Piroxicam-Collagen-Based Sponges for Medical Applications

Depending on the concentration of the drug and/or the method of administration, drugs could be used in various ways. To take full advantage of the drug beneficial properties in oral medical interventions but also in other types of surgery, like plastic surgery, general surgery, or gynecological surgery, the drug concentration as well as the administration method itself will depend on the wound, type of surgery, and severity of the postoperative pain which can be very different. Generally, the local administration methods are recommended. Piroxicam, a nonsteroidal anti-inflammatory drug (NSAID) of the oxicam class, is generally used to relieve the symptoms of pain and inflammation. Starting from the idea of the special benefit of the interference between collagen-based materials and drug beneficial properties, our work was focused on the synthesis and characterization of new collagen-piroxicam materials. These new collagen-based materials present a good water absorption, and the piroxicam release suggests a biphasic drug release profile whereas the obtained values for the release exponent revealed a complex release mechanism including swelling, diffusion, and erosion.

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Aconitine Neurotoxicity According to Administration Methods

Journal of Clinical Medicine ◽

10.3390/jcm10102149 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2149

Author(s):

Ji Yeon Chung ◽

Seung Jae Lee ◽

Hyuck Jin Lee ◽

Jeong Bin Bong ◽

Chan-Hyuk Lee ◽

...

Keyword(s):

Nervous System ◽

Pain Control ◽

Associated Factors ◽

Clinical Characteristics ◽

Processing Method ◽

Neurological Symptoms ◽

Administration Method ◽

Accompanying Symptoms ◽

Administration Methods ◽

Human Nervous System

We evaluated the toxic effects of aconitine on the human nervous system and its associated factors, and the general clinical characteristics of patients who visited the emergency room due to aconitine intoxication between 2008 and 2017. We also analyzed the differences related to aconitine processing and administration methods (oral pill, boiled in water, and alcohol-soaked), and the clinical characteristics of consciousness deterioration and neurological symptoms. Of the 41 patients who visited the hospital due to aconitine intoxication, 23 (56.1%) were female, and most were older. Aconitine was mainly used for pain control (28 patients, 68.3%) and taken as oral pills (19 patients, 46%). The patients showed a single symptom or a combination of symptoms; neurological symptoms were the most common (21 patients). All patients who took aconitine after processing with alcohol showed neurological symptoms and a higher prevalence of consciousness deterioration. Neurological symptoms occurred most frequently in patients with aconitine intoxication. Although aconitine intoxication presents with various symptoms, its prognosis may vary with the processing method and prevalence of consciousness deterioration during the early stages. Therefore, the administration method and accompanying symptoms should be comprehensively investigated in patients who have taken aconitine to facilitate prompt and effective treatment and better prognoses.

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DESIGN AND CHARACTERIZATION OF ALFUZOSIN HCL GASTRORETENTIVE FLOATING MATRIX TABLETS EMPLOYING HPMC K 100M

INDIAN DRUGS ◽

10.53879/id.55.11.10741 ◽

2018 ◽

Vol 55 (11) ◽

pp. 71-73

Author(s):

Ch. Taraka Ramarao ◽

◽

J Vijaya Ratna ◽

R. B. Srinivasa

Keyword(s):

Drug Release ◽

Dosage Forms ◽

Matrix Tablets ◽

Fickian Diffusion ◽

Release Mechanism ◽

Gastric Residence Time ◽

Drug Release Mechanism ◽

The Matrix ◽

Gastro Retentive

The present investigation involves developing gastro retentive drug delivery systems (GFDDS) of alfuzosin HCl using HPMCK100M a is the matrixing agent and floating enhancer. Sodium bicarbonate in the acidic environment reacts with the acid and produces carbon dioxide. The gastro retentive tablets can be formulated to increase the gastric residence time and thereby increase the oral bioavailability. From the drug release study, it was concluded that the AFTB4 formula of HPMC K 100 M matrix tablets gives the controlled release up to 12 hours by showing increased release with floating lag time 24 seconds. Non – Fickian diffusion was the drug release mechanism from the matrix tablets formulated employing HPMC K 100 M. The matrix tablets (AFTB4) formulated employing 40 % HPMC K 100 M are best suited to be used for gastro retentive dosage form of alfuzosin HCl. Finally, it can be concluded that good candidates for the preparation of gastro retentive dosage forms due its gastric stability, gastric absorption and better bioavailability.

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Characterization of the nuclear export adaptor protein Nmd3 in association with the 60S ribosomal subunit

The Journal of Cell Biology ◽

10.1083/jcb.201001124 ◽

2010 ◽

Vol 189 (7) ◽

pp. 1079-1086 ◽

Cited By ~ 49

Author(s):

Jayati Sengupta ◽

Cyril Bussiere ◽

Jesper Pallesen ◽

Matthew West ◽

Arlen W. Johnson ◽

...

Keyword(s):

Binding Site ◽

Nuclear Export ◽

Large Subunit ◽

Ribosomal Subunit ◽

Adaptor Protein ◽

Structural Data ◽

Release Mechanism ◽

Nuclear Pore ◽

Subunit Joining

The nucleocytoplasmic shuttling protein Nmd3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. Nmd3 binds to nascent 60S subunits in the nucleus and recruits the export receptor Crm1 to facilitate passage through the nuclear pore complex. In this study, we present a cryoelectron microscopy (cryo-EM) reconstruction of the 60S subunit in complex with Nmd3 from Saccharomyces cerevisiae. The density corresponding to Nmd3 is directly visible in the cryo-EM map and is attached to the regions around helices 38, 69, and 95 of the 25S ribosomal RNA (rRNA), the helix 95 region being adjacent to the protein Rpl10. We identify the intersubunit side of the large subunit as the binding site for Nmd3. rRNA protection experiments corroborate the structural data. Furthermore, Nmd3 binding to 60S subunits is blocked in 80S ribosomes, which is consistent with the assigned binding site on the subunit joining face. This cryo-EM map is a first step toward a molecular understanding of the functional role and release mechanism of Nmd3.

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Formulation and Characterization of Aceclofenac-Loaded Nanofiber Based Orally Dissolving Webs

Pharmaceutics ◽

10.3390/pharmaceutics11080417 ◽

2019 ◽

Vol 11 (8) ◽

pp. 417 ◽

Cited By ~ 12

Author(s):

Emese Sipos ◽

Nóra Kósa ◽

Adrienn Kazsoki ◽

Zoltán-István Szabó ◽

Romána Zelkó

Keyword(s):

Oral Absorption ◽

Vinyl Pyrrolidone ◽

Drug Dissolution ◽

In Vitro Dissolution ◽

Drug Release Profile ◽

Scanning Calorimetry ◽

Ft Ir ◽

Fast Dissolution ◽

Poly Vinyl Pyrrolidone

Aceclofenac-loaded poly(vinyl-pyrrolidone)-based nanofiber formulations were prepared by electrospinning to obtain drug-loaded orally disintegrating webs to enhance the solubility and dissolution rate of the poorly soluble anti-inflammatory active that belongs to the BCS Class-II. Triethanolamine-containing ternary composite of aceclofenac-poly(vinyl-pyrrolidone) nanofibers were formulated to exert the synergistic effect on the drug-dissolution improvement. The composition and the electrospinning parameters were changed to select the fibrous sample of optimum fiber characteristics. To determine the morphology of the nanofibers, scanning electron microscopy was used. Fourier transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC) were applied for the solid-state characterization of the samples, while the drug release profile was followed by the in vitro dissolution test. The nanofibrous formulations had diameters in the range of few hundred nanometers. FT-IR spectra and DSC thermograms indicated the amorphization of aceclofenac, which resulted in a rapid release of the active substance. The characteristics of the selected ternary fiber composition (10 mg/g aceclofenac, 1% w/w triethanolamine, 15% w/w PVPK90) were found to be suitable for obtaining orally dissolving webs of fast dissolution and potential oral absorption.

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SYNTHESIS AND CHARACTERIZATION OF A NOVEL MUCOADHESIVE DERIVATIVE OF PSYLLIUM SEED POLYSACCHARIDE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i6.14221 ◽

2017 ◽

Vol 9 (6) ◽

pp. 166

Author(s):

Monica R.p. Rao ◽

Snehal R. Gaikwad ◽

Prachi M. Shevate

Keyword(s):

Retention Time ◽

Acute Oral Toxicity ◽

Oral Toxicity ◽

Drug Release Profile ◽

Toxicity Studies ◽

Percent Increase ◽

Human Use ◽

Ellman’S Method ◽

Thiol Functionalization

Objective: In the present study, thiol-functionalization of psyllium seed polysaccharide (PSY) was cross-linked with thioglycolic acid by esterification in an attempt to reveal the mucoadhesive properties of thiolated psyllium seed polysaccharide (TPSY).Methods: The crosslinking was carried out by the microwave-assisted method. A simplex centroid design was employed to systematically study the mucoadhesive strength, mucoadhesive retention time and drug release profile. Comparative evaluation of carbopol-based ciprofloxacin hydrochloride (HCl) tablets containing PSY and TPSY was carried out. Acute oral toxicity studies and repeated oral toxicity for TPSY were also conducted.Results: Thiol-functionalization was confirmed by-SH stretch in Fourier Transform infra-red spectra at 2353 cm-1. Thiolation was observed in thiolated PSY (TPSY) by a change in the surface morphology of psyllium from fibrous to granular and resulted in 82 %swelling in deionized water. TPSY was found to contain 102.35 mmol of thiol groups/g as determined by the Ellman’s method. The percent increase in mucoadhesive strength of TPSY was found to be 50.31 % as compared to PSY and 128.30 % as compared to carbopol. The percent increase in mucoadhesive retention time of TPSY was found to be 110 % as compared to PSY and 50 % as compared to carbopol.Conclusion: Mucoadhesion strength and mucoadhesive retention time were greater of tablets containing a higher amount of TPSY. Further, the acute oral toxicity studies and repeated oral toxicity for TPSY proved it as non-toxic and hence safe for human use.

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Characterization of PIG Platelet Granule Proteins

10.1055/s-0039-1684745 ◽

1979 ◽

Author(s):

M.H. Fukami ◽

J.L. Daniel ◽

J.S. Bauer

Keyword(s):

Gel Electrophoresis ◽

Plasma Membranes ◽

Polyacrylamide Gel Electrophoresis ◽

Dense Granule ◽

Release Mechanism ◽

Molecular Weights ◽

Dense Granules ◽

Coomassie Blue ◽

Pas Staining

Platelet granules contain glycoproteins similar to those found in platelet membranes (Hagen et al , BBA , 445, 21 4 , 1 976 ). Pig platelet granule fractions enriched in mitochondria, α-granules or dense granules were analyzed by SDS Polyacrylamide gel electrophoresis to determine if there are differences among the organelles. In a reduced system (5Ϊ OTT) the proteins of the ï-granules and dense granules showed staining patterns with Coomassie blue that were distinctly different from whole platelets, isolated membranes or mitochondria. In the granules about 10 to 12 bands with less mobility than actin were visualized. Staining with PAS was obtained in bands with apparent molecular weights of 250, 225, 185, 170, 150, 120, 55, 4B and 40 K. The 185 K band appeared to be the same as “thrombin sensitive protein”. The mobility of the 55 and 48 K hands were identical with the B (B) and γ-bands of bovine fibrinogen. The PAS staining of the granule components was more intense than that of whole platelets for the same amount of protein, indicating that granule membranes may be as rich in glycoproteins as external plasma membranes. With both PAS and Coomassie blue, the a-granule and dense granule staining patterns were almost identical. This observation may be relevant to recent studies which showed that both granule types exhibited similar release characteristics, suggesting that they share a common release mechanism. NIH-JSPHS Grant No. 14217

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Synergistic Effects of Disintegrants on Release of poorly Water soluble drug

International Journal of Pharmaceutical and Life Sciences ◽

10.3329/ijpls.v1i3.12979 ◽

2012 ◽

Vol 1 (3) ◽

Author(s):

Md Mofizur Rahman ◽

Abul Basar Khalpha ◽

Amit Kumar Chaurasiya ◽

Mithilesh Kumar Jha ◽

Md Qamrul Ahsan ◽

...

Keyword(s):

Drug Release ◽

Synergistic Effects ◽

Research Work ◽

Water Soluble ◽

Dissolution Time ◽

Release Mechanism ◽

Sodium Carboxymethylcellulose ◽

Drug Release Profile ◽

Fixed Amount ◽

Sodium Starch Glycolate

We have done research work in lab to find out proper formulation of immediate release tablet by using of various types of disintegrants (crospovidone, sodium starch glycolate and sodium carboxymethylcellulose), in order to examine the effect of mode of absorption of disintegrants on release mechanism from tablets. Acetaminophen, a poor soluble drug was used as a model drug to estimate its release pattern from different formulations. The USP paddle method was selected to perform the dissolution profiles carried out by USP apparatus 2 (paddle) at 50 rpm in 900 ml phosphate buffer pH 5.8. Successive dissolution time, time required for 25%, 50% and 80% of the drug release (T25%, T50%, T80%) was used to evaluate the dissolution results. A One way analysis of variance (ANOVA) was used to recognize the result. Statistically significant differences were found among the drug release profile from all the formulations except mode of addition of crosspovidone. At a fixed amount of disintegrants, extragranular mode of addition seemed to be the best mode of incorporation. The best release was achieved with the sodium carboxymethylcellulose and sodium starch glycolate containing formulations. The T50 and T80 values were analytical of the fact that the drug release was faster from tablet formulations containing carboxymethylcellulose and sodium starch glycolate. The drug release was very much negligible difference by the mode of crospovidone addition. Two formulations found very small T50 and T80 values indicating very much faster release. From the all formulations corresponded extragranular mode of addition could be the best mode of incorporation. The drug release was unaffected by the mode of crospovidone addition. The mode of incorporation of disintegrants suggested enchancing the release of poor soluble drugs. DOI: http://dx.doi.org/10.3329/ijpls.v1i3.12979 International Journal of Pharmaceutical and Life Sciences Vol.1(3) 2012

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Fluoxetine Dose and Administration Method Differentially Affect Hippocampal Plasticity in Adult Female Rats

Neural Plasticity ◽

10.1155/2014/123026 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 19

Author(s):

Jodi L. Pawluski ◽

Eva van Donkelaar ◽

Zipporah Abrams ◽

Virginie Houbart ◽

Marianne Fillet ◽

...

Keyword(s):

Adult Female ◽

Female Rats ◽

Granule Cell Layer ◽

Female Rat ◽

Sprague Dawley ◽

Osmotic Minipump ◽

Hippocampal Plasticity ◽

Fluoxetine Treatment ◽

Administration Method ◽

Administration Methods

Selective serotonin reuptake inhibitor medications are one of the most common treatments for mood disorders. In humans, these medications are taken orally, usually once per day. Unfortunately, administration of antidepressant medications in rodent models is often through injection, oral gavage, or minipump implant, all relatively stressful procedures. The aim of the present study was to investigate how administration of the commonly used SSRI, fluoxetine, via a wafer cookie, compares to fluoxetine administration using an osmotic minipump, with regards to serum drug levels and hippocampal plasticity. For this experiment, adult female Sprague-Dawley rats were divided over the two administration methods: (1) cookie and (2) osmotic minipump and three fluoxetine treatment doses: 0, 5, or 10 mg/kg/day. Results show that a fluoxetine dose of 5 mg/kg/day, but not 10 mg/kg/day, results in comparable serum levels of fluoxetine and its active metabolite norfluoxetine between the two administration methods. Furthermore, minipump administration of fluoxetine resulted in higher levels of cell proliferation in the granule cell layer (GCL) at a 5 mg dose compared to a 10 mg dose. Synaptophysin expression in the GCL, but not CA3, was significantly lower after fluoxetine treatment, regardless of administration method. These data suggest that the administration method and dose of fluoxetine can differentially affect hippocampal plasticity in the adult female rat.

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Effect of Administration Method on Near Point of Convergence Scores in a Healthy, Active, Young Adult Population

International Journal of Athletic Therapy & Training ◽

10.1123/ijatt.2017-0054 ◽

2018 ◽

Vol 23 (4) ◽

pp. 156-161

Author(s):

Jacqueline Phillips ◽

Kelly Cheever ◽

Jamie McKeon ◽

Ryan Tierney

Keyword(s):

Young Adults ◽

Young Adult ◽

Assessment Tool ◽

Adult Population ◽

Significant Difference ◽

Young Adult Population ◽

Administration Method ◽

Administration Methods

Near point of convergence (NPC) is an emerging concussion assessment tool and researchers have reported NPC scores using different administration methods which may influence assessment interpretation. The purpose of this study is to investigate the effect of different administrative methods on NPC scores in healthy, active young adults. NPC was measured using two different accommodative rulers and a fingertip, with three different placements. No significant difference in NPC score was observed between rulers. Significant differences were observed between ruler placements. Furthermore, fingertip use was significantly different compared to all ruler placements.

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