scholarly journals Electrophoretic Analysis of Indian Isolates ofMycoplasma agalactiaeandMycoplasma bovisby SDS-PAGE and Immunoblotting

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Amit Kumar ◽  
N. C. Srivastava ◽  
V. P. Singh ◽  
Jai Sunder
Keyword(s):  
Biochemical Characterization ◽  
Protein Profile ◽  
Clinical Signs ◽  
Electrophoretic Analysis ◽  
Sheep And Goats ◽  
Contagious Agalactia ◽  
Sds Page ◽  
Diagnostic Values ◽  
Immunogenic Proteins ◽  
Species Specific

Mycoplasma agalactiaeandMycoplasma bovisboth are responsible for respiratory conditions in sheep and goats.M. agalactiaeis a major pathogen of sheep and goats and accounts for almost 90% of outbreaks of contagious agalactia syndrome in goats and almost 100% in sheep. On the basis of clinical signs and cultural, morphological, and biochemical characterization it is almost impossible to differentiate between both the species. Moreover, due to presence of genomic and proteomic similarity most of the time routine diagnostic tests fail to differentiate between them. Hence the present study was conducted to find out the protein profile of isolates of both the species by SDS-PAGE and to find out the cross-reacting as well as differentiating immunogenic proteins by Immunoblotting, which can be of immunoprophylactic as well as diagnostic values. The study revealed 6-7 major immunogenic cross-reactive proteins with the presence of two important non-cross-reacting species specific polypeptides particularly 25.50 and 24.54 kDa inM. agalactiaeandM. bovis, respectively, that might be of diagnostic values.

scholarly journals Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA

2004 ◽  
Vol 64 (2) ◽  
pp. 317-326 ◽  
Author(s):  
J. A. de O. Rodrigues ◽  
J. F. Höfling ◽  
F. C. A. Tavares ◽  
K. M. R. Duarte ◽  
R. B. Gonçalves ◽  
...  
Keyword(s):  
Candida Species ◽  
Profile Analysis ◽  
Protein Profile ◽  
Negative Control ◽  
Yeast Cells ◽  
Sds Page ◽  
Differential Medium ◽  
Oral Yeasts ◽  
Species Specific ◽  
High Level

The purpose of this work was to evaluate biochemical and serological methods to characterize and identify Candida species from the oral cavity. The strains used were five Candida species previously identified: C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. tropicalis, and Kluyveromyces marxianus, as a negative control. The analyses were conducted through the SDS-PAGE associated with statistical analysis using software, chromogenic medium, and CHROMagar Candida (CA), as a differential medium for the isolation and presumptive identification of clinically important yeasts and an enzyme-linked immunoabsorbent assay (ELISA), using antisera produced against antigens from two C. albicans strains. This method enabled the screening of the three Candida species: C. albicans, C. tropicalis, and C. Krusei, with 100% of specificity. The ELISA using purified immunoglobulin G showed a high level of cross-reaction against protein extracts of Candida species. The SDS-PAGE method allowed the clustering of species-specific isolates using the Simple Matching coefficient, S SM = 1.0. The protein profile analysis by SDS-PAGE increases what is known about the taxonomic relationships among oral yeasts. This methodology showed good reproducibility and allows collection of useful information for numerical analysis on information relevant to clinical application, and epidemiological and systematical studies.

scholarly journals Protein Profile Study of Some Nigerian Sesame (Sesamum indicum L.) Accessions

2015 ◽  
Vol 3 (2) ◽  
pp. 322-329 ◽  
Author(s):  
Gbenga Olorunshola Alege
Keyword(s):  
Genetic Diversity ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Sesamum Indicum ◽  
Genomic Changes ◽  
Sds Page ◽  
Sesame Genotypes ◽  
Species Specific ◽  
Total Seed

This study was carried out to investigate the genetic diversity among 23 sesame (Sesamum indicum L.) accessions obtained from different agro-ecological localities from 10 different states across 4 geopolitical zones in Nigeria using evidence from Sodium Dodecyl Polyacrylamide Gel Electrophoresis (SDS-PAGE). Total seed protein of the studied plants resolved on 12% SDS-PAGE showed variations in numbers and intensity of bands among the different sesame accessions. Thirteen (13) major bands were recorded in this study. Lack of unique band and presence of common band (band 7) among the 23 studied sesame accessions indicate some levels of genetic affinity and evidence of common evolutionary origin of the sesame genotypes. This band can therefore be tagged as species specific band for discriminating Sesamum indicum. Cluster analysis grouped the 23 sesame genotypes into two clusters with similarity coefficient ranging from 0.42 to 0.96 which indicates existence of genetic diversity; therefore there is ample opportunity for improving the 23 sesame genotypes. Variations in protein bands observed among the 23 studied plants could be attributed to genomic changes taken place during species diversification. It can be concluded that genetic diversity existed among Nigerian sesame for the improvement of characters of interest. Accessions 9 (YOL), 15(OTT), 22 (OFF) and 23 (JAL) are therefore recommended for used in future breeding programs for the development of improved sesame varieties.Int J Appl Sci Biotechnol, Vol 3(2): 322-329 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12734

scholarly journals Detection And Molecular Characterization of Theileria Ovis in Sheep And Goats with Clinical The Ileriosis in Kurdistan, Iraq

2020 ◽  
Vol 23 (2) ◽  
pp. 69-78
Author(s):  
Zuber Ismael Hassen ◽  
Azad Abdullah Meerkhan
Keyword(s):  
Disease Transmission ◽  
Clinical Signs ◽  
Kurdistan Region ◽  
Single Test ◽  
Molecular Method ◽  
Blood Samples ◽  
Sheep And Goats ◽  
Specific Pcr ◽  
Species Specific

This study was carried out to detect Theileria infection in sheep and goats in Kurdistan region, Iraq from June 2019 to April 2020. Molecular method was used to identify Theileria species. Sixty- seven blood samples were taken from 45 sheep and 22 goats based on clinical signs of theileriosis during tick activating season. The 67 samples were PCR edm and as a result, 20 species-specific PCR were positives (26.67% (12/20) were Theileria ovis in sheep and 36.36% (8/20) were from goats). The results of the gene analysis in the current study were registered in NCBI under four accession numbers (MN889986, MN889987, MN889988 and MN889989), which shows that sheep and goats can be infected with Theileria ovis. This is the first report of Theileria species in goats with clinical theileriosis in Kurdistan, so the gene flow and disease transmission between sheep and goats is most expected. PCR is a useful diagnostic tool to detect ovine theileriosis with a single test and suggested that T. ovis is the dominant piroplasmid agent in Erbil. In addition, it revealed that sheep is very susceptible to theileriosis than goats

scholarly journals Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds

2007 ◽  
Vol 76 (1) ◽  
pp. 71-77 ◽  
Author(s):  
D. Zendulková ◽  
A. Madanat ◽  
P. Lány ◽  
K. Rosenbergová ◽  
Z. Pospíšil
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Signs ◽  
Mycoplasma Species ◽  
Sample Collection ◽  
Chain Reaction ◽  
Mycoplasma Agalactiae ◽  
Sheep And Goats ◽  
Contagious Agalactia ◽  
Polymerase Chain ◽  
First Time

The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan.

scholarly journals Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis

2015 ◽  
Vol 87 (1) ◽  
pp. 455-462 ◽  
Author(s):  
JULIANA L. DREYFUSS ◽  
CAIO V. REGATIERI ◽  
BRUNO COELHO ◽  
JOSÉ B. BARBOSA ◽  
DENISE DE FREITAS ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Protein Profile ◽  
Clinical Signs ◽  
Tear Fluid ◽  
Specific Test ◽  
Tear Proteins ◽  
Viral Culture ◽  
Sds Page ◽  
The World ◽  
Acute Conjunctivitis

The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.

scholarly journals 442 PB 247 BIOCHEMICAL CHARACTERIZATION OF CULTIVATED OPUNTIA SPECIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 494e-494
Author(s):  
J.O. Kuti ◽  
C.M. Galloway
Keyword(s):  
Biochemical Characterization ◽  
Protein Profile ◽  
Seed Proteins ◽  
Spectrophotometric Analysis ◽  
Protein Profiles ◽  
Genetic Studies ◽  
Banding Patterns ◽  
Molecular Weights ◽  
Sds Page

The use of protein profiles and isozyme banding patterns as genetic markers in cultivated Opuntia species was investigated using SDS-PAGE and spectrophotometric analysis of seeds and stem (cladode) tissues. Twenty morphologically different entries belonging to six Opuntia species were analyzed for total protein profile and three enzyme systems (superoxide dismustase [SOD], phosphoglucomutase [PGM] and UDPG ppase). Seed proteins, mostly low molecular weights, were 3-fold that of cladode proteins. Marked differences in protein molecular weight were found among the entries. PGM activity, found only in the cladode tissues, differred among the entries. No UDPG ppase activity was found in either seeds or cladode tissues. Within the entries surveyed, identical SOD banding patterns were observed indicating some degree of similarity among the species. The preliminary results suggest that isozyme and protein profiles can be used as markers in genetic studies of cultivated Opuntia species.

scholarly journals Comparative Analysis of Electrophoretic Profile of Major Proteins of Milk from Alpine and Carpathian Goats

2017 ◽  
Vol 74 (1) ◽  
pp. 20 ◽  
Author(s):  
Alina NASALEAN ◽  
Laurentiu OGNEAN ◽  
Sergiu MUNTEAN ◽  
Stefana BALICI ◽  
Horea MATEI
Keyword(s):  
Protein Profile ◽  
Milk Proteins ◽  
Goat Milk ◽  
Protein Composition ◽  
Raw Milk ◽  
Biologically Active ◽  
Protein Fractions ◽  
Animal Nutrition ◽  
Sds Page ◽  
Percentage Data

The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and biologically active values. The proposal is to comparatively analyse the protein profile of milk. The milk was collected from two different breeds: French Alpine and Romanian Carpathian. During March and April 2016 there were collected samples of raw milk in hygienic and sanitation conditions. There were two lots: first lot has 10 Carpathian goats and the second lot has 10 Alpine goats. The protein composition of goat milk was established with SDS-PAGE, after the evaluation of the total proteins’ concentration with the Bradford method. The quantitative and percentage data obtained with electrophoresis revealed few differences between those 8 identified protein fractions. Between those two lots, regarding the levels of β-CN, k-CN and β-lactoglobulines there were significant differences. The other protein fractions have values almost identical. Statistical analysis of obtained data shaped the differences in the protein profile at those two breeds. Based on those differences it is to note the superior potential of the Alpine breed regarding the content in biologically active milk proteins. Regarding the obtained data, this study brings new contributions for the evaluation and analysis of protein profile as a nutritive and biologically active component of goat milk, confirming its character as a functional aliment.

scholarly journals Microchip Electrophoretic Analysis of Phaseolin Patterns and Its Comparison with Currently Used SDS-PAGE Techniques

2013 ◽  
Vol 76 (17-18) ◽  
pp. 1163-1169
Author(s):  
Emanuel Marques da Silva ◽  
Teresa Maria Marques dos Santos ◽  
José Filipe Teixeira Ganança ◽  
Jan Jacek Slaski ◽  
Miguel Â. A. Pinheiro de Carvalho
Keyword(s):  
Electrophoretic Analysis ◽  
Sds Page

Numerical electrophoretic analysis and the behavior of larval proteins among Aedes mosquitoes

1979 ◽  
Vol 57 (5) ◽  
pp. 979-982 ◽  
Author(s):  
Emmanuel C. Igbokwe
Keyword(s):  
Collective Behavior ◽  
Protein Complex ◽  
Related Species ◽  
Electrophoretic Analysis ◽  
Behavioral Profile ◽  
Aedes Mosquitoes ◽  
Molecular Behavior ◽  
Interspecific Divergence ◽  
Molecular Divergence ◽  
Species Specific

Species-specific patterns of larval protein electrophoregrams obtained among three species of Aedes mosquitoes were analyzed numerically. A behavioral profile was derived and illustrated for the larval protein complex of each species. Patterns of interspecific divergence in molecular behavior not detectable otherwise from the electrophoregrams were evident in the behavioral profiles of the proteins. The degree of electrophoretic correspondence obtained from the number of shared fractions among the species differs from that derived from the collective behavior of proteins. The numerical and graphic approach to the interpretation of protein electrophoregrams offers another parameter for gauging molecular divergence among related species of insects.

scholarly journals Mycoplasma agalactiae: The Sole Cause of Classical Contagious Agalactia?

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1782
Author(s):  
Sergio Migliore ◽  
Roberto Puleio ◽  
Robin A. J. Nicholas ◽  
Guido R. Loria
Keyword(s):  
Diagnostic Tests ◽  
Clinical Signs ◽  
Small Ruminants ◽  
Molecular Methods ◽  
The Other ◽  
Animal Disease ◽  
Mycoplasma Agalactiae ◽  
Contagious Agalactia ◽  
Mycoplasma Capricolum ◽  
Mycoplasma Mycoides

Contagious agalactia (CA) is suspected when small ruminants show all or several of the following clinical signs: mastitis, arthritis, keratoconjunctivitis and occasionally abortion. It is confirmed following mycoplasma isolation or detection. The historical and major cause is Mycoplasma agalactiae which was first isolated from sheep in 1923. Over the last thirty years, three other mycoplasmas (Mycoplasma mycoides subsp. capri, Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens) have been added to the etiology of CA because they can occasionally cause clinically similar outcomes though nearly always in goats. However, only M. agalactiae is subject to animal disease regulations nationally and internationally. Consequently, it makes little sense to list mycoplasmas other than M. agalactiae as causes of the OIE-listed CA when they are not officially reported by the veterinary authorities and unlikely to be so in the future. Indeed, encouraging countries just to report M. agalactiae may bring about a better understanding of the importance of CA. In conclusion, we recommend that CA should only be diagnosed and confirmed when M. agalactiae is detected either by isolation or molecular methods, and that the other three mycoplasmas be removed from the OIE Manual of Diagnostic Tests and Vaccines in Terrestrial Animals and associated sources.

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