Prevalence, Risk Factors, and Antibiogram of Bacteria Isolated from Milk of Goats with Subclinical Mastitis in Thika East Subcounty, Kenya

A cross-sectional study was carried out to determine the prevalence and risk factors of subclinical mastitis in dairy goats in Thika East Subcounty, Kenya. Further the bacterial pathogens and their antibiogram were investigated. Farm level data on risk factors were obtained from 41 farmers using questionnaires. Milk was obtained from 110 lactating dairy goats and tested for submastitis using California Mastitis Test (CMT). The prevalence of subclinical mastitis at goat level was estimated to be at 50.9% using CMT, out of which 86.5% yielded bacteria on culture. The significant risk factors associated with the occurrence of subclinical mastitis were cleaning schedule (p=0.022, OD=1.047) and parity of the goat (p=0048, OD=1.37). Higher prevalence of subclinical mastitis was observed for goats residing in houses cleaned at least once a fortnight. Does in the first parity were least affected. 169 bacterial isolates were obtained from culture, of which 52 isolates from major classes of isolated bacteria were tested for antibiotic sensitivity to six antibiotics. Fourteen different bacteria were isolated and identified from the milk samples. Coagulase-negative Staphylococci (20.7%), Serratia spp. (19.5%), Citrobacter spp. (16%), Klebsiella spp. (11%), Staphylococcus aureus (10.7%), Enterobacter spp. (6.5%), Escherichia coli (5.9%), Proteus spp. (3%), Corynebacterium spp. (1.8%), Morganella spp. (1.8%), Streptococcus spp. (1.2%), Providencia spp. (0.6%), Micrococcus spp. (0.6%), and Staphylococcus intermedius (0.6%) were isolated and identified from the samples. All the isolates were resistant to Penicillin G, while 98% of the isolates were sensitive to Streptomycin. In conclusion, the study showed that a large proportion of goats were affected by subclinical mastitis, with the main bacteria being Staphylococci spp. and coliforms, and that most of the tested antibiotics can be used in the treatment of mastitis. Farmers need to be trained on improved control of mastitis through adoption of good dairy husbandry and milking practices.

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Epidemiological studies on subclinical mastitis in dairy goats in northern regions of Bangladesh

Bangladesh Journal of Livestock Research ◽

10.3329/bjlr.v19i1-2.26433 ◽

2016 ◽

Vol 19 (1-2) ◽

pp. 112-122 ◽

Cited By ~ 3

Author(s):

MIA Begum ◽

MS Hossain ◽

M Ershaduzzaman ◽

MS Alam

Keyword(s):

Subclinical Mastitis ◽

Epidemiological Studies ◽

Cross Sectional Study ◽

Housing System ◽

Dairy Goats ◽

Coagulase Negative Staphylococci ◽

Cross Sectional ◽

Milk Samples ◽

California Mastitis Test ◽

Mastitic Milk

A cross sectional study was carried out from June, 2008 to December, 2010 to estimate the prevalence of subclinical mastitis and to determine the responsible bacterial pathogens in lactating dairy goats in northern districts of Bangladesh. A total of 292 goats and 584 udder halves milk samples were screened using California Mastitis Test (CMT). Prevalence of subclinical mastitis at goat level was 56.2% (164/292), and in udder half level it was 33.9% (198/584). The subclinical mastitis prevalence at goat level was high (71.6%) in Jamnapari goats as compared to Black Bengal goats (50.2%), whereas at the udder half level, subclinical mastitis prevalence was 45.1% and 29.6% in Jamnapari and Black Bengal goats, respectively. The pathogens isolated from subclinical mastitic milk samples were coagulase negative Staphylococci, Coliforms, Streptococcus spp., Staphylococcus aureus, Micrococcus spp., Pseudomonas aeruginosa and Bacillus cereus. Among these, the most frequent isolates were coagulase negative Staphylococci (28.8%), Coliforms (22.7%) and Streptococcus spp (15.2%). Out of four potential host related risk factors considered, litter size and body condition of goats were found to influence the prevalence rate of subclinical mastitis in goat significantly (p< 0.05). On the other hand, the subclinical mastitis was very significantly (p=0.0001) associated with the housing system of goats, i.e., goats reared in raised floor had a low subclinical mastitis infection rate (35.8%) as compared to reared in earth floor (62.2%). Antibiogram studies were also performed for the bacterial isolates and Gentamicin was found to be the most effective drug.Bangladesh J. of Livestock Res. 19(1-2): 112-122, Jan-Dec 2012

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Assessment of sub-clinical mastitis and its associated risk factors in dairy livestock of Lamjung, Nepal

International Journal of Infection and Microbiology ◽

10.3126/ijim.v2i2.8322 ◽

2013 ◽

Vol 2 (2) ◽

pp. 49-54 ◽

Cited By ~ 2

Author(s):

T Khanal ◽

A Pandit

Keyword(s):

Risk Factors ◽

Management Practices ◽

Subclinical Mastitis ◽

Clinical Mastitis ◽

Cross Sectional Study ◽

Biochemical Tests ◽

Cross Sectional ◽

California Mastitis Test ◽

Dairy Animals ◽

Associated Risk Factors

INTRODUCTION: Mastitis is one among the top three threats faced by dairy farmers. The study was carried out to assess sub-clinical mastitis, management practices and associated risk factors for mastitis. MATERIALS AND METHODS: A cross sectional study was conducted in Chandreshwor and Archalbot VDCs of Lamjung district taking 63 dairy livestock randomly each from a herd along with questionnaire survey to respective owner. Tem ml of milk sample from each quarter was taken in a sterilized syringe for further laboratory investigation. California Mastitis Test (CMT) was performed at farmer’s shed. Organisms were identified based on colony characteristics, Gram's staining and various biochemical tests. RESULTS: On CMT, subclinical mastitis was 46.1% (n=29) and 30.15% (n=76) on animal and quarter basis respectively, however, culture showed, 28.6% and 24.2%. Streptococcal mastitis was the commonest (11.1%) followed by coliform (9.5%) and staphylococcal (7.9%). Mastitis was highest in left fore quarter (34.92%) followed by left hind (31.76%), right hind (28.57%) and right fore (25.39%). Coliform & Staphylococcal mastitis was highest in left fore and right hind quarter respectively. Most of dairy animals (86%) were on zero grazing, 30% (n=19) of the farmers had forage trees and only 29% (n=18) had known about subclinical mastitis. The average milk production was 3.5 ±1.47 liters. Subclinical mastitis was associated significantly (p<0.01) with livestock yielding more than 3 lt per lactation. CONCLUSIONS: There was high prevalence of subclinical mastitis in dairy livestock at Lamjung due to poor management, unhygienic shed, and little knowledge on subclinical mastitis. DOI: http://dx.doi.org/10.3126/ijim.v2i2.8322 Int J Infect Microbiol 2013;2(2):49-54

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β-lactam resistance in bacteria associated with subclinical mastitis in goats in Thika Subcounty, Kenya

Veterinary World ◽

10.14202/vetworld.2020.1448-1456 ◽

2020 ◽

Vol 13 (7) ◽

pp. 1448-1456

Author(s):

Irene Mkavi Okoko ◽

Naomi Maina ◽

Daniel Kiboi ◽

John Kagira

Keyword(s):

Risk Factors ◽

Antibiotic Resistance ◽

Subclinical Mastitis ◽

Penicillin G ◽

Confirmatory Test ◽

Diffusion Method ◽

Dairy Goats ◽

Isolation And Identification ◽

Factors Associated ◽

Synergy Test

Aim: This study determined the resistance pattern to β-lactam antibiotics of bacteria isolated from goats with subclinical mastitis in Thika subcounty, Kenya. We also administered a questionnaire to assess the risk factors associated with the occurrence of resistance to commonly used antibiotics. Materials and Methods: We collected milk samples from 110 lactating dairy goats in Thika subcounty to screen for subclinical mastitis using the California mastitis test. Bacterial isolation and identification were performed according to colony morphology, the hemolytic pattern on sheep blood agar, lactose fermentation on MacConkey plates, Gram staining, and standard biochemical tests. The antibiotic susceptibility of the isolates was determined by the agar disk diffusion method using penicillin G, cephalexin, cefoxitin, and cefotaxime antibiotic disks. The double-disk synergy test using amoxicillin-clavulanic acid was employed as a confirmatory test for extended-spectrum β-lactamase (ESBL) production. Fisher's exact test was used to determine the risk factors associated with the occurrence of antibiotic resistance (p≤0.05 was considered significant). Results: Of the 110 dairy goats sampled, 72.7% (80) were positive for subclinical mastitis. Isolation and identification of the bacteria from the positive samples yielded 149 bacteria isolates, including Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter spp., Yersinia spp., coagulase-negative staphylococci, and Escherichia coli. A high percentage (76.5%, 114/149) of the bacterial isolates was resistant to at least one of the tested antibiotics. At least 56/106 isolates (52.8%) showing cross-resistance to the β-lactam antibiotics were resistant to all four of the tested antibiotics, while only one isolate was resistant to three antibiotics (penicillin G, cephalexin, and cefoxitin). The double-disk synergy test confirmed that none of the isolates possessed ESBLs. Pre- and post-milking practices (p=0.0336) were found to be significantly associated with the occurrence of antibiotic resistance. Conclusion: A large proportion of the goats in our study cohort were infected with β-lactam-resistant bacteria associated with subclinical mastitis. Because the identified bacteria are of zoonotic importance, further studies should be undertaken to determine the transmission dynamics between humans and livestock and to identify novel intervention strategies.

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A study on prevalence of chronic kidney disease and its risk factors among adults in selected slums of Chennai

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20190059 ◽

2019 ◽

Vol 6 (2) ◽

pp. 504

Author(s):

K. Kokila ◽

K. Chellavel Ganapathi

Keyword(s):

Risk Factors ◽

Chronic Kidney Disease ◽

Kidney Disease ◽

Significant Risk ◽

Significant Risk Factor ◽

Cross Sectional Study ◽

Uncontrolled Hypertension ◽

Adult Males ◽

Cross Sectional ◽

Slum Population

Background: Chronic kidney disease (CKD) is a silent epidemic. The true prevalence of CKD with in a population are very difficult to estimate, since early to moderate CKD were usually asymptomatic. ESRD in the consequence of CKD is one of the most expensive diseases to treat.Only way is to prevent it and Hence this was conducted to estimate the prevalence of CKD and its associated risk factors among adults in selected slums of Chennai.Methods: This is a cross sectional study done from May 2013 to June 2014 in selected slums of Chennai. The study population includes adult males and females. Multi stage sampling method used. Sample size covered was 400. A semi structured questionnaire used as tool. Based on the serum creatinine eGFR calculated using modified MDRD study equation and CKD prevalence was identified. The data was entered in MS excel and analyzed using SPSS version 21.Results: Total of 400 participants involved. Females were predominant in the study. Among them, 67 (16.8%) were diagnosed as CKD patients and Stage 1, 4.0% had stage 2, 3.6% had stage 3, 1.3% had stage 4 and 0.5% had stage 5 at the time of diagnosis.Conclusions: This study revealed prevalence of CKD among slum population was 16.8%. This study showed that old age, uncontrolled hypertension, uneducated, Diabetes with poor control, overuse of analgesics, H/o smoking, obesity, alcoholism, passive smoking, family H/o CKD and proteinuria were significant risk factor for CKD among study subjects. Slum population with risk factors should be regularly screened for CKD.

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Risk Factors for Infection with Soil Transmitted Helminths, Cryptosporidium spp., and Giardia duodenalis in Children Enrolled in Preschools in Kafue District, Zambia

Epidemiology Research International ◽

10.1155/2015/906520 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Joyce Siwila ◽

Annette Olsen

Keyword(s):

Risk Factors ◽

Longitudinal Study ◽

Significant Risk ◽

Giardia Duodenalis ◽

Significant Risk Factor ◽

Cross Sectional Study ◽

Parasitic Infections ◽

Cross Sectional ◽

Low Socioeconomic ◽

Soil Transmitted Helminths

Intestinal parasitic infections are common among children worldwide. This study was aimed at determining risk factors for infection with soil transmitted helminths, Cryptosporidium spp., and Giardia duodenalis, among children in preschools. The study was in two parts: a cross-sectional study in which data were collected from 403 children from 10 preschools and a longitudinal study in which 100 children from four preschools from the previous 10 were selected. Prevalence of Ascaris lumbricoides was 12.0%, while that of hookworm was 8.3%. Overall prevalence of Cryptosporidium and G. duodenalis was 28.0% and 29.0%, respectively. Low education level of parent/guardian was a significant risk factor for A. lumbricoides (OR = 2.35, 95% CI: 1.02–5.41; P=0.04), while roofing types other than corrugated iron sheets were found to be protective for G. duodenalis infection in both bivariate and multivariate analyses (multivariate: OR = 0.67, 95% CI: 0.45–0.99; P=0.04). Low socioeconomic level was found to be protective for Cryptosporidium spp. infection in multivariate analysis (OR = 0.59, 95% CI: 0.35–0.99; P=0.04). In the longitudinal study, none of the factors were associated with either infection. These findings may have implications for other preschools in other districts in Zambia.

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Socioeconomic, Psychiatric and Materiality Determinants and Risk of Postpartum Depression in Border City of Ilam, Western Iran

Depression Research and Treatment ◽

10.1155/2013/653471 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Pegah Taherifard ◽

Ali Delpisheh ◽

Ramin Shirali ◽

Abdorrahim Afkhamzadeh ◽

Yousef Veisani

Keyword(s):

Risk Factors ◽

Postpartum Depression ◽

Low Socioeconomic Status ◽

Data Gathering ◽

Significant Risk ◽

Cross Sectional Study ◽

Cross Sectional ◽

Western Iran ◽

Border City ◽

Significant Difference

Background. Postpartum depression (PPD) is considered as one of the mood disturbances occurring during 2-3 months after delivery. The present study aimed to determine the prevalence of PPD and its associated risk factors in border city of Ilam, western Iran.Methods. Through a descriptive cross-sectional study in 2011, overall, 197 women who attended Obstetrics & Gynecology clinics postpartumly in the border city of Ilam, western Iran, were randomly recruited. A standard questionnaire that was completed by a trained midwife through face to face interviews was used for data gathering.Results. Mean age ± standard deviations was 27.9 ± 5.2 years. Prevalence of PPD was estimated to be 34.8% (95% CI: 27.7–41.7). A significant difference was observed among depression scores before and after delivery (P≤0.001). Type of delivery (P=0.044), low socioeconomic status (P=0.011), and women having low educational level (P=0.009) were the most important significant risk factors associated with PPD. The regression analysis showed that employed mothers compared to housekeepers were more at risk for PPD (adjusted OR = 2.01, 95% CI: 1.22–2.28,P=0.003).Conclusions. Prevalence of PPD in western Iran was slightly higher than the corresponding rate from either national or international reports.

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Frequency and risk factors of internet addiction in medical students: a cross-sectional study

Journal of the Pakistan Medical Association ◽

10.47391/jpma.451 ◽

2021 ◽

pp. 1-11

Author(s):

Amina Ehsan ◽

Farwa Iqbal ◽

Muhammad Azeem Rao

Keyword(s):

Risk Factors ◽

Medical Students ◽

Internet Addiction ◽

Significant Risk ◽

Sampling Technique ◽

Total Sample ◽

Cross Sectional Study ◽

Sectional Study ◽

Online Relationships ◽

Cross Sectional

Abstract Objectives: The objectives of the study were to find out the prevalence of internet addiction and to determine the risk factors associated with internet addiction in medical students. Methods: This descriptive cross-sectional study was carried out at Rawalpindi Medical University, Rawalpindi from July 2018 to August 2019. Our study population included medical students from all the years of MBBS aged 18-25 years, who have been using the internet for the past two years or more. A Stratified random sampling technique was used. Seventy-six self-administered questionnaires were distributed in each year. Data was entered and analyzed using the Statistical Package for Social Sciences (SPSS version 25). Results: Out of the total sample of 380, the prevalence came out to be 90% (n=342). Two hundred and twenty-two 222 (58.4%) had mild, 115 (30.3%) had moderate, six (1.6%) had severe and 37 (9.7%) had no addiction. Out of the risk factors online friendships (p= 0.007), online relationships (P=0.035), online chatting (p= 0.009), online shopping (p= 0.009), online games (p=0.023) and online series/movies (p< 0.000) were statistically significant. Conclusion: The prevalence of internet addiction is high among medical students, which is mostly of a mild type. Internet addiction is more common in males and non-hostel residents. Other significant risk factors are online relationships, online gaming, and online series/movies. Keywords: Prevalence, internet, medical students, risk factors. Continuous...

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Incidence, possible risk factors and therapies for pseudopregnancy on Dutch dairy goat farms: a cross-sectional study

Veterinary Record ◽

10.1136/vr.105346 ◽

2019 ◽

Vol 184 (25) ◽

pp. 770-770 ◽

Cited By ~ 1

Author(s):

René Van den Brom ◽

Rianne Klerx ◽

Piet Vellema ◽

Karianne Lievaart-Peterson ◽

Jan Willem Hesselink ◽

...

Keyword(s):

Risk Factors ◽

Response Rate ◽

Cross Sectional Study ◽

Annual Incidence ◽

Dairy Goat ◽

Induction Treatment ◽

Sectional Study ◽

Dairy Goats ◽

Cross Sectional ◽

Treatment Measures

Pseudopregnancy is a frequently diagnosed reproductive disorder in (dairy) goats. This cross-sectional study evaluates the incidence, possible risk factors and therapies for pseudopregnancy on Dutch dairy goat farms. Two questionnaires, one for farmers and one for veterinarians, were designed and included questions about general farm demographics, breeding management, hormonal oestrous induction, treatment, measures for reduction and stress moments in dairy goats in the period June 1, 2016–May 31, 2017. In total, 43 farmers (21.5 per cent response rate) and 27 veterinarians (22.5 per cent response rate) completed the questionnaire. The annual incidence of pseudopregnancy varied between 1 and 54 per cent per farm, with a mean annual incidence of 17 per cent (95 per cent CI 0.14 to 0.21). In this study, we found a significant association between incidence of pseudopregnancy and a higher percentage of goats with an extended lactation (p<0.0001) and between incidence of pseudopregnancy and the number of ultrasound examinations per year (p<0.0001). The recommended therapy in literature consists of two administrations of prostaglandins. This was only correctly applied by 10 per cent of the farms. On 52 per cent of the farms, an overdose was used comparing to the recommended dose in literature.

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Characteristics and risk factors of Plasmodium falciparum malaria in Eastern and Central Nepal

Kathmandu University Medical Journal ◽

10.3126/kumj.v7i4.2758 ◽

1970 ◽

Vol 7 (4) ◽

pp. 378-382 ◽

Cited By ~ 3

Author(s):

MR Banjara ◽

W Sirawaraporn ◽

S Petmitr ◽

M Imwong ◽

AB Joshi ◽

...

Keyword(s):

Risk Factors ◽

Falciparum Malaria ◽

Central Region ◽

Significant Risk ◽

Cross Sectional Study ◽

Eastern Region ◽

Limited Information ◽

Laboratory Assessment ◽

Cross Sectional ◽

Central Nepal

Background: Very limited information is available on epidemiology of falciparum malaria in Nepal. Such information is very important for malaria control programmes. It is believed that malaria in Eastern region is imported from border districts of India and local transmission follows whereas it is indigenous in Central region. Therefore, the characteristics and risk factors of malaria are believed to be different in Eastern and Central Nepal. Objective: The objective of the study is to describe and compare the characteristics and risk factors of falciparum malaria in Eastern and Central Nepal. Materials and methods: This cross-sectional study was conducted in falciparum malaria endemic districts of Eastern and Central Nepal, during the period 2007 to 2008. We identified and collected information from 106 patients (62 from Eastern and 44 from Central region). Patient examination, clinical and laboratory assessment were done and patients were interviewed using structured questionnaire for malaria related characteristics, risk factors and behaviours. Results: There were significant differences in risk factors and characteristics of falciparum malaria in the Central than the Eastern region. In the Central region, male, illiteracy and thatched roof hut were significant risk factors of falciparum malaria patients as compared to the Eastern region. Visits outside within one months, previous malaria within three months, taking antimalarial before confirmatory diagnosis were significantly higher in patients of the Eastern region as compared to the Central region. Conclusion: Falciparum malaria in Nepal should not be seen as similar entity, and different strategies for prevention and control is needed for its diverse characteristics and endemicity. Key words: Eastern and Central Nepal; falciparum malaria; risk factors DOI: 10.3126/kumj.v7i4.2758 Kathmandu University Medical Journal (2009) Vol.7, No.4 Issue 28, 378-382

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The Prevalence and Associations of Peripheral Retinopathy: Baseline Study of Guangzhou Office Computer Workers

Journal of Ophthalmology ◽

10.1155/2018/2358690 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6

Author(s):

Ting Zhang ◽

Yajing Zuo ◽

Yantao Wei ◽

Wenbin Huang ◽

Xuezhi Zhou ◽

...

Keyword(s):

Risk Factors ◽

Significant Risk ◽

Cross Sectional Study ◽

Cross Sectional ◽

Computer Workers ◽

Lattice Degeneration ◽

Associated Risk Factors ◽

Retinal Hole ◽

Slit Lamp Microscopy ◽

Chorioretinal Degeneration

Purpose. To determine the prevalence of peripheral retinopathy and its associated risk factors among a sample of Guangzhou office computer workers. Methods. A cross-sectional study of Guangzhou Chinese computer workstations and operators in different departments and units of the Guangzhou Power Supply Bureau, China, in 2016. Peripheral retinopathy was recorded and analyzed using a scanning laser ophthalmoscope (SLO; Optos, Daytona, United Kingdom) and slit-lamp microscopy combined with a three-mirror contact lens. Results. The 1934 eyes of 967 subjects (513 females and 454 males) were included in this study. In total, 79.1% of the eyes were myopic in workers aged 20–29 years, 72.9% in workers aged 30–39 years, 62.2% in workers aged 40–49 years, and 43.4% in workers aged 50–59 years (p<0.001). Most eyes had optic nerve crescents (81.3%). Various peripheral degenerations were found: 7 eyes (0.4%) had microcystoid degeneration, 40 (2.1%) had peripheral pigmentary degeneration, 87 (4.5%) had lattice degeneration, and 4 (0.2%) had snail-track degeneration. Nineteen (1.0%) eyes had paving-stone degeneration, 11 (0.6%) eyes had a retinal hole or tear, and 16 (0.8%) eyes had chorioretinal degeneration. Multivariate regression confirmed that greater axial length (OR: 1.18 (1.03, 1.35), p=0.012) and more serious spherical equivalent (OR: 0.82 (0.77, 0.88), p<0.001) were significant risk factors for peripheral retinal changes. Conclusion. Peripheral retinal degenerative changes were found in a larger proportion of younger computer workers than older ones. Myopia is occurring in younger and younger people, accompanied by peripheral retinal degeneration.

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