scholarly journals Permeability Characteristics of Water-Sand Seepage in Fracture by Experiment

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Yu Liu ◽  
Shuncai Li ◽  
Wei Li ◽  
Zhihao Luo ◽  
Liming Wu ◽  
...  
Keyword(s):  
Particle Size ◽  
Water Inrush ◽  
Great Influence ◽  
Side Wall ◽  
Hysteresis Curve ◽  
Sand Particle ◽  
Type I ◽  
Permeability Characteristics ◽  
Type Iv ◽  
Seepage Characteristics

Studies on the seepage characteristics of water-sand cracks are of great significance to reveal the mechanisms of water and sand inrush. Using a self-made water-sand fracture seepage test instrument, a water-sand seepage test was carried out, and the permeability of water and sand in the fracture was determined. The hysteresis characteristics of water-sand flow in the fracture were obtained after the required permeability was attained. The results show that the hysteresis curve changes from type I to type IV with the increase in sand particle size and concentration. The hysteresis parameters are described by the maximum hysteresis Gp ∗ and the hysteresis area S, both of which show an increasing trend with the increase in sand particle size and concentration; however, this increase is not synchronous. The average velocity and turbulent kinetic energy distribution of the water-sand fluid on the fracture cross section are greatly affected by the particle size and concentration of the volume of sand. This study can provide a reference for further study of water inrush from a shallow coal seam. Through simulation, it is found that the particle size has a great influence on the seepage velocity, and the influence near the side wall surface is greater than that in the middle position.

scholarly journals Experimental Study of the Water-Sediment Two-Phase Seepage Characteristics in Rock Fractures and the Influencing Factors

Geofluids ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Kui Di ◽  
Ming Li ◽  
Xianbiao Mao ◽  
Zhanqing Chen ◽  
Lianying Zhang ◽  
...  
Keyword(s):  
Particle Size ◽  
Pressure Gradient ◽  
Volume Fraction ◽  
Water Inrush ◽  
Experimental System ◽  
Sand Particle ◽  
Two Phase ◽  
Absolute Value ◽  
The Absolute ◽  
Seepage Characteristics

The water-sediment two-phase seepage in coarse fractures is one of the major factors to trigger mine water inrush disasters. Based on seepage mechanics theory, a mechanical model of the water-sediment two-phase seepage in coarse fractures was established. An experimental system was also developed to study the seepage characteristics under various conditions. The relationships between the absolute value of the pressure gradient and the seepage velocity were analyzed during the test process. The nonlinear characteristics of the seepage test were revealed. In addition, variation laws of the absolute value of the pressure gradient with the sand volume fraction and the sand particle size were illustrated, which were related to the loss of pressure during the particle movement. The impacts of the sand volume fraction and the sand particle size on the equivalent fluidity and β -factor of non-Darcy flow were discussed and analyzed. It was determined that the local turbulence was the main reason for the change of nonlinear variation characteristics of seepage parameters.

scholarly journals Experimental Study of Hysteresis Characteristics of Water-Sediment Mixture Seepage in Rock Fractures

Geofluids ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Lili Cao ◽  
Pu Zhang ◽  
Jiazhi Zhang ◽  
Gang Lin ◽  
Izhar Mithal Jiskani ◽  
...  
Keyword(s):  
Particle Size ◽  
Coal Mine ◽  
Mass Concentration ◽  
Water Inrush ◽  
Sand Particle ◽  
Rock Fractures ◽  
Fracture Opening ◽  
Sediment Mixture ◽  
And Control ◽  
Hysteresis Characteristics

The hysteresis of water-sediment mixture seepage in rock fractures is one of the critical factors which affect the determination of the timing of coal mine water inrush disasters prevention and control. In this paper, a mechanical model was established to study the hysteresis whose criteria were also put forward. The area of the hysteresis loop and the maximum pressure gradient were selected as characterization parameters of hysteresis. On this basis, an experimental system was established to study influences of different sand particle size, sand mass concentration, and fracture opening on water-sediment mixture seepage in rock fractures. The results indicated that the increase in the sand particle size and sand mass concentration could effectively enhance hysteresis characteristics of specimen fractures. While hysteresis characteristics decreased significantly with the increase of fracture opening. The research results are useful to prevent and control water inrush disasters of coal mine.

scholarly journals Study on Fracture and Permeability Characteristics of Unloading Rock with High Water Pressure

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Zhi-tao Ma ◽  
Yong-qiang Cui ◽  
Ke-yu Lu ◽  
Dai-fu Song ◽  
Ya-chao Yang
Keyword(s):  
Permeability Coefficient ◽  
Failure Modes ◽  
Water Pressure ◽  
Water Inrush ◽  
High Stress ◽  
High Water ◽  
Permeability Characteristics ◽  
Finite Element Software ◽  
Seepage Characteristics ◽  
High Water Pressure

Research on the damage and seepage characteristics of unloading rock with high water pressure can help to further understand the mechanism of water inrush in deep mine floor and prevent water inrush. This paper used the RFPA2D-flow finite element software to study the failure and seepage characteristics of unloading rock with high water pressure and high stress and comparatively analyzed the failure modes and seepage characteristics of unloading rock with and without water pressure. The effects of different water pressure differences on the failure of unloading rock and the law of seepage were investigated by analyzing the change of acoustic emission and permeability coefficient with stress. The results showed that the unloading rock without water pressure was brittle failure, and the initial damage of the unloading model with water pressure was earlier than that of the model without water pressure and showed greater brittleness, and its cracks first break through at the bottom of the sample with higher osmotic pressure. With the increase in unloading, the permeability of rock increased gradually until it appeared an abrupt change. The failure mode and permeability law of the rock with different water pressure differences were basically the same, but the greater the pressure difference, the smaller the effective unloading capacity when the permeability coefficient changes suddenly, and the greater the possibility of water inrush in the rock.

Labelling of non-A, non-B hepatitis infected chimpanzee hepatocytes with nuclease-gold conjugates

1984 ◽  
Vol 42 ◽  
pp. 250-251
Author(s):  
G. D. Gagne ◽  
M. F. Miller ◽  
D. A. Peterson
Keyword(s):  
Endoplasmic Reticulum ◽  
Experimental Infection ◽  
Uranyl Acetate ◽  
Type I ◽  
Cellular Injury ◽  
Type Ii ◽  
Tubular Structures ◽  
Type Iii ◽  
Type Iv ◽  
Infected Chimpanzee

Experimental infection of chimpanzees with non-A, non-B hepatitis (NANB) or with delta agent hepatitis results in the appearance of characteristic cytoplasmic alterations in the hepatocytes. These alterations include spongelike inclusions (Type I), attached convoluted membranes (Type II), tubular structures (Type III), and microtubular aggregates (Type IV) (Fig. 1). Type I, II and III structures are, by association, believed to be derived from endoplasmic reticulum and may be morphogenetically related. Type IV structures are generally observed free in the cytoplasm but sometimes in the vicinity of type III structures. It is not known whether these structures are somehow involved in the replication and/or assembly of the putative NANB virus or whether they are simply nonspecific responses to cellular injury. When treated with uranyl acetate, type I, II and III structures stain intensely as if they might contain nucleic acids. If these structures do correspond to intermediates in the replication of a virus, one might expect them to contain DNA or RNA and the present study was undertaken to explore this possibility.

scholarly journals Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 14
Author(s):  
Thamiris Santana Machado ◽  
Felipe Ramos Pinheiro ◽  
Lialyz Soares Pereira Andre ◽  
Renata Freire Alves Pereira ◽  
Reginaldo Fernandes Correa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Protein A ◽  
Invasive Infection ◽  
Type I ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Spa Gene ◽  
The City

Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.

scholarly journals Distribution of virulence genes and SCCmec types among methicillin-resistant Staphylococcus aureus of clinical and environmental origin: a study from community of Assam, India

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Deepshikha Bhowmik ◽  
Shiela Chetri ◽  
Bhaskar Jyoti Das ◽  
Debadatta Dhar Chanda ◽  
Amitabha Bhattacharjee
Keyword(s):  
Staphylococcus Aureus ◽  
Virulence Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Virulence Gene ◽  
Type I ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Clinical Community ◽  
Type V ◽  
Sccmec Types

Abstract Objective This study was designed to discover the dissemination of virulence genes in Methicillin-resistant Staphylococcus aureus from clinical, community and environmental settings. Results This study includes 1165 isolates collected from hospital, community and environmental settings. Among them sixty three were confirmed as MRSA with varied SCCmec types viz; type I, type II, type III, type IV, type V, type VI, type VII, type VIII and type XII. The virulence gene such as sea (n = 54), seb (n = 21), eta (n = 27), etb (n = 2), cna (n = 24), ica (n = 2) and tst (n = 30) was also revealed from this study. The study underscores coexistence of resistance cassette and virulence genes among clinical and environment isolates which is first of its kind from this part of the world.

scholarly journals Brachial supporting structure of Spiriferida (Brachiopoda)

2020 ◽  
pp. 1-15
Author(s):  
Zhiwei Yuan ◽  
Wen Guo ◽  
Dan Lyu ◽  
Yuanlin Sun
Keyword(s):  
Mantle Cavity ◽  
Family Type ◽  
Type I ◽  
Type Ii ◽  
Feeding Apparatus ◽  
Single Family ◽  
Supporting Structure ◽  
Type Iii ◽  
Type Iv ◽  
Evolutionary Lineages

Abstract The filter-feeding organ of some extinct brachiopods is supported by a skeletal apparatus called the brachidium. Although relatively well studied in Atrypida and Athyridida, the brachidial morphology is usually neglected in Spiriferida. To investigate the variations of brachidial morphology in Spiriferida, 65 species belonging to eight superfamilies were analyzed. Based on the presence/absence of the jugal processes and normal/modified primary lamellae of the spiralia, four types of brachidium are recognized. Type-I (with jugal processes) and Type-II (without jugal processes), both having normal primary lamellae, could give rise to each other by losing/re-evolving the jugal processes. Type-III, without jugal processes, originated from Type-II through evolution of the modified lateral-convex primary lamellae, and it subsequently gave rise to Type-IV by evolving the modified medial-convex primary lamellae. The evolution of brachidia within individual evolutionary lineages must be clarified because two or more types can be present within a single family. Type-III and Type-IV are closely associated with the prolongation of the crura, representing innovative modifications of the feeding apparatus in response to possible shift in the position of the mouth towards the anterior, allowing for more efficient feeding on particles entering the mantle cavity from the anterior gape. Meanwhile, the modified primary lamellae adjusted/regulated the feeding currents. The absence of spires in some taxa with Type-IV brachidium might suggest that they developed a similar lophophore to that in some extant brachiopods, which can extend out of the shell.

scholarly journals Patterns and Relevance of Langerhans Islet Invasion in Pancreatic Cancer

Cancers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 249
Author(s):  
Ruediger Goess ◽  
Ayse Ceren Mutgan ◽  
Umut Çalışan ◽  
Yusuf Ceyhun Erdoğan ◽  
Lei Ren ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Islet Cells ◽  
Human Pancreatic Cancer ◽  
Type I ◽  
Type Iv ◽  
Pancreatic Cancer Cells

Background: Pancreatic cancer‐associated diabetes mellitus (PC‐DM) is present in most patients with pancreatic cancer, but its pathogenesis remains poorly understood. Therefore, we aimed to characterize tumor infiltration in Langerhans islets in pancreatic cancer and determine its clinical relevance. Methods: Langerhans islet invasion was systematically analyzed in 68 patientswith pancreatic ductal adenocarcinoma (PDAC) using histopathological examination and 3D in vitro migration assays were performed to assess chemoattraction of pancreatic cancer cells to isletcells. Results: Langerhans islet invasion was present in all patients. We found four different patterns of islet invasion: (Type I) peri‐insular invasion with tumor cells directly touching the boundary, but not penetrating the islet; (Type II) endo‐insular invasion with tumor cells inside the round islet; (Type III) distorted islet structure with complete loss of the round islet morphology; and (Type IV)adjacent cancer and islet cells with solitary islet cells encountered adjacent to cancer cells. Pancreatic cancer cells did not exhibit any chemoattraction to islet cells in 3D assays in vitro. Further, there was no clinical correlation of islet invasion using the novel Islet Invasion Severity Score (IISS), which includes all invasion patterns with the occurrence of diabetes mellitus. However, Type IV islet invasion was related to worsened overall survival in our cohort. Conclusions: We systematically analyzed, for the first time, islet invasion in human pancreatic cancer. Four different main patterns of islet invasion were identified. Diabetes mellitus was not related to islet invasion. However, moreresearch on this prevailing feature of pancreatic cancer is needed to better understand underlying principles.

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