Effect of an Albumin-Coated Mesoporous Silicon Nanoparticle Platform for Paclitaxel Delivery in Human Lung Cancer Cell Line A549

Albumin-coated paclitaxel-mesoporous silicon nanoparticles (APMSN) were prepared to improve the anticancer effect in lung cancer by means of regulating the dissolution rate of paclitaxel (PTX). PTX was absorbed into the mesoporous structure of mesoporous silicon nanoparticles (MSN), which was defined as PMSN. PTX was proved to exist in an amorphous state in PMSN, which increased the dissolution rate of PTX. Albumin was coated on the surface of MSN to form AMSN; AMSN and PTX were mixed to form APMSN in order to achieve sustained release of PTX. Then, it was found that APMSN had more significant antiproliferate effects and induced more apoptotic proportion in comparison with PTX in A549 cells. Furthermore, the absorption mechanism of APMSN into A549 cells was investigated. Transmission electron microscopy (TEM) and laser scanning confocal microscopy (LSCM) showed that APMSN could cross the cell membrane and was taken into the cytoplasm quickly. Taken together, our results demonstrate that AMSN carriers have potential as nanodrug delivery systems in the treatment of lung cancer.

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The effect of antisense oligodeoxynucleotides targeting Aurora A kinase on cell proliferation and chemosensitivity to paclitaxel in human lung cancer cell line A549

Journal of Clinical Oncology ◽

10.1200/jco.2007.25.18_suppl.21147 ◽

2007 ◽

Vol 25 (18_suppl) ◽

pp. 21147-21147

Author(s):

R. Meng ◽

G. Wu ◽

K. Y. Yang ◽

J. Cheng

Keyword(s):

Lung Cancer ◽

Cell Line ◽

A549 Cells ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Human Lung Cancer ◽

Aurora A ◽

Lung Cancer Cell ◽

Mrna And Protein Expression ◽

Cell Inhibition

21147 Background: Aurora kinases representing a family of evolutionarily conserved mitotic serine/threonine kinases have been found elevated in lung andenocarcinoma cell line A549. It is suggested that the overexpression of Aurora A contributes to the carcinogenesis, chromosomal instability (CIN), and de-differentiation of lung cancers. To address its possibility as a therapeutic target for lung cancer, we employed the antisense oligodeoxynucleotide (ASODN) technique to inhibit Aurora A expression and investegate its effect on tumor growth and cell cycle of A549, as well as the chemosensitivity of paclitaxel. Methods: Aurora A ASODN was synthesized and transfected into A549 cells by lipofectAMINE 2000.Aurora A mRNA and protein expression were examined by reverse transcription -polymerase chain reaction (RT-PCR) and Western blot respectively.Cell cycle distribution was observed by flow cytometer.MTT assay was used to evaluate cell inhibition ratio before and after transfection. Results: The proliferation of the A549 cells was inhibited by Aurora A ASODN dose and time dependently. It was also observed that the IC50 of A549 cells after 48 hours’ treatment of ASODN was about 300nmol/L and under such circumstances, the Aurora A mRNA and protein expression significantly decreased (P<0.05), along with the induction of accumulation of cells in S phase and the G2-M transition. Furhermore, cell inhibition ratio of the combination of Aurora A ASODN and paclitaxel was higher significantly than paclitaxel(P<0.05) or Aurora A ASODN alone (P<0.05). Conclusions: Inhibition of Aurora A expression can results in the suppression of cell growth and chemosensitizing activity to paclitaxel in human lung cancer cell line A549. No significant financial relationships to disclose.

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Biodegradable Alginate-Chitosan Hollow Nanospheres for Codelivery of Doxorubicin and Paclitaxel for the Effect of Human Lung Cancer A549 Cells

BioMed Research International ◽

10.1155/2018/4607945 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 12

Author(s):

Liu Tao ◽

Jie Jiang ◽

Yu Gao ◽

Chao Wu ◽

Ying Liu

Keyword(s):

Lung Cancer ◽

Human Lung ◽

Hollow Structure ◽

A549 Cells ◽

Amorphous State ◽

Cell Uptake ◽

Human Lung Cancer ◽

Scanning Calorimetry ◽

Adsorption Method ◽

Multiple Drugs

A biodegradable alginate coated chitosan hollow nanosphere (ACHN) was prepared by a hard template method and used for codelivery of doxorubicin (DOX) and paclitaxel (PTX) to investigate the effect on human lung cancer A549 cells. PTX was loaded into the nanometer hollow structure of ACHN through adsorption method. DOX was coated on surface of ACHN through electrostatic interaction. Drug release studies exhibited a sustained-release effect. According to X-ray diffraction patterns (XRD), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FT-IR) analysis, DOX structure in the loading samples (DOX-PTX-ACHN) was of amorphous state while PTX was microcrystalline. Cytotoxicity experiments showed ACHN was nontoxic as carrier material and the combination of DOX and PTX in DOX-PTX-ACHN exhibited a good inhibiting effect on cell proliferation. Cell uptake experiments demonstrated that DOX-PTX-ACHN accumulated in the cytoplasm. Degradation experiments illustrated that ACHN was a biodegradable material. In summary, these results clearly indicate that ACHN can be utilized as a potential biomaterial to transport multiple drugs to be used in combination therapy.

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Resveratrol Encapsulation and Release from Pristine and Functionalized Mesoporous Silica Carriers

Pharmaceutics ◽

10.3390/pharmaceutics14010203 ◽

2022 ◽

Vol 14 (1) ◽

pp. 203

Author(s):

Simona Ioniţă ◽

Daniel Lincu ◽

Raul-Augustin Mitran ◽

Laila Ziko ◽

Nada K. Sedky ◽

...

Keyword(s):

Mesoporous Silica ◽

Dissolution Rate ◽

A549 Cells ◽

Amorphous State ◽

Chemical Properties ◽

Aqueous Solubility ◽

Human Lung Cancer ◽

Human Skin Fibroblast ◽

Functionalized Mesoporous Silica ◽

Ic50 Values

Resveratrol, a naturally occurring polyphenol, has attracted significant attention due to its antioxidant, cardioprotective and anticancer potential. However, its low aqueous solubility limits resveratrol bioavailability and use. In this work, different mesoporous silica matrices were used to encapsulate the polyphenol and to increase its dissolution rate. Pristine MCM-41, MCM-48, SBA-15, SBA-16, FDU-12 and MCF silica were obtained. The influence of SBA-15 functionalized with aminopropyl, isocyanate, phenyl, mercaptopropyl, and propionic acid moieties on resveratrol loading and release profiles was also assessed. The cytotoxic effects were evaluated for mesoporous carriers and resveratrol-loaded samples against human lung cancer (A549), breast cancer (MDA-MB-231) and human skin fibroblast (HSF) cell lines. The effect on apoptosis and cell cycle were assayed for selected resveratrol-loaded carriers. The polyphenol molecules are encapsulated only inside the mesopores, mostly in amorphous state. All materials containing either pristine or functionalized silica carriers increased polyphenol dissolution rate. The influence of the physico-chemical properties of the mesoporous carriers and resveratrol–loaded supports on the kinetic parameters was identified. Resv@SBA-15-SH and Resv@SBA-15-NCO samples exhibited the highest anticancer effect against A549 cells (IC50 values were 26.06 and 36.5 µg/mL, respectively) and against MDA-MB-231 (IC50 values were 35.56 and 19.30 µg/mL, respectively), which highlights their potential use against cancer.

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The effect of Thymbra spicata extract and its bioactive component Thymol on non-small-cells lung cancer cell line A549

Archives of Medical Science ◽

10.5114/aoms/136485 ◽

2021 ◽

Author(s):

Ardeshir Moayeri ◽

Shahram Mohammadpour ◽

Naser abbasi ◽

Ali Aidy ◽

Elahe Karimi ◽

...

Keyword(s):

Lung Cancer ◽

A549 Cells ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Small Cells ◽

Human Lung Cancer ◽

Ros Generation ◽

Bioactive Component ◽

High Concentrations ◽

Thymbra Spicata

IntroductionAlternative medicine is important in cancer treatment. The apoptotic effect of Thymol and extracted Thymol from Thymbra spicata on non-small-cells lung cancer was studied.Material and methodsThymol was evaluated in Thymbra spicata extract by HPCL. Cell viability was assessed by MTT method. DCF and flu3-AM probe was used for ROS and cai2+ analysis, respectively. Western blotting was performed to measure NOX2 and Bax/Bcl-2 ratio.ResultsObtained data showed that Thymol was 1.51 mg/g in Thymbra spicata extract. Treatment with Thymol and extracted Thymol from Thymbra spicata resulted in cell death at high concentrations [LC50= 111±4.5 and 119±5.2 μM, respectively]. Subsequently, Thymbra spicata extract and its bioactive component increased ROS and Cai2+ production, NOX2, and Bax/Bcl-2 ratio.ConclusionsThis study revealed the anticancer effects of Thymol and Thymbra spicata extract on non-small-cells lung cancer and at least part of that effect was related to the increase in the NOX2 and Bax/Bcl-2 ratio. Our results demonstrated that TSE and Thymol at high concentrations (180, 120, and 80 μM) decreased the growth of A549 cells. It appeared that cytotoxic activity was exerted through activation of NOX2, ROS generation, increase in Cai2+, and Bax/Bcl-2 ratio. Present results demonstrated that TSE and thymol may be potential therapeutic agents for human lung cancer.

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Antiproliferative and Apoptotic Effects ofSesbania grandifloraLeaves in Human Cancer Cells

BioMed Research International ◽

10.1155/2014/474953 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 36

Author(s):

Sankar Pajaniradje ◽

Kumaravel Mohankumar ◽

Ramya Pamidimukkala ◽

Srividya Subramanian ◽

Rukkumani Rajagopalan

Keyword(s):

Lung Cancer ◽

Cell Death ◽

Medicinal Plant ◽

Cancer Cell ◽

Human Cancer ◽

A549 Cells ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Human Lung Cancer ◽

Dapi Staining

Natural phytochemicals and their derivatives are good drug candidates for anticancer therapeutic approaches against multiple targets. We report here the initial findings from our studies on the anticancer properties of the leaves of the medicinal plantSesbania grandiflora. In the current study, five different solvent fractions from the leaves ofS. grandiflorawere tested on cancer cell lines such as MCF-7, HepG2, Hep-2, HCT-15, and A549. The methanolic fraction ofS. grandiflorawas found to exert potent antiproliferative effects especially in the human lung cancer cell line, A549. Caspase 3 was activated in the methanolic fraction treated A549 cells thereby leading to cell death by apoptosis. DAPI staining, DNA laddering, and decrease in mitochondrial membrane potential further confirmed the apoptotic mode of cell death. The high levels of ROS intermediates as evidenced by DCF-DA staining could have played a role in the apoptotic induction. Decrease in levels of cyclin D1 and decrease in the activation of NFkB were observed in A549 cells on treatment with methanolic fraction, giving a hint on the possible mechanism of action. These results prove that the medicinal plantS. grandifloracan be explored further for promising candidate molecules to combat cancer, especially lung cancer.

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The Effects of 2,2',4'-Trihydroxychalcone on the Cell Proliferation, Metastasis, and Apoptosis in Human Lung Cancer Cell Line A549

10.21203/rs.3.rs-859926/v1 ◽

2021 ◽

Author(s):

Jialin Sun ◽

Zhanqi Cao ◽

Shiwei Sun ◽

Zhonghua Sun ◽

Shuhong Sun ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Human Lung ◽

Vasculogenic Mimicry ◽

A549 Cells ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Human Lung Cancer ◽

Lung Cancer Cell ◽

Related Proteins

Abstract Objectives：Our study aimed to evaluate the antitumor effects of 2,2',4'-trihydroxychalcone (7a) on human lung cancer cell line A549. Methods：A549 cells were treated with different concentrations of 7a for different times. The cells without 7a were set as the negative control group. The cell proliferation, invasion, vasculogenic mimicry (VM) formation, heterogeneous adhesion, apoptosis were, respectively, measured by CCK-8, transwell invasion assay, vasculogenic mimicry assay, adhesion assay and flow cytometry. In addition, the expression of related proteins were examined via western blot or ELISA. Results：Our research found that 7a had a significant inhibitory effect on the survival rate of lung cancer A549 cells, while almost had no effect on human lung epithelial BEAS-2B cells and human venous endothelial cells (HUVECs). The migration rate, VM length, invasion and heterogeneous adhesion number of cells treated with 7a significantly decreased as the increase in concentration, while the apoptosis rate increased. Western blot analysis showed that 7a treatment significantly upregulated the expression of E-cadherin, cleaved PARP, Bax, caspase-3, and simultaneously downregulated the expression of MMP-2/9, Bcl-2, p-PI3K, p-Akt, p-MTOR, VEGF, E-selectin and N-cadherin. At the same time, ELISA results found that the pro-angiogenic factor VEGF level in culture media was reduced in the presence of 7a. Additionally, 7a could also reduce the nucleus NF-κB protein level, which would inhibit gene transcription of tumor activity-related proteins. Conclusion：7a might exert inhibitory effects on A549 cells via inhibiting cell proliferation, migration, VM formation, heterogeneous adhesion and inducing apoptosis through suppressing the PI3K/AKT/NF-κB signaling pathway, suggesting that 7a might have therapeutic potential for the treatment of lung cancer.

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