scholarly journals Antifungal Activity of Aqueous Extracts of Some Dominant Algerian Medicinal Plants

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Nasrine Salhi ◽  
Sultan Ayesh Mohammed Saghir ◽  
Valeria Terzi ◽  
Iman Brahmi ◽  
Naima Ghedairi ◽  
...  
Keyword(s):  
Wheat Yield ◽  
Fungal Species ◽  
Aqueous Extracts ◽  
Indigenous Plants ◽  
Mycelium Growth ◽  
Vitro Assay ◽  
Antifungal Properties ◽  
Aerial Parts ◽  
Cotula Cinerea

Aim. This study investigated the antifungal properties of aqueous extracts obtained from indigenous plants that grow spontaneously in the Northern Sahara of Algeria. The activities of these plants in controlling two fungal species that belong to Fusarium genus were evaluated in an in vitro assay. Materials and Methods. Fresh aerial parts of four plant species (Artemisia herba alba, Cotula cinerea, Asphodelus tenuifolius, and Euphorbia guyoniana) were collected for the preparation of aqueous extracts. Two levels of dilution (10% and 20%) of the pure extracts were evaluated against Fusarium graminearum and Fusarium sporotrichioides. Results. The results of this study revealed that the A. herba alba, C. cinerea, A. tenuifolius, and E. guyoniana aqueous extracts are effective at both concentrations of 10% and 20% for the Fusarium mycelia growth inhibition. In particular, A. tenuifolius extract is effective against F. graminearum, whereas F. sporotrichioides mycelium growth is strongly affected by the E. guyoniana 20% extract. The phytochemical characterization of the compositions of the aqueous extracts has revealed that the presence of some chemical compounds (tannins, flavonoids, saponins, steroids, and alkaloids) is likely to be responsible for the antifungal activities sought. Conclusion. The antifungal properties of A. herba alba, C. cinerea, A. tenuifolius, and E. guyoniana make these plants of potential interest for the control of fungi affecting both wheat yield and safety.

scholarly journals Phytochemical Screening of Alangium salviifolium (L.f.) Wangerin Showing Antifungal Properties Against Alternaria Spp.

2019 ◽  
Vol 2 (1) ◽  
pp. 1-4
Author(s):  
Mohd Aphajal ◽  
Mirza Jaish Beg
Keyword(s):  
Chemical Compounds ◽  
Fungal Species ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Alternaria Brassicae ◽  
Higher Plant ◽  
Vitro Assay ◽  
Antifungal Properties ◽  
Ethanol Extracts

The antifungal properties shown by different higher plant extracts are due to the presence of phytochemicals. The activities of Alangium solviifolium (L.f.) Wangerin root in controlling two fungal species that belong to Alternaria spp- Alternaria brassicae and A. brassicicola, the causal organisms of leaf blight of crucifers was evaluated in vitro assay. The aqueous extracts of different parts of the tested plant such as bark, stem, root, leaf and flower were screened against test fungi. Among them, the results of the study revealed that the aqueous extracts of A. solviifolium(L.f.) Wangerin root showed the inhibition of mycelial growth against test fungi completely (100%). The phytochemical analysis of active plant has revealed that the presence of some chemical compounds as alkaloids, terpenoids, phenol/tannins, saponins, flavonoids and glycosides in both aqueous and ethanol extracts is likely to be responsible for the antifungal activity against test fungi. This study confirms the presence of various bioactive compounds of an active plant.

scholarly journals Mutagenicity evaluation of Anastatica hierochuntica L. aqueous extract in vitro and in vivo

2017 ◽  
Vol 243 (4) ◽  
pp. 375-385 ◽  
Author(s):  
Siti Rosmani Md Zin ◽  
Zahurin Mohamed ◽  
Mohammed A Alshawsh ◽  
Won F Wong ◽  
Normadiah M Kassim
Keyword(s):  
Aqueous Extract ◽  
Positive Control ◽  
In Vitro Assay ◽  
In Vivo Study ◽  
Sufficient Evidence ◽  
Aqueous Extracts ◽  
Mutagenic Potential ◽  
Vitro Assay

Anastatica hierochuntica L. ( A. hierochuntica), a folk medicinal plant, was evaluated for mutagenic potential via in vitro and in vivo assays. The in vitro assay was conducted according to modified Ames test, while the in vivo study was performed according to Organisation for Economic Co-operation and Development guideline for mammalian erythrocyte micronucleus assay. Four groups ( n= 5 males and 5 females per group) Sprague Dawley rats were randomly chosen as the negative control, positive control (received a single intramuscular injection of cyclophosphamide 50 mg/kg), 1000 and, 2000 mg/kg A. hierochuntica aqueous extracts. All groups except the positive control were treated orally for three days. Findings of the in vitro assay showed mutagenic potential of AHAE at 0.04 and 0.2 mg/ml. However, no mutagenic effect was demonstrated in the in vivo study up to 2000 mg/kg. No significant reduction in the polychromatic and normochromatic erythrocytes ratio was noted in any of the groups. Meanwhile, high micronucleated polychromatic erythrocytes frequency was seen in cyclophosphamide-treated group only. These findings could perhaps be due to insufficient dosage of A. hierochuntica aqueous extracts to cause genetic damage on the bone marrow target cells. Further acute and chronic in vivo toxicity studies may be required to draw pertinent conclusion on the safety aspect of A. hierochuntica aqueous extracts consumption. Impact statement In this paper, we report on the mutagenicity evaluation of Anastatica hierochuntica aqueous extract. This is a significant research in view of the popularity of this herb consumption by the people across the globe despite of limited scientific evidence on its toxicity potential. This study is intended to encourage more extensive related research in order to provide sufficient evidence and guidance for determining its safe dosage.

scholarly journals Transcriptomic Insight into Terpenoid Biosynthesis and Functional Characterization of Three Diterpene Synthases in Scutellaria barbata

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2952 ◽  
Author(s):  
Huabei Zhang ◽  
Baolong Jin ◽  
Junling Bu ◽  
Juan Guo ◽  
Tong Chen ◽  
...  
Keyword(s):  
Average Length ◽  
Functional Characterization ◽  
Terpene Synthase ◽  
Terpenoid Biosynthesis ◽  
Vitro Assay ◽  
Scutellaria Barbata ◽  
Aerial Parts ◽  
Diterpene Synthase ◽  
Clerodane Diterpenoids

Scutellaria barbata (Lamiaceae) is an important medicinal herb widely used in China, Korea, India, and other Asian countries. Neo-clerodane diterpenoids are the largest known group of Scutellaria diterpenoids and show promising cytotoxic activity against several cancer cell lines. Here, Illumina-based deep transcriptome analysis of flowers, the aerial parts (leaf and stem), and roots of S. barbata was used to explore terpenoid-related genes. In total, 121,958,564 clean RNA-sequence reads were assembled into 88,980 transcripts, with an average length of 1370 nt and N50 length of 2144 nt, indicating high assembly quality. We identified nearly all known terpenoid-related genes (33 genes) involved in biosynthesis of the terpenoid backbone and 14 terpene synthase genes which generate skeletons for different terpenoids. Three full length diterpene synthase genes were functionally identified using an in vitro assay. SbTPS8 and SbTPS9 were identified as normal-CPP and ent-CPP synthase, respectively. SbTPS12 reacts with SbTPS8 to produce miltiradiene. Furthermore, SbTPS12 was proven to be a less promiscuous class I diterpene synthase. These results give a comprehensive understanding of the terpenoid biosynthesis in S. barbata and provide useful information for enhancing the production of bioactive neo-clerodane diterpenoids through genetic engineering.

Wound healing properties of aqueous extracts of Sargassum illicifolium: An in vitro assay

2019 ◽  
Vol 24 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Amal D. Premarathna ◽  
T.H. Ranahewa ◽  
S.K. Wijesekera ◽  
R.R.M.K.K. Wijesundara ◽  
Anura P. Jayasooriya ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Assay ◽  
Aqueous Extracts ◽  
Vitro Assay

scholarly journals Composition and Antioxidant Properties of the Essential Oil of the Endemic Cape Verdean Satureja forbesii

2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Risoleta Ortet ◽  
Erik L. Regalado ◽  
Olivier P. Thomas ◽  
Jorge A. Pino ◽  
Miguel D. Fernández ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vitro Assay ◽  
Cape Verde ◽  
Cape Verdean ◽  
Vitro Assay ◽  
Aerial Parts

The chemical composition of essential oil from the air-dried aerial parts of Satureja forbesii (Benth.) Briq. from Cape Verde was studied by GC and GC/MS. Thirty-nine volatile compounds were identified of which geranial (42.0%) and neral (31.2%) were the major constituents. Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant activities were evidenced.

scholarly journals Chemical Constituents of Equisetum Debile and their Cytotoxic Activity

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301
Author(s):  
Tran Huy Thai ◽  
Nguyen Quang Hung ◽  
Chau Van Minh ◽  
Nguyen Xuan Cuong ◽  
Pham Hai Yen ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cancer ◽  
Chemical Constituents ◽  
In Vitro Assay ◽  
The Other ◽  
Human Cancer Cell ◽  
Vitro Assay ◽  
Human Cancer Cell Lines ◽  
Aerial Parts

A new phenyl glycoside, equisetumoside D (1), was isolated from the aerial parts of Equisetum debile, along with equisetumoside B (2), dehydrovomifoliol (3), corchoionoside C (4), (-)-isolariciresinol-3a-O-β-D-glucopyranoside (5), and kaempferol 3-O-sophoroside-7-O-β-D-glucopyranoside (6). Their structures were elucidated by NMR spectroscopic and MS experiments. Compound 1 was found to be cytotoxic against both tested human cancer cell lines, hepatocellular carcinoma (Hep-G2, IC50: 1.12 μg/mL) and rhabdosarcoma (RD, IC50: 0.25 μg/mL), while the other compounds showed no activity against these cell lines by in vitro assay.

scholarly journals Antifungal activities of quince seed mucilage hydrogel decorated with essential oils of Nigella sativa, Citrus sinensis and Cinnamon verum

2021 ◽  
Author(s):  
Mehdi Mirzaii ◽  
Marzieh Yaeghoobi ◽  
Meysam Afzali ◽  
Neginsadat Amirkhalili ◽  
Majid Mahmoodi ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Side Effects ◽  
Essential Oils ◽  
Ultrasonic Treatment ◽  
Fungal Infections ◽  
Vitro Assay ◽  
Antifungal Activities ◽  
Antifungal Properties

Background and Objectives: Candidiasis and pityriasis versicolor are opportunistic fungal infections that are caused by Candida spp. and Malassezia spp. yeasts. Conventional drugs like azole and amino derivatives are known to treat fungal skin diseases. However, drawbacks like long-term side effects and drug resistance lead to investigate on antifungal properties of phytochemicals as an alternative to available synthetic drugs. Materials and Methods: The herbal nano hydrogel was successfully synthesized from Quince Seed extract followed by ultrasonic treatment and it has been formulated using a mixture of essential oils. We evaluated the antifungal in vitro assay for a mixture of essential oils in combination with herbal nano hydrogel against Candida albicans and Malasezia furfur strains by micro dilution method. Results: The results indicated that essential oils possess antifungal activity with the MIC value of 12.5 and 6.24 mg/ml against C. albicans and M. furfur, respectively. No fungicidal effect was reported for the herbal hydrogel before nanofabrication while it shown some antifungal activity after ultrasonic treatment for 5 and 10 minutes. As anticipated; the antifungal property of essential oil mixture was appreciably improved when it combined with herbal nano hydrogel where the highest level of inhibition was observed at concentration of 3.125 mg/ml for both strains. The loss in biological activity observed when the ultrasonic treatment on herbal nano hydrogel performed for longer time. Conclusion: The proposed plant-based nano formulation shown promising in vitro antifungal activities against C. albicans and M. furfur strains and its antifungal properties were comparable with commercially available agents like clotrimazole. The new formulation expected to be safe with minimum long-term side effects. Further investigations are underway to confirm the safety and the mechanism of the action of this new herbal formulation.

scholarly journals Differential antifungal activity of alfalfa (Medicago santva L.) saponins originated from roots and aerial parts for some ornamental plant pathogens

2013 ◽  
Vol 54 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Alicja Saniewska ◽  
Marian Jurzysta ◽  
Zbigniew Biały
Keyword(s):  
Rhizoctonia Solani ◽  
Botrytis Cinerea ◽  
Plant Pathogens ◽  
Linear Growth ◽  
Inhibitory Effect ◽  
Ornamental Plant ◽  
Mycelium Growth ◽  
Aerial Parts ◽  
Necrotic Spots

The total saponins isolated from aerial parts and roots of alfalfa (<i>Medicago sativa</i> L.) at the concentration of 0,01%, 0,05% and 0,1% showed differential influence on the mycelium growth of <i>Alternaria zinniae, Botrytis cinerea, Botrytis tulipae, Phoma narcissi, Phoma poolensis</i> and <i>Rhizoctonia solani</i>. A higher inhibitory effect on in vitro growth of mycelium of all tested pathogens indicated saponins from roots of alfalfa than from aerial parts. Tested saponins from roots at lhe concentration of 0,1% totally inhibited linear growth of Phoma narcissi, and linear growth of <i>Alternaria zinniae</i> was limited about 67%, Botrytis cinerea about 74%, Botrytis tulipae about 68%, Phoma poolensis about 38%, and <i>Rhizoctonia solani</i> about 74% in comparison to the control. The saponins of alfalfa from roots at the concentration of 0,1% and 0,2% applied as preinoculation sprays evidently inhibited the development of <i>Phoma narcissi</i> on <i>Hippeastrum</i> leaves. This dose of aerial saponins of alfalfa did not effect the development of necrotic spots on the leaves.

scholarly journals Sesquiterpene Lactones from Centaurea achaia, a Greek Endemic Species: Antifungal Activity

2000 ◽  
Vol 55 (7-8) ◽  
pp. 534-539 ◽  
Author(s):  
Helen Skaltsa ◽  
Diamanto Lazari ◽  
Begoña Garcia ◽  
José R. Pedro ◽  
Marina Sokovic ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Endemic Species ◽  
Sesquiterpene Lactones ◽  
Fungal Species ◽  
Dilution Method ◽  
Aerial Parts ◽  
In Vitro Antifungal Activity

Abstract The aerial parts of Centaurea achaia afforded, in addition to several known sesquiterpene lactones and sesquiterpene hydroxyesters, a new germacranolide and a new elemanolide. Their structures were determined as the 8 α-O-(4,5-dihydroxy-tigloyloxy) esters of salonitenolide and of 11,13-dihydromelitensin, respectively. The in vitro antifungal activity of most compounds was tested against nine fungal species using the micro-dilution method. All the tested compounds showed strong antifungal activity.

scholarly journals In vitro screening of fungicides and tannins against fungal pathogens of jujube fruits

2016 ◽  
Vol 27 (2) ◽  
pp. 154-161 ◽  
Author(s):  
MZ Hoque ◽  
AM Akanda ◽  
MIH Miah ◽  
MKA Bhuiyan ◽  
MG Miah ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Fungal Pathogens ◽  
Colletotrichum Gloeosporioides ◽  
Fungal Species ◽  
Plain Water ◽  
Curvularia Lunata ◽  
Mycelium Growth ◽  
Fusarium Semitectum ◽  
Poison Food Technique

The aim of this study to identify the effective fungicides for controlling major leaf and fruit diseases of Jujube caused by different fungal pathogens. An in vitro experiment was undertaken to screen ten fungicides and two Tannins against six important fungal pathogens of Jujube fruits which were Alternaria alternata, Colletotrichum gloeosporioides, Curvularia lunata, Lasiodiplodia theobromae, Fusarium semitectum and Pestalotiopsis palmarum. Fungicides were Conza 5 EC (Hexaconazole), Folicur 250 EC (Tebuconazole) and Potent 250 EC (Propiconazole), Bavistin DF (Carbendazim), Kasumin 2% liquid (Kasugamycin), Rovral 50 WP (Iprodine), Matco 72 WP (Mancozeb 64% + Metalaxyl 8%), Geneb 80 WP (Mancozeb), Emivit 50 WP (Copper oxychloride) and Evavit 80 WG (Sulphur 80 WG). And two tannins were Chestnut Tannin and Quabracho Tannin. Plain water was used as control. Poison food technique was followed to conduct the experiment. Both Tannins did not show satisfactory inhibition of mycelial growth of any of the six fungal pathogens. The effectiveness of fungicides varied greatly with fungal species. The most effective fungicides were Folicur 250 EC followed by Potent 250 EC, Rovral 50 WP, Conza 5 WP and Bavistin DF. Folicur 250 EC caused 89.52% inhibition of mycelium growth of A. alternata and 100% growth inhibition in other five fungi. In addition, Potent 250 EC inhibited growth of L. theobromae, C. lunata, F. semitectum and P. palmarum by 100% and that of C. gloeosporioides by 91.79%. Complete growth inhibition of F. semitectum and P. palmarum was achieved with Bavistin DF. Furthermore, Conza 5 EC caused 100% growth inhibition in C. lunata and more than 90% in L. theobromae and F. semitectum.Progressive Agriculture 27 (2): 154-161, 2016

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