Microchip Electrophoretic Analysis of Phaseolin Patterns and Its Comparison with Currently Used SDS-PAGE Techniques

Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the transformants due to the high auxin concentration codified by A. rhizogenes T-DNA incorporated to the plant genome.

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Jelly plug dissolution in Discoglossus pictus eggs (Anura) involves peroxidase-like activity and oxidative opening of disulphide bonds

Zygote ◽

10.1017/s0967199400001301 ◽

1993 ◽

Vol 1 (1) ◽

pp. 61-69 ◽

Cited By ~ 8

Author(s):

G. Pitari ◽

S. Dupré ◽

C. Fusco ◽

G. Maurizi ◽

C. Campanella

Keyword(s):

Hydrogen Peroxide ◽

Peroxidase Activity ◽

Electrophoretic Analysis ◽

Ringer Solution ◽

Cysteic Acid ◽

Jelly Coat ◽

Discoglossus Pictus ◽

Disulphide Bonds ◽

Sds Page

SummaryIn amphibian eggs the formation of a capsular chamber is one of the most striking events occurring either upon oviposition or after fertilisation. In the egg of the anuran Discoglossus pictus a capsular chamber forms following fertilisation or activation; the egg with its vitelline envelope rotates in this chamber according to gravity. Previous work showed that the chamber is the product of plug dissolution. The plug is a lens-shaped jelly coat, typical of Discoglossus, covering only part of the animal hemisphere. Its dissolution is caused by material released from the egg about 15 min after fertilisation through exocytosis of at least two types of vacuoles. Liquefaction of the plug correlates with the reduction of disulphide bonds present in the jelly matrix. In this study we investigated the nature of the substances released from the egg and some changes occurring in the plug during liquefaction. SDS-PAGE showed that the proteic profile of the plug changes dramatically after fertilisation, confirming proteic cleavage in the plug matrix during its dissolution. Through in vitro tests and electrophoretic analysis of the Ringer solution in which the egg exudate was collected, an increase in the activity of the solution was determined in the presence of hydrogen peroxide, and peroxidase activity was depicted in the egg exudate. The presence of free thiol groups and cysteic acid residues (or cysteine sulphinic acid) in the plugs of activated eggs was established, suggesting that during plug dissolution some disulphide bonds are oxidatively opened. This suggests that enzyme(s) with peroxidase activity are released following fertilisation. We surmise that such enzymes are contained in the intraovular vacuoles the exocytosis of which triggers the onset of plug liquefaction. The possible release of hydrogen peroxide from the egg is discussed.

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Simultaneous electrophoretic analysis of proteins of very high and low molecular weights using low-percentage acrylamide gel and a gradient SDS-PAGE gel

Electrophoresis ◽

10.1002/elps.200600141 ◽

2006 ◽

Vol 27 (20) ◽

pp. 3935-3938 ◽

Cited By ~ 13

Author(s):

Eduard Casas-Terradellas ◽

Francesc R. Garcia-Gonzalo ◽

Ouadah Hadjebi ◽

Ramon Bartrons ◽

Francesc Ventura ◽

...

Keyword(s):

Electrophoretic Analysis ◽

Molecular Weights ◽

Sds Page ◽

Very High

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Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development

Ciência Rural ◽

10.1590/s0103-84782011001200029 ◽

2011 ◽

Vol 41 (12) ◽

pp. 2224-2229 ◽

Cited By ~ 2

Author(s):

Camila Argenta Fante ◽

Patrícia de Fátima Pereira Goulart ◽

José Donizeti Alves ◽

Paôla de Castro Henrique ◽

Daniela Deitos Fries

Keyword(s):

Protein Content ◽

Chronic Diseases ◽

Total Protein ◽

Electrophoretic Analysis ◽

Stages Of Development ◽

Major Barrier ◽

Human Food ◽

Soil Flooding ◽

Sds Page ◽

Page Electrophoresis

The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.

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Buffalo mozzarella chemical composition and authenticity assessment by electrophoretic profiling

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4p1841 ◽

2017 ◽

Vol 38 (4) ◽

pp. 1841

Author(s):

Ben-Hur Ramos Ferreira Gonçalves ◽

Grazielly De Jesus Silva ◽

Daniele Gomes Conceição ◽

Antonio Silvio do Egito ◽

Sibelli Passini Barbosa Ferrão

Keyword(s):

Chemical Composition ◽

Electrophoretic Analysis ◽

Physicochemical Characteristics ◽

Buffalo Milk ◽

Water Soluble ◽

Cow Milk ◽

Cow’S Milk ◽

Cow's Milk ◽

Sds Page

Buffalo milk mozzarella is often adulterated by the addition of cow’s milk. The aim of this study was to assess the quality of buffalo milk mozzarella by using electrophoresis (SDS-PAGE) to detect the addition of cow’s milk. Reference cheeses were produced exclusively from combinations of buffalo and cow milk, and labeled reference treatment buffalo or cow (RTB and RTC, respectively). Standardized cheeses were made by combining buffalo milk and 2.5%, 5.0%, 10%, 20%, 30%, 40% or 50% cow’s milk. A total of 9 cheese formulations were produced and either frozen immediately (time 0) or after 20 days (time 20). Eighteen commercial samples of buffalo mozzarella were sampled between 0–20 days of production. The chemical composition (moisture, ash, fat in dry matter, protein, total solids and defatted dry extract) and physicochemical characteristics (acidity) of the cheeses were evaluated. Proteins and water-soluble peptides (WSP) extracted from RTB, RTC and from the commercial samples, and were analyzed by SDS-PAGE electrophoresis under denaturing conditions. It was not possible to detect the inclusion of cow’s milk by the cheeses’ chemical and physicochemical properties. However, the separation and detection of peptide and protein fractions of the cheese was possible by electrophoresis. The results of the electrophoretic analysis suggest that 28% of the commercial samples considered here had evidence of the addition of cow’s milk. The methodology described here is important to identify occurrences of fraud in buffalo mozzarella production.

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Genetic variability of three fish species of genus Puntius

Journal of Experimental and Applied Animal Sciences ◽

10.20454/jeaas.2017.1279 ◽

2017 ◽

Vol 2 (2) ◽

pp. 153

Author(s):

Abu-Almaaty A. H. ◽

Iman M. Bahgat ◽

Zaineb M. Al-Tahr

Keyword(s):

Molecular Weight ◽

Genetic Variation ◽

Genetic Variability ◽

Fish Species ◽

Genetic Similarity ◽

Molecular Detection ◽

Electrophoretic Analysis ◽

Sds Page ◽

New Varieties ◽

Issr Primers

The present study was aimed to evaluate the genetic variability among three fish species of genus Puntius, Puntius semifasciolatus, Puntius conchonius and Puntius oligolepis (family: Cyprinidae) using various genetic markers (SDS-Page and ISSR). The electrophoretic analysis of protein was revealed 29 bands of molecular weight ranging from 11 to 132KD with polymorphism 28%. A total of eleven ISSR primers produced 123 total bands their size ranged from 79 to 1185bp and the percentage of polymorphism ranged from 91% to 100%. Results analysis showed that the percentages of genetic relationship among three species were 33% and 47%. The results of the study revealed that Puntius semifasciolatus and Puntius conchonius have higher genetic similarity 47% while Puntius semifasciolatus and Puntius oligolepis exhibited lower genetic similarity 33%. Considering all the gained data, it is evident that molecular detection of SDS-Page and ISSR are suitable tools in assessing genetic variation among fish species. These markers provide interesting tools for breeding new varieties of Egyptian fish.

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Electrophoretic Analysis of Indian Isolates ofMycoplasma agalactiaeandMycoplasma bovisby SDS-PAGE and Immunoblotting

Veterinary Medicine International ◽

10.1155/2014/892421 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Amit Kumar ◽

N. C. Srivastava ◽

V. P. Singh ◽

Jai Sunder

Keyword(s):

Biochemical Characterization ◽

Protein Profile ◽

Clinical Signs ◽

Electrophoretic Analysis ◽

Sheep And Goats ◽

Contagious Agalactia ◽

Sds Page ◽

Diagnostic Values ◽

Immunogenic Proteins ◽

Species Specific

Mycoplasma agalactiaeandMycoplasma bovisboth are responsible for respiratory conditions in sheep and goats.M. agalactiaeis a major pathogen of sheep and goats and accounts for almost 90% of outbreaks of contagious agalactia syndrome in goats and almost 100% in sheep. On the basis of clinical signs and cultural, morphological, and biochemical characterization it is almost impossible to differentiate between both the species. Moreover, due to presence of genomic and proteomic similarity most of the time routine diagnostic tests fail to differentiate between them. Hence the present study was conducted to find out the protein profile of isolates of both the species by SDS-PAGE and to find out the cross-reacting as well as differentiating immunogenic proteins by Immunoblotting, which can be of immunoprophylactic as well as diagnostic values. The study revealed 6-7 major immunogenic cross-reactive proteins with the presence of two important non-cross-reacting species specific polypeptides particularly 25.50 and 24.54 kDa inM. agalactiaeandM. bovis, respectively, that might be of diagnostic values.

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Gastrin peptide isolation in broiler chicken

Indian Journal of Animal Research ◽

10.18805/ijar.6694 ◽

2015 ◽

Author(s):

A. K. Mandal ◽

R. K. Das ◽

A. Maity ◽

G. R. Sahoo

Keyword(s):

Molecular Weight ◽

Broiler Chicken ◽

Electrophoretic Analysis ◽

Distilled Water ◽

Protein Marker ◽

Molecular Weights ◽

Sds Page ◽

Reference Protein ◽

Relative Molecular Weight

The present study was undertaken to isolate gastrin peptide from the antral tissue of broiler chicken. The chicken antrums, i.e. tissue pieces from a narrow zone at gizzard – duodenal junction were collected, boiled in distilled water, followed by centrifugation at 0° C. The supernatant was collected, added to isopropanol and stirred overnight. After addition of dichloromethane, the aqueous phase was partitioned, aspirated and lyophilized. The electrophoretic analysis (SDS-PAGE) of the antral sample was carried out after running it along with a reference protein marker. Characterization of the antral extract revealed a total of eleven peptide bands having relative molecular weights (Mr) ranging from 4.6 to114.5 kDa, out of which peptides having Mr of 22.6 and 26.3 kDa were major ones. The protein or peptide band showing the lowest relative molecular weight (Mr, 4.6 kDa) was identified as the gastrin.

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Electrophoretic Analysis of Proteins from Mycoplasma hominis Strains Detected by SDS-PAGE, Two-dimensional Gel Electrophoresis and Immunoblotting

Microbiology ◽

10.1099/00221287-133-1-181 ◽

1987 ◽

Vol 133 (1) ◽

pp. 181-191 ◽

Cited By ~ 3

Author(s):

H. Andersen ◽

S. Birkelund ◽

G. Christiansen ◽

E. A. Freundt

Keyword(s):

Gel Electrophoresis ◽

Mycoplasma Hominis ◽

Electrophoretic Analysis ◽

Two Dimensional ◽

Two Dimensional Gel Electrophoresis ◽

Sds Page

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A New Bevacizumab Carrier for Intravitreal Administration: Focus on Stability

Pharmaceutics ◽

10.3390/pharmaceutics13040560 ◽

2021 ◽

Vol 13 (4) ◽

pp. 560

Author(s):

Daniela Chirio ◽

Elena Peira ◽

Simona Sapino ◽

Giulia Chindamo ◽

Simonetta Oliaro-Bosso ◽

...

Keyword(s):

Electrophoretic Analysis ◽

Vascular Endothelial ◽

Invasive Treatment ◽

Efficient Procedure ◽

Cell Compatibility ◽

Zeta Potentials ◽

Sustained Drug Delivery ◽

Sds Page ◽

Endothelial Growth Factor

Bevacizumab (BVZ) is a monoclonal antibody that binds to human vascular endothelial growth factor A (VEGF-A) and inhibits the interaction between VEGF-A and VEGF receptors, thus blocking the angiogenesis. Repeated intravitreal injections of BVZ for the treatment of ocular pathologies that present an excessive proliferation results in a low patience compliance. BVZ is specially indicated for the treatment of diabetic and degenerative retinopathy. In the present study, we designed lipid nanoparticles (NPs) as a BVZ sustained drug delivery system for reducing the frequency of administration. We used a simple and highly efficient procedure, “Cold dilution of microemulsions”, to obtain spherical NPs with mean diameters of 280–430 nm, Zeta potentials between −17 and −31 mV, and drug entrapment efficiencies between 50 to 90%. This study focused on the biochemical and biophysical stabilities of BVZ after entrapment in NPs. SDS-PAGE electrophoretic analysis and circular dichroism, dynamic light scattering, and scanning electron microscopy were used to characterize BVZ-loaded NPs. The biocompatibility was assessed by in vitro cell compatibility studies using the ARPE-19 cell line. Thus, in this work, a stable BVZ-loaded system was obtained. In addition, several studies have shown that BVZ is released slowly from the lipid matrix and that this system is biocompatible. The results are promising and the developed NPs could be exploited to create a new, potentially effective and minimally invasive treatment of intraocular diseases.

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