Comparison of Cell Viability and Chemical Composition of Six Latest Generation Orthodontic Wires

Orthodontic wires are made of alloys containing different metals, including nickel. It is important to evaluate their biocompatibility prior to use, owing to their long-term use in patients. This in vitro study compared the cytotoxicity and chemical composition of six latest orthodontic wires: Fantasia®, Tanzo®, FLI®, NT3®, DuoForce®, and Gummetal®. The before-use group consisted of wires that were not used in the mouth, and the after-use group consisted of wires that were used in the mouth for two months. The wires were placed in contact with human gingival fibroblasts (HGF) for 72 h, and cytotoxicity was determined using the resazurin test. The chemical composition and surface characterisation were evaluated by spectrometry and scanning electron microscopy. The groups were compared using ANOVA and Kruskal–Wallis test. Only the FLI® wires produced a 36% reduction in HGF viability ( p < 0.05 ) and presented greater irregularities and loss of polymer structure. After-use wires showed a significant reduction in the percentage of nickel and the appearance of new elements (oxygen and carbon). Therefore, it can be concluded that no toxic ion release was noticed in this study. Rhodium-coated wires were more stable than PTFE-coated wires, and only the FLI® wires showed a slight cytotoxic effect.

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Human gingival fibroblasts proliferation and attachment to different novel implant-abutment materials: an in vitro study

10.26226/morressier.5ac383232afeeb00097a4910 ◽

2018 ◽

Author(s):

Ahmed Rozeik

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Human Gingival Fibroblasts ◽

Gingival Fibroblasts ◽

Implant Abutment

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The influence of cigarette smoke on colour stability and friction property of aesthetic orthodontic wires—In vitro study

International Orthodontics ◽

10.1016/j.ortho.2020.05.005 ◽

2020 ◽

Vol 18 (3) ◽

pp. 555-560

Author(s):

Flávio Mendonça Copello ◽

Lincoln Issamu Nojima ◽

Margareth Maria Gomes Souza ◽

Matheus Melo Pithon ◽

Antônio Carlos Oliveira Ruellas ◽

...

Keyword(s):

Cigarette Smoke ◽

In Vitro Study ◽

Vitro Study ◽

Colour Stability ◽

Friction Property ◽

Orthodontic Wires

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Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro

Clinical Oral Investigations ◽

10.1007/s00784-021-04130-0 ◽

2021 ◽

Author(s):

Jens Weusmann ◽

James Deschner ◽

Jean-Claude Imber ◽

Anna Damanaki ◽

Natalia D. P. Leguizamón ◽

...

Keyword(s):

Wound Healing ◽

Cell Function ◽

Nuclear Translocation ◽

In Vitro Study ◽

Human Gingival Fibroblasts ◽

Mrna Levels ◽

Gingival Fibroblasts ◽

Air Polishing ◽

Wide Range

Abstract Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.

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In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

The Angle Orthodontist ◽

10.2319/100714-717.1 ◽

2015 ◽

Vol 85 (6) ◽

pp. 920-926 ◽

Cited By ~ 2

Author(s):

Ricardo Carvalho Bueno ◽

Roberta Tarkany Basting

Keyword(s):

Chemical Composition ◽

In Vitro Study ◽

Chemical Components ◽

Control Group ◽

Osteoblast Proliferation ◽

Mini Implants ◽

Significant Difference ◽

Implant Anchorage ◽

Positive Effect

ABSTRACT Objective: To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods: Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n = 3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results: Two-way ANOVA showed no significant interaction between the factors studied (P = 0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P = .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P < .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions: Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

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Effect of different cleansers on the weight and ion release of removable partial denture: an in vitro study

Journal of Applied Oral Science ◽

10.1590/s1678-77572011000500008 ◽

2011 ◽

Vol 19 (5) ◽

pp. 483-487 ◽

Cited By ~ 17

Author(s):

Daniela N.B. Felipucci ◽

Letícia R. Davi ◽

Helena F.O. Paranhos ◽

Osvaldo L. Bezzon ◽

Rodrigo F. Silva ◽

...

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Ion Release ◽

Removable Partial Denture ◽

Partial Denture

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Effects of Cr(VI) long-term and low-dose action on mammalian antioxidant enzymes (an in vitro study)

Journal of Inorganic Biochemistry ◽

10.1016/j.jinorgbio.2003.12.014 ◽

2004 ◽

Vol 98 (3) ◽

pp. 490-496 ◽

Cited By ~ 20

Author(s):

Nino Asatiani ◽

Nelly Sapojnikova ◽

Marina Abuladze ◽

Tamar Kartvelishvili ◽

Nina Kulikova ◽

...

Keyword(s):

Antioxidant Enzymes ◽

Low Dose ◽

In Vitro Study ◽

Vitro Study

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High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes

Cellular Physiology and Biochemistry ◽

10.1159/000494481 ◽

2018 ◽

Vol 50 (3) ◽

pp. 973-986 ◽

Cited By ~ 4

Author(s):

Jung-Hwan Lew ◽

Koji Naruishi ◽

Yukari Kajiura ◽

Yasufumi Nishikawa ◽

Takahisa Ikuta ◽

...

Keyword(s):

High Glucose ◽

Gingival Crevicular Fluid ◽

In Vitro Study ◽

Inflammatory Factors ◽

Diabetic Patients ◽

Gingival Fibroblasts ◽

Cross Sectional ◽

Periodontal Tissues ◽

Severe Periodontitis

Background/Aims: Diabetic patients are susceptible to severe periodontitis, but the precise mechanism is not fully understood. Aim of this study was to explore the biological pathogenesis of severe periodontitis in diabetic patients focusing on the crosstalk of human gingival fibroblasts (HGFs) and macrophages. Methods: A total of 70 periodontitis patients with or without diabetes mellitus (DM) were enrolled, and the statistical relationships of diabetic conditions to the periodontal inflammatory parameters were examined by cross-sectional study. In in vitro study, HGFs cell line CRL-2014® (ATCC) and differentiated THP-1 macrophages were cultured with normal glucose (NG: 5.5 mM) or high glucose (HG: 25 mM) condition, and treated with indicated inflammatory factors such as calprotectin (CPT), interleukin (IL)-1β and IL-6. To examine the effects of HG on soluble IL-6 receptor (sIL-6R) production in THP-1 macrophages, the supernatants were collected and the sIL-6R levels were measured by ELISA. To examine the effects of HG on IL-1β or IL-6-induced matrix metalloproteinase (MMPs) production in HGFs, the supernatants were collected. Levels of MMP-1 and tissue inhibitor of MMP-1 (TIMP-1) were measured by ELISA. Finally, after conditioned medium (CM) from THP-1 macrophages cultured with NG or HG conditions was collected, HGFs were treated with the CM. The supernatants were collected 24 hours later and the levels of MMP-1 and TIMP-1 were measured. To examine the specific effects of IL-1β contained in CM on MMP-1 and TIMP-1 production in HGFs, IL-1 receptor antagonist (IL-1ra) was used. Results: There were statistical correlation between IL-1β and sIL-6R levels in gingival crevicular fluid (GCF) and HbA1c in periodontitis patients with DM (IL-1β: P=0.035, sIL-6R: P=0.040). HG and CPT significantly induced sIL-6R production in THP-1 macrophages. HG significantly enhanced IL-1β or IL-6/sIL-6R-induced MMP-1 production in HGFs. The increase of MMP-1 by both IL-1β and IL-6/sIL-6R was significantly inhibited by specific ERK or IκB inhibitors. Corresponding to the regulation of MMP-1 production, HG condition increased the phosphorylation of p44/42 MAPK and IκBα in HGFs treated with IL-1β or IL-6/sIL-6R. Finally, MMP-1 production in HGFs cultured with HG increased significantly by CM from THP-1 macrophages cultured with HG. The induction of MMP-1 by the CM from THP-1 macrophages cultured with HG was significantly inhibited by dose dependent of IL-1ra in HGFs cultured with HG. Conclusion: Diabetic conditions such as HG induce IL-1β and sIL-6R production from macrophages in inflammatory periodontal tissues and may exacerbate the periodontitis synergistically via MMP-1 production from HGFs.

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Marginal, Internal Fit and Microleakage of Zirconia Infrastructures: An In-Vitro Study

Operative Dentistry ◽

10.2341/10-107-lr1 ◽

2011 ◽

Vol 36 (1) ◽

pp. 72-79 ◽

Cited By ~ 10

Author(s):

L. Korkut ◽

H. S. Cotert ◽

H. Kurtulmus

Keyword(s):

Clinical Relevance ◽

In Vitro Study ◽

Manufacturing Technology ◽

Vitro Study ◽

Internal Fit ◽

Fitting Accuracy

Clinical Relevance Fitting accuracy and microleakage dominate prognostic covariates for the long-term durability of crown restorations. The fitting accuracy and microleakage potential of zirconia infrastructures might be influenced by manufacturing technology.

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In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid ofEchinococcus granulosus

Journal of Parasitology Research ◽

10.1155/2009/624919 ◽

2009 ◽

Vol 2009 ◽

pp. 1-7 ◽

Cited By ~ 17

Author(s):

Razika Zeghir-Bouteldja ◽

Manel Amri ◽

Saliha Aitaissa ◽

Samia Bouaziz ◽

Dalila Mezioug ◽

...

Keyword(s):

Nitric Oxide ◽

Hydatid Cyst ◽

Echinococcus Granulosus ◽

In Vitro Study ◽

No Production ◽

In Vitro Effects ◽

Nitric Oxide Metabolites

Hydatidosis is characterized by the long-term coexistence of larvaEchinococcus granulosusand its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity ofNO2−andONOO−24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated thatONOO−is more cytotoxic thanNO2−. In contrast,NO3−has no effect. These results suggest possible involvement ofNO2−andONOO−in antihydatic action and point the efficacy of these metabolites as scolicidal agents.

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