scholarly journals Vancomycin-Resistant Enterococci and Its Associated Risk Factors among HIV-Positive and -Negative Clients Attending Dessie Referral Hospital, Northeast Ethiopia

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Seid Ali ◽  
Martha Alemayehu ◽  
Mulat Dagnew ◽  
Teklay Gebrecherkos
Keyword(s):  
Risk Factors ◽  
Multidrug Resistant ◽  
Referral Hospital ◽  
Health Concern ◽  
Diffusion Method ◽  
Hiv Positive ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Associated Risk Factors ◽  
Dessie Referral Hospital

Background. Enterococci are becoming the most important public health concern and emerging as multidrug-resistant organisms around the world including Africa particularly in Ethiopia where there is a lack of availability of effective antimicrobial drugs. However, there is a paucity of data on the prevalence and associated risk factors of vancomycin-resistant enterococci in Ethiopia.Objective. This study was aimed to assess the prevalence of vancomycin-resistant enterococci and its associated risk factors among HIV-positive and -negative clients.Methods. A comparative cross-sectional study was conducted from February to May, 2017, on 300 participants at Dessie Referral Hospital. Data were gathered using a pretested structured questionnaire, stool samples were collected and inoculated on to bile esculin agar, and presumptive colonies were inoculated in brain-heart infusion broth containing 6.5% NaCl for selective identification of enterococci. Antibiotic susceptibility tests were done using the Kirby–Bauer disk diffusion method. Data were analyzed using SPSS version 22 software package.Results. A total of 300 study participants were enrolled in this study, of which 57.7% were females with a mean age of 34.4, a range of 19–73 years. The overall prevalence of enterococci was 37.3%. The prevalence of VRE was 6.3%. From all isolates, the prevalence of VRE among HIV-positive and -negative clients was 5.9% and 7.4%, respectively. Resistance gentamicin, ampicillin, penicillin, and erythromycin was 37.5%, 34.8%, 34.8%, and 22.3%, respectively. Prevalence of multidrug resistance was (29.5%). Being low in hemoglobin content was significantly associated with VRE.Conclusion. The high prevalence of VRE and multidrug-resistant enterococci in this study signals the emergence of VRE. Detection of VRE in this study indicates decreased antibiotic treatment options of multidrug-resistant enterococci. Therefore, there should be a need to perform continuous surveillance, rational use of antibiotics, and more detailed study using phenotypic and genotypic methods.

scholarly journals Prevalence, Antimicrobial Susceptibility Patterns, and Risk Factors Associated with Enterococci among Pediatric Patients at Dessie Referral Hospital, Northeastern Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Admasu Abera ◽  
Mihret Tilahun ◽  
Saba Gebremichael Tekele ◽  
Melaku Ashagrie Belete
Keyword(s):  
Risk Factors ◽  
Pediatric Patients ◽  
Multidrug Resistant ◽  
Body Fluids ◽  
Referral Hospital ◽  
Health Concern ◽  
Resistance Pattern ◽  
History Of ◽  
Associated Risk Factors ◽  
Dessie Referral Hospital

Background. Enterococcus species, which is previously considered as medically not important, now becomes one of the leading causes of nosocomial infections. Even though it becomes the most public health concern and emerging multidrug-resistant pathogen, there is no enough data in the study area. Objective. To determine the prevalence, antimicrobial resistance pattern, and associated risk factors of enterococci infection in pediatric patients. Methods. A hospital-based cross-sectional study was conducted from February to May 2019 at Dessie Referral Hospital, Northeastern Ethiopia. A total of 403 pediatric patients were included in the study, and a pretested structured questionnaire was used to collect sociodemographic and risk factor-related data. Clinical samples such as urine, blood, wound swabs, discharges, and other body fluids were collected aseptically and inoculated on to Bile Esculin Azide Agar, and colony characteristics, Gram stain, catalase, salt, and temperature tolerance tests were employed for bacterial identification. Antimicrobial sensitivity tests were performed using the modified Kirby-Bauer disk diffusion method. Data was entered into SPSS software version 25 and descriptive statistics; bivariate and multivariate logistic regression analyses were performed. In all cases, a P value ≤ 0.05 with corresponding 95% confidence interval were considered as statistically significant. Result. The overall prevalence of enterococci was 2.7% (11/403). Of which, the highest number of enterococci infection was recovered from urine sample (54.5%) followed by blood (27.3%), wound swab (9.1%), and other body fluids (9%). The overall multidrug resistance rate was 54.5%. Higher drug resistance pattern was observed against tetracycline, chloramphenicol, and amoxicillin/clavulanate. Having history of invasive procedure ( P < 0.001 ), chronic illness ( P < 0.001 ) and previous admission history of the children ( P < 0.001 ) were statistically significant associated risk factors for pediatrics enterococci infection. Conclusion. The prevalence of enterococci from pediatric patients in this study was relatively low compared to other studies. Significant rates of MDR and VRE were identified, and the risk of infection became high when children had a history of different chronic illnesses and history of admission and underwent invasive treatment procedures. Therefore, efforts should be made to prevent enterococci infections and spread of multidrug-resistant enterococci.

scholarly journals Prevalence, Antibiotic Susceptibility Profile, and Associated Risk Factors of Salmonella Isolate among Diarrheal Patients Visiting Dessie Referral Hospital, Northeast Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Samuel Chane Teferi
Keyword(s):  
Risk Factors ◽  
Antibiotic Susceptibility ◽  
Public Health Problem ◽  
Sensitivity Test ◽  
Referral Hospital ◽  
Diffusion Method ◽  
Old Patients ◽  
Northeast Ethiopia ◽  
Associated Risk Factors ◽  
Dessie Referral Hospital

Salmonellosis remains an important public health problem worldwide, particularly in the developing countries such as Ethiopia. A cross-sectional study was conducted to determine the prevalence, antibiotic susceptibility profile, and associated risk factors of Salmonella isolate among diarrheal patients who were visiting Dessie Referral Hospital, Dessie, Northeast Ethiopia, from November 2016 to January 2017. 384 stool samples were collected using sterile stool cups. Out of these, 20 (5.21%) were found to be positive for Salmonella species. The distribution of positive samples among the three age groups indicated that Salmonella species were predominantly prevalent in the age group of three months (0.25 years) to 4 years old patients. Abdominal pain, vomiting, watery consistency of stool, and 1–5 days of diarrhea were the clinical features that were significantly associated with salmonellosis. Eating raw vegetables and fruits, consumption of street-vended foods, cohabitation of animals, using water from the unprotected source, absence of latrine, and consumption of raw products of animals such as eggs and raw milk were the risk factors that were significantly associated with the prevalence of Salmonella species. The antibiotic sensitivity test was performed for the isolated Salmonella species against 5 currently recommended antibiotics. The antimicrobial sensitivity study carried out using the Kirby–Bauer disk diffusion method showed that 100% of Salmonella isolates were sensitive to ciprofloxacin and 80% sensitive to nalidixic acid, respectively. Among them, twenty isolated Salmonella species resistant to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole were 100%, 85%, and 80%, respectively. This study revealed that Salmonella species were prevalent among diarrheal patients who were visiting Dessie Referral Hospital, and therefore, routine diagnosis of patients with diarrhea cases is required, and drugs must be prescribed after performing the sensitivity test, or checking the updated information on the local antibiotics pattern is always necessary.

scholarly journals Frequency of vancomycin-resistant Enterococci isolated from blood cultures from 2008 to 2010

2011 ◽  
Vol 64 (9-10) ◽  
pp. 481-485 ◽  
Author(s):  
Mira Mihajlovic-Ukropina ◽  
Deana Medic ◽  
Zora Jelesic ◽  
Vera Gusman ◽  
Biljana Milosavljevic
Keyword(s):  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Blood Cultures ◽  
Hospitalized Patients ◽  
Diffusion Method ◽  
Aminoglycoside Resistance ◽  
Vancomycin Resistant Enterococci ◽  
Enterococcus Spp ◽  
Vancomycin Resistant ◽  
High Level

Introduction. Enterococci are important hospital-acquired pathogens. The most commonly isolated species of the genus, Enterococcus faecalis and Enterococcus faecium are the third to fourth-most prevalent nosocomial pathogens worldwide. The aim of this study was to determine the frequency of resistance to vancomycin and other antimicrobial agents of Enterococcus spp strains isolated from blood cultures of hospitalized patients. Material and methods. During the three-year period, from 2008 to 2010, 132 strains of Enterococcus spp isolated from blood cultures of hospitalized patients were tested for their susceptibility to ampicillin, vancomycin, gentamycin (high-level resistance), erythromycin, chloramphenicol, teicoplanin, ciprofloxacin by disc diffusion method according to the Clinical and Laboratory Standards Institute recommendations. Susceptibility of vancomycin resistant E. faecium to the same antibiotics and to linezolid, quinopristin/dalfopristin and tigecyclin was determined using VITEK system. Results and discussion. Resistance to vancomycin was detected in 21 (15.9%) Enterococcus spp strains. The percentage of resistance to other antimicrobial agents varied from 23.1% for chloramphenicol to 81.3% for ciproflxacin. All vancomycin resistant enterococci were identified as E. faecium and belonged to phenotype VanA. The resistance to other antibiotics was very high, except for linezolid and quinopristin/dalfopristin (4.7%). The high-level aminoglycoside resistance was 87.6% for gentamycin and 95.2% for streptomycin. All isolates were resistant to ampicillin, teicoplanin and ciprofloxacin. Conclusion. The detected high frequency of multidrug-resistant isolates among vancomycin resistant enterococci is of great importance and suggests the need for further monitoring of susceptibility in order to take adequate measures to prevent and control spreading of resistant strains.

scholarly journals Vancomycin-resistant enterococci and coagulase-negative staphylococci prevalence among patients attending at Felege Hiwot Comprehensive Specialized Hospital, Bahir Dar, Ethiopia

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249823
Author(s):  
Degu Ashagrie ◽  
Chalachew Genet ◽  
Bayeh Abera
Keyword(s):  
Venous Blood ◽  
Multidrug Resistant ◽  
Vancomycin Resistance ◽  
World Health ◽  
Diffusion Method ◽  
P Value ◽  
Coagulase Negative Staphylococci ◽  
Vancomycin Resistant Enterococci ◽  
Specialized Hospital ◽  
Vancomycin Resistant

Background Vancomycin resistant enterococci (VRE) and vancomycin resistance coagulase negative staphylococci (VRCoNS) are common pathogens causing difficult to treat health care associated infections (HAI). Hence, the World Health Organization listed VRE as one of the high priority pathogens for new antibiotic discovery and antimicrobial resistance surveillance. Despite this, data on the prevalence of VRE and VRCoNS in Ethiopia is scarce. Thus, the present study determined prevalence of VRE and VRCoNS among patients attending Felege-Hiwot comprehensive specialized hospital, Ethiopia. Methods A hospital based cross-sectional study was conducted on 384 patients selected conveniently from February to March 2020. Data on demographic and clinical variables were collected using a structured questionnaire by face-to-face interview. Simultaneously urine, venous blood and wound swab were collected and processed following standard bacteriological technique. Antimicrobial susceptibility test was performed by minimum inhibitory concentration method using E-test for vancomycin and Kirby-Bauer disc diffusion method for other classes of antibiotics. Data was entered and analyzed using SPSS version 23. Logistic regression was performed to identify factors associated with VRE infection. P. value < 0.05 was considered as statistically significant. Results The prevalence of enterococci and CoNS were 6.8% and 12% respectively. The prevalence of VRE was 34.61% (9/26), while all CoNS (46 isolates) were susceptible to vancomycin. The majority (66.7%) of VRE was isolated from blood samples. Furthermore all VRE (100%), 58.8% of vancomycin susceptible enterococci and 45.7% of CoNS were multidrug resistant (MDR). Having educational level of secondary school and below (AOR = 12.80, CI = 1.149–142.5), previous exposure to catheterization (AOR = 56.0, CI = 4.331–724.0) and previous antibiotic use practice (AOR = 26.25, CI = 3.041–226.2) were a significant associated explanatory factor for VRE infection. Conclusions The prevalence of vancomycin resistance enterococci, which is also multidrug resistant, was significantly high. Though no vancomycin resistance CoNS detected, the MDR level of CoNS was high. Thus to limit enterococci and CoNS infections and MDR development, focused infection prevention measures should be implemented.

scholarly journals Prevalence of HIV and Associated Risk Factors Among Infants Born to HIV Positive Mothers Attending Entebbe Regional Referral Hospital.

2021 ◽  
Author(s):  
◽  
KEEFA WAMALA ◽  
Ronald Nuwamanya ◽  
Moses Muwanga
Keyword(s):  
Risk Factors ◽  
Referral Hospital ◽  
Hiv Positive ◽  
People Living With Hiv ◽  
Regional Referral Hospital ◽  
Hiv Exposed Infants ◽  
Hiv Positive Mothers ◽  
Hiv Exposed ◽  
Associated Risk Factors ◽  
Mother To Child

Background: Uganda has an estimated 1.4 million people living with HIV with about 52,000 infections occurring every year. In 2018, 160,000 children were reported to have become infected with HIV. Globally, HIV exposed infants have delayed access to Early Infant Diagnosis (EID) of HIV, thus hampering efforts towards zero new infections. In Uganda, the prevalence of HIV among infants is not recorded, peak mortality for infants born with HIV occurs between 2 and 3 months of age. Vertical transmission of HIV from mother to child is the second commonest route of transmission of HIV in Uganda accounting for 18% of all new infections. This study assessed the prevalence of HIV and associated risk factors among infants born to HIV positive mothers attending Entebbe regional referral hospital. Methodology: a cross-sectional study was conducted at a paediatric ward and Mother-Child Health (MCH) General Department of Entebbe Regional Referral Hospital. The study included 78 HIV-exposed infants whose blood samples were collected and analyzed to know their HIV status and data about risk factors was also collected. Data were collected using questionnaires from mothers. Data were then entered into an Excel spreadsheet and analysed by SPSS Version 20. Results: The prevalence of HIV infection among HIV exposed infants is 5.1%.  Delay in child diagnosis, breastfeeding was the factors that increased the risk of mother-to-child transmission of HIV in this study. Conclusion and recommendations: Having such a significant figure greater than the proposed WHO recommendation of less than 5% new infections in infants in the era of the world’s pledge to eliminate MTCT of HIV is unbearable therefore, interventions need to be done to lower this prevalence

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