Immunomodulatory and Antioxidant Activities of Select Indonesian Vegetables, Herbs, and Spices on Human Lymphocytes

Edible plants have attracted increasing attention as functional foods as they are rich in bioactive compounds with health benefits, including antioxidant and immunomodulatory activities. However, scientific evidence of these health effects is limited. This study is aimed at determining antioxidant and immunomodulatory activities of 25 select vegetables, herbs, and spices commonly consumed in Indonesia. Phytochemical profiles were determined by measuring total flavonoid content and 1H-NMR. Human blood lymphocyte cells were used to probe the immunomodulatory potency and treated with the methanol extract of these vegetables, herbs, and spices. The results showed the enhanced propensity for all tested plant extracts to stimulate lymphocyte proliferation, except Pandanus amaryllifolius. Etlingera elatior, Ocimum xcitriodorum, Kaempferia galanga, and Apium graveolens had the highest lymphocyte cell proliferation stimulation index (SI) at concentrations of 41.67, 16.67, 4.17, and 2.5 mg/mL culture, respectively (SI 2.21 ± 0.05 , 2.62 ± 0.12 , 3 ± 0.05 , and 2.64 ± 0.07 , respectively). The NMR spectra of these four most potent plants showed low peaks in the aromatic/phenolic area and several other peaks indicating the presence of terpenoid, steroid, amino acid, and sugar compounds. The results demonstrate the immunomodulatory potential of all vegetables, herbs, and spices, except P. amaryllifolius, although this potential did not necessarily correlate with flavonoid content and antioxidant activity. Nevertheless, this research showed promising health effect, particularly immunomodulation, of the various local plants. Further elaboration on the specific immunomodulatory activity will be interesting.

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Survey of Chinese Cowpea Cultivars for Phenolic Compounds and Antioxidant Activity

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.17:337-348 ◽

2018 ◽

Vol 17 (4) ◽

pp. 337-348

Author(s):

Bai Zhouya ◽

Huang Xiaojun ◽

Meng Jinxia ◽

Kan Lijiao ◽

Nie Shaoping

Keyword(s):

Phenolic Compounds ◽

Phenolic Content ◽

Antioxidant Activities ◽

Jiangxi Province ◽

Total Phenolic ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Comprehensive Investigation ◽

Cowpea Cultivars ◽

P Distribution

Distribution of phenolic compounds and antioxidant activities was surveyed in 24 varieties of Chinese cowpea from 4 provinces. Identity of phytochemicals were determined by UPLC-ESIQTOF-MS/MS and quantified by HPLC-ESI-QqQ-MS/MS. Seven phenolic acids, 16 flavonoids and 9 other compounds were identified and several of these were quantified. Quercetin-3-glucoside and kaempferol-glucoside were the major phenolic compounds. In addition, our study indicated that OJYDH cultivar from Jiangxi province had the highest total phenolic content, total flavonoid content and stronger antioxidant activities than other cowpea cultivars. This study made a comprehensive investigation on antioxidants from cowpea and provided the useful data to support its function.

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Establishing Relationship between Vitamins, Total Phenolic and Total Flavonoid Content and Antioxidant Activities in Various Honey Types

Molecules ◽

10.3390/molecules26154399 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4399

Author(s):

Norhasnida Zawawi ◽

Pei Juin Chong ◽

Nurul Nadhirah Mohd Tom ◽

Nurkhairina Solehah Saiful Anuar ◽

Salma Malihah Mohammad ◽

...

Keyword(s):

Antioxidant Capacity ◽

Phenolic Acids ◽

Antioxidant Activities ◽

Natural Antioxidants ◽

Total Phenolic ◽

Flavonoid Content ◽

Extrinsic Factors ◽

Biochemical Compounds ◽

Phenolic And Flavonoid

Honey is a well-known natural sweetener and is rich in natural antioxidants that prevent the occurrence of oxidative stress, which is responsible for many human diseases. Some of the biochemical compounds in honey that contribute to this property are vitamins and phenolic compounds such as phenolic acids and flavonoids. However, the extent to which these molecules contribute towards the antioxidant capacity in vitro is inconsistently reported, especially with the different analytical methods used, as well as other extrinsic factors that influence these molecules’ availability. Therefore, by reviewing recently published works correlating the vitamin, total phenolic, and flavonoid content in honey with its antioxidant activities in vitro, this paper will establish a relationship between these parameters. Based on the literature, vitamins do not contribute to honey’s antioxidant capacity; however, the content of phenolic acids and flavonoids has an impact on honey’s antioxidant activity.

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Comparison of the antioxidant activities of 22 commonly used culinary herbs and spices on the lipid oxidation of pork meat

Animal Science Journal ◽

10.1046/j.1344-3941.2002.00054.x ◽

2002 ◽

Vol 73 (5) ◽

pp. 389-393 ◽

Cited By ~ 56

Author(s):

Hisako TANABE ◽

Masami YOSHIDA ◽

Nanae TOMITA

Keyword(s):

Lipid Oxidation ◽

Antioxidant Activities ◽

Pork Meat ◽

Culinary Herbs ◽

Herbs And Spices

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Guidelines for the Intake of Vegetables and Fruit: the Mediterranean Approach

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.71.3.149 ◽

2001 ◽

Vol 71 (3) ◽

pp. 149-153 ◽

Cited By ~ 14

Author(s):

Antonia Trichopoulou ◽

Androniki Naska ◽

Effie Vasilopoulou

Keyword(s):

Beneficial Effect ◽

Red Wine ◽

Vegetable Intake ◽

Vegetable Consumption ◽

Scientific Evidence ◽

Daily Intake ◽

Black Tea ◽

Dietary Guidelines ◽

Food Chemistry ◽

Flavonoid Content

Various studies have demonstrated that the nutrient and non-nutrient substances present in vegetables and fruit (V&F) are most likely to be responsible for the beneficial effect of the increased V&F consumption. Urged by scientific evidence, current dietary guidelines strongly recommend the consumption of V&F in substantial amounts. In a recent paper (Brit. J. Nutr. 2000; 84, 549-556) V&F availability in 10 European countries was compared with the WHO recommendations (minimum combined V&F intake of about 400 g/day/person), as well as with guidelines of a minimum daily intake of three portions of vegetables (approx. 250 g/person) and two portions of fruit (approx. 150 g/person). All countries, excluding Greece, had a vegetable intake below the recommended minimum. Moreover, in all countries, the percentages of low vegetable consumers were significantly higher than those of low fruit consumers, suggesting that there is considerable room for improvement in the intake of vegetables, an important source of antioxidants. Wild edible greens are among the vegetables commonly consumed in Greece. These greens have a high flavonoid content, which in several cases substantially exceeds the respective values in foods and beverages, such as onions, black tea and red wine (Food Chemistry 2000; 70, 319-323). The high flavonoid content of edible wild greens requires consideration of their role in contemporary diet, as a possible mean for increasing vegetable consumption.

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ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF DIFFERENT PARTS OF SUPER RED DRAGON FRUIT (HYLOCEREUS COSTARICENSIS) COLLECTED FROM WEST JAVA-INDONESIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21571 ◽

2017 ◽

Vol 10 (12) ◽

pp. 290 ◽

Cited By ~ 2

Author(s):

Irda Fidrianny ◽

Nadia Ilham ◽

Rika Hartati

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Total Phenolic ◽

Flavonoid Content ◽

Phytochemical Content ◽

Dragon Fruit ◽

Dpph Method ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

Different Parts

Objectives: The goals of this research were to observe antioxidant properties from different parts of super red dragon fruit (Hylocereus costaricensis) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).Methods: Antioxidant activities were determined using DPPH and ABTS assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method.Results: Inhibitory concentration 50% (IC50) of DPPH scavenging activity of all of the extracts in the range of 2.69 μg/ml was −94.17 μg/ml. The ethyl acetate peel extract of super red dragon fruit expressed the highest TPC (4.56 g GAE/100 g) and the highest total flavonoid content (12.63 g QE/100 g). TPC in flesh extract of super red dragon fruit had a negative and significant correlation with their IC50 of ABTS. The IC50 of DPPH and IC50 of ABTS of flesh extract of super red dragon fruit showed positive and significant correlation.Conclusion: All different parts extracts of super red dragon fruit (except n-hexane flesh extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in flesh extract of super red dragon fruit were the major contributor in antioxidant activities by ABTS method. DPPH and ABTS showed linear results in antioxidant activities of super red dragon fruit flesh extract.

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Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from Lippia alba Essential Oils in an Experimental Model of Chronic Chagas Disease

Antioxidants ◽

10.3390/antiox10111851 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1851

Author(s):

Denerieth Ximena Espinel-Mesa ◽

Clara Isabel González Rugeles ◽

Julio César Mantilla Hernández ◽

Elena E. Stashenko ◽

Carlos Andrés Villegas-Lanau ◽

...

Keyword(s):

Essential Oils ◽

Chagas Disease ◽

Antioxidant Activities ◽

Combined Therapy ◽

Late Phase ◽

Immunomodulatory Activity ◽

Caryophyllene Oxide ◽

Cardiac Damage ◽

Lippia Alba ◽

Chronic Chagas Disease

In the late phase of Trypanosoma cruzi infection, parasite persistence and an exaggerated immune response accompanied by oxidative stress play a crucial role in the genesis of Chronic Chagasic Cardiomyopathy (CCC). Current treatments (Benznidazole (BNZ) and Nifurtimox) can effect only the elimination of the parasite, but are ineffective for late stage treatment and for preventing heart damage and disease progression. In vivo trypanocidal and cardioprotective activity has been reported for Lippia alba essential oils (EOs), ascribed to their two major terpenes, limonene and caryophyllene oxide. To investigate the role of antioxidant and immunomodulatory mechanisms behind these properties, chronic-T. cruzi-infected rats were treated with oral synergistic mixtures of the aforementioned EOs. For this purpose, the EOs were optimized through limonene-enrichment fractioning and by the addition of exogenous caryophyllene oxide (LIMOX) and used alone or in combined therapy with subtherapeutic doses of BNZ (LIMOXBNZ). Clinical, toxicity, inflammatory, oxidative, and parasitological (qPCR) parameters were assessed in cardiac tissue. These therapies demonstrated meaningful antioxidant and immunomodulatory activity on markers involved in CCC pathogenesis (IFN-γ, TNF-α, IL-4, IL-10, and iNOS), which could explain their significant trypanocidal properties and their noteworthy role in preventing, and even reversing, the progression of cardiac damage in chronic Chagas disease.

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Total Phenolic and Flavonoid Content and Antioxidant Activities of Ten Malaysian Wild Mushrooms

OALib ◽

10.4236/oalib.1103987 ◽

2017 ◽

Vol 04 (11) ◽

pp. 1-9

Author(s):

Jinting Azieana ◽

Mohd Noor Zainon ◽

Abdullah Noriham ◽

Mat Nor Rohana

Keyword(s):

Antioxidant Activities ◽

Total Phenolic ◽

Flavonoid Content ◽

Wild Mushrooms ◽

Phenolic And Flavonoid

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Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

International Research Journal of Pure and Applied Chemistry ◽

10.9734/irjpac/2020/v21i430160 ◽

2020 ◽

pp. 1-5

Author(s):

M. Suleman Stephen ◽

E. A. Adelakun ◽

J. H. Kanus ◽

Meshack M. Gideon

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Acetone Extract ◽

Apium Graveolens ◽

Isolation And Identification ◽

Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

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