Asma infantil: Efectos inmunomoduladores de los liofilizados bacterianos

2021 ◽  
pp. 1-2
Author(s):  
Patricia Ximena Solari
Keyword(s):  
T Lymphocytes ◽  
Immune Responses ◽  
Allergic Asthma ◽  
Respiratory Infections ◽  
Trial Registration ◽  
House Dust Mites ◽  
Dust Mites ◽  
Bacterial Lysate ◽  
Clinical Trial Registration ◽  
Registry Record

Background: Polyvalent Mechanical Bacterial Lysate (PMBL®) contains antigens of bacteria responsible for respiratory infections. PMBL® has been proven to reduce the number of respiratory infections, and in its use, immunological benefits have been seen in allergic patients. PMBL® activates both innate and specific immune responses. The lysate induces dendritic cells, T and B lymphocytes and IgA secretion, as well as the production of antibodies directed against administered bacterial antigens. Moreover, it increases the response against other bacteria and viruses. The immunologic mechanism of lysate’s action is not yet clearly determined. The objective of this study was to assess the effect of PMBL® on T cells in children with allergic asthma. Methods: This study was a part of the EOLIA study. Herein, 49 children with allergic asthma and house dust mites allergy were included: 21 in PMBL® and 28 in the Placebo group, both, drug and placebo were administered sublingually. The tests were done at baseline and 12 weeks after the last tablet intake. The lymphocytes CD45+, lymphocytes T CD3+, CD3+CD25+, CD3+CD69+, Th CD3+CD4+, CD4+CD25+, CD4+CD25+ high, CD4+CD69+, Treg CD4+CD25+FOXP3, Tc CD3+CD8+, CD8+CD25+, CD8+CD69+, NK-like T CD3+CD16+CD56+ and NK cells CD3−CD16+CD56+ were described. Results: At baseline, no significant differences between groups relative to blood count cells were observed, except for eosinophils. After 12 weeks, we observed an increase of T lymphocytes count. In addition, CD4+CD25+FOXP3+, CD8+ and CD3−CD16+CD56+ and (insignificantly) Th count increased. However, CD69+ and CD25+ subset of CD3+ significantly decreased. Conclusions: The EOLIA study demonstrated that PMBL® administration 10 days per month for 3 months changed the panel of T lymphocytes. Trial registration Clinical Trial Registration: This study was a part of the EOLIA (Efficacy Of mechanical bacterial Lysate In Allergic children), a clinical study NCT02541331. Frederic Durmont, MD Lallemand Pharma International AG. Date of registration 09/08/2013. URL of trial registry record: https://clinicaltrials.gov/ct2/show/study/NCT02541331.

scholarly journals Impact of Polyvalent Mechanical Bacterial Lysate on lymphocyte number and activity in asthmatic children: a randomized controlled trial

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Małgorzata Bartkowiak-Emeryk ◽  
Andrzej Emeryk ◽  
Jacek Roliński ◽  
Ewelina Wawryk-Gawda ◽  
Ewa Markut-Miotła
Keyword(s):  
T Lymphocytes ◽  
Allergic Asthma ◽  
Respiratory Infections ◽  
Controlled Trial ◽  
Trial Registration ◽  
House Dust Mites ◽  
Dust Mites ◽  
Bacterial Lysate ◽  
Clinical Trial Registration ◽  
Asthmatic Children

Abstract Background Polyvalent Mechanical Bacterial Lysate (PMBL®) contains antigens of bacteria responsible for respiratory infections. PMBL® has been proven to reduce the number of respiratory infections, and in its use, immunological benefits have been seen in allergic patients. PMBL® activates both innate and specific immune responses. The lysate induces dendritic cells, T and B lymphocytes and IgA secretion, as well as the production of antibodies directed against administered bacterial antigens. Moreover, it increases the response against other bacteria and viruses. The immunologic mechanism of lysate’s action is not yet clearly determined. The objective of this study was to assess the effect of PMBL® on T cells in children with allergic asthma. Methods This study was a part of the EOLIA study. Herein, 49 children with allergic asthma and house dust mites allergy were included: 21 in PMBL® and 28 in the Placebo group, both, drug and placebo were administered sublingually. The tests were done at baseline and 12 weeks after the last tablet intake. The lymphocytes CD45+, lymphocytes T CD3+, CD3+CD25+, CD3+CD69+, Th CD3+CD4+, CD4+CD25+, CD4+CD25+ high, CD4+CD69+, Treg CD4+CD25+FOXP3, Tc CD3+CD8+, CD8+CD25+, CD8+CD69+, NK-like T CD3+CD16+CD56+ and NK cells CD3−CD16+CD56+ were described. Results At baseline, no significant differences between groups relative to blood count cells were observed, except for eosinophils. After 12 weeks, we observed an increase of T lymphocytes count. In addition, CD4+CD25+FOXP3+, CD8+ and CD3−CD16+CD56+ and (insignificantly) Th count increased. However, CD69+ and CD25+ subset of CD3+ significantly decreased. Conclusions The EOLIA study demonstrated that PMBL® administration 10 days per month for 3 months changed the panel of T lymphocytes. Trial registration Clinical Trial Registration: This study was a part of the EOLIA (Efficacy Of mechanical bacterial Lysate In Allergic children), a clinical study NCT02541331. Frederic Durmont, MD Lallemand Pharma International AG. Date of registration 09/08/2013. URL of trial registry record: https://clinicaltrials.gov/ct2/show/study/NCT02541331.

scholarly journals A major house dust mite allergen disrupts the immunoglobulin E network by selectively cleaving CD23: innate protection by antiproteases.

1995 ◽  
Vol 182 (5) ◽  
pp. 1537-1544 ◽  
Author(s):  
C R Hewitt ◽  
A P Brown ◽  
B J Hart ◽  
D I Pritchard
Keyword(s):  
Cell Surface ◽  
Immune Responses ◽  
Allergic Asthma ◽  
House Dust ◽  
House Dust Mite ◽  
Immunoglobulin E ◽  
House Dust Mites ◽  
Dust Mites ◽  
Group I ◽  
Dust Mite

Asthma is a chronic life-threatening disease of worldwide importance. Although allergic asthma and related atopic conditions correlate strongly with immune sensitization to house dust mites, it is unclear why antigens from mites provoke such powerful allergic immune responses. We have characterized the protease activity of Der p I, the group I protease allergen of the house dust mite Dermatophagoides pteronyssinus, and here report that it cleaves the low-affinity immunoglobulin (Ig) E Fc receptor (CD23) from the surface of human B lymphocytes. Der p I selectively cleaves CD23 and has no effect on the expression of any other B cell surface molecules tested. We speculate that this loss of cell surface CD23 from IgE-secreting B cells may promote and enhance IgE immune responses by ablating an important feedback inhibitory mechanism that normally limits IgE synthesis. Furthermore, since soluble CD23 is reported to promote IgE production, fragments of CD23 released by Der p I may directly enhance the synthesis of IgE. alpha 1-Antiprotease, a pulmonary antiprotease, is also shown to inhibit the cleavage of CD23 by Der p I. This may be significant in the etiopathogenesis of asthma, because other indoor pollutants associated with asthma are known to potently inhibit this antiprotease. These data suggest that the proteolytic activity of Der p I, the group I allergen of the house dust mite D. pteronyssinus, is mechanistically linked to the potent allergenicity of house dust mites. Furthermore, inhibition of Der p I by alpha 1-antiprotease suggests a mechanism by which confounding factors, such as tobacco smoke, may act as a risk factor for allergic asthma.

scholarly journals Sensitivity to House Dust Mites Allergens in Patients with Allergic Asthma in Erzincan Province, Turkey

2017 ◽  
Vol 41 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Erhan Zeytun ◽  
Salih Dogan ◽  
Fatih Ozcicek ◽  
Edhem Unver
Keyword(s):  
Allergic Asthma ◽  
House Dust ◽  
House Dust Mites ◽  
Dust Mites

scholarly journals Environmental Allergens House Dust Mites-induced Asthma Causes Ferroptosis in the Lungs

2021 ◽  
Author(s):  
Weifeng Tang ◽  
Ming Dong ◽  
Fangzhou Teng ◽  
Jie Cui ◽  
Xueyi Zhu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Allergic Asthma ◽  
House Dust ◽  
Inflammatory Cell ◽  
Morphological Changes ◽  
Mucus Secretion ◽  
House Dust Mites ◽  
Control Group ◽  
Dust Mites ◽  
Cell Counts

Abstract Background: Studies have indicated that allergens such as house dust mites (HDM) in the environment could induce allergic asthma. Ferroptosis is a newly discovered regulatory cell death characterized by aberrant lipid peroxidation and accumulation of reactive oxygen species (ROS) in cells. However, whether ferroptosis participates in the pathological progress of asthma remains to be elucidated. In this study, we used a HDM-induced mouse asthma model to determine the effect of HDM exposure on allergic asthma and the underlying mechanisms. Methods: Female BALB/c mice were intranasally exposed to HDM to induce allergic asthma. Airway hyperresponsiveness (AHR), lung inflammation and mucus secretion, IgE and cytokine levels as well as inflammatory cell counts in bronchalveolar lavage fluid (BALF) were investigated. In addition, the morphological changes of mitochondria, ROS, glutathione (GSH) levels and changes in ferroptosis pathway proteins in the lung were also determined. Results: HDM exposure increased AHR significantly, and enhanced inflammatory cell infiltration and mucus secretion around the airways. Furthermore, elevated IgE level in BALF, lung eosinophilia, and a concomitant increase in IL-13 and IL-5 in BALF were observed. HDM inhalation increased ROS and decreased GSH level in the lung. HDM inhalation induced dysmorphic small mitochondria with decreased crista, as well as condensed, ruptured outer membranes. Western blot analysis demonstrated that activity of glutathione peroxidase 4 (GPX4) and catalytic subunit solute carrier family 7 member 11 (SLC7A11) decreased significantly, and protein expression of acyl-CoA synthetase long-chain family member 4 (ACSL4) and 15 Lipoxygenase 1 (15-LO1) upregulated prominently compared with mice in normal control group. Conclusions: These in all indicated that the AHR, airway inflammation, lipid peroxidation and ROS level increased in HDM-induced asthma, and HDM inhalation caused ferroptosis in the lungs, which helped to form a better understanding of the pathogenesis of allergic asthma and targeted treatment strategies.

scholarly journals Impact of house dust mite-driven asthma on children’s school performance and activity

2021 ◽  
Author(s):  
Catalina Gómez ◽  
Judit Barrena ◽  
Vanesa García-Paz ◽  
Ana M. Plaza ◽  
Paula Crespo ◽  
...  
Keyword(s):  
Children And Adolescents ◽  
Allergic Asthma ◽  
House Dust ◽  
Allergen Immunotherapy ◽  
House Dust Mites ◽  
Dust Mites ◽  
Activity Impairment ◽  
Daily Lives ◽  
School Work ◽  
Clinical Benefits

AbstractEvidence regarding asthma’s impact on children’s daily lives is limited. This prospective and cross-sectional, observational, multicenter study assessed school/work and activity impairment in children and adolescents with allergic asthma and their caregivers and allergen immunotherapy (AIT) effects. Included patients were schooled children and adolescents (5 to 17 years) with allergic asthma due to house dust mites (HDM). Impairment of school/work (i.e., absenteeism and presenteeism) and activity was measured in patients and their caregivers using the Work Productivity Impairment Questionnaire plus Classroom Impairment Questions: Allergy Specific (WPAI + CIQ:AS). HDM allergic patients with school impairment received subcutaneous AIT with a MicroCrystalline Tyrosine-associated allergoid. WPAI + CIQ:AS and effectiveness variables were compared between baseline and 1-year post-AIT. Of the 113 patients included, 59 (52.2%) and 51 (45.1%) showed school and activity impairment, respectively, missing a mean (SD) of 37.6 (24.4) % and 42.6 (25.6) % of school and activity time, respectively. Twenty-six (23%) caregivers reported activity impairment and, of the 79 (69.9%) employed, 30 (38%) reported work impairment. Of the 65 patients with school/activities impairment, 41 (63.1%) received AIT, of which 21 (51.2%) completed 1 year of treatment. Effectiveness variables and WPAI + CIQ:AS significantly improved: Mean (SD) school impairment decreased from 39.7 (26.7) to 2.1 (7.1) % (p < 0.001) and activity impairment from 46.2 (34.6) to 1.4 (3.6) % (p < 0.001).Conclusion: Allergic asthma due to HDMs results in school/work and activity impairment in children and adolescents and their caregivers. One year of AIT provided clinical benefits and reduced school and activity impairment. What is Known:• Allergic asthma impairs children’s school performance and daily activities.• Allergen immunotherapy modifies allergic disease course and ameliorates its symptoms. What is New:• Asthma symptoms due to allergy to house dust mites impair children’s school attendance and productivity and daily activity and their caregivers’ work performance and daily lives.• Allergen immunotherapy with a house dust mite MicroCrystalline Tyrosine (MCT)-associated allergoid seems to provide clinical benefits, associated with decreased school and activity impairment, supporting it as an effective treatment option.

scholarly journals Precision Medicine in House Dust Mite-Driven Allergic Asthma

2020 ◽  
Vol 9 (12) ◽  
pp. 3827
Author(s):  
Ibon Eguiluz-Gracia ◽  
Francisca Palomares ◽  
Maria Salas ◽  
Almudena Testera-Montes ◽  
Adriana Ariza ◽  
...  
Keyword(s):  
Allergic Asthma ◽  
House Dust ◽  
Inhaled Corticosteroids ◽  
Current Knowledge ◽  
Allergen Challenge ◽  
Pharmaceutical Products ◽  
House Dust Mites ◽  
Dust Mites ◽  
Bronchial Allergen Challenge

House dust mites (HDMs) are the allergenic sources most frequently involved in airway allergy. Nevertheless, not every sensitized patient develops respiratory symptoms upon exposure to HDM, and there is a clinical need to differentiate allergic asthmatics (AAs) from atopic non-allergic asthmatics with HDM sensitization. This differentiation sometimes requires in vivo provocations like the bronchial allergen challenge (BAC). Interestingly, recent data demonstrate that non-atopic patients with asthma can also develop positive BAC results. This novel phenotype has been termed local allergic asthma (LAA). The interest in identifying the allergic triggers of asthma resides in the possibility of administering allergen immunotherapy (AIT). AIT is a disease-modifying intervention, the clinical benefit of which persists after therapy discontinuation. Recently, new modalities of sublingual tablets of HDM immunotherapy registered as pharmaceutical products (HDM-SLIT tablets) have become commercially available. HDM-SLIT tablets have demonstrated a robust effect over critical asthma parameters (dose of inhaled corticosteroids, exacerbations, and safety), thus being recommended by international guidelines for patients with HDM-driven AA. In this review, we will summarize the current knowledge on the phenotype and endotype of HDM-driven AA, and LAA, address the difficulties for BAC implementation in the clinic, and discuss the effects of AIT in AA and LAA.

scholarly journals AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites

2015 ◽  
Vol 112 (22) ◽  
pp. E2891-E2899 ◽  
Author(s):  
Lark Kyun Kim ◽  
Rimpei Morita ◽  
Yasushi Kobayashi ◽  
Stephanie C. Eisenbarth ◽  
Chun Geun Lee ◽  
...  
Keyword(s):  
Immune Responses ◽  
House Dust ◽  
House Dust Mites ◽  
Mouse Lung ◽  
Dust Mites ◽  
Dust Mite ◽  
Caspase 7 ◽  
Acidic Mammalian Chitinase ◽  
Subsequent Activation

Chitinases are enzymes that cleave chitin, a component of the exoskeleton of many organisms including the house dust mite (HDM). Here we show that knockin mice expressing an enzymatically inactive acidic mammalian chitinase (AMCase), the dominant true chitinase in mouse lung, showed enhanced type 2 immune responses to inhaled HDM. We found that uncleaved chitin promoted the release of IL-33, whereas cleaved chitin could be phagocytosed and could induce the activation of caspase-1 and subsequent activation of caspase-7; this results in the resolution of type 2 immune responses, probably by promoting the inactivation of IL-33. These data suggest that AMCase is a crucial regulator of type 2 immune responses to inhaled chitin-containing aeroallergens.

scholarly journals Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma

2019 ◽  
Vol 11 (2) ◽  
pp. 194-9
Author(s):  
Cityta Putri Kwarta ◽  
Heri Wibowo ◽  
Yordan Khaedir ◽  
Iris Rengganis ◽  
Hanny Siti Nuraeni
Keyword(s):  
Allergic Asthma ◽  
Interleukin 10 ◽  
House Dust Mites ◽  
Atopic Asthma ◽  
Assay Method ◽  
Dust Mites ◽  
Interleukin 13 ◽  
Ifn Γ ◽  
Cellular Immune

BACKGROUND: Allergic asthma is a degenerative atopic disease caused by allergic or hypersensitivity type-1. More than 50% of people with allergic asthma are caused by the presence of house dust mites (HDMs) allergens.METHODS: The cellular immunity response was evaluated through a peripheral blood mononuclear cell (PBMC) culture isolated from blood, using the ficoll gradient technique. Subjects were atopic asthma groups and non-atopic asthma groups. PBMC from each subject cultured was stimulated with HDMs allergen, then incubated in a CO2 5% incubator, 37o C for 72 hours. With the multiplex assay method, interferon (IFN)-γ, interleukin (IL)-13 and IL-10 were measured, meanwhile indoleamine 2,3-dioxygenase level (IDO) was measured by the enzyme-linked immunosorbent assay (ELISA) sandwich methods.RESULTS: The IFN-γ production in the supernatant of PBMC cultures was stimulated by phytohemagglutinin (PHA), Roswell Park Memorial Institute (RPMI) medium and allergens. The IFN-γ production in allergen-stimulated supernatants showed higher level of IFN-γ in the nonatopic group (4,681,455±3,434,851) than atopic group (4,363,300±2,067,941) even though it was not statistically significant (p=0.078). There were no differences between the mean of IL-13 production in atopic asthma group and non-atopic group. The IL-10 production in allergenstimulated supernatants was shown to be higher in nonatopic group and were statistically significantly different (p=0.015). The IDO production in allergen-stimulated supernatants was shown to be higher in the non-atopic group (272,231±269,564) than in the actopic group (13,273±400), and it was significantly different (p=0.007).CONCLUSION: Cellular immune profile of subjects with allergic asthma to Dermatophagoides pterronyssinus (Der p) is characterized by a type-2 inflammatory response that is dominant compared to type-1 inflammation (higher IL-13 ratio compared to IFN-γ) and to the role of anti-inflammation (higher IL-13 ratio compared to IL-10). The decline in IDO production in allergic asthma subjects to Der p is thought to be related to the low cellular immune response in expressing IFN-γ compared to IL-13.KEYWORDS: interleukin-13, interleukin-10, IDO, PBMC, asthma

MITE ASTHMA IN CHILDHOOD: A STUDY OF THE RELATIONSHIP BETWEEN EXPOSURE TO HOUSE DUST MITES AND DISEASE ACTIVITY

PEDIATRICS ◽  
1994 ◽  
Vol 94 (2) ◽  
pp. 250-251
Author(s):  
Martin Sachs
Keyword(s):  
House Dust ◽  
Respiratory Infections ◽  
Mite Allergen ◽  
House Dust Mites ◽  
Dust Mites ◽  
Asthmatic Children ◽  
Allergic Asthmatic ◽  
Viral Respiratory Infections ◽  
The Relationship ◽  
Viral Respiratory

When mite allergic asthmatic children are exposed to high levels of mite allergen, the number of mites in the mattress dust does not correlate with increased symptoms. The authors further suggested that other factors (eg, viral respiratory infections) are more likely to be associated with increased symptoms.

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