scholarly journals Spectroscopic Investigation of Chlorin-Based Photosensitizers in Polymer Matrix

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Antonina Naumenko ◽  
Nataliya Kutsevol
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Optical Absorption ◽  
Tumor Cells ◽  
Polymer Matrix ◽  
Spectroscopic Investigation ◽  
Chlorin E6 ◽  
Optical Absorption Spectra ◽  
Trimethyl Ether ◽  
Derivatives Of

Chlorin e6 and its derivatives are the basis of a number of drugs used in medicine in the treatment of various diseases, including cancer, by photodynamic therapy. Nonpolar derivatives of Chlorin e6—dimethyl ether of Chlorin e6 (DME Ce6) and trimethyl ether of Chlorin e6 (TME Ce6)—are actively studied for application during photodynamic therapy. In this work, based on the electron optical absorption spectra, the interaction of photosensitizer molecules with branched star-like copolymer dextran-graft-polyacrylamide in anionic form was investigated and the possibility of using the latter as a carrier for drug delivery to tumor cells was suggested.

Optical Properties of Photodynamic Therapy Drugs in Different Environments: The Paradigmatic Case of Temoporfin

2020 ◽  
Author(s):  
busenur Aslanoglu ◽  
Ilya Yakavets ◽  
Vladimir Zorin ◽  
Henri-Pierre Lassalle ◽  
Francesca Ingrosso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Optical Properties ◽  
Photodynamic Therapy ◽  
Minimally Invasive ◽  
Visible Light ◽  
Cancer Treatment ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Molecular Oxygen ◽  
Computational Tools

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.

scholarly journals Photosensitizer Chlorin e6 Internalization into Tumor Cells in Phospholipid Nanoparticles Conjugated with Peptide Containing the NGR Sequence

2018 ◽  
Vol 1 (4) ◽  
pp. e00063 ◽  
Author(s):  
V.N. Prozorovskiy ◽  
L.V. Kostryukova ◽  
E.I. Korotkevich ◽  
T.I. Torkhovskaya ◽  
G.E. Morozevich ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Amino Acid ◽  
Tumor Cells ◽  
Targeted Delivery ◽  
Aminopeptidase N ◽  
Chlorin E6 ◽  
Phospholipid Nanoparticles ◽  
Chlorine E6 ◽  
Targeting Peptide ◽  
Mcf 7

The possibility of increased internalization of the photosensitizer chlorin e6 in tumor cells was investigatedusing soy phosphatidylcholine nanoparticles 20-30 nm with or without attached peptide containing Asn-Gly-Arg (NGR) motif was studied. This amino acid sequence exhibits affinity to aminopeptidase N (CD13), wich is overexpressed in a number of tumor cells and vessels. Nanoparticles with chlorin e6 were prepared with added of distearoylphosphatidylcholine (DSPE) conjugated through PEG with a hexapeptide containing the NGR sequence, and then were incubated with tumor cells НерG2 and MCF-7. Chlorin e6 accumulation in СD13-negative cells (MCF-7) did not depend on the presence of peptide NGR in nanoparticles. However, for НерG2 cells a twofold increase of chlorine e6 internalization was observed as compared with the same particles without NGR. Differences in the response of these two cell lines, differed in expression of aminopeptidase N (APN), suggest the possibility of this protein using for targeted delivery. The prospectivity of usage of phospholipids nanoparticles conjugated with targeting peptide for photodynamic therapy is discussed, taking into account possible variation of APN expression, inherent for many solid tumors.

scholarly journals Programmable Ce6 Delivery via Cyclopamine Based Tumor Microenvironment Modulating Nano-System for Enhanced Photodynamic Therapy in Breast Cancer

2019 ◽  
Vol 7 ◽  
Author(s):  
Chan Feng ◽  
Lv Chen ◽  
Yonglin Lu ◽  
Jie Liu ◽  
Shujing Liang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Tumor Microenvironment ◽  
Cancer Cell Line ◽  
Chlorin E6 ◽  
Great Promise ◽  
Tissue Destruction ◽  
Mice Model ◽  
Cell Internalization

Photodynamic therapy (PDT) has shown great promise in breast cancer treatment. However, simplex target ligand modification or stimuli release cannot meet the requirement of effective drug delivery to solid tumor tissue. To overcome continuous bio-barriers existing in the tumor microenvironment, multi-stage response drug delivery was desirable. Herein, we developed a unique tumor microenvironment tailored nanoplatform for chlorin e6 (Ce6) delivery. We chose bovine serum albumin (BSA) as “mother ships” material for effective tumor periphery resident, cyclopamine (CYC) as extracellular matrix (ECM) inhibitor and synergistic anti-tumor agent, and diselenide containing amphiphilic hyaluronic acid-chlorin e6 polymers (HA-SeSe-Ce6) synthesized as “small bombs” for internal tissue destruction. The above three distinct function compositions were integrated into an independent CYC and HA-SeSe-Ce6 co-delivery albumin nano-system (ABN@HA-SeSe-Ce6/CYC). The obtained nano-system presents good biocompatible, long circulation and effective tumor accumulation. After entering tumor microenvironment, CYC gradually releases to disrupt the ECM barrier to open the way for further penetration of HA-SeSe-Ce6. Subsequently, targeted tumor cell internalization and intracellular redox response release of Ce6 would achieve. Moreover, CYC could also make up the deficiency of Ce6 in hypoxia area, owing to its anti-tumor effect. Improved therapeutic efficacy was verified in a breast cancer cell line and tumor-bearing mice model.

34 Lipophilic derivatives of chlorin e6 as novel tools for photodynamic therapy

2012 ◽  
Vol 9 ◽  
pp. S12
Author(s):  
G.V. Ponomarev ◽  
M.N. Solovieva ◽  
N.O. Dugin ◽  
A.R. Mehtiev ◽  
A.Yu. Misharin
Keyword(s):  
Photodynamic Therapy ◽  
Chlorin E6 ◽  
Derivatives Of

Optical Properties of Photodynamic Therapy Drugs in Different Environments: The Paradigmatic Case of Temoporfin

2020 ◽  
Author(s):  
busenur Aslanoglu ◽  
Ilya Yakavets ◽  
Vladimir Zorin ◽  
Henri-Pierre Lassalle ◽  
Francesca Ingrosso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Optical Properties ◽  
Photodynamic Therapy ◽  
Minimally Invasive ◽  
Visible Light ◽  
Cancer Treatment ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Molecular Oxygen ◽  
Computational Tools

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.

Doxorubicin-loaded pH-responsive nanoparticles coated with chlorin e6 for drug delivery and synergetic chemo-photodynamic therapy

2020 ◽  
Vol 31 (19) ◽  
pp. 195103 ◽  
Author(s):  
Junlong Liang ◽  
Xiaokang Jin ◽  
Biling Chen ◽  
Jinhua Hu ◽  
Qianwei Huang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Chlorin E6 ◽  
Ph Responsive

scholarly journals Receptor-mediated Uptake of Folic Acid-functionalized Dextran Nanoparticles for Applications in Photodynamic Therapy

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 896 ◽  
Author(s):  
Kathrin Butzbach ◽  
Matthias Konhäuser ◽  
Matthias Fach ◽  
Denise Bamberger ◽  
Benjamin Breitenbach ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Folic Acid ◽  
Tumor Cells ◽  
Kb Cells ◽  
Promising Target ◽  
Nanoparticle System ◽  
Specific Uptake ◽  
Folic Acid Receptor ◽  
Targeted Photodynamic Therapy

In photodynamic therapy (PDT), photosensitizers and light are used to cause photochemically induced cell death. The selectivity and the effectiveness of the phototoxicity in cancer can be increased by a specific uptake of the photosensitizer into tumor cells. A promising target for this goal is the folic acid receptor α (FRα), which is overexpressed on the surface of many tumor cells and mediates an endocytotic uptake. Here, we describe a polysaccharide-based nanoparticle system suitable for targeted uptake and its photochemical and photobiological characterization. The photosensitizer 5, 10, 15, 20-tetraphenyl-21H, 23H-porphyrine (TPP) was encapsulated in spermine- and acetal-modified dextran (SpAcDex) nanoparticles and conjugated with folic acid (FA) on the surface [SpAcDex(TPP)-FA]. The particles are successfully taken up by human HeLa-KB cells, and a light-induced cytotoxicity is observable. An excess of free folate as the competitor for the FRα-mediated uptake inhibits the phototoxicity. In conclusion, folate-modified SpAcDex particles are a promising drug delivery system for a tumor cell targeted photodynamic therapy.

Overcoming drug resistance with functional mesoporous titanium dioxide nanoparticles combining targeting, drug delivery and photodynamic therapy

2018 ◽  
Vol 6 (46) ◽  
pp. 7750-7759 ◽  
Author(s):  
Zhaoming Guo ◽  
Kun Zheng ◽  
Zhenquan Tan ◽  
Ye Liu ◽  
Ziyin Zhao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Resistance ◽  
Titanium Dioxide ◽  
Photodynamic Therapy ◽  
Tumor Cells ◽  
Drug Delivery System ◽  
Delivery System ◽  
Titanium Dioxide Nanoparticles ◽  
Nano Drug Delivery ◽  
Targeting Strategy

We have designed a nano-drug delivery system ADH-1-HA-MTN, which can overcome the drug resistance of tumor cells based on an EMT cell targeting strategy in combination with PDT.

Sign in / Sign up

Export Citation Format

Share Document