The effect of various fluoride products on dentine lesions during pH-cycling

2021 ◽  
Author(s):  
Marwa M. Alhothali ◽  
Rob A.M. Exterkate ◽  
Maxim Lagerweij ◽  
A.J.P. Van Strijp ◽  
Mark J. Buijs ◽  
...  
Keyword(s):  
Fluoride Concentration ◽  
Outer Zone ◽  
Subsequent Removal ◽  
Treatment Control ◽  
X Ray ◽  
Superior Effect ◽  
Ph Cycling ◽  
Topical Fluoride ◽  
Calcium Loss

This study compared the effect of topically applied fluoride products on dentine lesions in an in vitro experiment. Demineralized bovine dentine specimens were treated once with either SDF solution (35,400 ppm F), NaF varnish (22,600 ppm F), TiF4 solution (9,200 ppm F), SnF2 gel (1,000 ppm F), no treatment (control) or preserved as baseline lesions. After the application and subsequent removal of the fluoride products, the specimens were subjected to pH-cycling. Calcium loss and uptake in the de- and remineralization buffers were assessed daily. Fluoride release into the buffers was analyzed on day 1, 2, 3, 5, 8 and 13. After the pH-cycling period, mineral distribution throughout the lesion depth was analyzed using transversal microradiography (TMR). X-ray energy-dispersive spectroscopy (EDS) examined the deposition of silver, titanium and tin after application of SDF, TiF4, and SnF2, respectively. Overall, calcium loss and uptake analysis in the de- and remineralization buffers revealed that the SDF product was the most effective in inhibiting lesion progression, followed by the TiF4, NaF, and SnF2 products. Fluoride analysis disclosed a steep reduction of the amount of fluoride released into de- and remineralization buffers with time. The fluoride effects on de- and remineralization continued beyond the days that fluoride was released into the buffers. TMR analysis showed significant remineralization in the outer zone of the dentine lesions for all fluoride products, with SDF giving hyper-mineralization in this zone. In the inner zone, lesions developed in all fluoride groups, with the smallest in the SDF group. EDS showed silver and titanium deposition in depth up to 85 μm and 8 μm, respectively, while no tin deposition was observed. The silver in the dentine lesions did not contribute significantly to the density of the TMR profiles in the SDF group. In conclusion, all topical fluoride products protected the dentine lesions against lesion progression, but at different degrees. SDF showed a superior effect in protection against further demineralization and enhancement of remineralization. This was probably attributed to its fluoride concentration that was the highest among the fluoride products.

Effect of Heavy Metals Contamination from Cigarette Smoke on Sound and Caries-Like Enamel

2018 ◽  
Vol 24 (6) ◽  
pp. 762-767 ◽  
Author(s):  
Jéssica D. Theobaldo ◽  
Waldemir F. Vieira-Junior ◽  
Anderson Catelan ◽  
Maria do Carmo A. Mainardi ◽  
Orlando A. Ysnaga ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cigarette Smoke ◽  
Surface Microhardness ◽  
Treatment Control ◽  
Cross Sectional ◽  
X Ray ◽  
Heavy Metals Contamination ◽  
Heavy Metal Deposition ◽  
Ph Cycling

AbstractIn this study, we sought to evaluate the influence of cigarette smoke and pH cycling on the chemical composition and surface/cross-sectional enamel microhardness. A total of 40 dental blocks obtained from bovine incisors were divided into four groups (n=10): no treatment (control); exposure to cigarette smoke (CS); exposure to pH cycling (PC); and exposure to cigarette smoke and pH cycling (CS-PC). The samples were analyzed by synchrotron radiation micro X-ray fluorescence, bench mode X-ray fluorescence, as well as surface microhardness (SMH) and cross-sectional microhardness (CSMH) testing. The SMH results were submitted to analysis of variance (ANOVA) and Tukey’s test. The CSMH results were evaluated using split-plot ANOVA and Tukey’s test. A high amount of Cd and Pb and traces of Ni and As were observed in enamel and dentin after exposure to cigarette smoke (CS and CS-PC). The SMH and CSMH of CS were statistically higher when compared with the control. The PC and CS-PC showed lower SMH and CSMH. We conclude that exposure to cigarette smoke promoted heavy metal deposition in enamel/dentin. In addition, it increased the enamel microhardness but did not promote a protective effect on the in vitro development of caries. The clinical significance of this work is that there is significant bioaccumulation of heavy metals from cigarette smoke on the surface and in the enamel and dentin.

The Effects of Gic Restorations on Enamel and Dentin Demineralization and Remineralization

1995 ◽  
Vol 9 (4) ◽  
pp. 384-388 ◽  
Author(s):  
J.M. Ten Cate ◽  
M.J. Buus ◽  
J.J.M. Damen
Keyword(s):  
Calcium Uptake ◽  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Mineral Balance ◽  
Oral Environment ◽  
Ph Cycling ◽  
Calcium Loss ◽  
Ionomer Cement

Glass-ionomer cement (GIC) restorations release fluoride after placement in the oral environment. This is assumed to be one of the reasons for the decreased prevalence of secondary caries. In the current studies, the effects of GIC restorations on demineralization and remineralization of enamel and dentin lesions were studied, in an in vitro and an intra-oral model, respectively. The in vitro pH-cycling study revealed that the excessive fluoride release of the freshly placed restorations upset the (commonly observed) mineral balance between the specimens and the de/remineralizing solutions. Anomalous patterns of calcium uptake by the specimens (with GIC) during the demineralization phase and calcium loss during the remineralization phase were observed. This was hypothetically explained from the behavior of a surface coating on the GIC restorations, which was observed with SEM. The intra-oral studies showed, after three months in situ, hypermineralization of dentin close to or in contact with the restoration. It is concluded that the fluoride released from GIC or the decreased permeability as a result of hypermineralization causes lower susceptibility of the dentin of the cavity walls.

scholarly journals Chemical, Microstructural and Morphological Characterisation of Dentine Caries Simulation by pH-Cycling

Minerals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 5
Author(s):  
Juan Sebastián Zuluaga-Morales ◽  
María Victoria Bolaños-Carmona ◽  
Carolina Cecilia Cifuentes-Jiménez ◽  
Pedro Álvarez-Lloret
Keyword(s):  
Microstructural Properties ◽  
Third Molars ◽  
X Ray Diffraction ◽  
X Ray ◽  
In Vitro Simulation ◽  
Ph Cycling ◽  
Residual Caries ◽  
Microscopy Techniques

In vitro simulation of natural caries is of great importance in dental research for the development of more effective clinical treatments. The pH-cycling (pHc) procedure consists of a dynamic caries process with alternating de-remineralisation periods. The current research aims to evaluate the effects of the pHc procedure on mineral dentine properties in comparison with sound dentine and natural residual caries. For this purpose, dentine slices from human third molars were submitted to cycling periods of 14 and 28 days. The chemical composition, morphological and microstructural properties of the dentine samples were examined by infrared and Raman spectroscopies, X-ray diffraction, and scanning electron microscopy techniques. In addition, the depth of the demineralisation front was evaluated by Masson’s trichrome (MT) staining. The results showed that the pHc procedure led to notable changes in the mineral composition and the crystalline characteristics with respect to sound dentine and some extent to natural caries. The MT results revealed that pHc 28 yields a deeper lesion than pHc 14, simulating potential progression of natural caries. The results of this study provide a better understanding of the mechanisms of demineralisation that could occur in an in vivo environment and provide a standardised substrate similar to natural residual caries.

Effect of Fluoride Concentration on Remineralization of Carious Enamel an in vitro pH-Cycling Study

1990 ◽  
Vol 24 (3) ◽  
pp. 174-180 ◽  
Author(s):  
F.A. Damato ◽  
R. Strang ◽  
K.W. Stephen
Keyword(s):  
Fluoride Concentration ◽  
Ph Cycling

scholarly journals Effect of Galla Chinensis on Remineralization of Early Dentin Lesion

2020 ◽  
Vol 14 (04) ◽  
pp. 651-656
Author(s):  
Hend Mahmoud Abdel-Azem ◽  
Ahmed Fawzy Abo Elezz ◽  
Rehab Khalil Safy
Keyword(s):  
Treatment Time ◽  
Analysis Data ◽  
Environmental Scanning ◽  
X Ray ◽  
Parametric Data ◽  
Significant Difference ◽  
Ph Cycling ◽  
The Mean ◽  
Post Hoc

Abstract Objective This study aimed to investigate the efficacy of the Galla chinensis extract (GCE) on early dentin lesion remineralization. Materials and Methods Seventy-two dentin specimens were divided into three groups according to the treatment solution. In group S1, specimens were treated with GCE; in group S2, the specimens were treated with sodium fluoride (NaF); meanwhile, specimens of group S3 were treated with distilled water (DW). Each group was further subdivided into two subgroups according to the treatment time (1 minute and 5 minutes). An in vitro pH-cycling model for 12 days was done. Subsequently, surface microhardness (SMH) of the specimens, elemental analysis, and their micromorphological appearance were evaluated. Statistical Analysis Data were statistically analyzed. One-way analysis of variance was used to compare numerical (parametric) data between more than two separate groups followed by post hoc Tukey. Results There was no significant difference between the mean SMH of dentin between NaF and GCE groups. Regarding the time, the 5-minute treatment with NaF and DW groups recorded higher mean SMH value of dentin than the 1-minute treatment group. Meanwhile, for GCE groups, the 1-minute immersion recorded higher mean SMH value than the 5-minute immersion without any significant difference between them. The microhardness results were confirmed by environmental scanning electron microscope and energy dispersive X-ray analysis results. Conclusion GCE could be used as an effective alternative for dentin remineralization.

scholarly journals Effect of Trimetaphosphate and Fluoride Association on Hydroxyapatite Dissolution and Precipitation In Vitro

2014 ◽  
Vol 25 (6) ◽  
pp. 479-484 ◽  
Author(s):  
Alberto Carlos Botazzo Delbem ◽  
José Antonio Santos Souza ◽  
Ana Carolina Soares Fraga Zaze ◽  
Eliana Mitsue Takeshita ◽  
Kikue Takebayashi Sassaki ◽  
...  
Keyword(s):  
Calcium Fluoride ◽  
Calcium Concentration ◽  
Ph Value ◽  
X Ray Diffraction ◽  
X Ray ◽  
Sodium Trimetaphosphate ◽  
Hydroxyapatite Powder ◽  
Calcium Phosphorus ◽  
Ph Cycling

The present study analyzed the action of sodium trimetaphosphate (TMP) and/or fluoride on hydroxyapatite. Hydroxyapatite powder was suspended in different solutions: deionized water, 500 µg F/mL, 1,100 µg F/mL, 1%TMP, 3%TMP, 500 µg F/mL plus 1%TMP and 500 µg F/mL plus 3%TMP. The pH value of the solutions was reduced to 4.0 and after 30 min, raised to 7.0 (three times). After pH-cycling, the samples were analyzed by X-ray diffraction and infrared spectroscopy. The concentrations of calcium fluoride, fluoride, calcium and phosphorus were also determined. Adding 1% or 3% TMP to the solution containing 500 µg F/mL produced a higher quantity of calcium fluoride compared to samples prepared in a 1,100 µg F/mL solution. Regarding the calcium concentration, samples prepared in solutions of 1,100 µg F/mL and 500 µg F/mL plus TMP were statistically similar and showed higher values. Using solutions of 1,100 µg F/mL and 500 µg F/mL plus TMP resulted in a calcium/phosphorus ratio close to that of hydroxyapatite. It is concluded that the association of TMP and fluoride favored the precipitation of a more stable hydroxyapatite.

scholarly journals In VitroProperties of Orthodontic Adhesives with Fluoride or Amorphous Calcium Phosphate

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Clara Ka Wai Chow ◽  
Christine D. Wu ◽  
Carla A. Evans
Keyword(s):  
Calcium Phosphate ◽  
Bacterial Adhesion ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Lesion Formation ◽  
X Ray ◽  
Orthodontic Adhesives ◽  
Ph Cycling ◽  
Phosphorous Content

Thisin vitrostudy evaluated the efficacy of orthodontic adhesives with fluoride or amorphous calcium phosphate (ACP) in reducing bacterial adhesion and enamel demineralization. Forty human premolars each sectioned buccolingually into three parts were bracketed with control resin (Transbond XT) or adhesives containing ACP (Aegis Ortho) or fluoride (QuickCure). Artificial lesions induced by pH cycling were examined by X-ray photoelectron spectrophotometry (XPS) and polarized light microscopy (PLM). After 28 days, Aegis Ortho demonstrated the lowest calcium and phosphorous content by XPS analysis. After 42 days, reductions in lesion depth areas were 23.6% for Quick Cure and 20.3% for Aegis Ortho (P<0.05). In the presence of 1% sucrose, adhesion ofStreptococcus mutansto Aegis Ortho and Quick Cure was reduced by 41.8% and 37.7% (P<0.05) as compared to Transbond XT. Composites containing ACP or fluoride reduced bacterial adherence and lesion formation as compared to a composite without ACP or fluoride.

scholarly journals Comparison between Fluoride and Nano-hydroxyapatite in Remineralizing Initial Enamel Lesion: An in vitro Study

2018 ◽  
Vol 19 (3) ◽  
pp. 306-312 ◽  
Author(s):  
Issa Daas ◽  
Sherine Badr ◽  
Essam Osman
Keyword(s):  
Dental Fluorosis ◽  
Fluoride Concentration ◽  
Fluoride Varnish ◽  
Permanent Teeth ◽  
Control Group ◽  
Representative Specimen ◽  
Initial Caries ◽  
Significant Difference ◽  
Ph Cycling

ABSTRACT Aim The aim of this study was to compare the effectiveness of nano-hydroxyapatite (nano-HAP) paste and fluoride varnish in remineralizing initial enamel lesion in young permanent teeth and their ability to resist secondary caries under dynamic pH cycling quantitatively and qualitatively. Materials and methods Initial caries-like lesions were artificially developed on 45 specimens. Specimens were divided into three groups: (1) Control (without treatment), (2) fluoride varnish (3M ESPE), and (3) nano-HAP paste (Desensibilize Nano P). The nano-HAP paste was applied twice separated by one pH cycle, and the varnish was applied only once followed by 7 days of pH cycling. All specimens were examined using DIAGNOdent® pen (KaVo, Germany), and a representative specimen was randomly selected from each group for qualitative evaluation using scanning electron microscope (SEM) at four stages: Baseline, after lesion formation, immediately after remineralization, and after pH cycling. Data were statistically analyzed with Statistical Package for the Social Sciences (SPSS), version 20. Results The degree of demineralization was significantly elevated in control group; however, no significant difference was found between fluoride varnish group and nano-HAP paste group (p < 0.001). Conclusion Nano-HAP paste showed promising long-term protective effect in terms of surface depositions and maintaining a smooth surface compared with fluoride varnish. Clinical significance Based on the findings of this study, nano-HAP paste might be recommended as alternative remineralizing agent with lower fluoride concentration than fluoride varnish that could be beneficial for children, pregnant females, and those who are at high risk of dental fluorosis. How to cite this article Daas I, Badr S, Osman E. Comparison between Fluoride and Nano-hydroxyapatite in Remineralizing Initial Enamel Lesion: An in vitro Study. J Contemp Dent Pract 2018;19(3):306-312.

scholarly journals In vitro comparison of the cariostatic effect between topical application of fluoride gels and fluoride toothpaste

2004 ◽  
Vol 12 (2) ◽  
pp. 121-126 ◽  
Author(s):  
Alberto Carlos Botazzo Delbem ◽  
Fernanda Lourenção Brighenti ◽  
Ana Elisa de Mello Vieira ◽  
Jaime Aparecido Cury
Keyword(s):  
Fluoride Concentration ◽  
Preventive Measure ◽  
Progression Rate ◽  
Surface Microhardness ◽  
Human Teeth ◽  
Cross Sectional ◽  
Fluoride Toothpaste ◽  
Fluoride Uptake ◽  
Ph Cycling

The aim of this study was to compare the effect of topical fluoride products [acidulated phosphate fluoride (APF) or neutral gel (NF) x fluoride toothpaste (MFP)], in respect to fluoride uptake and anticariogenic action. One hundred and twenty five blocks of human teeth, sorted in 5 groups according to the treatment, were submitted to pH cycling for ten days. The parameters analyzed were: fluoride uptake before and after pH cycling and surface (SMH) and cross-sectional (CSMH) microhardness of the enamel blocks. The results of fluoride concentration in enamel after the pH cycling showed an enhancement of fluoride uptake for all groups compared to sound control. No significant differences between APF and MFP were observed for surface microhardness, percentage change of surface microhardness and mineral loss. The volume percent mineral obtained from cross-sectional microhardness demonstrated that APF has a different lesion progression rate regarding subsurface carious lesion. The results suggest that professionally applied fluoride gel or frequent fluoride application in low concentration is a positive preventive measure for the control of dental caries.

scholarly journals Resistance against Erosive Challenge of Dental Enamel Treated with 1,450-PPM Fluoride Toothpastes Containing Different Biomimetic Compounds

2021 ◽  
Author(s):  
Nayanna L. S. Fernandes ◽  
L. da Cunha Juliellen ◽  
F. B. de Oliveira Andressa ◽  
H. P. Paulo D’Alpino ◽  
C. Fábio Sampaio
Keyword(s):  
Dental Enamel ◽  
In Vitro Study ◽  
The Other ◽  
Mineral Layer ◽  
Cross Sectional ◽  
X Ray ◽  
Weight Percentage ◽  
Elemental Analyses ◽  
Ph Cycling

Abstract Objectives This in vitro study aimed to characterize the superficial and subsurface morphology of dental enamel treated with fluoridated gels containing different biomimetic compounds after erosive challenge. Materials and Methods Bovine incisor teeth were sectioned to obtain enamel blocks (4 mm × 4 mm × 6 mm; n = 5) that were demineralized to create an artificial caries lesion and treated by pH cycling interspersed with exposure to fluoridated toothpaste slurries under agitation. During pH cycling (demineralization and remineralization for 2 and 22 hours, respectively) for 6 days, the enamel blocks were exposed to toothpaste slurries under agitation with one of the dental gels: Regenerate Enamel Science (NR-5 technology), Daily Regenerator Dental Clean (REFIX technology), and Sensodyne Repair & Protect (Novamin technology). The enamel blocks were subjected to an erosive challenge, immersed in 50% citric acid for 2 minutes, and then washed with plenty of distilled water. The surface and cross-sectional micromorphology were assessed using scanning electron microscope (SEM). The elemental analyses (weight percentage) were determined with an energy-dispersive X-ray spectroscopy (EDS). Results Enamel treated with the product containing REFIX technology presented a smoother surface morphology compared to the other treatments. The higher resistance to the erosive challenge can be attributed to a silicon-enriched mineral layer formed on the enamel induced by the REFIX-based toothpaste. This was not observed in the specimens treated with the other technology-containing toothpastes. Conclusion The REFIX technology seemed to be the most promising compared to the Novamin and NR-5 technologies. In addition to forming a surface mineralized layer, the enamel treated with REFIX technology associated with the pH cycling resisted a subsequent erosive challenge.

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