A Green Approach for the Synthesis of Silver Nanoparticles Using Ultrasonic Radiation’s Times in Sodium Alginate Media: Characterization and Antibacterial Evaluation

The synthesis of silver nanoparticles (Ag-NPs) was achieved by a simple green chemistry procedure using sodium alginate (Na-Alg) under ultrasonic radiation as a stabilizer and physical reducing agent. The effect of radiation time on the synthesis of Ag-NPs was carried out at room temperature until 720 min. The successful formation of Ag-NPs has been confirmed by UV-Vis, XRD, TEM, FESEM-EDX, zeta potential, and FT-IR analyses. The surface plasmon resonance band appeared at the range of 452–465 nm that is an evidence of formation of Ag-NPs. The XRD study showed that the particles are crystalline structure in nature, with a face-centered cubic (fcc) structure. The TEM study showed the Ag-NPs have average diameters of around 20.16–22.38 nm with spherical shape. The FESEM-EDX analysis confirmed the spherical shape of Ag-NPs on the surface of Alg and the element of Ag with the high purity. The zeta potential showed high stability of Alg/Ag-NPs especially after 720 min irradiation with value of −67.56 mV. The FT-IR spectrum confirmed that the Ag-NPs have been capped by the Alg with van der Waals interaction. The Alg/Ag-NPs showed the antibacterial activity against Gram-positive and Gram-negative bacteria. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field.

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Synthesis of silk sericin stabilized silver (Ag-Ser) nanoparticles using Tollens’ method and investigation of its colloidal stability

10.21203/rs.3.rs-1141192/v1 ◽

2021 ◽

Author(s):

Mert Saraçoğlu ◽

Begüm Bacınoğlu ◽

Sıddıka Mertdinç ◽

Servet Timur

Keyword(s):

Silver Nanoparticles ◽

Colloidal Stability ◽

Fourier Transforms ◽

Production Method ◽

Face Centered Cubic ◽

Ag Nps ◽

Transmission Electron ◽

Ft Ir ◽

Amine Complex ◽

Face Centered

Abstract In this study, sericin extracted from Bombyx mori silk cocoons was integrated into the well-known Tollens’ method for synthesizing Ag-NPs. Sericin successfully acted as a stabilizer while silver amine complex [Ag(NH3)2]+ was reduced by maltose. As a result, silver nanoparticles with high stability are formed. Possible functional groups related to the stabilization of NPs were investigated by Fourier-transforms infrared spectroscopy (FT-IR). Ag-Ser NPs were characterized by using particle size measurements based on dynamic light scattering (DLS) and transmission electron microscopy (TEM). According to the characterization investigations, Ag-Ser NPs have characteristic (111) face-centered cubic (FFC) plane and were spherical in shape with a narrow size distribution of 20.23 ±6.25 nm. Overall, the sericin-modified Tollens’ method for synthesizing Ag-NPs offers a simple and non-toxic production method to form nanoparticles. Colloidal stability of nanoparticles displays an essential role since their enhanced nano-properties can be diminished by an increase in size due to aggregation and agglomeration. Therefore, the effect of pH on particle stability was investigated through the surface charge of Ag-Ser NPs that was measured using a Zeta-potential analyzer. Results obtained from this study may extend the applicability of silver nanoparticles in biotechnological researches and a potential synthesis route for the application of Ag-Ser NPs as aseptic and therapeutic usages.

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Green Synthesis of Silver Nanoparticles Using Bilberry and Red Currant Waste Extracts

Processes ◽

10.3390/pr7040193 ◽

2019 ◽

Vol 7 (4) ◽

pp. 193 ◽

Cited By ~ 7

Author(s):

Antonio Zuorro ◽

Annalaura Iannone ◽

Stefano Natali ◽

Roberto Lavecchia

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Hydrodynamic Diameter ◽

Face Centered Cubic ◽

Ag Nps ◽

Stabilizing Agents ◽

Resonance Band ◽

Effects Of Temperature ◽

Face Centered ◽

Red Currant

The production of silver nanoparticles (Ag-NPs) from bilberry waste (BW) and red currant waste (RCW) extracts was studied. Red fruit extracts were obtained by treating BW and RCW with aqueous ethanol (50% v/v) at 40 °C. The formation of nanoparticles was monitored spectrophotometrically by measuring the intensity of the surface plasmon resonance band (SPR) of silver. The effects of temperature (20–60 °C) and pH (8–12) on the reaction kinetics and on the properties of Ag-NPs were investigated. Characterization by XRD and dynamic light scattering (DLS) techniques showed that Ag-NPs were highly crystalline, with a face-centered cubic structure and a hydrodynamic diameter of 25–65 nm. The zeta potential was in the range of −35.6 to −20.5 mV. Nanoparticles obtained from BW were slightly smaller and more stable than those from RCW. A kinetic analysis by the initial-rate method showed that there was an optimum pH, around 11, for the production of Ag-NPs. Overall, the results obtained suggest that BW and RCW can be advantageously used as a source of reducing and stabilizing agents for the green synthesis of Ag-NPs.

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Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity

Polymers ◽

10.3390/polym13203578 ◽

2021 ◽

Vol 13 (20) ◽

pp. 3578

Author(s):

Mohd Shahrul Nizam Salleh ◽

Roshafima Rasit Ali ◽

Kamyar Shameli ◽

Mohd Yusof Hamzah ◽

Rafiziana Md Kasmani ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Zeta Potential ◽

Gamma Radiation ◽

Nanoparticle Synthesis ◽

Reduction Process ◽

Spherical Shape ◽

Xrd Analysis ◽

Face Centered Cubic ◽

Ag Nps

The production of pure silver nanoparticles (Ag-NPs) with unique properties remains a challenge even today. In the present study, the synthesis of silver nanoparticles (Ag-NPs) from natural pullulan (PL) was carried out using a radiation-induced method. It is known that pullulan is regarded as a microbial polysaccharide, which renders it suitable to act as a reducing and stabilizing agent during the production of Ag-NPs. Pullulan-assisted synthesis under gamma irradiation was successfully developed to obtain Ag-NPs, which was characterized by UV-Vis, XRD, TEM, and Zeta potential analysis. Pullulan was used as a stabilizer and template for the growth of silver nanoparticles, while gamma radiation was modified to be selective to reduce silver ions. The formation of Ag-NPs was confirmed using UV–Vis spectra by showing a surface plasmon resonance (SPR) band in the region of 420–435 nm. As observed by TEM images, it can be said that by increasing the radiation dose, the particle size decreases, resulting in a mean diameter of Ag-NPs ranging from 40.97 to 3.98 nm. The XRD analysis confirmed that silver metal structures with a face-centered cubic (FCC) crystal were present, while TEM images showed a spherical shape with smooth edges. XRD also demonstrated that increasing the dose of gamma radiation increases the crystallinity at a high purity of Ag-NPs. As examined by zeta potential, the synthesized Ag-NP/PL was negatively charged with high stability. Ag-NP/PL was then analysed for antimicrobial activity against Staphylococcus aureus, and it was found that it had high antibacterial activity. It is found that the adoption of radiation doses results in a stable and green reduction process for silver nanoparticles.

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Green approach in gold, silver and selenium nanoparticles using coffee bean extract

Open Agriculture ◽

10.1515/opag-2020-0074 ◽

2020 ◽

Vol 5 (1) ◽

pp. 761-767

Author(s):

Reiyhaneh Abbasian ◽

Hoda Jafarizadeh-Malmiri

Keyword(s):

Spherical Shape ◽

Coffee Bean ◽

Hydrothermal Conditions ◽

Ag Nps ◽

Au Nps ◽

Stabilizing Agents ◽

E Coli ◽

Green Approach ◽

Gold Silver ◽

Ft Ir

AbstractGreen fabrication of metal nanoparticles (NPs), using natural reducing and stabilizing agents existed in plants and their derivatives, due to their unique properties, has gained more attention. The present study focuses on the synthesis of gold (Au), silver (Ag) and selenium (Se) NPs using coffee bean extract under hydrothermal conditions (1.5 atm and 121°C, for 15 min). Coffee bean extract obtained in 2 h processing using Clevenger apparatus and Fourier transform-infrared (FT-IR) spectroscopy indicated five highlighted peaks, namely, hydroxyl, amide, aromatic, alkane and ring groups. Dynamic light scattering analysis revealed that among three different NPs formed, fabricated Ag NPs had small particle size (153 nm) and high zeta potential value (16.8 mV). However, synthesized Au NPs had minimum polydispersity index (0.312). Results also indicated that fabricated Au, Se and Ag NPs had low antioxidant activity with values of 9.1, 8.9 and 8.7%, respectively. Morphological and antibacterial activity assessments, demonstrated that synthesized Ag, Au and Se NPs had spherical shape and high bactericidal activity against E. coli and S. aurous. Obtained results indicated that the synthesized NPs, can be utilized in various areas.

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Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

Journal of Nanomaterials ◽

10.1155/2020/3051308 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Margarita I. Skiba ◽

Victoria I. Vorobyova ◽

Alexander Pivovarov ◽

Natalya P. Makarshenko

Keyword(s):

Silver Nanoparticles ◽

Sodium Alginate ◽

Xrd Analysis ◽

Face Centered Cubic ◽

Colloidal Solutions ◽

Ag Nps ◽

Sem Images ◽

Spherical Powder ◽

Stabilizing Agent ◽

Vis Spectroscopy

The process of obtaining aqueous solutions of silver nanoparticles with the use of a low-temperature nonequilibrium contact plasma and stabilizing agent—polysaccharide (sodium alginate)—has been examined. The synthesized Ag NPs were characterized by using UV-Vis spectroscopy, dynamic light scattering (DLS), scanning electron microscope (SEM), and XRD analysis. The effect of concentration of Ag+, sodium alginate, duration of processing by plasma discharge, and pH of liquid on the production of silver nanoparticles has been studied. The results demonstrated that synthesis provides the formation of silver nanoparticles for investigated concentrations of Ag+ (0.3-3.0 mmol/l) and 5.0 g/l Na-Alg (pH=7–10) within 1–5 minutes. From the SEM images, the silver nanoparticles are found to be almost spherical. Powder XRD results reveal that Ag nanoparticles have a face-centered cubic crystal structure. Zeta potential of plasma-chemically obtained colloidal solutions at various concentrations of Ag+ ions and stabilizing agent varies from −32.8 to −39.3 mV, indicating the moderate stability of synthesized nanoparticles.

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Biosynthesis of Silver Nanoparticles Using Ganoderma Lucidum and Assessment of Antioxidant and Antibacterial Activity

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v5i4.18776 ◽

2017 ◽

Vol 5 (4) ◽

pp. 523-531 ◽

Cited By ~ 3

Author(s):

Milan Poudel ◽

Rabin Pokharel ◽

Sudip K.C. ◽

Suvash Chandra Awal ◽

Rajaram Pradhananga

Keyword(s):

Silver Nanoparticles ◽

Ganoderma Lucidum ◽

Fold Increase ◽

Diffusion Method ◽

Maximum Effect ◽

Face Centered Cubic ◽

Ag Nps ◽

Antimicrobial Studies ◽

Green Route ◽

Face Centered

There is an increasing interest these days in the green route of synthesis of metal nanoparticles using plant extract, fungus and bacterial- mediated eco-friendly materials. Silver nanoparticles were synthesized using an intracellular extract of Ganoderma lucidum, a mushroom from Nepal then characterization of silver nanoparticles was performed. The Ag-NPs thus formed show surface plasmonic resonance with a maximum absorption band at 420 nm. Their crystalline nature was confirmed as a face-centered cubic structure by the XRD, Furthermore, SEM revealed that they were in the size range of 10-30 nm and were spherical in shape. The possible biomolecule involved in the reduction and stabilization of Ag-NPs were believed to be oxidized polyphenol, and carbonyl group, amino acid residue. Ag-NPs exhibit good antioxidant activity but showed low antioxidant in comparison to the fungal extract alone, which was studied using DPPH antioxidant assay. The effect of the colloidal silver nanoparticles solution against six human pathological bacteria was carried out by Disc diffusion method. The zone of maximum inhibition was seen in Bacillus subtilis (17.0 ± 0.13 mm) and least effective against Escherichia coli (10.1 ± 0.2 mm). Further, the results showed that Ag-NPs in combination with antibiotics have better antibacterial effect as compared with Ag-NPs alone. The maximum effect with a 3.2 and 5.3 fold increase was seen in Gentamicin and Streptomycin respectively providing the synergistic role of Ag-NPs. The results of antimicrobial studies indicated that the Ag-NPs are the metal of choice and can be effectively used in combination with antibiotics in order to improve their efficiency against various pathogenic microbes. Int. J. Appl. Sci. Biotechnol. Vol 5(4): 523-531

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A green approach for the biosynthesis of powdered silver nanoparticles using leaf extract of Trigonella foenum-graecum L.: Characterization and antibacterial evaluation

Bangladesh Journal of Botany ◽

10.3329/bjb.v48i3.47722 ◽

2019 ◽

Vol 48 (3) ◽

pp. 427-436

Author(s):

T Rajkumar ◽

Jayanta Kumar Patra

Keyword(s):

Silver Nanoparticles ◽

Food Packaging ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Visible Spectrum ◽

Face Centered Cubic ◽

Green Approach ◽

Zones Of Inhibition ◽

Face Centered ◽

Trigonella Foenum Graecum L

Silver nanoparticles (AgNPs) were synthesized using aqueous leaf extract of Trigonella foenumgraecum as reducing and stabilizing agent. The ultraviolet-visible spectrum showed absorption peak at 480 nm. XRD pattern indicates the formation of face-centered cubic structure of silver nanoparticles. FESEM images indicate the presence of spherical silver nanoparticles with the particle size of ~90 nm. FTIR indicates that the participation of different functional groups present in the biomolecules is responsible for both reducing and stabilizing the formation of nanoparticles. The synthesized AgNPs demonstrated positive antibacterial activity against two different foodborne pathogenic bacteria (Escherichia coli O157:H7 ATCC 35150, Staphylococcus aureus ATCC 13565) with zones of inhibition of 9.20 ± 0.18 and 9.34 ± 0.11, respectively and MIC and MBC of 100 and >100 μg/ml, respectively. The synthesized AgNPs could serve as a candidate for development of antibacterial drugs or additive in the food packaging system for its application in medicine, cosmetics and food sector industries.

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Green synthesis of silver nanoparticles using tannins

Materials Science-Poland ◽

10.2478/s13536-014-0204-2 ◽

2014 ◽

Vol 32 (3) ◽

pp. 408-413 ◽

Cited By ~ 8

Author(s):

Pandian Raja ◽

Afidah Rahim ◽

Ahmad Qureshi ◽

Khalijah Awang

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Green Synthesis ◽

Silver Ions ◽

Face Centered Cubic ◽

X Ray Diffraction ◽

Spectroscopy Analysis ◽

Transmission Electron ◽

Ft Ir ◽

Face Centered

AbstractColloidal silver nanoparticles were prepared by rapid green synthesis using different tannin sources as reducing agent viz. chestnut (CN), mangrove (MG) and quebracho (QB). The aqueous silver ions when exposed to CN, MG and QB tannins were reduced which resulted in formation of silver nanoparticles. The resultant silver nanoparticles were characterized using UV-Visible, X-ray diffraction (XRD), scanning electron microscopy (SEM/EDX), and transmission electron microscopy (TEM) techniques. Furthermore, the possible mechanism of nanoparticles synthesis was also derived using FT-IR analysis. Spectroscopy analysis revealed that the synthesized nanoparticles were within 30 to 75 nm in size, while XRD results showed that nanoparticles formed were crystalline with face centered cubic geometry.

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Synthesis and Characterization of Florfenicol-silver Nanocomposite and its Antibacterial Activity against some Gram Positive and Gram-negative Bacteria

International Journal of Veterinary Science ◽

10.37422/ijvs/20.050 ◽

2020 ◽

Keyword(s):

Silver Nanoparticles ◽

Electron Microscope ◽

Zeta Potential ◽

Surface Area ◽

Spherical Shape ◽

Bet Surface Area ◽

Sonochemical Method ◽

Raman Spectrometry ◽

Silver Nanocomposite ◽

Morphology Characterization

In this paper, easy, rapid and cheap synthetic method was described for florfenicol-silver nanocomposite by sonochemical method. Florfenicol-silver nanocomposite was characterized based on three classes namely index, identification and morphology class. Index characterization was carried out by zeta sizing, BET surface area and zeta potential. Identification characterization was performed using X-ray diffraction (XRD) and Raman spectrometry. Morphology characterization was done utilizing transmission electron microscope (TEM), scanning electron microscope (SEM) and atomic force microscope (AFM). Characterization results showed zeta sizing of florfenicol was 30.44nm, while florfenicol-silver nanocomposite was 33.5 nm with zeta potential -14.1 and -18, respectively. BET surface area was found to be 13.3, 73.2 and 103.69 m2/g for florfenicol, silver nanoparticles and florfenicol-silver nanocomposite respectively. XRD and Raman charts confirmed the formation of florfenicol-silver nanocomposite without any contamination. TEM, SEM and AFM spectral data illustrated spherical to sub spherical shape of silver nanoparticles on cubic to sheet shape of florfenicol with size less than 50 nm. Antimicrobial activity was screened where the average zone of inhibitions caused by the prepared nanocomposite were 28.3 mm, 24 mm, 27.3 mm and 24 mm compared to 17.7 mm, 16 mm, 18.7 mm and 13.3 mm of the native drug and 13 mm, 10 mm, 14.3 mm and 15 mm of the used positive reference standards against E. coli, Salmonella typhymurium, Staphylococcus aureus and Staph.aureus MRSA respectively.

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Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals ◽

10.3390/cryst11020097 ◽

2021 ◽

Vol 11 (2) ◽

pp. 97

Author(s):

Suresh V. Chinni ◽

Subash C. B. Gopinath ◽

Periasamy Anbu ◽

Neeraj Kumar Fuloria ◽

Shivkanya Fuloria ◽

...

Keyword(s):

Electron Microscopy ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Zeta Potential ◽

Ethanolic Extract ◽

Spherical Shape ◽

Bacterial Strains ◽

X Ray ◽

Coccinia Indica ◽

Antibacterial Potential

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

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