scholarly journals Comparative Assessment of Affinity-Based Techniques for Oriented Antibody Immobilization towards Immunosensor Performance Optimization

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
George Tsekenis ◽  
Marianneza Chatzipetrou ◽  
Maria Massaouti ◽  
Ioanna Zergioti
Keyword(s):  
Performance Optimization ◽  
Surface Density ◽  
Critical Role ◽  
Protein A ◽  
Antigen Binding ◽  
Antibody Immobilization ◽  
Functionalized Surfaces ◽  
Antigen System ◽  
Complex Engineering ◽  
First Time

Immunosensor sensitivity and stability depend on a number of parameters such as the orientation, the surface density, and the antigen-binding efficiency of antibodies following their immobilization onto functionalized surfaces. A number of techniques have been developed to improve the performance of an immunosensor that targets one or both of the parameters mentioned above. Herein, two widely employed techniques are compared for the first time, which do not require any complex engineering of neither the antibodies nor the surfaces onto which the former get immobilized. To optimize the different surface functionalization protocols and compare their efficiency, a model antibody-antigen system was employed that resembles the complex matrices immunosensors are frequently faced with in real conditions. The obtained results reveal that protein A/G is much more efficient in increasing antibody loading onto the surfaces in comparison to boronate ester chemistry. Despite the fact, therefore, that both contribute towards the orientation-specific immobilization of antibodies and hence enhance their antigen-binding efficiency, it is the increased antibody surface density attained with the use of protein A/G that plays a critical role in achieving maximal antigen recognition.

scholarly journals Enhancing Antibodies’ Binding Capacity through Oriented Functionalization of Plasmonic Surfaces

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2620
Author(s):  
Maria Laura Coluccio ◽  
Fabiana Grillo ◽  
Valentina Onesto ◽  
Virginia Garo ◽  
Cinzia Scala ◽  
...  
Keyword(s):  
Silicon Surface ◽  
Binding Capacity ◽  
Protein A ◽  
Antigen Binding ◽  
Crucial Importance ◽  
Enhanced Fluorescence ◽  
Immobilized Antibodies ◽  
Research Fields ◽  
Fragment Antigen Binding ◽  
First Time

Protein A has long been used in different research fields due to its ability to specifically recognize immunoglobulins (Ig). The protein derived from Staphylococcus aureus binds Ig through the Fc region of the antibody, showing its strongest binding in immunoglobulin G (IgG), making it the most used protein in its purification and detection. The research presented here integrates, for the first time, protein A to a silicon surface patterned with gold nanoparticles for the oriented binding of IgG. The signal detection is conveyed through a metal enhanced fluorescence (MEF) system. Orienting immunoglobulins allows the exposition of the fragment antigen-binding (Fab) region for the binding to its antigen, substantially increasing the binding capacity per antibody immobilized. Antibodies orientation is of crucial importance in many diagnostics devices, particularly when either component is in limited quantities.

scholarly journals Apta- and Immuno-Sensors Performance Optimization: A Comparative Study of Surface Functionalization Techniques

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 847
Author(s):  
George Tsekenis ◽  
Valentina Vanikioti ◽  
Konstantinos Kordatos ◽  
Ioanna Zergioti
Keyword(s):  
Performance Optimization ◽  
Boronic Acid ◽  
Surface Coverage ◽  
Dynamic Range ◽  
Critical Role ◽  
Protein A ◽  
Wide Dynamic Range ◽  
Immobilized Antibodies ◽  
Starting Point ◽  
Modified Surfaces

Surface bio-functionalization plays a critical role in the performance of a biosensor and numerous techniques for the enhancement of a biosensor’s surface coverage with oriented capture biomolecules have been developed with the ultimate goal of optimizing a sensor’s performance in terms of its sensitivity and linear response over a wide dynamic range. Herein, highlights of a comparative assessment into the most promising approaches to achieve this goal are being presented. For aptamer-modified surfaces, polyamidoamine (PAMAM) dendrimers and polysaccharide networks were employed with the obtained results clearly indicating that a much denser surface coverage with aptamers can be achieved with the use of the latter. For the functionalization of surfaces with antibodies, the orientation and density of immobilized antibodies onto recombinant protein A/G- or boronic acid-modified substrates were compared, with the former leading not only to increased antibody loading but also with such an orientation that permits enhanced antigen binding. The conclusions reached can be used as a starting point for the customization of sensor functionalization in a plethora of clinical, environmental and even food-industry-related biosensing platforms.

Non-Plasmonic SERS with Silicon: Is It Really Safe? New Insights into Opto-Thermics of Core/Shell Microbeads

2018 ◽  
Author(s):  
Nicolò Bontempi ◽  
Irene Vassalini ◽  
Stefano Danesi ◽  
Matteo Ferroni ◽  
Paolo Colombi ◽  
...  
Keyword(s):  
Critical Role ◽  
Raman Laser ◽  
Core Shell ◽  
Thermal Coupling ◽  
Melting Points ◽  
Experimental Proof ◽  
Weakly Coupled ◽  
Light Management ◽  
Laser Sources ◽  
First Time

<p>Here we investigate for the first time the opto-thermal behavior of SiO<sub>2</sub>/Si core/shell microbeads (Si-rex) irradiated with three common Raman laser sources (lambda=532, 633, 785 nm) under real working conditions. We obtained an experimental proof of the critical role played by bead size and aggregation in heat and light management, demonstrating that in the case of strong opto-thermal coupling the temperature can exceed that of the melting points of both core and shell components. In addition, we also show that weakly coupled beads can be utilized as stable substrates for plasmon-free SERS experiments.</p>

Synthesis of an azadioxa-planar triphenylborane and investigation of its structural and photophysical properties

2021 ◽  
Author(s):  
Y. Kitamoto ◽  
K. Oda ◽  
K. Ogino ◽  
K. Hiyama ◽  
H. Kita ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Critical Role ◽  
Molecular Properties ◽  
Bridging Groups ◽  
First Time

An azadioxa-planar triphenylborane was synthesized for the first time and it was found that bridging groups have a critical role in changing its molecular properties.

scholarly journals How to evaluate sustainability of supply chains? A dynamic network DEA approach

2017 ◽  
Vol 117 (9) ◽  
pp. 1866-1889 ◽  
Author(s):  
Vahid Shokri Kahi ◽  
Saeed Yousefi ◽  
Hadi Shabanpour ◽  
Reza Farzipoor Saen
Keyword(s):  
Big Data ◽  
Supply Chains ◽  
Real World ◽  
Performance Optimization ◽  
Network Dynamic ◽  
Network Dea ◽  
Content Type ◽  
Dea Model ◽  
Proposed Model ◽  
First Time

Purpose The purpose of this paper is to develop a novel network and dynamic data envelopment analysis (DEA) model for evaluating sustainability of supply chains. In the proposed model, all links can be considered in calculation of efficiency score. Design/methodology/approach A dynamic DEA model to evaluate sustainable supply chains in which networks have series structure is proposed. Nature of free links is defined and subsequently applied in calculating relative efficiency of supply chains. An additive network DEA model is developed to evaluate sustainability of supply chains in several periods. A case study demonstrates applicability of proposed approach. Findings This paper assists managers to identify inefficient supply chains and take proper remedial actions for performance optimization. Besides, overall efficiency scores of supply chains have less fluctuation. By utilizing the proposed model and determining dual-role factors, managers can plan their supply chains properly and more accurately. Research limitations/implications In real world, managers face with big data. Therefore, we need to develop an approach to deal with big data. Practical implications The proposed model offers useful managerial implications along with means for managers to monitor and measure efficiency of their production processes. The proposed model can be applied in real world problems in which decision makers are faced with multi-stage processes such as supply chains, production systems, etc. Originality/value For the first time, the authors present additive model of network-dynamic DEA. For the first time, the authors outline the links in a way that carry-overs of networks are connected in different periods and not in different stages.

scholarly journals Prediction of IL4 Inducing Peptides

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Sandeep Kumar Dhanda ◽  
Sudheer Gupta ◽  
Pooja Vir ◽  
G. P. S. Raghava
Keyword(s):  
Mhc Class Ii ◽  
Critical Role ◽  
Interleukin 4 ◽  
Th2 Response ◽  
Data Set ◽  
T Helper 2 ◽  
Cell Responses ◽  
Antibody Class ◽  
First Time ◽  
Motif Information

The secretion of Interleukin-4 (IL4) is the characteristic of T-helper 2 responses. IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It has a critical role in guiding antibody class switching, hematopoiesis and inflammation, and the development of appropriate effector T-cell responses. In this study, it is the first time an attempt has been made to understand whether it is possible to predict IL4 inducing peptides. The data set used in this study comprises 904 experimentally validated IL4 inducing and 742 noninducing MHC class II binders. Our analysis revealed that certain types of residues are preferred at certain positions in IL4 inducing peptides. It was also observed that IL4 inducing and noninducing epitopes differ in compositional and motif pattern. Based on our analysis we developed classification models where the hybrid method of amino acid pairs and motif information performed the best with maximum accuracy of 75.76% and MCC of 0.51. These results indicate that it is possible to predict IL4 inducing peptides with reasonable precession. These models would be useful in designing the peptides that may induce desired Th2 response.

Improvement of antibody immobilization using hyperbranched polymer and protein A

2011 ◽  
Vol 409 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Guangyu Shen ◽  
Chenbo Cai ◽  
Kun Wang ◽  
Jilin Lu
Keyword(s):  
Hyperbranched Polymer ◽  
Protein A ◽  
Antibody Immobilization

Updating the knowledge of the flower flies (Diptera: Syrphidae) from Chile: Illustrated catalog, extinction risk and biological notes

Zootaxa ◽  
2021 ◽  
Vol 4959 (1) ◽  
pp. 1-178
Author(s):  
RODRIGO M. BARAHONA-SEGOVIA ◽  
PAMELA RIERA ◽  
LAURA PAÑINAO-MONSÁLVEZ ◽  
VICENTE VALDÉS GUZMÁN ◽  
PATRICIA HENRÍQUEZ-PISKULICH
Keyword(s):  
Extinction Risk ◽  
Critical Role ◽  
Floral Biology ◽  
Iucn Red List ◽  
Decomposition Of Organic Matter ◽  
Management Plans ◽  
Flower Flies ◽  
Iucn Red List Criteria ◽  
Definition Of ◽  
First Time

Syrphidae, more commonly known as flower flies, are considered one of the most important Diptera families worldwide because of their critical role in pollination, biological control and decomposition of organic matter. The study of these flies in Chile has stagnated due to a lack of local experts as well as the absence of an updated catalog of species. This study is an attempt to remedy the latter of these issues by providing an illustrated and updated catalog to the Syrphidae of Chile. Species are presented under currently accepted names, with synonyms and previous combinations listed and original references. Type localities, world and Chilean distribution by geopolitical Chilean regions, taxonomic and biological notes, a complete record of bibliographic references and extinction risk under IUCN Red List criteria are provided. This catalog recognizes 132 species of Syrphidae, belonging to four subfamilies (Eristalinae, Microdontinae, Pipizinae and Syrphinae), 13 tribes and 47 genera. A total of 46 species (34.84 %) is restricted to the geopolitical territory of Chile. Eight species are considered exotic, one is considered incertae sedis and three are based on doubtful records. Seventeen species of 10 different genera (Copestylum Macquart, 1846; Dolichogyna Macquart, 1842; Eosalpingogaster Hull, 1949b; Eupeodes Osten Sacken, 1877; Meromacrus Rondani, 1848; Palpada Macquart, 1834; Paragus Latreille, 1804; Sphiximorpha Rondani, 1850; Sterphus Philippi, 1865 and Toxomerus Macquart, 1855) are reported from Chile for the first time. A total of 44 species (33.33 %) reported from Chile are directly threatened by human activities such as agriculture, forestry, mining and/or urbanization and indirectly by climate change. The gaps found in the geographic distribution of Chilean flower fly species and what it means for its use by disciplines such as ecology, floral biology and agronomy, are discussed. In addition, the use of this illustrated catalog for biological conservation, the potential definition of priority areas and ecosystem management plans based on this group of Diptera are also discussed. 

scholarly journals IC97 Is a Novel Intermediate Chain of I1 Dynein That Interacts with Tubulin and Regulates Interdoublet Sliding

2009 ◽  
Vol 20 (13) ◽  
pp. 3044-3054 ◽  
Author(s):  
Maureen Wirschell ◽  
Chun Yang ◽  
Pinfen Yang ◽  
Laura Fox ◽  
Haru-aki Yanagisawa ◽  
...  
Keyword(s):  
Kinase Inhibitors ◽  
Critical Role ◽  
Protein A ◽  
Suppressor Gene ◽  
Lung Tumorigenesis ◽  
Candidate Tumor Suppressor Gene ◽  
Dynein Motor ◽  
Biochemical Assays ◽  
Regulatory Complex ◽  
And Control

Our goal is to understand the assembly and regulation of flagellar dyneins, particularly the Chlamydomonas inner arm dynein called I1 dynein. Here, we focus on the uncharacterized I1-dynein IC IC97. The IC97 gene encodes a novel IC without notable structural domains. IC97 shares homology with the murine lung adenoma susceptibility 1 (Las1) protein—a candidate tumor suppressor gene implicated in lung tumorigenesis. Multiple, independent biochemical assays determined that IC97 interacts with both α- and β-tubulin subunits within the axoneme. I1-dynein assembly mutants suggest that IC97 interacts with both the IC138 and IC140 subunits within the I1-dynein motor complex and that IC97 is part of a regulatory complex that contains IC138. Microtubule sliding assays, using axonemes containing I1 dynein but devoid of IC97, show reduced microtubule sliding velocities that are not rescued by kinase inhibitors, revealing a critical role for IC97 in I1-dynein function and control of dynein-driven motility.

scholarly journals Obesity Accelerates Age-Associated Defects in Human B Cells Through a Metabolic Reprogramming Induced by the Fatty Acid Palmitate

2022 ◽  
Vol 2 ◽  
Author(s):  
Daniela Frasca ◽  
Maria Romero ◽  
Denisse Garcia ◽  
Alain Diaz ◽  
Bonnie B. Blomberg
Keyword(s):  
Fatty Acid ◽  
B Cells ◽  
Critical Role ◽  
Metabolic Reprogramming ◽  
Autoimmune Antibodies ◽  
Human B Cells ◽  
Initial Hypothesis ◽  
First Time ◽  
Culture Supernatants

We have measured the secretion of autoimmune antibodies in plasma samples and in culture supernatants of blood-derived B cells from four groups of individuals: young lean (YL), elderly lean (EL), young obese (YO) and elderly obese (EO). We found secretion comparable in YO and EL individuals, suggesting that obesity accelerates age-associated defects in circulating B cells. To define at least one possible molecular pathway involved, we used an in vitro model in which B cells from YL and EL individuals have been stimulated with the Fatty Acid (FA) palmitate, the most common saturated FA in the human body. The rationale to use palmitate is that there is a chronic increase in circulating levels of palmitate, due to increased spontaneous lipolysis occurring during aging and obesity, and this may induce autoimmune B cells. Results herein show that in vitro incubation of B cells from YL and EL individuals with the FA palmitate induces mRNA expression of T-bet, the transcription factor for autoimmune antibodies, as well as secretion of autoimmune IgG antibodies, with B cells from YL individuals looking similar to B cells from EL individuals, confirming our initial hypothesis. The generation of autoimmune B cells in the presence of the FA palmitate was found to be associated with a metabolic reprogramming of B cells from both YL and EL individuals. These results altogether show the critical role of the FA palmitate in inducing human B cell immunosenescence and show for the first time the importance of metabolic pathways in this process.

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