Metabolomic Study of Zuojin Pill in Relieving 1-Methyl-3-nitro-1-nitrosoguanidine-Induced Chronic Atrophic Gastritis

The classic prescription Zuojin Pill (ZJP) shows a good therapeutic effect on chronic atrophic gastritis (CAG); it is of great significance to clarify its specific mechanism. Therefore, we explore the mechanism of ZJP on MNNG-induced CAG by integrating approaches. First of all, through the pathological changes of gastric tissue and the expression level of PGI and PGI/II in serum, the expression of inflammation-related factors was determined by RT-PCR to determine the efficacy. Then, UPLC-Q-TOF/MS was used for plasma and urine metabolomic analysis to screen the specific potential biomarkers and metabolic pathway of ZJP in ameliorating CAG and to explore its possible mechanism. ZJP significantly ameliorate the pathological injury of gastric tissue, increase levels of PGI and PGI/II, and reduce the expression level of proinflammatory factors. Through metabolomic analysis, 9 potential metabolic differences were identified and 6 related metabolic pathways were enriched. These findings indicate for the first time the potential mechanism of ZJP in improving CAG induced by MNNG and are of great significance to the clinical development and application of ZJP-related drugs.

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Acupuncture Decreases NF-κB p65, miR-155, and miR-21 and Increases miR-146a Expression in Chronic Atrophic Gastritis Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/9404629 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Jialing Zhang ◽

Kangbai Huang ◽

Guoxin Zhong ◽

Yong Huang ◽

Suhe Li ◽

...

Keyword(s):

Atrophic Gastritis ◽

Nuclear Factor Kappa B ◽

Acupuncture Treatment ◽

Chronic Atrophic Gastritis ◽

Nuclear Factor ◽

Gastric Tissue ◽

Model Group ◽

Weight Changes ◽

Gastric Glands ◽

Histological Changes

Acupuncture has been used to treat chronic atrophic gastritis (CAG) in traditional Chinese medicine (TCM) for centuries. In this study, we evaluated the effect of acupuncture at Zusanli (ST36), Zhongwan (CV12), and Pishu (BL20) acupoints on weight changes of rats, histological changes of gastric glands, and expressions changes of nuclear factor-kappa B (NF-κB) p65, microRNA- (miR-) 155, miR-21, and miR-146a in CAG rats induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) combined with irregular diet. Consequently, we found that acupuncture treatment elevated body weight of rats significantly when compared to the model group. By observing histological changes, we found that the acupuncture group showed better improvement of gastric mucosa injury than the model group. Our results also demonstrated upregulation of NF-κB p65, miR-155, and miR-21 in gastric tissue of CAG rats and a positive correlation between miR-155 and miR-21. Relatively, expression of miR-146a was downregulated and negative correlation relationships between miR-146a and miR-155/miR-21 in CAG rats were observed. Additionally, expressions of NF-κB p65, miR-155, and miR-21 were downregulated and miR-146a was upregulated after acupuncture treatment. Taken together, our data imply that acupuncture can downregulate NF-κB p65, miR-155, and miR-21 and upregulate miR-146a expression in CAG rats. NF-κB p65, miR-155, miR-21, and miR-146a may play important roles in therapeutic effect of acupuncture in treating CAG.

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The expression of Cx43, TGFβ/smads signaling pathways and PCNA in the occurrence and development of gastric carcinoma and the relationship among them

Asian Journal of Medical Sciences ◽

10.3126/ajms.v7i1.12593 ◽

2015 ◽

Vol 7 (1) ◽

pp. 6-13

Author(s):

Chun-Hui Li ◽

Li-Hui Pan ◽

Hai-Wang Liu ◽

Rajina Sahi ◽

Xing Zhao ◽

...

Keyword(s):

Gastric Carcinoma ◽

Expression Level ◽

Gastric Tissue ◽

Rt Pcr ◽

Age And Gender ◽

Expression Levels ◽

Node Metastasis ◽

Immuno Histochemistry ◽

And Gender ◽

The Relationship

Aims and Objectives: To detect the protein expression level of Cx43, smad4 and PCNA in normal and gastric carcinoma tissue by immuno-histochemistry, western blotting and RT-PCR method. We intend to reveal the relationship between their expression in occurrence and development. Simultaneously through analyzing experimental data we have obtained the correlation among them.Materials and Methods: Immuno-histochemistry and western blotting were adopted to detect the expression of Cx43, smad4 and PCNA protein in normal and carcinomatous gastric tissue, expression levels mRNA of Cx43, smad4 and PCNA were detected by RT-PCR in normal and carcinomatous gastric tissue. Experimental data were analyzed by combining clinical pathology data using statistical approach. Results: The expression levels of Cx43, and smad4 in carcinomatous gastric tissue were lower than they were in normal gastric tissue. The expression level of PCNA in carcinomatous gastric tissue was higher than that in normal gastric tissue. The expression levels of Cx43, Smad4 and PCNA in groups of age and gender is not significant. The expression of Cx43, Smad4 and PCNA in different clinicopathologic features of carcinomatous gastric tissue including degrees of pathological differentiation, lymph node metastasis condition, depth of tumor invasion and TNM stages is statistically significant. With the progression of gastric carcinoma, the expression levels of Cx43 and Smad4 were decreased, conversely PCNA showed a high expression level. Based on the analysis Cx43 negatively correlated with PCNA, Cx43 positively correlated with Smad4, Smad4 negatively correlated with PCNA.Conclusions: The protein and mRNA expressions of Cx43, smad4 and PCNA were significant differences between normal gastric tissue and gastric carcinoma, also were concerned with differentiation degree, lymph node metastasis, TNM stages and depth of invasion, while not significant with age and gender. This suggests that they may play an important role in occurrence and development of gastric carcinoma, but also may have some interaction. Asian Journal of Medical Sciences Vol.7(1) 2015 6-13

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Protective Effect of Zuojin Pill on Helicobacter Pylori-Induced chronic atrophic gastritis in Rats and GES-1 Cells and Mechanisms of Action Exploration

10.21203/rs.3.rs-20652/v1 ◽

2020 ◽

Author(s):

Shihua Wu ◽

Chunmei Bao ◽

Ruilin Wang ◽

Jianzhong Zhang ◽

Juling Zhang ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gastric Mucosa ◽

Protein Expression ◽

Cell Viability ◽

Atrophic Gastritis ◽

Therapeutic Effect ◽

Chronic Atrophic Gastritis ◽

Expression Level ◽

Cox 2 ◽

H Pylori

Abstract Objective Zuojin Pill (ZJP) containing two Chinese herbal drugs: Coptidis Rhizoma and Euodiae Fructus is a classical formula and is widely accepted as a treatment of chronic atrophic gastritis (CAG) in China. This study aimed to explore the therapeutic effect and mechanism of ZJP which attenuated H. pylori -induced CAG in vivo and in vitro.Methods: H. pylori (Helicobacter pylori) was used to induce CAG rat model. 0.63, 1.26, and 2.52 g/kg of ZJP (was administered orally for four weeks. Therapeutic effect of ZJP was identified by H & E staining and serum indices. In addition, cell viability, morphology and proliferation were detected by cell counting kit-8 and high-content screening assay. Gene and protein expression related to JMJD2B/COX-2/VEGF axis were detected to further investigate the potential mechanism.Results Compared with the control group, the ZJP groups showed a significant protection effects on Gastric mucosa, as indicated by the reduced loss of glands and inflammatory cell infiltration. Meanwhile, ZJP could ameliorate cell viability, morphology changes, and proliferation in GES-1 cells. Moreover, the ZJP treatment decreased the amount of IL-8, and TNF-α, indicating that it could reduce the level of inflammation, and decrease stomach damage. The expression of JMJD2B/COX-2/VEGF axis related genes and proteins were measured by real-time quantitative PCR, western blot and immunohistochemistry methods. The ZJP groups were found to decrease relative genes and protein expression level compared with the model group. ZJP could improve gastric mucosa protection and reduce inflammation level by inhibiting the expression level of JMJD2B/COX-2/VEGF axis.Conclusion Our data confirmed the effective therapy of ZJP in H. pylori -induced CAG, which supports the role of ZJP as an anti-inflammatory and protection of gastric mucosa agent in CAG induced by H. pylori . These results may provide helpful tools for the treatment of CAG.

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Chronic Atrophic Gastritis: A Review

Journal of Environmental Pathology Toxicology and Oncology ◽

10.1615/jenvironpatholtoxicoloncol.2018026839 ◽

2018 ◽

Vol 37 (3) ◽

pp. 241-259 ◽

Cited By ~ 10

Author(s):

Yifei Li ◽

Ran Xia ◽

Bo Zhang ◽

Chunsheng Li

Keyword(s):

Atrophic Gastritis ◽

Chronic Atrophic Gastritis

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Effects of weifuchun tablet for chronic atrophic gastritis

Medicine ◽

10.1097/md.0000000000020374 ◽

2020 ◽

Vol 99 (22) ◽

pp. e20374

Author(s):

Liangjun Yang ◽

Zhipeng Hu ◽

Jiajie Zhu ◽

Baoying Fei

Keyword(s):

Atrophic Gastritis ◽

Chronic Atrophic Gastritis

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Phylogenomic Evidence of Reinfection and Persistence of SARS-CoV-2: First Report from Colombia

Vaccines ◽

10.3390/vaccines9030282 ◽

2021 ◽

Vol 9 (3) ◽

pp. 282

Author(s):

Juan David Ramírez ◽

Marina Muñoz ◽

Nathalia Ballesteros ◽

Luz H. Patiño ◽

Sergio Castañeda ◽

...

Keyword(s):

Viral Persistence ◽

Transmission Dynamics ◽

Polymorphism Analysis ◽

Rt Pcr ◽

Paired Samples ◽

Infection Dynamics ◽

Oxford Nanopore ◽

Novel Variants ◽

First Time ◽

Oxford Nanopore Technologies

The continuing evolution of SARS-CoV-2 and the emergence of novel variants have raised concerns about possible reinfection events and potential changes in the coronavirus disease 2019 (COVID-19) transmission dynamics. Utilizing Oxford Nanopore technologies, we sequenced paired samples of three patients with positive RT-PCR results in a 1–2-month window period, and subsequent phylogenetics and genetic polymorphism analysis of these genomes was performed. Herein, we report, for the first time, genomic evidence of one case of reinfection in Colombia, exhibiting different SARS-CoV-2 lineage classifications between samples (B.1 and B.1.1.269). Furthermore, we report two cases of possible viral persistence, highlighting the importance of deepening our understanding on the evolutionary intra-host traits of this virus throughout different timeframes of disease progression. These results emphasize the relevance of genomic surveillance as a tool for understanding SARS-CoV-2 infection dynamics, and how this may translate effectively to future control and mitigations efforts, such as the national vaccination program.

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Epidemiology of chronic atrophic gastritis: population-based study among 9444 older adults from Germany

Alimentary Pharmacology & Therapeutics ◽

10.1111/j.1365-2036.2007.03430.x ◽

2007 ◽

Vol 26 (6) ◽

pp. 879-887 ◽

Cited By ~ 44

Author(s):

M. N. WECK ◽

C. STEGMAIER ◽

D. ROTHENBACHER ◽

H. BRENNER

Keyword(s):

Older Adults ◽

Atrophic Gastritis ◽

Chronic Atrophic Gastritis ◽

Population Based ◽

Population Based Study

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Molecular epidemiology of Crimean-Congo haemorrhagic fever virus in India

Epidemiology and Infection ◽

10.1017/s0950268816001886 ◽

2016 ◽

Vol 144 (16) ◽

pp. 3422-3425 ◽

Cited By ~ 1

Author(s):

P. SINGH ◽

M. CHHABRA ◽

P. SHARMA ◽

R. JAISWAL ◽

G. SINGH ◽

...

Keyword(s):

Eastern Europe ◽

N Gene ◽

Western India ◽

Haemorrhagic Fever ◽

Rt Pcr ◽

Study Region ◽

Phylogenetic Relatedness ◽

Cchf Virus ◽

Crimean Congo Haemorrhagic Fever ◽

First Time

SUMMARYCrimean-Congo haemorrhagic fever (CCHF) is an emerging zoonotic disease in India which is prevalent in neighbouring countries. CCHF virus (CCHFV) is a widespread tick-borne virus which is endemic in Africa, Asia, Eastern Europe and the Middle East. In the present study, samples of clinically suspected human cases from different areas of northern-western India were tested for the presence of CCHFV by RT–PCR through amplification of nucleocapsid (N) gene of CCHFV. Positive samples were sequenced to reveal the prevailing CCHFV genotype(s) and phylogenetic relatedness. A phylogenetic tree revealed the emergence of diverse strains in the study region showing maximum identity with the Pakistan, Afghanistan and Iran strains, which was different from earlier reported Indian strains. Our findings reveal for the first time the emergence of the Asia 1 group in India; while earlier reported CCHFV strains belong to the Asia 2 group.

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Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Pathogens ◽

10.3390/pathogens10060674 ◽

2021 ◽

Vol 10 (6) ◽

pp. 674

Author(s):

Anton Yuzhakov ◽

Ksenia Yuzhakova ◽

Nadezhda Kulikova ◽

Lidia Kisteneva ◽

Stanislav Cherepushkin ◽

...

Keyword(s):

Infectious Disease ◽

Genetic Diversity ◽

Acute Gastroenteritis ◽

Rt Pcr ◽

Clinical Hospital ◽

Group A Rotavirus ◽

Group A ◽

First Time ◽

Common Combination ◽

Children Under 5

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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