scholarly journals Comparison of Synergistic Effect of Nisin and Monolaurin on the Inactivation of Three Heat Resistant Spores Studied by Design of Experiments in Milk

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Souhir Kmiha ◽  
Chedia Aouadhi ◽  
Khouloud Aziza ◽  
Awatef Bejaoui ◽  
Abderrazak Maaroufi
Keyword(s):  
Synergistic Effect ◽  
Dairy Industry ◽  
Inhibitory Effect ◽  
Combined Effect ◽  
Bacterial Spores ◽  
Optimum Process ◽  
Uht Milk ◽  
Heat Resistant ◽  
Paenibacillus Sp ◽  
Bacillus Spores

Spore forming bacteria are special problems for the dairy industry. Heat treatments are insufficient to kill the spores. This is a continuously increasing problem for the industry, but we should be able to control it. In this context, we investigated the combined effect of nisin, monolaurin, and pH values on three heat resistant spores in UHT milk and distilled water and to select an optimal combination for the maximum spore inactivation. The inhibitory effect of nisin (between 50 and 200 IU/ml), monolaurin (ranging from 150 to 300 µg/ml), and pH (between 5 and 8) was investigated using a central composite plan. Results were analyzed using the response surface methodology (RSM). The obtained data showed that the inactivation of Bacillus spores by the combined effect of nisin-monolaurin varies with spore species, acidity, and nature of the medium in which the bacterial spores are suspended. In fact, Terribacillus aidingensis spores were more resistant, to this treatment, than Paenibacillus sp. and Bacillus sporothermodurans ones. The optimum process parameters for a maximum reduction of bacterial spores (∼3log) were obtained at a concentration of nisin >150 IU/ml and of monolaurin >200 µg/ml. The current study highlighted the presence of a synergistic effect between nisin and monolaurin against heat bacterial spores. So, such treatment could be applied by the dairy industry to decontaminate UHT milk and other dairy products from bacterial spores.

scholarly journals The CHARACTERIZATION OF SYNERGISTIC EFFECT OF CRILIN AND NANOCURCUMIN ON TREATMENT FOR 7, 12 DIMETHYLBENZ [A] ATHRACENE (DMBA)-INDUCED BREAST CANCER MICE

2018 ◽  
Vol 34 (1) ◽  
pp. 61
Author(s):  
Gia-Buu Tran
Keyword(s):  
Breast Cancer ◽  
Synergistic Effect ◽  
Tumor Volume ◽  
Ductal Carcinoma ◽  
Inhibitory Effect ◽  
Carcinoma Cells ◽  
Herbal Remedies ◽  
Surrounding Tissue ◽  
Neoplastic Disease

Breast cancer is the neoplastic disease which is characterized by unregulated ductal and lobular hyperplasia. Some herbal remedies have been researched and proved the inhibitory effect on breast cancer such as, Crilin-extracted from Cirnum latifolum and curcumin-isolated from Cucuma longa. However, the synergistic effect of crilin and nanocurcumin have not been studied yet. In this study, we established the mouse model of breast cancer induced by DMBA and evaluated the effectiveness of combination of crilin and nanocurcumin on treatment of breast cancer. After 12 weeks, co-administration of crilin and nanocurcumin inversed alteration of body weight, the number of erythrocytes and leukocytes induced by DMBA. Furthermore, the synergistic effect of crilin and nanocucumin on reduction of tumor volume was proven. Histological analysis revealed that co-administration of crilin and nanocurcumin inhibited invasion of mammary ductal carcinoma cells into surrounding tissue, recovered lobular cells structure, and diminished leukocyte composition. Thereby, the combination of crilin and nanocurcumin recovers immune system and prevent the development of breast cancer.

Effect of oxygen and bismuth impurities on the structural and physicochemical properties of nickel melts

2021 ◽  
Vol 1 ◽  
pp. 73-83
Author(s):  
K.S. Filippov ◽  
Keyword(s):  
Surface Tension ◽  
Physicochemical Properties ◽  
Pure Nickel ◽  
The State ◽  
Combined Effect ◽  
Negative Deviation ◽  
Raoult’S Law ◽  
Compression Effect ◽  
Heat Resistant ◽  
Effect Of Oxygen

There are practically no data in the literature on the combined effect of oxygen and bismuth impurities on the structural and physicochemical properties of nickel melts or heat-resistant alloys based on it. In the presented work, the state of pure nickel and nickel melts containing (0.005-0.01) bismuth and oxygen is considered in the process of refining from oxygen and bismuth impurities. The influence of these impurities on the structural and physicochemical properties was studied, which was evaluated by the parameters of the density and surface tension of the melts. In nickel melts with bismuth and oxygen, a compression effect and a negative deviation from Raoult’s law were observed. The effect of an increase in the surface tension of a nickel melt containing oxygen with an increase in the concentration of bismuth is found, which corresponds to the isotherm of desorption of excess substance from the surface into the bulk.

scholarly journals Effect of Drypetes gossweileri essential oil and irradiation treatments on inhibition and sensitivity of bacterial spores

2019 ◽  
Vol 26 (1) ◽  
pp. 65-77
Author(s):  
Steve Voundi Olugu ◽  
Maximilienne Ascension Nyegue ◽  
Iuliana Lazar ◽  
Marius Stamate ◽  
Dumitra Raducanu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Spore Germination ◽  
Sensory Analysis ◽  
Orange Juice ◽  
Bacterial Spores ◽  
Alpha Radiation ◽  
Minimal Inhibitory Concentrations ◽  
Ultraviolet C ◽  
Bacillus Spores

This work assesses the effect of Drypetes gossweileri essential oil on germination of Bacillus spores inoculated in orange juice and milk. We also report the capacity of the essential oil at 0.25, 0.5 and 1 µg/mL to induce sensitivity of spores to some irradiation treatments. The concentrations of essential oil were chosen after sensory analysis. The results show that the essential oil inhibited spores germination with minimal inhibitory concentrations inhibiting spore germination (MICg) of 10 µg/mL in the orange juice. In milk, the spores appeared to be less sensitive with MICg varying from 20 to 40 µg/mL. The sensory analysis revealed 2.5 µg/mL as the acceptable concentration of essential oil in orange juice. The essential oil at 1 µg/mL induced the sensitivity of spore of three Bacillus to alpha radiation of 101.4 Gy. Ultraviolet-C and microwave treatments in the presence of essential oil in general led to higher inactivation of spores up to 100% in some cases.

Inhibitory Effect of Glucocorticoids on Human-cloned 5-hydroxytryptamine3AReceptor Expressed in Xenopus  Oocytes

2004 ◽  
Vol 101 (3) ◽  
pp. 660-665 ◽  
Author(s):  
Takahiro Suzuki ◽  
Masahiro Sugimoto ◽  
Hideki Koyama ◽  
Takashi Mashimo ◽  
Ichiro Uchida
Keyword(s):  
Xenopus Oocytes ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Combined Effect ◽  
Induced Current ◽  
Direct Effects ◽  
Receptor Antagonists ◽  
Electrode Voltage ◽  
Direct Inhibitory Effect ◽  
Voltage Clamping

Background Methylprednisolone, dexamethasone, and other glucocorticoids have been found effective against nausea and vomiting induced by chemotherapy and surgery. Although the specific 5-hydroxytriptamine3 (5-HT3) receptor antagonists such as ondansetron and ramosetron are used as antiemetics, reports show that the use of 5-HT3 receptor antagonists with some glucocorticoids brings additional effects. Glucocorticoids are reported to be antiemetic. The effect of glucocorticoids on 5-HT3 receptor, however, has not been well characterized. This study was designed to examine whether dexamethasone and methylprednisolone had direct effects on human-cloned 5-HT3A receptor expressed in Xenopus oocytes. Methods Homomeric human-cloned 5-HT3A receptor was expressed in Xenopus oocytes. The authors used the two-electrode voltage-clamping technique to study the effect of methylprednisolone and dexamethasone on 5-HT-induced current. Results Both dexamethasone and methylprednisolone concentration-dependently attenuated 5-HT-induced current. Dexamethasone inhibited 2 microm 5-HT-induced current, which was equivalent to EC30 concentration for 5-HT3A receptor, with an inhibitory concentration 50% of 5.29 +/- 1.02 microm. Methylprednisolone inhibited 2 microm 5-HT-induced current with an inhibitory concentration 50% of 1.07 +/- 0.15 mm. The mode of inhibition with either dexamethasone or methylprednisolone was noncompetitive and voltage-independent. When administered together with the 5-HT3 receptor antagonists, ramosetron or metoclopramide, both glucocorticoids showed an additive effect on 5-HT3 receptor. Conclusion The glucocorticoids had a direct inhibitory effect on 5-HT3 receptors. The combined effect of glucocorticoids and the 5-HT3 receptor antagonists seems additive.

scholarly journals Synergistic Effect of Geranylgeranyltransferase Inhibitor, GGTI, and Docetaxel on the Growth of Prostate Cancer Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Bin Han ◽  
Naohiro Fujimoto ◽  
Mizuki Kobayashi ◽  
Tetsuro Matsumoto
Keyword(s):  
Prostate Cancer ◽  
Synergistic Effect ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Prostate Cancer Cell ◽  
Inhibitory Effect ◽  
Prostate Cancer Cells ◽  
Growth Inhibitory Effect ◽  
Prostate Cancer Treatment ◽  
Growth Inhibitory

Most advanced prostate cancers progress to castration resistant prostate cancer (CRPC) after a few years of androgen deprivation therapy and the prognosis of patients with CRPC is poor. Although docetaxel and cabazitaxel can prolong the survival of patients with CRPC, inevitable progression appears following those treatments. It is urgently required to identify better or alternative therapeutic strategies. The purpose of this study was to confirm the anti-cancer activity of zoledronic acid (Zol) and determine whether inhibition of geranylgeranylation in the mevalonate pathway could be a molecular target of prostate cancer treatment. We examined the growth inhibitory effect of Zol in prostate cancer cells (LNCaP, PC3, DU145) and investigated a role of geranylgeranylation in the anticancer activity of Zol. We, then, evaluated the growth inhibitory effect of geranylgeranyltransferase inhibitor (GGTI), and analyzed the synergy of GGTI and docetaxel by combination index and isobolographic analysis. Zol inhibited the growth of all prostate cancer cell lines tested in a dose-dependent manner through inhibition of geranylgeranylation. GGTI also inhibited the prostate cancer cell growth and the growth inhibitory effect was augmented by a combination with docetaxel. Synergism between GGTI and docetaxel was observed across a broad range of concentrations. In conclusion, our results demonstrated that GGTI can inhibit the growth of prostate cancer cells and has synergistic effect with docetaxel, suggesting its potential role in prostate cancer treatment.

scholarly journals Effects of a Chinese Herbal Medicine, Guan-Jen-Huang (Aeginetia indicaLinn.), on Renal Cancer Cell Growth and Metastasis

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Yu-Huei Liu ◽  
Meng-Luen Li ◽  
Meng-Yu Hsu ◽  
Ya-Yueh Pang ◽  
I-Ling Chen ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Renal Cancer ◽  
Cellular Proliferation ◽  
Synergistic Effects ◽  
Umbilical Vein ◽  
Chinese Herb ◽  
Inhibitory Effect ◽  
Chemotherapeutic Agents ◽  
Intercellular Adhesion Molecule ◽  
Migration And Invasion

Aeginetia indicaLinn. (Guan-Jen-Huang, GJH), a traditional Chinese herb, has the potential to be an immunomodulatory agent. The purpose of this study was to explore the effect of GJH in the treatment of renal cancer. Concentration-effect curves for the influence of GJH on cellular proliferation showed a biphasic shape. Besides, GJH had a synergistic effect on cytotoxicity when combined with 5-fluorouracil (5-FU)which may be due to the alternation of the chemotherapeutic agent resistance-related genes and due to the synergistic effects on apoptosis. In addition, treatment with GJH extract markedly reduced 786-O cell adherence to human umbilical vein endothelial cells (HUVECs) and decreased 786-O cell migration and invasion. In a xenograft animal model, GJH extract had an inhibitory effect on tumor cell-induced metastasis. Moreover, western blot analysis showed that the expression of intercellular adhesion molecule-1 (ICAM-1) in 786-O cells was significantly decreased by treatment with GJH extract through inactivation of nuclear factor-κB (NF--κB). These results suggest that GJH extract has a synergistic effect on apoptosis induced by chemotherapeutic agents and an inhibitory effect on cell adhesion, migration, and invasion, providing evidence for the use of water-based extracts of GJH as novel alternative therapeutic agents in the treatment of human renal cancer.

scholarly journals Fresh Garlic Extract has a Synergistic Effect with Antibiotics on ESBLs Producing E. coli Urinary Isolates

2019 ◽  
pp. 1-9
Author(s):  
Wedad M. Abdelraheem
Keyword(s):  
Synergistic Effect ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
Garlic Extract ◽  
Dilution Method ◽  
P Value ◽  
E Coli ◽  
Extended Spectrum Beta Lactamases ◽  
Antibacterial Susceptibility ◽  
Tract Infections

Aim: Extended spectrum beta-lactamases (ESBLs)-producing bacteria often exhibit a multidrug- resistant phenotype limiting the therapeutic options available to the clinician. This study was conducted to investigate the antibacterial susceptibility pattern of ESBL producing E. coli isolates and evaluation of the antibacterial activity of Garlic extract against it. Study Design: The study was carried out using antibiotics powder and Garlic extract. Place and Duration of Study: At the microbiology lab, Faculty of Medicine, Minia University between January 2017 to August 2018.  Methods: The study was carried on 55 ESBL producing E. coli isolates isolated from patients with urinary tract infections (UTI). Screening and confirmation for ESBL was done according to clinical and laboratory standard institute guidelines (CLSI) guidelines. The antibacterial sensitivity to a panel of antibiotics and Garlic was performed by tube dilution method. Results: The results of antibacterial susceptibility testing of ESBLs-producing E. coli isolates in our study showed a higher degree of resistance to the tested antibiotics. In the present study, Garlic showed an inhibitory effect on ESBLs- producing E. coli with concentrations ranging from 10-50 mg/ml and a mean of 30 mg/ml. Garlic extract produce a synergistic effect with all tested antibiotics against all tested ESBL producing E. coli isolates. A Significant decrease in ESBLs genes expression (SHV, TEM and CTX-m) was reported after treatment with Garlic extract (P value: ˂0.001). Conclusion: The combinations of antibiotics and Garlic may be more useful than individual agents, so we recommended that further researches should be undertaken to evaluate the combination of Garlic with antibiotics towards ESBL producing E. coli.

scholarly journals Synergistic effect of 5-fluorodeoxyuridine and quinazoline antifolates on murine leukemia self-cultured in vitro.

1997 ◽  
Vol 44 (4) ◽  
pp. 735-742
Author(s):  
M Balińska ◽  
I Szablewska ◽  
D Janiszewska ◽  
A Brzezińska ◽  
K Pawełczak
Keyword(s):  
Synergistic Effect ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Leukemia Cells ◽  
Parental Line ◽  
Prior Use ◽  
Thymidylate Synthesis ◽  
Combined Drugs ◽  
Murine Leukemia

The effect of thymidylate synthase inhibitors, fluorodeoxyuridine (FdUrd) and its two sulphonamide derivatives was examined in the culture of murine leukemia cells -- 5178Y (parental subline) and its fluorodeoxyuridine resistant subline 5178Y/F. A synergistic effect of the antimetabolites on cell survival was observed on exposure of the culture of either line to a slightly inhibitory concentration of FdUrd (1 nM) in combination with 2-desamino-2-methyl-10-propargyl-5,8-dideaza-pteroylsulphogluta mate or 2-desamino-2-methyl-10-propargyl-5,8-dideaza-pteroylsulphoglyci ne. This effect was accompanied by a marked reduction, in both cell lines of intracellular concentration of 5,10-methylenetetrahydro-pteroyl-polyglutamate, although its concentration in the resistant subline was 3 times as high as in the parental line. The inhibitory effect of combined drugs on the cellular pool of folates in 5178Y line depended also on the sequence of drug addition, whereas in the FdUrd resistant line this sequence was without any effect. The results obtained strongly suggest that under certain conditions inhibition of thymidylate synthesis by antifolates is intensified by a prior use of FdUrd.

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