scholarly journals Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Marcelle Lins de Albuquerque de Carvalho ◽  
Daniele Fernandes Carvalho ◽  
Edelvio de Barros Gomes ◽  
Roberto Nobuyuki Maeda ◽  
Lidia Maria Melo Santa Anna ◽  
...  
Keyword(s):  
Culture Media ◽  
Low Cost ◽  
Cellulase Production ◽  
Aeration Rate ◽  
Penicillium Funiculosum ◽  
Stirred Tank Bioreactor ◽  
Operational Conditions ◽  
Cellulose Breakdown ◽  
Full Factorial ◽  
Second Generation Ethanol

Increasing interest in the production of second-generation ethanol necessitates the low-cost production of enzymes from the cellulolytic complex (endoglucanases, exoglucanases, and β-glucosidases), which act synergistically in cellulose breakdown. The present work aimed to optimise a bioprocess to produce these biocatalysts from the fungus Penicillium funiculosum ATCC11797. A statistical full factorial design (FFD) was employed to determine the optimal conditions for cellulase production. The optimal composition of culture media using Avicel (10 g·L−1) as carbon source was determined to include urea (1.2 g·L−1), yeast extract (1.0 g·L−1), KH2PO4 (6.0 g·L−1), and MgSO4·7H2O (1.2 g·L−1). The growth process was performed in batches in a bioreactor. Using a different FFD strategy, the optimised bioreactor operational conditions of an agitation speed of 220 rpm and aeration rate of 0.6 vvm allowed the obtainment of an enzyme pool with activities of 508 U·L−1 for FPase, 9,204 U·L−1 for endoglucanase, and 2,395 U·L−1 for β-glucosidase. The sequential optimisation strategy was effective and afforded increased cellulase production in the order from 3.6 to 9.5 times higher than production using nonoptimised conditions.

Nitrogen and Phosphorus Removal and Biomass Production by the Green Microalgae in Piggery Wastewater

2012 ◽  
Vol 599 ◽  
pp. 608-613
Author(s):  
Jen Jeng Chen ◽  
Yu Ru Li ◽  
Meei Fang Shue ◽  
Li Ho Tseng ◽  
Wen Liang Lai
Keyword(s):  
Biomass Production ◽  
Phosphorus Removal ◽  
Low Cost ◽  
Biomass Productivity ◽  
Aeration Rate ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Experimental Conditions ◽  
Piggery Wastewater ◽  
Full Factorial

Use of microalgae to remove inorganic nutrients from wastewater and their great potential for low-cost biomass production is gaining attraction. The effect of piggery wastewater content, aeration rate, cultivation temperature, and light intensity on nitrogen and phosphorus removal and biomass production were studied by using a Box-Behnken experimental design under full factorial methodology. Under experimental conditions considered cultures with aeration increased the ammonium and orthophosphate removal efficiency up to an average of 65.3±17 % and 51±7.2 %, respectively and an increase of biomass productivity ranging from 20.8±11 mg/L.d to 52.3±5.5 mg/L.d. The aeration rate was the most important factor influencing the nutrients removal and biomass production.

scholarly journals Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk

2019 ◽  
Vol 21 (1) ◽  
pp. 82-90
Author(s):  
Valentina Palacio-Castañeda ◽  
Alejandra Pérez-Hoyos ◽  
Daniel Carrascal-Correa ◽  
Victor Manuel Osorio-Echeverri
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Media ◽  
Human Cancer ◽  
Aeration Rate ◽  
Pigment Production ◽  
Stirred Tank ◽  
Human Cancer Cell ◽  
Stirred Tank Bioreactor ◽  
Human Cancer Cell Lines ◽  
Yield Production

Prodigiosin,  a  pigment  produced  by Serratia  marcescensinhibits  the  growth  of  different  microorganisms  and  the  proliferation  of some human cancer cell lines. Prodigiosin is usually produced by fermentations of substrates such as starch and proteins, andpig-ment yield depends on the concentration of the carbon source, stirring speed of cultures, temperature and time of incubation,nitro-gen  sources,  and  pH  of  medium.  In  the  present  study,  pigment  production  was  assessed  using  two  types  of  casein  as  substrate; pigment yield was greater when casein precipitated with vinegar was used as substrate than when high purity casein was. The maxi-mum prodigiosin production was achieved with 10 g/L casein at pH 8.0. Additionally, supplementation of culture media with glu-cose was found to considerably decrease prodigiosin production and growth inhibition of Staphylococcus aureus, which is directly related to pigment yield. Production in stirred-tank bioreactor at 0.75 vvm aeration was higher than that at 0.5 and 1.0 vvm. Sub-strate type, concentration and pH affected pigment production in Erlenmeyer flasks, whereas aeration rate influenced pigment pro-duction in a stirred-tank bioreactor.

scholarly journals Bridge Damage Detection Approach Using a Roving Camera Technique

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1246 ◽  
Author(s):  
Darragh Lydon ◽  
Myra Lydon ◽  
Rolands Kromanis ◽  
Chuan-Zhi Dong ◽  
Necati Catbas ◽  
...  
Keyword(s):  
Low Cost ◽  
Normal Operation ◽  
Laboratory Model ◽  
Traffic Patterns ◽  
Operational Conditions ◽  
Bridge Model ◽  
Detection Approach ◽  
Bridge Damage ◽  
Computer Vision Technology ◽  
Climate Events

Increasing extreme climate events, intensifying traffic patterns and long-term underinvestment have led to the escalated deterioration of bridges within our road and rail transport networks. Structural Health Monitoring (SHM) systems provide a means of objectively capturing and quantifying deterioration under operational conditions. Computer vision technology has gained considerable attention in the field of SHM due to its ability to obtain displacement data using non-contact methods at long distances. Additionally, it provides a low cost, rapid instrumentation solution with low interference to the normal operation of structures. However, even in the case of a medium span bridge, the need for many cameras to capture the global response can be cost-prohibitive. This research proposes a roving camera technique to capture a complete derivation of the response of a laboratory model bridge under live loading, in order to identify bridge damage. Displacement is identified as a suitable damage indicator, and two methods are used to assess the magnitude of the change in global displacement under changing boundary conditions in the laboratory bridge model. From this study, it is established that either approach could detect damage in the simulation model, providing an SHM solution that negates the requirement for complex sensor installations.

scholarly journals Enhanced Oil Removal by a Non-Toxic Biosurfactant Formulation

Energies ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 467
Author(s):  
Emília Mendes da Silva Santos ◽  
Isabela Regina Alvares da Silva Lira ◽  
Hugo Moraes Meira ◽  
Jaciana dos Santos Aguiar ◽  
Raquel Diniz Rufino ◽  
...  
Keyword(s):  
Room Temperature ◽  
Low Cost ◽  
Aeration Rate ◽  
Oil Removal ◽  
Central Composite Rotatable Design ◽  
Chemical Methods ◽  
Ph Values ◽  
Spectroscopic Analyses ◽  
New Formulation ◽  
Central Composite

In this study, a new formulation of low-cost, biodegradable, and non-toxic biosurfactant by Candida sphaerica UCP 0995 was investigated. The study was conducted in a bioreactor on an industrial waste-based medium, and a central composite rotatable design was used for optimization. The best results, namely a 25.22 mN/m reduction in surface tension, a biosurfactant yield of 10.0 g/L, and a critical micelle concentration of 0.2 g/L, were achieved in 132 h at an agitation speed of 175 rpm and an aeration rate of 1.5 vvm. Compositional and spectroscopic analyses of the purified biosurfactant by chemical methods, Fourier transform infrared spectroscopy, and nuclear magnetic resonance suggested that it is a glycolipid-type biosurfactant, and it showed no cytotoxicity in the MTT assay. The biosurfactant, submitted to different formulation methods as a commercial additive, remained stable for 120 days at room temperature. Tensioactive properties and stability were evaluated at different pH values, temperatures, and salt concentrations. The biosurfactant obtained with all formulation methods demonstrated good stability, with tolerance to wide ranges of pH, temperature and salinity, enabling application under extreme environmental conditions. Bioremediation tests were performed to check the efficacy of the isolated biosurfactant and the selected microbial species in removing oil from soil. The results demonstrated that the biosurfactant produced has promising properties as an agent for the bioremediation of contaminated soil.

scholarly journals Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Han Wang ◽  
Gloria M. Conover ◽  
Song-I Han ◽  
James C. Sacchettini ◽  
Arum Han
Keyword(s):  
Drug Treatment ◽  
Single Cell ◽  
Mycobacterium Smegmatis ◽  
Culture Media ◽  
Low Cost ◽  
Bacterial Pathogen ◽  
Growth Dynamics ◽  
Time Lapse ◽  
Cell Phenotype

AbstractAnalysis of growth and death kinetics at single-cell resolution is a key step in understanding the complexity of the nonreplicating growth phenotype of the bacterial pathogen Mycobacterium tuberculosis. Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. This technology was successfully applied to monitor the real-time growth dynamics of the fast-growing model strain Mycobacterium smegmatis (M. smegmatis) while subjected to drug treatment regimens during continuous culture for 48 h inside the microfluidic device. A clear morphological change leading to significant swelling at the poles of the bacterial membrane was observed during drug treatment. In addition, a small subpopulation of cells surviving treatment by frontline antibiotics was observed to recover and achieve robust replicative growth once regular culture media was provided, suggesting the possibility of identifying and isolating nonreplicative mycobacteria. This device is a simple, easy-to-use, and low-cost solution for studying the single-cell phenotype and growth dynamics of mycobacteria, especially during drug treatment.

scholarly journals “CLUPS”: A New Culture Medium for the Axenic Growth ofEntamoeba histolytica

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
F. Gonzalez-Salazar ◽  
I. Meester ◽  
F. J. Guzmán De La Garza ◽  
L. H. De La Garza-Salinas ◽  
A. Sampayo-Reyes ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
The Novel ◽  
Phospholipase Activity ◽  
Major Health Problem ◽  
Beef Liver ◽  
Major Health ◽  
Liver Abscesses ◽  
Therapeutic Tools

Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium forEntamoeba histolyticawhich allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS),E. histolyticatrophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters.E. histolyticatrophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

Effect of fatty acids on cellulase production by Penicillium funiculosum and its mutants

1987 ◽  
Vol 9 (5) ◽  
pp. 301-304 ◽  
Author(s):  
M. V. Deshpande ◽  
M. C. Srinivasan ◽  
S. S. Deshmukh
Keyword(s):  
Fatty Acids ◽  
Cellulase Production ◽  
Penicillium Funiculosum

Intensive 2-phenylethanol production in a hybrid system combined of a stirred tank reactor and an immersed extraction membrane module

2014 ◽  
Vol 68 (12) ◽  
Author(s):  
Mário Mihaľ ◽  
Ramiro Goncalves ◽  
Jozef Markoš
Keyword(s):  
Hybrid System ◽  
Hollow Fiber Membrane ◽  
Water Solution ◽  
Product Inhibition ◽  
Membrane Module ◽  
Membrane Extraction ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Operational Conditions ◽  
Biotransformation Process

AbstractBioconversion of l-phenylalanine to 2-phenylethanol using Saccharomyces cerevisiae is connected with the growth of biomass strongly limited by product inhibition. Therefore, fermentation can proceed only at low conversions of l-phenylalanine with very low yield of the desired product, which allows reaching the maximum concentration of 2-phenylethanol, 4 g L−1, in an ordinary batch, fed-batch, or chemostat bioreactor. To minimize capital and operating costs in the bioproduction of chemical specialties where the product inhibits the bioreaction, using a hybrid system based on the application of membrane extraction integrated in the bioreactor to remove the product is a suitable solution. Integration can be done by an external module for membrane extraction or, as a more efficient solution, by an extraction membrane module immersed directly in the bioreactor. Such a hybrid system can be used to remove 2-phenylethanol from the fermentation media and thus to overcome the product inhibition of the biotransformation process. In this paper, a hybrid system consisting of a stirred tank bioreactor (3.5 L) and an immersed extraction hollow fiber membrane module was studied. In the proposed system, the kinetics of 2-phenylethanol extraction from a water solution with and without biomass in the bioreactor to alkanes at different operational conditions was measured. Extraction kinetics was compared with the predictions obtained by a mathematical model. In the hybrid system, two extractive biotransformation experiments were performed and compared with that without product removal. Experimental data were also mathematically predicted with good accuracy between the simulation and the experiment.

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