scholarly journals Evaluation of the Biological Effects of Lyophilized Hydrophilic Extract of Rhus coriaria on Myeloperoxidase (MPO) Activity, Wound Healing, and Microbial Infections of Skin Wound Tissues

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Sami A. Gabr ◽  
Ahmad H. Alghadir
Keyword(s):  
Wound Healing ◽  
Enzyme Activity ◽  
Scar Formation ◽  
Wound Contraction ◽  
Collagen Deposition ◽  
Staph Aureus ◽  
Microbial Infections ◽  
Rhus Coriaria ◽  
Anti Inflammatory ◽  
Wound Healing Activity

Impaired wound healing was mainly associated with severe microbial infections which significantly affect diagnostic and therapeutic strategies. Thus, in this study, the potential wound healing activity, anti-inflammatory, and antimicrobial activity of an aqueous extract of Rhus coriaria extract (AERc) were evaluated by wound contraction, scar formation, period of epithelization, MPO enzyme activity, collagenase-2 (MMP-8), hydroxyproline (HPX), and collagen deposition as markers of wound healing at different days of postwound. Phytoconstituents, microbial activity, and fibrogenic markers were screened by HPLC, disc-diffusion, and colorimetric assays. The animals were treated with Rhus coriaria extract (AERc) concentrations at doses of 5 mg.kg−1and 10 mg.kg−1, respectively. On days 6 and 9, the AERc-treated animals at doses of 5 mg.mL−1 and 10 mg.mL−1 exhibited a significant reduction in the wound area, increased deposition of collagen, HPX, and reduction in MMP-8, and MPO enzyme activity when compared with controls. Scar formation and epithelization were completed in 10 days compared to controls. In addition, in wounds infected separately with Staph. aureus or P. aeruginosa, the AERc extract significantly improved wound contraction, deposition of collagen, and HPx and reduced MMP-8 and MPO concentrations, with complete epithelization of wounds in 10-13 days compared to the saline-treated group. Hydrolyzable tannins, gallic acid, quercetin, and myricetin were the most common active components of AERc. In vitro, the AERc and its components were effective against a set of microbes especially Staph. aureus, P. aeruginosa, and Staph. aureus (MRSA). In conclusion, the results showed that antimicrobial, anti-inflammatory, and antioxidant activity of Rhus coriaria extract suggested its importance as a target for formulation of novel drugs against many microbial infections with minimal side effects and could play a good potential role in accelerating wound healing activity via promoting myofibroblast activity, increase of hydroxyproline and collagen deposition, and regulation of MMP-8 and MPO enzyme activities.

IN VIVO EVALUATION OF WOUND HEALING AND ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF ROOTS OF CENTAUREA AFRICANA (L.) IN TOPICAL FORMULATION

2016 ◽  
Vol 10 (1) ◽  
pp. 341 ◽  
Author(s):  
Mohamed Zaouani ◽  
Arezki Bitam ◽  
Ahcen Baz ◽  
Yasmine Benali ◽  
Meriem Hind Ben-mahdi
Keyword(s):  
Wound Healing ◽  
Methanolic Extract ◽  
Wound Contraction ◽  
Inflammatory Activity ◽  
Hydroxyproline Content ◽  
Topical Formulation ◽  
In Vivo Evaluation ◽  
Anti Inflammatory ◽  
Wound Healing Activity

ABSTRACTObjective: The present study was to evaluate the anti-inflammatory and wound healing activities of methanolic extract of Centaurea africana rootsin albino Wistar rats.Methods: Following extraction of the C. africana roots with 80% methanol, the extract was formulated as an ointment (5% and 10% w/w). The ointmentwas then evaluated for wound healing activity using excision and incision wound models. Parameters, including wound contraction, epithelizationtime, histopathologically, and hydroxyproline content, were determined using the excision model, whereas tensile strength was measured from theincision model. In parallel, edema of the ear, locally induced by croton oil, was studied for the assessment of anti-inflammatory activity.Results: Wound treated with 5% and 10% methanolic extract ointment exhibited a significant wound healing activity in both models as evidencedby increased wound contraction, shorter epithelization time, higher tissue breaking strength, and increased hydroxyproline content. The methanolicextract also produced dose-related significant reduction (p<0.001) of inflammation.Conclusion: Results of the present study revealed that C. africana displays remarkable wound healing and anti-inflammatory activities.Keywords: Anti-inflammatory activity, Centaurea africana roots, Methanolic extract, Incision and excision wound model.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

Equine limb and oral fibroblasts in in vitro models of cell proliferation and wound contraction

2009 ◽  
Vol 2009 ◽  
pp. 19-19
Author(s):  
E Watts ◽  
M T Rose
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Granulation Tissue ◽  
Collagen Matrix ◽  
In Vitro Models ◽  
Scar Formation ◽  
Wound Contraction

Wound healing in horses is particularly problematic compared to other species and limb wounds often exhibit complications such as exuberant granulation tissue, poor wound contraction and unsightly scars. In comparison, oral wounds heal without scar formation. Therefore, in vitro experiments were conducted to investigate the differences between equine oral and limb fibroblasts in terms of proliferation and their ability to contract a collagen matrix, a commonly used in vitro model of wound contraction.

scholarly journals Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

2020 ◽  
Vol 10 (5) ◽  
pp. 1845 ◽  
Author(s):  
Alexandra M. Afonso ◽  
Joana Gonçalves ◽  
Ângelo Luís ◽  
Eugenia Gallardo ◽  
Ana Paula Duarte
Keyword(s):  
Wound Healing ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Substances ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Assay ◽  
Wound Healing Activity

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.

scholarly journals Experimental Evaluation of Ethanolic Extract ofCarapa guianensisL. Leaf for Its Wound Healing Activity Using Three Wound Models

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Joel Kanhai ◽  
David Malcolm Milne ◽  
Lexley Pinto Pereira ◽  
William H. Swanston
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Breaking Strength ◽  
Test Group ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Wound Healing Activity

The leaves ofCarapa guianensishave been used to treat ulcers, skin parasites, and skin problems. The ethanolic extract ofC. guianensisleaf was evaluated for its antibacterial and wound healing activity using excision, incision and dead space wound models in rats. The animals were randomly divided into two groups (n= 6) in all the models. In the excision wound model test group animals were treated topically with the leaf extract (250 mg kg−1body weight) whereas, control animals were treated with petroleum jelly. In the incision and dead space wound models, the test group animals were treated with extract (250 mg kg−1day−1) orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight and hydoxyproline content. On Day 15 extract-treated animals exhibited 100% reduction in the wound area when compared to controls (95%) with significant decrease in the epithelialization period. The extract failed to demonstrate antibacterial activity. Skin breaking strength (P< .001), wet (P< .002) and dry (P< .02) granulation tissue and hydroxyproline content (P< .03) were significantly higher in extract treated animals. The increased rate of wound contraction, skin breaking strength and hydroxyproline content supports potential application ofC. guianensisin wound healing.

Studies in fetal wound healing VI. Second and early third trimester fetal wounds demonstrate rapid collagen deposition without scar formation

1990 ◽  
Vol 25 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Michael T. Longaker ◽  
David J. Whitby ◽  
N. Scott Adzick ◽  
Timothy M. Crombleholme ◽  
Jacob C. Langer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Scar Formation ◽  
Collagen Deposition ◽  
Third Trimester ◽  
Fetal Wound Healing ◽  
Fetal Wound

scholarly journals Wound Healing Potential of Selected Drug molecules Obtained From Terpenoids

2020 ◽  
Vol 11 (4) ◽  
pp. 7345-7352
Author(s):  
Mogan Babu N ◽  
Harikrishnan N
Keyword(s):  
Wound Healing ◽  
Significant Role ◽  
Modern Medicine ◽  
Pharmaceutical Products ◽  
High Exposure ◽  
Cytoprotective Activity ◽  
Drug Molecules ◽  
Wide Range ◽  
Anti Inflammatory ◽  
Wound Healing Activity

Skin is the vital organ of the human system; however, due to its high exposure to the external environment, it was susceptible to various traumatic conditions. During the time of trauma human body played a significant role in the activation of various biological processes for enhancing the wound healing activity in case of damaged tissues. However, in case of skin tissue defects, to enhance the angiogenesis minimal scar formation, several pharmaceutical products were paving their way into the market. Now a days there was a rising worldwide utilization of several bioactive compounds obtained from terpenoids. Terpenoids are 5-carbon compound isoprene with oxygen-containing functional groups were isolated from several plants demonstrates a wide range of activities such as antioxidant, anti-inflammatory and anti-microbial activity. Furthermore, due to their excellent cytoprotective activity, terpenoids based products had played a significant role in wound healing activity. Moreover, in our study, because of their excellent wound healing capability, we reviewed about the few drug molecules such as Tocopherol, Farnesol, Betulin, Retenoic acid, Coenzyme Q10. However, now a day's terpenoids were utilized in several wound-healing formulations because of their excellent anti-inflammatory, re-vascularization and re-epithelization characteristics. Finally, this review focuses on the role of a few drug products obtained from terpenoids as an ideal topical wound healing agent in modern medicine.

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

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