scholarly journals Antioxidant, Antibacterial, and Anticancer Activities of Bitter Gourd Fruit Extracts at Three Different Cultivation Stages

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Syed Ali Raza Naqvi ◽  
Shafaqat Ali ◽  
Tauqir A. Sherazi ◽  
Atta-Ul Haq ◽  
Muhammad Saeed ◽  
...  
Keyword(s):  
Antibacterial Activities ◽  
Bitter Gourd ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Anticancer Activities ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Ripening Stages ◽  
Dried Fruit ◽  
Pure Methanol

In this study, we are presenting the effect of three ripening stages of air-dried bitter gourd fruit extracts on phenolic acid composition, antioxidant, antibacterial, and anticancer activities. The results showed mature bitter gourd fruit extract in 100% methanol showing 78% DPPHº scavenging activity. Immature dried fruit extract in 80% and 100% methanol showed promising antibacterial activities, i.e., >18.5 ± 0.21 mm zone-of-inhibition against Staphylococcus aureus, while mature dried fruit extract in 80% methanol showed 18.4 ± 0.17 mm zone-of-inhibition against Escherichia coli. Anticancer activity results of 100% methanol extracts of ripened fruit possess showed 6.72 ± 1.81 and 3.55 ± 0.51 mg/mL IC50 values with HeLa and MDBK cancer cell lines, respectively. The overall results indicate that the immature and ripen fruits of BG could be extracted in pure methanol as an antibacterial and anticancer phytomedicine.

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals Evaluation of antifungal activity of Terminalia subspathulata bark and fruit extracts against azole-resistant Candida isolates

2021 ◽  
Vol 3 (12) ◽  
Author(s):  
Hassan Yahaya ◽  
Sharif Alhassan Abdullahi ◽  
Yahaya Yakubu
Keyword(s):  
Antifungal Susceptibility ◽  
Diffusion Method ◽  
Bark Extract ◽  
Zone Of Inhibition ◽  
Fruit Extract ◽  
Alternative Source ◽  
Disk Diffusion Method ◽  
Severe Problem ◽  
Fruit Extracts ◽  
Methanolic Extracts

Background: Emerging resistant Candida species become a severe problem for public health. The study aims to examine the efficacy of Terminalia subspathulata bark and fruit extracts as an alternative source of antifungals for resistant strains of Candida species. Methodology: Candida albicans was isolated between 4–5th interdigital foot space and C. parapsilosis were isolated from the left-hand third finger of male patients. Morphological and molecular methods identified the isolates. Antifungal susceptibility testing (AFST) was determined using azole derivatives, amphotericin B (AmpB) and echinocandins based on E-test and broth microdilution (BMD) methods. The AFST activity of the 10 μL methanolic extracts with concentrations between 1.25–10 mg/mL was assessed by disk diffusion method according to CLSI guidelines. Results: The AFST results showed total resistance to all azoles (FLU, MIC ≥ 256 μg/ml and VOR, ITR and POS each with MIC ≥ 32 μg/ml). The BMD method agreed with E-test MICs. The T. subspathulata bark extract tested indicated activity on the C. albicans lawn with the zone of inhibition between 9–13 mm. The fruit extract indicated zone between 8–10 mm. Candida parapsilosis indicated susceptibility to bark extract with the zone of inhibition between 10–15 mm, while the fruit extract showed zone between 8–13 mm. Conclusion: Despite azole resistance, the extracts showed good activity against the isolates. Findings showed alternatives to AmpB and echinocandins because of toxicity and parenteral administration, respectively. Based on our findings, our study first reports the medicinal value of T. subspathulata.

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test. The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals Investigation of phytochemical composition, evaluation of antioxidant, antibacterial activities and toxicity study of Emblica officinalis and Terminalia bellirica fruits

2016 ◽  
Vol 9 (6) ◽  
pp. 96 ◽  
Author(s):  
Himani Badoni ◽  
Saumya Singh ◽  
Promila Sharma ◽  
Syed Mohsin Waheed
Keyword(s):  
Antibacterial Activities ◽  
Toxicity Study ◽  
Total Phenolic ◽  
Emblica Officinalis ◽  
Bacterial Strains ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Maximum Inhibition ◽  
Total Flavonoids Content ◽  
Terminalia Bellirica

The present study deals with exploration of various beneficial potential of Emblica officinalis and Terminalia bellirica fruit extracts which includes phytochemical analysis and evaluation of total phenolics content (TPC), total flavonoids content (TFC) and antimicrobial activity. The antioxidant activity of the extracts was examined using diphenylpicrylhydrazyl (DPPH), TPC and TFC assay. The antimicrobial activity of the extracts was evaluated by agar well diffusion assay. Although, the extracts prevented the growth of both Gram positive and Gram negative bacteria. The EOA and EOM extracts both exhibit tannins, saponins, phenols and carbohydrates whereas, alkaloids and flavonoids are found only in EOM, and glycosides showed their presence in aqueous extract only. In contrast, TBA and TBM extracts, both contains alkaloids, tannins, coumarins, flavonoids and phenols. While carbohydrates are present barely in TBM, and TBA extract showed the presence of saponins and glycosides. The TPC results show that EOM (528±.013) has highest phenolic content in it followed by EOA (509±.003), TBM (284±.06) and TBA (280±.036). In TFC analysis too EOM (154±0) showed a higher concentration range of flavonoids followed by EOA (142±0),TBM (126±.017)  and TBA (119±.007). In DPPH scavenging assay EOM (86.88±.310) showed maximum % inhibition followed by EOA (85.32±.414), TBM (80.57±.569) and TBA (75.55±.362) respectively. Antibacterial assay showed varying results for different bacterial strains such as, for EOA (10±1)  demonstrated  highest inhibition against E. coli, EOM (11.5±0.5) exhibited maximum inhibition against S. aureus, for K. pneumoniae EOA (13±2) displayed maximum inhibition and in case P. aeruginosa too EOA (12.5±0.5) showed maximum inhibition while TBM and TBA too showed significant amount of inhibition against test bacterial strains. The antibacterial and antioxidant activities of the extracts were found to be positively associated with the total phenolic and flavonoid content of the extracts. This study was conducted to identify the phytochemical composition of the fruit extracts of the two plants, their antioxidant and antibacterial potential, alongwith their neuroprotective and nootropic role. In order to establish their neuroprotective role, acute toxicity study was conducted on wistar rats to establish the safety of the extracts. The current study demonstrates and compares the antioxidant, antibacterial activities of the fruit extracts of Terminalia bellirica and Emblica officinalis and acute toxicity study further demonstrates that the extracts used are safe to conduct neuroprotective studies. Keywords: TPC(Total phenolic content), TFC(Total flavonoids content), EOM (Emblica officinalis Methanolic fruit extract), EOA(Emblica officinalis aqueous fruit extract) TBM (Terminalia bellirica Methanolic fruit extract) and TBA (Terminalia bellirica aqueous fruit extract).

scholarly journals MORTALITAS, PENGHAMBATAN MAKAN DAN PERTUMBUHAN HAMA DAUN GAHARU HEORTIA VITESSOIDES MOORE OLEH EKSTRAK BUAH BRUCEA JAVANICA (L.) MERR.

2012 ◽  
Vol 12 (2) ◽  
pp. 119-128
Author(s):  
Agus M. Hariri
Keyword(s):  
Growth Inhibition ◽  
Leaf Area ◽  
Emerging Adult ◽  
The Other ◽  
Fruit Extract ◽  
Dried Fruits ◽  
Residual Method ◽  
Fruit Extracts ◽  
Brucea Javanica ◽  
Dried Fruit

The bioassay of  Brucea javanica fruit extract was performed to evaluate lethal and antifeedance effect as well as growth inhibition against agarwood defoliator Heortia vitessoides.  Three types of Brucea (unripe, ripe, and dried fruits) were extracted with maceration method in aquadest for 24 hours. Tests were done using leaf-residual method.  The results showed that mortality of H. vitessoides larvae was significantly higher when applied with Brucea extract at  concentration 50 g/l  compared to control starting at two days after application. Moreover the dried fruit extract killed all  insect test in three days after application. The other result indicated that pupation of agarwood defoliator was less than 10% and emerging adult  was 0% when applied with Brucea fruit extracts.  In addition, at two days after application of ripe and dried fruit extracts resulted antifeedance effect against H. vitessoides larvae 68-70% leaves weight and 74-77% based on eaten leaf area.

scholarly journals Antioxidant Capacity and Phenolic and Sugar Profiles of Date Fruits Extracts from Six Different Algerian Cultivars as Influenced by Ripening Stages and Extraction Systems

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 503
Author(s):  
Malika Tassoult ◽  
Djamel Edine Kati ◽  
María África Fernández-Prior ◽  
Alejandra Bermúdez-Oria ◽  
Juan Fernandez-Bolanos ◽  
...  
Keyword(s):  
Sugar Content ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Antioxidant Power ◽  
Fruit Extracts ◽  
Ripening Stages ◽  
Date Fruit ◽  
Ferric Reducing Antioxidant Power ◽  
First Time

The study investigated the phenols, sugar and the antioxidant capacities of date fruit extracts obtained by organic solvents and by hydrothermal treatment from six different Algerian cultivars at two ripening stages for the first time. The analyzed cultivars exhibited potent antioxidant properties (ferric reducing antioxidant power (FRAP), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacities) and different phenols regardless of the solvents and the maturity stages. About 18 phenols were identified and quantified, mainly in the hydrothermal extracts. The earlier stages were characterized by high amounts of o-coumaric acid, cinnamic acid and luteolin, with a noticeable absence of quercetin. The tamr stage presented the highest sugar content (78.15–86.85 mg/100 mg dry weight (DW)) with an abundance of glucose. Galactose was present only in some cultivars from the kimri stage (tamjouhert). Uronic acids were mostly detected at the tamr stage (4.02–8.82 mg gallic acid equivalent/100 mg dried weight). The obtained results highlight the potential of using date fruit extracts as natural antioxidants, especially at industrial scales that tend use hydrothermal extraction.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

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