In vitro Study of the Role of Human Neutrophil Enzymes on Root Caries Progression

2021 ◽  
pp. 1-9
Author(s):  
Camila A. Zamperini ◽  
Berdan Aydin ◽  
Herve Y. Sroussi ◽  
Ana Karina Bedran-Russo
Keyword(s):  
Human Neutrophil ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Root Surface ◽  
Host Immune System ◽  
Root Caries ◽  
Caries Progression ◽  
And Control

The role of the host immune system in caries progression is mainly speculative, and it is believed that it entails the enzymatic degradation of the dentin organic matrix. The aim of this study was to evaluate the proteolytic effect of human neutrophil enzymes on root caries progression. For this, specimens of bovine root dentin were divided into 4 groups (<i>n</i> = 30): caries (C), caries + neutrophils (C + N), no caries (Control), and no caries + neutrophils (Control + N). <i>Streptococcus mutans</i> biofilm (10<sup>5</sup> CFU/mL) was grown on the root surface to artificially induce root carious lesions (C and C + N groups). Specimens were then exposed to neutrophils (5 × 10<sup>6</sup> cells/mL) for 48 h (C + N and Control + N groups). Caries development and neutrophil exposures were repeated a 2nd and 3rd time. Caries depth (CD) and dentin demineralization (DD) were assessed by infiltration of rhodamine B using fluorescence microscopy. Collagen fibril ultrastructure was characterized under a polarized microscope with Picrosirius red staining. There were no significant differences (<i>p</i> &#x3e; 0.05) in CD and DD between the C and C + N groups for 1, 2, and 3 caries-neutrophil exposures. Immature collagen was significantly less present in the carious groups (C, <i>p</i> = 0.003; C + N, <i>p</i> = 0.01) than in the noncarious groups in the most superficial 200 µm. We thus concluded that human neutrophil enzymes did not influence short-term root caries progression, and immature collagen fibrils were more susceptible to degradation during <i>S. mutans</i>-induced root caries progression.

The Impact of the Demineralized Organic Matrix on the Effect of TiF4 Varnish on the Progression of Dentin Erosive Loss

2017 ◽  
Vol 51 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mariele Vertuan ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Analysis Data ◽  
Acid Ph ◽  
Bonferroni Test ◽  
And Control ◽  
The Impact

This in vitro study compared the effect of TiF4 varnish with that of NaF varnish, applied on pre-eroded bovine dentin samples, with respect to the progression of erosive loss, in the presence or absence of the demineralized organic matrix (DOM). One hundred and sixty bovine dentin samples were pre-eroded (0.1% citric acid, pH 2.5, 30 min). Half of the samples were subjected to the DOM removal (collagenase solution, 5 days). Samples with and without the DOM were treated according to the groups (n = 20 with DOM and 20 without DOM/group): TiF4 varnish (2.45% F), NaF varnish (2.45% F), placebo varnish (without fluoride) and control (no treatment). Thereafter, the treated samples were submitted to erosive challenges 4 × 90 s/day (0.1% citric acid, pH 2.5) during 7 days. Between the challenges, the samples were immersed in artificial saliva. The dentin erosive loss was measured using contact profilometry (µm, n = 15). Five dentin samples per group were prepared for energy-dispersive X-ray spectroscopy analysis. Data were compared using 2-way ANOVA/Bonferroni test (p < 0.05). Both fluoride varnishes were effective in reducing the erosive loss progression regardless of the dentin condition when compared to placebo varnish and control groups. Despite the fact that the TiF4 varnish was more effective than the NaF varnish for both dentin conditions (p < 0.001), its effect was significantly reduced in the absence of DOM (p < 0.05). It can be concluded that the TiF4 varnish is the best treatment in reducing the progression of dentin erosive loss (100%) in vitro, but its protective effect is more pronounced in the presence of DOM.

Inhibition of Root Caries Progression by an Antibacterial Adhesive

2005 ◽  
Vol 84 (1) ◽  
pp. 89-93 ◽  
Author(s):  
A. Kuramoto ◽  
S. Imazato ◽  
A.W.G. Walls ◽  
S. Ebisu
Keyword(s):  
Adhesive System ◽  
Root Surface ◽  
Culture Technique ◽  
Root Caries ◽  
Human Teeth ◽  
Caries Progression ◽  
Acid Gel ◽  
Caries Lesions ◽  
Demineralized Dentin

A dentin primer containing the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) has been shown to penetrate and kill the bacteria in artificially demineralized dentin. We hypothesized that an experimental adhesive system, which incorporates the MDPB-containing primer, would be effective in inhibiting the progression of root caries in vitro. Artificial caries lesions were prepared by either an acid-gel or a Streptococcus mutans culture technique on the roots of extracted human teeth. The progression of these lesions after the application of the experimental or proprietary adhesive system was examined. Further demineralization was completely prevented by the experimental adhesive system, while lesions managed with the proprietary materials showed limited ability to inhibit further demineralization. We conclude that the experimental adhesive system can inhibit the progression of root-surface caries in vitro, through a combination of its antimicrobial activity and sealing of the demineralized dentin.

scholarly journals Role of glyoxalases in wound healing process: an in vitro study

2021 ◽  
Vol 165 ◽  
pp. 39
Author(s):  
Francesca Lombardi ◽  
Silvano Santini ◽  
Paola Palumbo ◽  
Valeria Cordone ◽  
Virginio Bignotti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
In Vitro Study ◽  
Vitro Study ◽  
Wound Healing Process

scholarly journals SEM Analysis of Enamel Abrasion after Air Polishing Treatment with Erythritol, Glycine and Sodium Bicarbonate

Coatings ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 549 ◽  
Author(s):  
Bruna Sinjari ◽  
Gianmaria D’Addazio ◽  
Martina Bozzi ◽  
Manlio Santilli ◽  
Tonino Traini ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Sodium Bicarbonate ◽  
Air Flow ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Medical Systems ◽  
Air Polishing ◽  
And Control

The aim of this in vitro study was to evaluate the enamel surface topography after treatment with three air polishing powders: Glycine (A), erythritol (B), and sodium bicarbonate (C) (Air Flow Soft, Plus and Classic powders, EMS Electro Medical Systems S.A., Nyon, Switzerland). Fifteen extracted incisors were randomly divided into three groups of five teeth each, A, B and C, respectively. The teeth were blocked in plaster bases, washed, dried and half-covered with polytetrafluoroethylene strips before treatment. In this way, each half-treated dental element became test and control of itself. Comparative statistical analysis of Rq (geometric average of the deviations occurring in roughness profile) was performed. The scanning electron microscope (SEM) analysis showed different degrees of surface roughness between the groups, decreasing after treatment. In addition, a statistically significant reduction p < 0.05 was present in group C (Rq mean non-treated 108.17 µm, 95% CI: 97.29–124.01 and Rq mean treated 86.78 µm, 95% CI: 80.63–93.70). A decrease in surface roughness post-treatment was not observed in group A and B. Therefore, it may be concluded that the air flow powders tested herein can be used on dental enamel to reduce the surface roughness due to function and the action of dental therapies.

scholarly journals Genotoxicity and inflammatory potential of stainless steel welding fume particles: an in vitro study on standard vs Cr(VI)-reduced flux-cored wires and the role of released metals

2021 ◽  
Author(s):  
Sarah McCarrick ◽  
Valentin Romanovski ◽  
Zheng Wei ◽  
Elin M. Westin ◽  
Kjell-Arne Persson ◽  
...  
Keyword(s):  
Dna Damage ◽  
In Vitro Study ◽  
Human Monocyte ◽  
Welding Fumes ◽  
Welding Fume ◽  
Increased Risk ◽  
Underlying Mechanisms ◽  
Cored Wires

AbstractWelders are daily exposed to various levels of welding fumes containing several metals. This exposure can lead to an increased risk for different health effects which serves as a driving force to develop new methods that generate less toxic fumes. The aim of this study was to explore the role of released metals for welding particle-induced toxicity and to test the hypothesis that a reduction of Cr(VI) in welding fumes results in less toxicity by comparing the welding fume particles of optimized Cr(VI)-reduced flux-cored wires (FCWs) to standard FCWs. The welding particles were thoroughly characterized, and toxicity (cell viability, DNA damage and inflammation) was assessed following exposure to welding particles as well as their released metal fraction using cultured human bronchial epithelial cells (HBEC-3kt, 5–100 µg/mL) and human monocyte-derived macrophages (THP-1, 10–50 µg/mL). The results showed that all Cr was released as Cr(VI) for welding particles generated using standard FCWs whereas only minor levels (< 3% of total Cr) were released from the newly developed FCWs. Furthermore, the new FCWs were considerably less cytotoxic and did not cause any DNA damage in the doses tested. For the standard FCWs, the Cr(VI) released in cell media seemed to explain a large part of the cytotoxicity and DNA damage. In contrast, all particles caused rather similar inflammatory effects suggesting different underlying mechanisms. Taken together, this study suggests a potential benefit of substituting standard FCWs with Cr(VI)-reduced wires to achieve less toxic welding fumes and thus reduced risks for welders.

scholarly journals Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 3055
Author(s):  
Elisabetta Stanzani ◽  
Leire Pedrosa ◽  
Guillaume Bourmeau ◽  
Oceane Anezo ◽  
Aleix Noguera-Castells ◽  
...  
Keyword(s):  
Cellular Heterogeneity ◽  
In Vitro Assays ◽  
Rna Seq ◽  
Promising Target ◽  
Damage Response ◽  
Integrin Alpha 6 ◽  
And Control ◽  
Improve Patient

Therapeutic resistance after multimodal therapy is the most relevant cause of glioblastoma (GBM) recurrence. Extensive cellular heterogeneity, mainly driven by the presence of GBM stem-like cells (GSCs), strongly correlates with patients’ prognosis and limited response to therapies. Defining the mechanisms that drive stemness and control responsiveness to therapy in a GSC-specific manner is therefore essential. Here we investigated the role of integrin a6 (ITGA6) in controlling stemness and resistance to radiotherapy in proneural and mesenchymal GSCs subtypes. Using cell sorting, gene silencing, RNA-Seq, and in vitro assays, we verified that ITGA6 expression seems crucial for proliferation and stemness of proneural GSCs, while it appears not to be relevant in mesenchymal GSCs under basal conditions. However, when challenged with a fractionated protocol of radiation therapy, comparable to that used in the clinical setting, mesenchymal GSCs were dependent on integrin a6 for survival. Specifically, GSCs with reduced levels of ITGA6 displayed a clear reduction of DNA damage response and perturbation of cell cycle pathways. These data indicate that ITGA6 inhibition is able to overcome the radioresistance of mesenchymal GSCs, while it reduces proliferation and stemness in proneural GSCs. Therefore, integrin a6 controls crucial characteristics across GBM subtypes in GBM heterogeneous biology and thus may represent a promising target to improve patient outcomes.

scholarly journals TMIC-34. IN VITRO STUDY OF ROLE OF ASTROCYTIC S1P3 IN REGULATING BLOOD-BRAIN/TUMOR BARRIER PERMEABILITY

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi250-vi250
Author(s):  
Anurag N Paranjape ◽  
Brunilde Gril ◽  
Stephan Woditschka ◽  
Jeffrey Hanson ◽  
Xiaolin Wu ◽  
...  
Keyword(s):  
Brain Tumor ◽  
In Vitro Study ◽  
Vitro Study ◽  
Barrier Permeability ◽  
Blood Brain
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