scholarly journals Comparing the Antifungal Effect of Sodium Hypochlorite Gel versus Different Types of Root Canal Medicaments at Different Time Intervals Using the Agar Diffusion Test: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Mohamed El Sayed ◽  
Nikta Ghanerad ◽  
Zeinab Shabanpour ◽  
Mahin Shabanpoor ◽  
Fatemeh Rahimi
Keyword(s):  
Antifungal Activity ◽  
Calcium Hydroxide ◽  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Significant Inhibition ◽  
Time Interval ◽  
Time Intervals ◽  
Significance Level ◽  
Agar Diffusion Test

Aims. The purpose of this study was to compare the antifungal activity of sodium hypochlorite gel to those of four intracanal medicaments at various time intervals. Materials and Methods. The agar well diffusion technique was utilized to test the antifungal activity of the following medicaments against Candida albinans (C. albicans): sodium hypochlorite gel, chlorhexidine gel, calcium hydroxide paste, Ledermix, and Diapex Plus. The inhibition zone related to each medicine was measured in millimeter after 24, 48, and 72 hours of incubation at 37°C. The data were analyzed using one-way ANOVA and Games–Howell tests, at a significance level of P < 0.05 . Results. Except for Ledermix and Diapex Plus, which had no antifungal action, all of the medicaments showed varied inhibitory zones for C. albicans. At all periods, the NaOCl gel had the most significant inhibition zones, followed by the CHX gel and calcium hydroxide. At all time intervals, the NaOCl gel demonstrated comparable antifungal efficacy. When compared to a 24-hour time interval, the CHX gel showed an increased antifungal activity at the 48-hour and 72-hour intervals. Calcium hydroxide, on the other hand, showed a decrease in its antifungal activity at the 72-hour interval. Conclusion. The antifungal activity of sodium hypochlorite gel was the highest among the investigated medicaments. Chlorhexidine gel's antifungal activity improved over time, whereas calcium hydroxide's antifungal activity declined. Diapex Plus and Ledermix did not have any antifungal properties.

Effectiveness of calcium hydroxide plus points and chlorhexidine activ points against Enterococcus faecalis by agar diffusion test: An in-vitro study

2013 ◽  
Vol 1 (1) ◽  
pp. 18 ◽  
Author(s):  
Venkatesh Alagarsamy ◽  
Senthil Kumar ◽  
AjitGeorge Mohan ◽  
AV Rajesh Ebenezar ◽  
MR Srinivasan
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Diffusion Test ◽  
Agar Diffusion ◽  
Agar Diffusion Test

scholarly journals Effect of Incorporation of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone: An in vitro Study

2017 ◽  
Vol 7 (3) ◽  
pp. 86-91
Author(s):  
Anubhuti Bakshi ◽  
Rajat Dang
Keyword(s):  
Sodium Hypochlorite ◽  
Testing Machine ◽  
Surface Hardness ◽  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Control Group ◽  
Time Interval ◽  
Type Iv ◽  
Post Hoc

ABSTRACT Aim To evaluate surface hardness and dimensional accuracy of type IV dental stone after incorporation of disinfectants. Materials and methods A total of 100 specimens, 50 (40 mm⨯ 10 mm) disk shaped and 50 frustum cone shaped, were fab- ricated from type IV dental stone after incorporation of various disinfectants, namely glutaraldehyde (2%), iodophor (1.76%), chlorhexidine (2%), and sodium hypochlorite (1%), for evaluation of surface hardness and dimensional accuracy. Surface hardness was tested after 1- and 24-hour time interval using Rockwell hard- ness testing machine. Dimensional accuracy was measured using traveling microscope. Data obtained were compiled and analyzed statistically by using analysis of variance and post hoc test. Results Results showed significant decrease in surface hard- ness of samples incorporated with disinfectants at 1 hour as compared with control (distilled water). Surface hardness of all the samples increased with time and was almost equal to control group at 24-hour interval, with iodophor showing the maximum value in comparison with the other disinfectants used. When dimensional accuracy of all the groups was compared, iodophor and sodium hypochlorite were found to cause negative alterations. Conclusion Within the limitations of this in vitro study, it was concluded that incorporation of disinfectants in die stone during mixing did not affect the hardness value at 24-hour interval. Dimensional accuracy was altered by iodophor and sodium hypochlorite, but remained unaffected by chlorhexidine and glutaraldehyde. How to cite this article Bakshi A, Dang R. Effect of Incorpo- ration of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone An in vitro Study. Int J Prosthodont Restor Dent 2017;7(3):86-91.

Evaluation of topical therapies for the treatment of dermatophyte-infected hairs from dogs and cats

1995 ◽  
Vol 31 (3) ◽  
pp. 250-253 ◽  
Author(s):  
N White-Weithers ◽  
L Medleau
Keyword(s):  
Antifungal Activity ◽  
Sodium Hypochlorite ◽  
Povidone Iodine ◽  
Fungal Growth ◽  
In Vitro Study ◽  
Test Period ◽  
Microsporum Canis ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution

Seven commonly used, topical antifungal products (i.e., lime sulfur, chlorhexidine, captan, povidone-iodine, sodium hypochlorite, and enilconazole solutions, and ketoconazole shampoo) were evaluated for their antifungal activity on Microsporum canis-infected hairs from dogs and cats in an in vitro study. Hairs were soaked or shampooed in each product for five minutes twice a week for four weeks. Of the seven products used in this study, lime sulfur and enilconazole solutions were superior in inhibiting fungal growth; no growth occurred on fungal cultures after two treatments with either product. Chlorhexidine and povidone iodine solutions were effective after four treatments, and sodium hypochlorite solution and ketoconazole shampoo inhibited fungal growth after eight treatments. Captan did not inhibit fungal growth during the test period.

scholarly journals An in vitro Study to evaluate the Effects of Various Polymerizing Conditions on the Flexural Strength of Autopolymerizing Resins

2017 ◽  
Vol 8 (1) ◽  
pp. 15-19
Author(s):  
Sanjeev Singh, ◽  
Abid Z Hussain, ◽  
RU Thombare, ◽  
BK Motwani, ◽  
Shweta Singh,
Keyword(s):  
Flexural Strength ◽  
Constant Pressure ◽  
Testing Machine ◽  
In Vitro Study ◽  
Bending Test ◽  
Control Group ◽  
Time Interval ◽  
Time Intervals ◽  
Increased Strength

ABSTRACT Aim To investigate the effect of variation in temperature and time intervals on the flexural strength of two brands of autopolymerizing resins polymerized at constant pressure. Materials and methods Two autopolymerizing resins RR Cold Cure resin (DPI) and RR-Rapid Repair Powder (Dentsply) were used. A total of 20 samples were fabricated for each group. Samples were polymerized at temperature ranges of 40°, 50°, 60°, and 70°C and at time intervals of 5, 10, and 15 minutes in the customized curing pot, maintaining pressure of 30 psi. Results Flexural strength was verified using universal testing machine using three-point bending test and was then compared with all samples and also with the samples cured in open air (control group). The samples cured at higher temperature, i.e., 60° and 70°C, have shown increased strength than those at 40° and 50°C as well as control group. Time interval of 10 minutes is considered sufficient to increase the strength of the resin, as per the results of this study. Conclusion The samples cured under varied condition of time and temperature but under constant pressure showed increased strength than those cured in open air. Clinical significance The effect of pressure and temperature has been demonstrated, which could be utilized for enhancing the durability of the prosthesis fabricated from autopolymerized resins. How to cite this article Singh S, Hussain AZ, Thombare RU, Motwani BK, Singh S, Mangalekar SB. An in vitro Study to evaluate the Effects of Various Polymerizing Conditions on the Flexural Strength of Autopolymerizing Resins. J Health Sci Res 2017;8(1):15-19.

scholarly journals Antibacterial effectiveness in vitro of different formulations of calcium hydroxide paste

2017 ◽  
Vol 65 (4) ◽  
pp. 293-298
Author(s):  
Israel Alexandre De Araujo SENA ◽  
Isaac Jordão De Souza ARAÚJO ◽  
Marquiony Marques Dos SANTOS ◽  
Isabela Pinheiro Cavalcanti LIMA
Keyword(s):  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Significant Inhibition ◽  
Diffusion Method ◽  
Significance Level ◽  
Root Canals ◽  
Infected Root ◽  
Inhibition Zones

ABSTRACT Objective: To evaluate the antibacterial activity of four formulations of calcium hydroxide paste against microorganisms commonly found in infected root canals. Methods: To evaluate antibacterial activity through the agar diffusion method, pastes of calcium hydroxide were made from its pro-analysis form, diffused into four separate vehicles: distilled water, camphorated p-monochlorophenol, propylene glycol and Otosporin®, testing the antimicrobial activity of these on strains of Staphylococcus aureus, Bacillus subtilis and Enterococcus faecalis. After the incubation period, the presence or otherwise of inhibition zones were observed and their sizes in three stages: 24h, 48h and 72h. With this data, the median between the four dishes was obtained and the consequent value was submitted to Kruskal-Wallis nonparametric statistical analysis, with post-tests of Mann-Whitney and Bonferroni correction, at a significance level of 5%. Results: Only pastes with camphorated p-monochlorophenol and Otosporin vehicles caused the formation of significant inhibition zones, with medians of 8.0 mm. Against the strains of Enterococcus faecalis, only pastes with the camphorated p-monochlorophenol vehicle resulted in the formation of significant inhibition zones, with a median of 3.0 mm. Conclusion : Otosporin and CMCP vehicles provide greater antimicrobial potential to calcium hydroxide against the studied bacteria. However, only the Ca(OH)2 and CMCP combination was effective against all the strains, and can thus be regarded as the paste formulation with the greatest antimicrobial effectiveness in this study.

scholarly journals Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test

2003 ◽  
Vol 14 (2) ◽  
pp. 99-102 ◽  
Author(s):  
Luciana Moura Sassone ◽  
Rivail Antonio Sergio Fidel ◽  
Sandra Rivera Fidel ◽  
Marina Dias ◽  
Raphael Hirata Junior
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Fusobacterium Nucleatum ◽  
Time Interval ◽  
Time Intervals ◽  
Contact Test

The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.

scholarly journals Remineralization of carious dentin. I: In vitro microradiographic study in human teeth capped with calcium hydroxide

2004 ◽  
Vol 15 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Carlos Alberto Conrado
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Content ◽  
In Vitro Study ◽  
Bottom Layer ◽  
Time Intervals ◽  
Human Teeth ◽  
Carious Teeth ◽  
Demineralized Dentin ◽  
Control Samples

The objective of this in vitro study was to evaluate a possible remineralization of human carious dentin by calcium hydroxide. Thirty-nine freshly extracted human permanent and deciduous carious teeth were split into two halves. One half was used as control and the other as experimental. In the latter, a cavity was prepared and the remaining bottom layer of demineralized dentin capped with chemically pure calcium hydroxide. The experimental samples were stored at 37ºC. Time intervals were 2, 4, 6, 8, 10 and 12 weeks. All tooth halves were sawed and ground to plano-parallel sections with a thickness varying from 75 to 117 µm. Qualitative microradiographs showed a qualitative increase in radiopacity of the calcium hydroxide treated samples. Quantitative microradiography showed a statistically significant increase in total mineral content in the experimental samples compared to the control samples. These results indicate an in vitro remineralization of carious dentin by calcium hydroxide.

scholarly journals Effect of addition of lycopene to calcium hydroxide and chlorhexidine as intracanal medicament on fracture resistance of radicular dentin at two different time intervals: An in vitro study

2015 ◽  
Vol 18 (3) ◽  
pp. 205
Author(s):  
Koppolu Madhusudhana ◽  
Chinni Suneelkumar ◽  
Kasamsetty Archanagupta ◽  
Anumula Lavanya ◽  
Mandava Deepthi
Keyword(s):  
Calcium Hydroxide ◽  
Fracture Resistance ◽  
In Vitro Study ◽  
Vitro Study ◽  
Time Intervals

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

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