R. stylosa diperoleh dua  jenis jamur endofit yaitu isolat A dengan karateristik miselium jamur berwarna hitam dan isolat B yang dengan karakteristik miselium berwarna coklat. Kedua isolat ini selanjutnya diujikan aktivitasnya terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan isolat A dan B memberikan  zona hambat yang sama terhadap bakteri Staphylococcus aureus dan pada bakteri Escherichia coli isolat A menunjukkan diameter zona hambat yang lebih besar dibandingkan isolat B. Kesimpulan, Jamur endofit yang diisolasi dari akar bakau R. Stylosa memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan E. coli. Kata kunci: jamur endofit, Rhizophopra stylosa, antibakteri, Staphylococcus aureus, Escherichia coli.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS Leucetta chagosensis DARI PERAIRAN PULAU MANTEHAGE SULAWESI UTARA TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 834
Author(s):  
Eunike Pelealu ◽  
Defny S. Wewengkang ◽  
Surya Sumantri Abdullah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Disk Diffusion ◽  
E Coli ◽  
Activity Test ◽  
Escherichia Coli Bacteria ◽  
Metabolite Content

ABSTRACTSponges are one of the biota components that make up coral reefs which are quite widely distributed. The metabolite content in the sponge can ward off and inhibit the pathogenic bacteria that interfere with it. This study aims to determine the activity of inhibiting bacterial growth from the extract and fraction of Leucetta chagosensis sponge against the growth of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The samples were extracted using the maceration method with 95% ethanol solvent and then fractionated using 3 solvents with different polarity levels, namely methanol, n-hexane and chloroform. Activity test using the disk diffusion agar method of Kirby and Bauer. Only the MeOH fraction was able to inhibit the growth of E. coli bacteria with an average inhibition zone of 6.88 mm. Whereas for S.aureus bacteria extracts and all fractions showed activity to inhibit bacterial growth with an average inhibition zone of EtOH (6.61 mm), CHCI3 (6.68 mm), n-hexane (7.83 mm) and MeOH (8.00 mm), respectively. All activities that are shown are categorized as weak (weak).Keywords: Antibacterial, Leucetta chagosensis, Staphylococcus aureus, Escherichia coli ABSTRAKSpons merupakan salah satu komponen biota penyusun terumbu karang yang penyebarannya cukup luas. Kandungan metabolit yang ada di dalam spons dapat menangkal dan menghambat bakteri patogen pengganggunya.  Penelitian ini bertujuan untuk melihat aktivitas menghambat pertumbuhan bakteri dari ekstrak dan fraksi spons Leucetta chagosensis. terhadap pertumbuhan bakteri Gram positif Staphylococcus aureus dan Gram negatif Escherichia coli. Sampel di ekstraksi menggunakan metode maserasi dengan pelarut etanol 95% lalu di fraksinasi dengan menggunakan 3 pelarut dengan tingkat kepolaran yang berbeda yaitu metanol, n-heksan dan kloroform. Uji aktivitas menggunakan metode disk diffusion agar Kirby dan Bauer.  Hanya fraksi MeOH yang mampu menghambat pertumbuhan bakteri E.coli dengan zona hambat rata-rata 6,88 mm. Sedangkan terhadap bakteri S.aureus ekstrak dan semua fraksi menunjukan aktifitas menghambat pertumbuhan bakteri dengan rata-rata zona hambat masing-masing EtOH (6,61 mm), CHCl3 (6,68 mm), n-Heksan (7,83 mm), dan MeOH (8,00 mm). Semua aktivitas yang ditunjukan dikategorikan lemah (weak).Kata kunci : Antibakteri, Leucetta chagosensis, Staphylococcus aureus,  Escherichia coli

scholarly journals Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli. (Antibacterial Test of Chitosan Nanoparticles against Staphylococcus Aureus and Escherichia coli)

2020 ◽  
Vol 10 (1) ◽  
pp. 7
Author(s):  
Alce Magani ◽  
Trina Tallei ◽  
Beivy Kolondam
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Chitosan Nanoparticles ◽  
Inhibition Zone ◽  
Antibacterial Test ◽  
E Coli ◽  
Inhibition Of Growth ◽  
Significant Difference ◽  
One Way Anova

Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli.(Antibacterial Test of Chitosan Nanoparticles against Staphylococcus Aureus and Escherichia coli) Alce K. Magani*, Trina E. Tallei, Beivy J. KolondamProgram Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] (Article History: Received 30-12-2019; Revised 15-01-2020; Accepted 23-01-2020) Abstrak Antibakteri merupakan zat yang dapat menghambat pertumbuhan bakteri dan dapat membunuh bakteri penyebab infeksi. Staphylococcus aureus dan Escherichia coli merupakan bakteri Gram positif dan Gram negatif yang dapat menimbulkan infeksi atau penyakit dalam tubuh. Penelitian ini bertujuan untuk menguji aktivitas bakteri patogen dengan memakai nanopartikel kitosan sebagai antibakteri yang dibuat dalam empat konsentrasi (0,5%, 1%, 1,5% dan 2%) serta penggunaan kontrol asam asetat 1%, ciprofloxacin dan air steril sebagai pembanding. Metode penelitian yang digunakan yaitu metode gelasi ionik untuk pembuatan nanopartikel kitosan dan difusi agar untuk pengujian antibakteri. Data dianalisis dengan One Way Anova yang dilanjutkan dengan metode BNT (Beda Nyata Terkecil). Hasil penelitian diperoleh penghambatan pertumbuhan bakteri S. aureus dan E. coli tertinggi pada konsentrasi 0,5%, dengan diameter zona hambat hari pertama sampai hari ketiga 12,31 mm, 9,98 mm, dan 20,46 mm pada S. aureus dan 15,88 mm, 18,71 mm, dan 20,43 mm pada E. coli, kategori kuat, dan bersifat bakteriostatik dan penghambatan terendah pada konsentrasi 2% dengan diameter zona hambat pada S. aureus yaitu 5,56 mm, 5,50 mm, dan 5,40 mm, dan pada E. coli yaitu 5,93 mm, 9,64 mm, dan 12,58 mm, kategori sedang, dan bersifat bakteriostatik. Kata kunci: Kitosan, nanopartikel kitosan, aktivitas antibakteri.  Abstract Antibacteria is a substance that can inhibit the growth of bacteria and able to kill bacteria that cause infections. Staphylococcus aureus and Escherichia coli are Gram positive and Gram negative bacteria that able to cause infections or diseases. This study aimed to examine the activity of pathogenic bacteria by using chitosan nanoparticles as antibacterial. The treatments were made in four concentrations (0.5%, 1%, 1.5% and 2%) and, for comparison, there were also acetic acid control, ciprofloxacin and sterile water. The research method used is the ionic gelation method for the manufacture of chitosan nanoparticles and agar diffusion for antibacterial testing. Data were analyzed with One Way Anova followed by LSD (Least Significant Difference) method. The results showed the highest inhibition of growth of S. aureus and E. coli bacteria at a concentration of 0.5%, with a diameter of inhibition zones of the first day to the third day of 12.31 mm, 9.98 mm, and 20.46 mm in S. aureus and 15,88 mm, 18,71 mm, and 20,43 mm in E. coli, the strong category, and are bacteriostatic and the lowest inhibition was at 2% concentration with inhibition zone diameters in S. aureus namely 5.568 mm, 5.50 mm, and 5, 40 mm, and in E. coli, 5.93 mm, 9.63 mm and 12.58 mm, the medium category and bacteriostatic.Key words: Chitosan, nanoparticles chitosan, antibacterial activity.

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