scholarly journals Biosynthesis of Silver Nanoparticles Using Ganoderma Lucidum and Assessment of Antioxidant and Antibacterial Activity

2017 ◽  
Vol 5 (4) ◽  
pp. 523-531 ◽  
Author(s):  
Milan Poudel ◽  
Rabin Pokharel ◽  
Sudip K.C. ◽  
Suvash Chandra Awal ◽  
Rajaram Pradhananga
Keyword(s):  
Silver Nanoparticles ◽  
Ganoderma Lucidum ◽  
Fold Increase ◽  
Diffusion Method ◽  
Maximum Effect ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Antimicrobial Studies ◽  
Green Route ◽  
Face Centered

There is an increasing interest these days in the green route of synthesis of metal nanoparticles using plant extract, fungus and bacterial- mediated eco-friendly materials. Silver nanoparticles were synthesized using an intracellular extract of Ganoderma lucidum, a mushroom from Nepal then characterization of silver nanoparticles was performed. The Ag-NPs thus formed show surface plasmonic resonance with a maximum absorption band at 420 nm. Their crystalline nature was confirmed as a face-centered cubic structure by the XRD, Furthermore, SEM revealed that they were in the size range of 10-30 nm and were spherical in shape. The possible biomolecule involved in the reduction and stabilization of Ag-NPs were believed to be oxidized polyphenol, and carbonyl group, amino acid residue. Ag-NPs exhibit good antioxidant activity but showed low antioxidant in comparison to the fungal extract alone, which was studied using DPPH antioxidant assay. The effect of the colloidal silver nanoparticles solution against six human pathological bacteria was carried out by Disc diffusion method. The zone of maximum inhibition was seen in Bacillus subtilis (17.0 ± 0.13 mm) and least effective against Escherichia coli (10.1 ± 0.2 mm). Further, the results showed that Ag-NPs in combination with antibiotics have better antibacterial effect as compared with Ag-NPs alone. The maximum effect with a 3.2 and 5.3 fold increase was seen in Gentamicin and Streptomycin respectively providing the synergistic role of Ag-NPs. The results of antimicrobial studies indicated that the Ag-NPs are the metal of choice and can be effectively used in combination with antibiotics in order to improve their efficiency against various pathogenic microbes. Int. J. Appl. Sci. Biotechnol. Vol 5(4): 523-531

Green Synthesis of Silver Nanoparticles using the Leaves Extract of Filicium decipiens and its Anti- Microbial Activity

2021 ◽  
Vol 10 (3) ◽  
pp. 16-24
Author(s):  
Sherin Monichan ◽  
P. Mosae Selvakumar ◽  
Christine Thevamithra ◽  
M. S. A. Muthukumar Nadar ◽  
Jesse Joel
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Average Diameter ◽  
Diffusion Method ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Almost All

Silver nanoparticles has been used since ages, even till now it is exploited in almost all areas like medicine, textiles, industries, cosmetics, purification, dying and many more. There are many approaches which are used to synthesize silver nanoparticles. However, these approaches are either harmful to the environment or very costly. Therefore, green synthesis of silver nanoparticles (AgNPs) using leaves of Filicium decipiens eco-friendly and a very reliable method to procure AgNPs. Characterization of synthesized AgNPs were then done using UV-Vis spectroscopy and fluorescence which confirmed the formation of AgNPs, scanning electron microscope (SEM)confirmed its shape to be round and X-ray diffraction (XRD) determined its crystalline nature as face centered cubic structure. Furthermore, Dynamic Light Scattering (DLS) was also done in order to know the average diameter and zeta potential of AgNPs. However, it did not show potential results due to the aggregates formed during the green synthesis of AgNPs. In addition to this, anti-microbial test against bacteria such as gram negative (Escherichia. Coli) and gram positive (Bacillus.spc) were done using well-diffusion method and also its application of antimicrobial activity was tested over fabric to understand its application in textile industries. In both the cases, AgNPs showed more efficiency in gram negative bacteria than gram- positive.

scholarly journals Synthesis of silk sericin stabilized silver (Ag-Ser) nanoparticles using Tollens’ method and investigation of its colloidal stability

2021 ◽  
Author(s):  
Mert Saraçoğlu ◽  
Begüm Bacınoğlu ◽  
Sıddıka Mertdinç ◽  
Servet Timur
Keyword(s):  
Silver Nanoparticles ◽  
Colloidal Stability ◽  
Fourier Transforms ◽  
Production Method ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Transmission Electron ◽  
Ft Ir ◽  
Amine Complex ◽  
Face Centered

Abstract In this study, sericin extracted from Bombyx mori silk cocoons was integrated into the well-known Tollens’ method for synthesizing Ag-NPs. Sericin successfully acted as a stabilizer while silver amine complex [Ag(NH3)2]+ was reduced by maltose. As a result, silver nanoparticles with high stability are formed. Possible functional groups related to the stabilization of NPs were investigated by Fourier-transforms infrared spectroscopy (FT-IR). Ag-Ser NPs were characterized by using particle size measurements based on dynamic light scattering (DLS) and transmission electron microscopy (TEM). According to the characterization investigations, Ag-Ser NPs have characteristic (111) face-centered cubic (FFC) plane and were spherical in shape with a narrow size distribution of 20.23 ±6.25 nm. Overall, the sericin-modified Tollens’ method for synthesizing Ag-NPs offers a simple and non-toxic production method to form nanoparticles. Colloidal stability of nanoparticles displays an essential role since their enhanced nano-properties can be diminished by an increase in size due to aggregation and agglomeration. Therefore, the effect of pH on particle stability was investigated through the surface charge of Ag-Ser NPs that was measured using a Zeta-potential analyzer. Results obtained from this study may extend the applicability of silver nanoparticles in biotechnological researches and a potential synthesis route for the application of Ag-Ser NPs as aseptic and therapeutic usages.

scholarly journals Green Synthesis of Silver Nanoparticles Using Bilberry and Red Currant Waste Extracts

Processes ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 193 ◽  
Author(s):  
Antonio Zuorro ◽  
Annalaura Iannone ◽  
Stefano Natali ◽  
Roberto Lavecchia
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Hydrodynamic Diameter ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Stabilizing Agents ◽  
Resonance Band ◽  
Effects Of Temperature ◽  
Face Centered ◽  
Red Currant

The production of silver nanoparticles (Ag-NPs) from bilberry waste (BW) and red currant waste (RCW) extracts was studied. Red fruit extracts were obtained by treating BW and RCW with aqueous ethanol (50% v/v) at 40 °C. The formation of nanoparticles was monitored spectrophotometrically by measuring the intensity of the surface plasmon resonance band (SPR) of silver. The effects of temperature (20–60 °C) and pH (8–12) on the reaction kinetics and on the properties of Ag-NPs were investigated. Characterization by XRD and dynamic light scattering (DLS) techniques showed that Ag-NPs were highly crystalline, with a face-centered cubic structure and a hydrodynamic diameter of 25–65 nm. The zeta potential was in the range of −35.6 to −20.5 mV. Nanoparticles obtained from BW were slightly smaller and more stable than those from RCW. A kinetic analysis by the initial-rate method showed that there was an optimum pH, around 11, for the production of Ag-NPs. Overall, the results obtained suggest that BW and RCW can be advantageously used as a source of reducing and stabilizing agents for the green synthesis of Ag-NPs.

Green Synthesis of Starch-Stabilized Silver Nanoparticles and their Antibacterial Properties

2011 ◽  
Vol 236-238 ◽  
pp. 1945-1948 ◽  
Author(s):  
Xiang Hua Gao ◽  
Li Qiao Wei ◽  
Jing Wang ◽  
Bing She Xu
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Spherical Particles ◽  
Ring Test ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Green Route ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
20 Nm

Stable silver nanoparticles were synthesized from AgNO3through a simple green route using biodegradable and renewable starch as stabilizing agent. Nanoparticles have been studied for their formation, structure, morphology and size using UV–vis spectroscopy, X-ray diffraction (XRD) and HRTEM analysis. XRD showed that the nanoparticles were of face centered cubic structure. UV–Vis and HRTEM revealed that the spherical particles having radius 5-20 nm are mostly stabilized by starch. Further, antibacterial properties of these starch-stabilized nanoparticles show promising results for S.aureus in growth inhibition ring test.

scholarly journals A Green Approach for the Synthesis of Silver Nanoparticles Using Ultrasonic Radiation’s Times in Sodium Alginate Media: Characterization and Antibacterial Evaluation

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Miftah Faried ◽  
Kamyar Shameli ◽  
Mikio Miyake ◽  
Abdollah Hajalilou ◽  
Ali Zamanian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Sodium Alginate ◽  
Spherical Shape ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Green Approach ◽  
Resonance Band ◽  
Ft Ir ◽  
Face Centered

The synthesis of silver nanoparticles (Ag-NPs) was achieved by a simple green chemistry procedure using sodium alginate (Na-Alg) under ultrasonic radiation as a stabilizer and physical reducing agent. The effect of radiation time on the synthesis of Ag-NPs was carried out at room temperature until 720 min. The successful formation of Ag-NPs has been confirmed by UV-Vis, XRD, TEM, FESEM-EDX, zeta potential, and FT-IR analyses. The surface plasmon resonance band appeared at the range of 452–465 nm that is an evidence of formation of Ag-NPs. The XRD study showed that the particles are crystalline structure in nature, with a face-centered cubic (fcc) structure. The TEM study showed the Ag-NPs have average diameters of around 20.16–22.38 nm with spherical shape. The FESEM-EDX analysis confirmed the spherical shape of Ag-NPs on the surface of Alg and the element of Ag with the high purity. The zeta potential showed high stability of Alg/Ag-NPs especially after 720 min irradiation with value of −67.56 mV. The FT-IR spectrum confirmed that the Ag-NPs have been capped by the Alg with van der Waals interaction. The Alg/Ag-NPs showed the antibacterial activity against Gram-positive and Gram-negative bacteria. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field.

scholarly journals Green Synthesis and Characterization of Pullulan Mediated Silver Nanoparticles through Ultraviolet Irradiation

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2382 ◽  
Author(s):  
Muhammad Jamshed Khan ◽  
Suriya Kumari ◽  
Kamyar Shameli ◽  
Jinap Selamat ◽  
Awis Qurni Sazili
Keyword(s):  
Silver Nanoparticles ◽  
Uv Irradiation ◽  
Irradiation Time ◽  
Edible Films ◽  
Crystallographic Structure ◽  
Face Centered Cubic ◽  
Selected Area Electron Diffraction ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Lattice

Nanoparticles (NPs) are, frequently, being utilized in multi-dimensional enterprises. Silver nanoparticles (AgNPs) have attracted researchers in the last decade due to their exceptional efficacy at very low volume and stability at higher temperatures. Due to certain limitations of the chemical method of synthesis, AgNPs can be obtained by physical methods including sun rays, microwaves and ultraviolet (UV) radiation. In the current study, the synthesis of pullulan mediated silver nanoparticles (P-AgNPs) was achieved through ultraviolet (UV) irradiation, with a wavelength of 365 nm, for 96 h. P-AgNPs were formed after 24 h of UV-irradiation time and expressed spectra maxima as 415 nm, after 96 h, in UV-vis spectroscopy. The crystallographic structure was “face centered cubic (fcc)” as confirmed by powder X-ray diffraction (PXRD). Furthermore, high resolution transmission electron microscopy (HRTEM) proved that P-AgNPs were covered with a thin layer of pullulan, with a mean crystalline size of 6.02 ± 2.37. The average lattice fringe spacing of nanoparticles was confirmed as 0.235 nm with quasi-spherical characteristics, by selected area electron diffraction (SAED) analysis. These green synthesized P-AgNPs can be utilized efficiently, as an active food and meat preservative, when incorporated into the edible films.

scholarly journals Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1777 ◽  
Author(s):  
Md. Mahiuddin ◽  
Prianka Saha ◽  
Bungo Ochiai
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ftir Spectroscopy ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

scholarly journals Evaluation of Changes Induced in the Probiotic Escherichia coli M17 Following Recurrent Exposure to Antimicrobials

2021 ◽  
pp. 158-167
Author(s):  
M. J. A. Mbarga ◽  
I. V. Podoprigora ◽  
E. G. Volina ◽  
A. V. Ermolaev ◽  
L. A. Smolyakova
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Fold Increase ◽  
Bacterial Attachment ◽  
Diffusion Method ◽  
Cross Resistance ◽  
Microdilution Method ◽  
Similar Work

Introduction: It is already well known that the exposure of certain bacteria, pathogenic or not, to antimicrobials is likely to increase their virulence and induce the development of direct or cross resistance to antimicrobials, but there is almost no information available regarding probiotics. Aim: To assess the changes induced in susceptibility to antibiotics, biofilm formation, growth rate and relative pathogenicity in the probiotic Escherichia coli M17 (EC-M17) after long exposure to antimicrobials namely ampicillin, kanamycin, cefazolin and silver nanoparticles (AgNPs). Methods: After determining the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the 4 antimicrobials above-mentioned by the microdilution method, EC-M17 was exposed to increasing subinhibitory doses ranging from MIC/8 to MIC for 8 days. The susceptibility to antibiotics of the mutants obtained was assessed by the Kirby Bauer disc diffusion method, biofilm formation by the Congo red agar method and with crystal violet bacterial attachment assay, and relative pathogenicity was assessed using a Galleria melonella waxworm model. Results: Exposure to antimicrobials induces noticeable changes in EC-M17. The highest adaptation to antimicrobials was observed on AgNPs with 8-fold increase in MIC and 16-fold increase in MBC of AgNPs. EC-M17 exposed to ampicillin, kanamycin and silver nanoparticles became resistant to ampicillin, ceftazidime, ceftazidime/clavulanate and tetracycline while exposure to cefazolin induced a significant decrease in sensitivity to tetracycline and ampicillin and resistance to ceftazidime/clavulanate and ceftazidime. The strain exposed to ampicillin was the only one to produce more biofilm than the control strain and except the EC-M17 exposed to cefazolin, all other EC-M17 strains were more pathogenic on G. melonella model than the control. Conclusion: Data in this investigation suggest that repeated exposure of the probiotic EC-M17 to antimicrobials may induce changes in antimicrobials susceptibility, biofilm formation, growth rate, and relative pathogenicity. Therefore, as far as possible, the probiotic E. coli M17 should not be used in combination with antibiotics and further investigations are required to expand similar work on more probiotics in order to avoid resistance build-up which might be transmitted by horizontal transfer.

scholarly journals Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

2019 ◽  
Vol 31 (12) ◽  
pp. 2804-2810
Author(s):  
Anti Kolonial Prodjosantoso ◽  
Oktanio Sigit Prawoko ◽  
Maximus Pranjoto Utomo ◽  
Lis Permana Sari
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Silver Ions ◽  
Reduction Reaction ◽  
Face Centered Cubic ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Face Centered

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

scholarly journals Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sudip Some ◽  
Onur Bulut ◽  
Kinkar Biswas ◽  
Anoop Kumar ◽  
Anupam Roy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Silver Ions ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Beneficial Effects ◽  
Selected Area Electron Diffraction ◽  
Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

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