scholarly journals Effect of Xueniao Capsule on Escherichia coli-Induced Acute Pyelonephritis Rats by 1H NMR-Based Metabolomic Approach

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Xuliang Hao ◽  
Pan He ◽  
Yan Ni ◽  
Jing Yao ◽  
Yankun Yang ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Acute Pyelonephritis ◽  
Bacterial Count ◽  
Action Mechanism ◽  
Chemical Difference ◽  
Future Studies ◽  
H Nmr ◽  
Multiple Components ◽  
Endogenous Metabolites ◽  
Metabolomic Approach

Xueniao capsule, one of the famous traditional Chinese medicine (TCM) formulas, has been proved to be effective for treating acute pyelonephritis (APN) in the clinic. However, the probable mechanisms are still unclear. This study was aimed at investigating the therapeutic effect and action mechanism of Xueniao capsule on acute pyelonephritis rats. Chemical analysis of Xueniao capsule and four different extracts was conducted by HPLC and GC-MS. 21 compounds were identified in the Xueniao capsule, and obvious chemical difference was also revealed among the different extracts by chemical analysis. Metabolomics, combined with bacteriological examination, traditional histopathology, and biochemical parameters, was used to evaluate the effects of Xueniao capsule and four different extracts. After treatment with Xueniao capsule, the bacterial count of urine was decreased and the renal lesions of APN rats were ameliorated by histopathology inspection. Levels of Scr and Ucr, IL-1α, IL-1β, IL-6, IL-10, CXCL-2, and MCP-1 were decreased significantly, and the reserving effect of Xueniao capsule was superior to the different extracts and norfloxacin. 16 endogenous metabolites related to APN model were revealed, and 12 of them could be reversed by the Xueniao capsule. 1H NMR metabolomic results demonstrated that the formula of Xueniao capsule played the best therapeutic role on APN through regulating energy metabolism and alterations of osmotic pressure. The effect of Xueniao capsule on the APN was the synergistic actions of multiple components, which need to be further investigated in future studies.

scholarly journals 1-Norbornyl cation may be in equilibrium with 2-norbornyl cation

2019 ◽  
Vol 45 ◽  
pp. 146867831986131
Author(s):  
Andrew Mamantov
Keyword(s):  
Nmr Spectrum ◽  
Future Studies ◽  
H Nmr ◽  
C Nmr

New 1H and 13C NMR 400 MHZ spectra of the 2-Nb cation under stable ion conditions, for example, in SbF5/SO2F2/SO2ClF, −80 oC, show besides the usual 1H NMR resonances at δ 4.93, 2.82, 1.85, the never before seen singlet, δ 9.63, and doublet, δ 2.97 (J2,6 = 16.6 Hz), ratio 1.00 : 1.07, proposed to be due to resonance-stabilized bridgehead 1-Nb cationic enantiomers in equilibrium with 2-Nb cation. The corresponding 13C proton-coupled NMR spectrum, −80 oC, has a C3,5,7 triplet, δ 30.45, J(CH) = 139.14 Hz, and C4 doublet, δ 37.7, J(CH) = 154.54 Hz. The C1,2,6 absorption, δ 91.04 is relatively broad, whereas previously, at −70 oC, it was a pentuplet. The 13C proton-decoupled spectrum at −80 oC shows the C4 doublet and C3,5,7 triplet collapsed to a singlet, but the C1,2,6 resonance is still broad. Analyses support the slowing exchange between resonance stabilized enantiomeric 2-cations at ≤ –159 oC. Some future studies are proposed.

scholarly journals Characteristic Studies of Hexamethylene Diamine Complexes

2013 ◽  
Vol 2013 ◽  
pp. 1-12
Author(s):  
Hoda A. Bayoumi
Keyword(s):  
Electrical Conductivity ◽  
Schiff Base ◽  
Thermogravimetric Analysis ◽  
Chemical Analysis ◽  
Magnetic Measurements ◽  
Molar Conductance ◽  
Semiconductor Materials ◽  
Ortho Position ◽  
Hexamethylene Diamine ◽  
H Nmr

Preparation and chemical analysis of Mn(II), Fe(III), Co(II), Ni(II), and Zn(II) complexes with Schiff base L [o-HOC6H4CH:N(CH2)6N:CHC6H4OH-o] are the main tasks of this work. The octahedral (M2L2·nH2O·X) complexes in 1 : 1 M : L ratio (X = NO3− or Ac− group, L = ligand) were prepared by involving the hydroxylic group in ortho position. All complexes were characterized on the basis of elemental analysis, UV, IR, 1H NMR, Gc/Ms, thermogravimetric analysis, magnetic measurements, molar conductance, and electrical conductivity. The obtained data indicate that all the investigated compounds behave as semiconductor materials.

scholarly journals 1H NMR Metabolic Profiling of Biofluids from Rats with Gastric Mucosal Lesion and Electroacupuncture Treatment

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Jingjing Xu ◽  
Kian-Kai Cheng ◽  
Zongbao Yang ◽  
Chao Wang ◽  
Guiping Shen ◽  
...  
Keyword(s):  
Gastrointestinal Disorder ◽  
Mucosal Lesion ◽  
Proton Nuclear Magnetic Resonance ◽  
Gastric Mucosal Lesion ◽  
H Nmr ◽  
Metabolic Network Analysis ◽  
Healthy State ◽  
Stomach Meridian ◽  
And Control ◽  
Endogenous Metabolites

Gastric mucosal lesion (GML) is a common gastrointestinal disorder with multiple pathogenic mechanisms in clinical practice. In traditional Chinese medicine (TCM), electroacupuncture (EA) treatment has been proven as an effective therapy for GML, although the underlying healing mechanism is not yet clear. Here, we used proton nuclear magnetic resonance- (1H NMR-) based metabolomic method to investigate the metabolic perturbation induced by GML and the therapeutic effect of EA treatment on stomach meridian (SM) acupoints. Clear metabolic differences were observed between GML and control groups, and related metabolic pathways were discussed by means of online metabolic network analysis toolbox. By comparing the endogenous metabolites from GML and GML-SM groups, the disturbed pathways were partly recovered towards healthy state via EA treated on SM acupoints. Further comparison of the metabolic variations induced by EA stimulated on SM and the control gallbladder meridian (GM) acupoints showed a quite similar metabolite composition except for increased phenylacetylglycine, 3,4-dihydroxymandelate, and meta-hydroxyphenylacetate and decreased N-methylnicotinamide in urine from rats with EA treated on SM acupoints. The current study showed the potential application of metabolomics in providing further insight into the molecular mechanism of acupuncture.

A fingerprinting metabolomic approach reveals deregulation of endogenous metabolites after the intake of a bioactive garlic supplement

2018 ◽  
Vol 49 ◽  
pp. 137-145
Author(s):  
Álvaro Fernández-Ochoa ◽  
Isabel Borrás-Linares ◽  
Alberto Baños ◽  
J. David García-López ◽  
Enrique Guillamón ◽  
...  
Keyword(s):  
Endogenous Metabolites ◽  
Metabolomic Approach

scholarly journals 1H-NMR Metabolomics Analysis of The Intervention Effects of Ruangan Xiaoji Decoction on CCl4 Induced Liver Fibrosis in Rats

2020 ◽  
Author(s):  
Weiliang Zhu ◽  
Guoqing Wu ◽  
Wenrui Zhu ◽  
Tianwen Zhao ◽  
Hong-Jiang Zhang ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Tissue ◽  
Acid Metabolism ◽  
Clinical Chemistry ◽  
Tca Cycle ◽  
Control Group ◽  
Obvious Effect ◽  
Nmr Metabolomics ◽  
H Nmr ◽  
Endogenous Metabolites

Abstract Background: Liver fibrosis is a common consequence of chronic liver diseases resulting from multiple etiologies. Early clinical application shows that Ruangan Xiaoji Decoction (RGXJD) has a very obvious effect on the treatment of liver fibrosis. However, the mechanism of RGXJD cures liver fibrosis requires further elucidation.Methods: In this work, the therapeutic effect of RGXJD on CCl4-induced liver fibroses serum and liver tissue metabolite changes in rat was analyzed by 1H-NMR metabolomics. Meanwhile, histopathology examinations and serum clinical chemistry analysis verified the experimental results of metabolomics.Results: RGXJD treatment could reverse the increase in ALT and AST induced by CCl4 and attenuate the pathological changes in liver tissue. In the 1H-NMR metabolomic analysis, PLS-DA score plots demonstrated that the serum and liver tissue metabolic profiles in rats of the RGXJD groups were similar those of the control group, yet remarkably apart from the CCl4 group. The mechanism may be related to the endogenous metabolites including energy metabolism, amino acid metabolism, TCA cycle and purine metabolism in rats. Correlation analysis were then performed to further confirm the metabolites involved in Isoleucine, Tyrosine, UDP-Glucose, Glutathione and Leucine, etc.Conclusions: These findings may provided new insights into the mechanism of the hepatoprotection of RGXJD.

scholarly journals Infant urinary metabolomic profile in a fatal acute methadone intoxication

2022 ◽  
Author(s):  
Alberto Chighine ◽  
Michele Porcu ◽  
Giulio Ferino ◽  
Nicola Lenigno ◽  
Claudia Trignano ◽  
...  
Keyword(s):  
Perinatal Asphyxia ◽  
Sudden Infant Death ◽  
Sudden Infant ◽  
Metabolomic Profile ◽  
Metabolic Derangement ◽  
Additional Information ◽  
H Nmr ◽  
Healthy Control ◽  
Urinary Sample ◽  
Metabolomic Approach

AbstractA case report suspicious for a Sudden Infant Death Syndrome is here described. Pathological findings were consistent with an acute respiratory failure while toxicological analysis revealed an elevated blood methadone concentration. Death was then ascribed to an acute methadone intoxication. In addition to the routinary approach, the urinary sample collected at autopsy was investigated with a 1H NMR metabolomic approach and the identified metabolomic profile was challenged with the urinary metabolomic profiles previously obtained from 10 newborns who experienced perinatal asphyxia and 16 healthy control newborns. Intriguingly, the urinary profile of the methadone intoxicated infant was very similar to those belonging to the perinatal asphyxia newborns, especially to those belonging to the newborns characterised by the worst outcome. The results offer several hints on a shared metabolic derangement between different mechanisms of asphyxia/hypoxia. To the best of the authors’ knowledge, this is the first report of the use of a metabolomic approach in a pathological case, in which metabolomics offers useful additional information regarding the mechanism and the cause of death.

scholarly journals Social hierarchy and the choice of metal recycling at Anyang, the last capital of Bronze Age Shang China

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ruiliang Liu ◽  
A. Mark Pollard ◽  
Qin Cao ◽  
Cheng Liu ◽  
Victoria Sainsbury ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Bronze Age ◽  
Pure Copper ◽  
Social Hierarchy ◽  
Shang Dynasty ◽  
Future Studies ◽  
Metal Recycling ◽  
Second Millennium Bce

Abstract Anyang, the last capital of the Chinese Shang dynasty, became one of the largest metal consumers in Eurasia during the second millennium BCE. However, it remains unclear how Anyang people managed to sustain such a large supply of metal. By considering the chemical analysis of bronze objects within archaeological contexts, this paper shows that the casting and circulation of metal at Anyang was effectively governed by social hierarchy. Objects belonging to the high elites such as Fuhao, particularly the bronze ritual vessels, were made by carefully controlled alloying practice (primary) using very pure copper, whereas the lower elites only had access to bronzes made by secondary alloying practice and copper with more impurities. Such contrasts allow scholars to identify those objects which are less likely to have been made by mixing and recycling, which has very important implications for the chemical and isotopic determination of provenance for future studies.

Regulation on BCAAs catabolism pathway plays the key role in cyclophosphamide-induced leucopenia BALB/c mice after the treatment of a typical Traditional Chinese Medicine of Lvjiao Buxue Granules

2020 ◽  
Author(s):  
Jun-sheng Tian ◽  
Hui-liang Zhao ◽  
Qi Wang ◽  
Huan Xiang ◽  
Xiang-ping Xu ◽  
...  
Keyword(s):  
Cancer Chemotherapy ◽  
Blood Cells ◽  
White Blood Cells ◽  
Degradation Pathway ◽  
Integrated Analysis ◽  
Nmr Metabolomics ◽  
Biological Targets ◽  
H Nmr ◽  
Branched Chain ◽  
Endogenous Metabolites

Abstract Background: Cyclophosphamide is a common tumor chemotherapy drug used to treat various cancers, but the resulting immunosuppression leads to leukopenia, which is a serious limiting factor in clinical application. Therefore, the introduction of immunomodulators as adjuvant therapy may help to reduce the hematological side effects of cyclophosphamide. Lvjiao Buxue Granules has been widely used in clinical treatment of gynecological diseases such as anemia and irregular menstruation, and recently, it has been found to increase the role of white blood cells, but its mechanism of action is still unclear. In this research, we applied the 1H-NMR metabolomics approach to characterize metabolites in cyclophosphamide-induced leucopenia mice spleen, so as to fully understand the metabolic processes of leucopenia and improve the leukocyte function of Lvjiao Buxue Granules.Methods: Cyclophosphamide was used to establish the leucopenia mice with cancer chemotherapy and the content of white blood cells, red blood cells, hemoglobin, platelets, and other routine blood indexes were measured. The changes of endogenous metabolites in spleen analyzed by 1H-NMR metabolomics technique were investigated the regulation effect of LBG in mice with leukopenia. Afterward, the chemical components-targets-differential metabolites network of Lvjiao Buxue Granules was constructed by the use of biological targets network, thus leucopenia-relevant metabolism pathways were dissected. Results: The blood routine parameters and organ indexes levels of leucopenia mice with cancer chemotherapy were improved by Lvjiao Buxue Granules. The metabolomic study revealed that 15 endogenous metabolites in mice spleen were considered as potential biomarkers of Lvjiao Buxue Granules for its protective effect. Integrated analysis of metabolomics and biological targets network indicated that Lvjiao Buxue Granules exerted the leukocyte elevation activity by inhibiting the branched-chain amino acids (BCAAs) degradation pathway and increasing the levels of valine, leucine and isoleucine. Conclusion: Lvjiao Buxue Granules exert obvious efficacy on the mice model of leucopenia, which could be improved by regulating the branched-chain amino acid degradation pathway and the levels of valine, leucine and isoleucine.

scholarly journals Serum Metabolomic Profiling of Rats by Intervention of Aconitum soongaricum

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001
Author(s):  
Fan Zhang ◽  
Jiao Liu ◽  
Jun Lei ◽  
Wenjing He ◽  
Yun Sun
Keyword(s):  
Statistical Analysis ◽  
Nmr Spectroscopy ◽  
Metabolic Profiles ◽  
Metabolomic Analysis ◽  
Serum Samples ◽  
Multivariate Statistical ◽  
H Nmr ◽  
Metabolomic Profiling ◽  
Clinical Detection ◽  
Endogenous Metabolites

To understand the toxic mechanism and to find the changes in the endogenous metabolites of Aconitum soongaricum Stapf for clinical detection, a combination of 1H NMR spectroscopy and multivariate statistical analysis was applied to examine the metabolic profiles of the blood serum samples collected from the rat model. In total, thirteen biomarkers of A. soongaricum were found and identified. It turned out that A. soongaricum treatment may partially disorder the metabolism. The study has shown the potential application of NMR-based metabolomic analysis in providing further insights into the toxicity caused by A. soongaricum.

scholarly journals Metabonomic Study on the Antidepressant-Like Effects of Banxia Houpu Decoction and Its Action Mechanism

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Zhanqiang Ma ◽  
Weiwei Ji ◽  
Rong Qu ◽  
Mingyan Wang ◽  
Wen Yang ◽  
...  
Keyword(s):  
Experimental Model ◽  
Antidepressant Effect ◽  
Control Group ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Model Group ◽  
Action Mechanism ◽  
Significant Difference ◽  
And Control ◽  
Endogenous Metabolites

The aim of this study was to establish an experimental model for metabonomic profiles of the rat’s brain and then to investigate the antidepressant effect of Banxia Houpu decoction (BHD) and its possible mechanisms. Behavioral research and metabonomics method based on UPLC-MS were used to assess the efficacy of different fractions of BHD on chronic unpredictable mild stress (CUMS) model of depression. There was a significant difference between the BHD group and the model group. Eight endogenous metabolites, which are contributing to the separation of the model group and control group, were detected, while BHD group regulated the perturbed metabolites showing that there is a tendency of recovery compared to control group. Therefore, we think that those potential metabolite biomarkers have some relationship with BHD’s antidepression effect. This work appraised the antidepressant effect of Banxia Houpu decoction as well as revealing a metabonomics method, a valuable parameter in the TCM research.

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