scholarly journals INVESTIGATION OF THE EFFECT OF ARTEMISIA L. HERB EXTRACTS ON THE PROGRESS OF THE TOXIC TETRACHLOROMETHANE LIVER DAMAGE

2020 ◽  
pp. 147-155
Author(s):  
R. A. Hrytsyk ◽  
O. A. Struk ◽  
V. M. Ivanochko
Keyword(s):  
Acute Toxicity ◽  
Liver Damage ◽  
Biochemical Parameters ◽  
Hepatoprotective Activity ◽  
Intragastric Administration ◽  
Artemisia Vulgaris ◽  
Artemisia Absinthium ◽  
Reference Drug ◽  
Toxic Liver Damage ◽  
Acute Toxic

Introduction. Artemisia L. genus species are the promising sources for the manufacturing of drugs with hepatoprotective activity. They are used as appetizing, anthelmintic, bactericidal, choleretic and anti-inflammatory re­medies. The pharmacological activity of Artemisia L. species is caused by the presence of different groups of biologically active substances. The aim of the study – to learn the acute toxicity and the effect of Artemisia absinthium and Artemisia vulgaris water-alcohol extracts on the progress of the toxic tetrachloromethane liver damage. Research Methods. The method of preclinical study of drugs safety was used to determine the acute toxicity. The investigation of hepatoprotective activity of Artemisia absinthium and Artemisia vulgaris extracts was performed using the model of acute tetrachloromethane hepatitis. Hepatoprotector of local manufacturer (“Silibor” tablets) was used as the reference drug. Results and Discussion. It was found that intragastric administration of Artemisia absinthium and Artemisia vulgaris extracts at the dose of 6000 mg/kg does not lead to the death of animals. There were no changes in the integral, hematological, biochemical parameters and in the morphological structure of the internal organs of experimental animals. It allows to characterize the extracts at this dose as almost non-toxic ones (V toxicity class, LD50>5000 mg/kg) according to the toxicity classification of substances. The results of Artemisia absinthium and Artemisia vulgaris extracts study indicate that they show the distinct hepatoprotective activity in condition of acute toxic liver damage. They suppress peroxide destructive processes and reduce the evolution of cytolysis syndrome and their effects are no inferior rather than the effect of tablets “Silibor”. Conclusions. The study of acute toxicity of Artemisia absinthium and Artemisia vulgaris extracts after their intragastric administration at the dose of 6000 mg/kg does not lead to the animals death. They were tidy and had the good appetite. The animals reacted adequately to sound and light stimulation. The processes of urination and defecation were unchanged. Breathing disorders and seizures were not observed. The investigated Artemisia ­absinthium and Artemisia vulgaris extracts demonstrate hepatoprotective activity in condition of acute toxic liver damage. This is proved by decreasing in the intensity of lipid peroxidation and in the toxicity of tetrachloromethane. Biochemical parameters of the animals’ blood and the liver homogenate became responded to the level of intact animals.

scholarly journals CHRONOPHARMACOLOGICAL FEATURES OF SILIMARIN’S HEPATOPROTECTOCAL ACTIVITY UNDER PARACETAMOL HEPATITIS IN RATS

2020 ◽  
pp. 63-71
Author(s):  
K. O. Kalko ◽  
S. M. Drogovoz ◽  
O. Ya. Mishchenko ◽  
L. B. Ivantsyk ◽  
K. O. Dehtiarova ◽  
...  
Keyword(s):  
Liver Damage ◽  
Hepatoprotective Activity ◽  
Time Of Day ◽  
The State ◽  
Acute Liver Damage ◽  
Toxic Liver Damage ◽  
Antioxidant Protection System ◽  
Toxicity Of Drugs ◽  
Acute Toxic

Determination of dependence of the efficiency and toxicity of drugs on the time of day or season is one of the most promising areas of modern experimental and clinical pharmacology. The aim of the study. Aim is to identify features of daily rhythms of silymarin influence on the state of antioxidant/prooxidant and cytolytic processes (the leading link in the implementation of hepatoprotective activity) with paracetamol in rats under conditions of acute toxic liver damage. Materials and methods. Chronopharmacological action features of silymarin were studied by use of the generic drug "Karsil" as an example (Sopharma, Bulgaria) with paracetamol in rats under conditions of acute toxic liver damage. The state of the processes of POL-AOS was evaluated by the content of TBA-active products (TBA-AP), the content of recovered glutathione, the activity of SOD, catalase in hepatocytes, and the cytolytic processes by the activity of ALT in serum. Results. There was established a certain chronoarchitectonics of the rhythms of the antioxidant protection system and the activity of the processes of peroxidation in rats under physiological conditions, which was not disturbed by paracetamol on the background of acute liver damage (with the exception of level of acrophase catalase activity). The same dynamics and commonality of rhythms occurred on the background of the use of silymarin. The maximum display of silymarin hepatoprotective activity was observed under conditions of its use in the morning (09.00) and in the evening (21.00). Conclusions. The use of silymarin during periods of maximal display of its hepatoprotective activity, in the morning (09.00) and in the evening (21.00) may be the way to increase its bioavailability. Chronopharmacological features of silymarin activity have been established and should be taken into account when to conduct its further chronopharmacological studies.

scholarly journals The chemokine receptor CXCR3 limits injury after acute toxic liver damage

2012 ◽  
Vol 92 (5) ◽  
pp. 724-734 ◽  
Author(s):  
Mirko Moreno Zaldivar ◽  
Marie-Luise Berres ◽  
Hacer Sahin ◽  
Andreas Nellen ◽  
Daniel Heinrichs ◽  
...  
Keyword(s):  
Liver Damage ◽  
Chemokine Receptor ◽  
Toxic Liver Damage ◽  
Acute Toxic

scholarly journals Comparative Evaluation of Hepatoprotective Activities of Trigonella foenum-graecum (seeds), Cymbopogon proximus and Acacia nilotica on a model of acute carbon tetrachloride hepatitis in rats

2021 ◽  
pp. 172-177
Author(s):  
Abbas Abdelhamid Mutawakel ◽  
V. I. Inchina ◽  
T. V. Tarasova ◽  
G. G. Varvanina ◽  
I. E. Trubitsyna
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Blood Cells ◽  
Intraperitoneal Administration ◽  
Inflammatory Infiltration ◽  
Herbal Preparations ◽  
Water Extracts ◽  
Toxic Liver Damage ◽  
Different Doses ◽  
Acute Toxic

An experimental study of the beneficial effect of herbal preparations on acute toxic liver damage with carbon tetrachloride (CCl4) in animals was conducted The goal is to Compare the hepatoprotective effect of three water extracts of herbal preparations: (I) Trigonella foenum-graecum (seeds), (II) Cymbopogon proximus, (III) Asasia nilotica Material and methods. To simulate acute toxic liver damage, 50% CCl4 was administered once intraperitonally in doses of 0.6 ml, 0.3 ml, and 0.2 ml. Control groups: K1-1 group intact animals without injections; K2: groups 2, 3, 4. introduced saline solution (FR) without Ssl4 in the volume of 2 ml. Experimental groups: 5, 6, 7 and 8 studied the hepatoprotective properties of aqueous solutions of the studied drugs after intraperitoneal administration of Ssl4 or FR. Researched herbs: I, II, III, in different doses were administered 2 ml of an aqueous solution in breeding 1:10, observation period 5 days, the blood, for research stabilized solution of sodium citrate in the ratio of 1:10, studied the properties of blood cells hematological analyzer PCE-90Vet (USA). The content of total protein, albumin, total cholesterol, triglycerides, urea, creatinine and transaminase activity - ALT, AST-were recorded in the blood serum using an automatic analyzer of the company “ HUMASTAR 600 “(Germany)», Results and discussion. Water extracts of preparations I and II prevented the reduction of red blood cells and platelets, and activated the macrophage pool. All the herb extracts studied prevent platelet reduction, however, but do not bring them up to the indicators of intact animals. The use of solution III prevented the growth of the cytolysis enzyme-ALT. All studied extracts in acute carbon tetrachloride intoxication. they warned of an increase in hypertriglyceremia. The introduction of water extracts II, III in the 1:10 ratio prevented the development of necrotic changes in hepatocytes, inflammatory infiltration, manifestations of fatty dystrophy, and preserved the architectonics of the liver lobule.

Hepatoprotector activity of iridoid glycosides with respect to heliotrine-induced acute toxic liver damage in mice

1999 ◽  
Vol 33 (8) ◽  
pp. 410-412 ◽  
Author(s):  
V. N. Syrov ◽  
A. N. Nabiev ◽  
Z. A. Khushbaktova ◽  
U. V. Zakhidov ◽  
M. S. Maksudov ◽  
...  
Keyword(s):  
Liver Damage ◽  
Iridoid Glycosides ◽  
Hepatoprotector Activity ◽  
Toxic Liver Damage ◽  
Acute Toxic

scholarly journals Hepatoprotective activity of Chamaecrista nigricans in Experimental Rats

2021 ◽  
pp. 20-27
Author(s):  
Ekambaram Gayathiri ◽  
Madepalli Byrappa Viswanathan
Keyword(s):  
Acute Toxicity ◽  
Drug Development ◽  
Liver Damage ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Histopathological Examination ◽  
Scientific Evidence ◽  
Hepatoprotective Activity ◽  
Serum Marker ◽  
Hepatoprotective Drug

An important ethnomedicinal plant Chamaecrista nigricans (Vahl) Greene, widely used for antipyretic, appetite, family planning, fevers, sore throat, wounds and various gastrointestinal disorders including diarrhoea and peptic ulcer. In the present study, acute toxicity and hepatoprotective activity of methanol extract alone were carried out in experimental animals approved by the Institutional Animal Ethics Committee. In acute toxicity studies, there was no mortality in animals when the extracts tested as per OECD guidelines. Regarding hepatoprotective activity, methanol leaf extract significantly reduced the increased level of serum marker enzymes such as ACP, ALP, ALT, AST and Total bilirubin. The protective effect of the methanol extract was also confirmed by histopathological examination which supports that the methanol leaf extract repaired the liver damage caused by CCl4. Thus the present study provides scientific evidence to the extracts of their hepatoprotective potential against liver damage and offers lead for further research in drug development. Keywords: Ethnomedicine; Chamaecrista Nigricans; Acute Toxicity; Hepatoprotective; Drug Development

scholarly journals Proteins of allogeneic hepatocytes and pharmacological preparations for the correction of immunometabolic disorders in experimental liver pathology

2021 ◽  
Vol 7 (2) ◽  
pp. 83-99
Author(s):  
Ekaterina S. Litvinova ◽  
Nikolay N. Konoplya ◽  
Anastasiya A. Shulginova ◽  
Anastasiya V. Kharchenko
Keyword(s):  
Liver Damage ◽  
Alcohol Intoxication ◽  
Culture Fluid ◽  
Rat Hepatocytes ◽  
Ethanol Solution ◽  
Intragastric Administration ◽  
Newborn Mice ◽  
Male Wistar Rats ◽  
Toxic Liver Damage ◽  
Peroxidation Processes

Introduction: The relationship of numerous metabolic shifts, disorders of hepatocytes functional activity resulting from hypoxia and toxic liver damage with the function of the immune system has not been sufficiently studied so far, nor have the most effective methods of pharmacological correction been established. Materials and Methods: The studies were carried out on 603 mature male Wistar rats and 45 mice. Acute toxic liver damage (ATLD) was modeled by intramuscular introduction of carbon tetrachloride; acute liver ischemia (ALI) was caused by clamping the hepatoduodenal ligament for 20 minutes; chronic alcohol intoxication (CAI) was modeled by forced intragastric administration of 20% ethanol solution for 60 days. Isolation of xenogeneic (murine) and allogeneic (rat) hepatocytes from newborn mice and rats was carried out according to the method of Berry and Friend (1969); culture fluid of hepatocytes and its protein fractions were prepared according to our developed techniques. The obtained biological material was intraperitoneally introduced into the rats with ATLD, ALI, and CAI. Results and Discussion: In all the models of the liver damage, there developed morphological and biochemical signs of the liver damage, impaired congenital and adaptive immunity, oxidative stress, increased lipid peroxidation processes. Conclusion: The introduction of allogeneic hepatocytes, culture fluid obtained on their basis,and proteins isolated from it with MW less than 130 kDa to the recipients with toxic and ischemic liver damage more effectively corrects the pathologic changes in the liver in comparison with xenogeneic hepatocytes, their culture fluid and pharmacological preparations (combinations of Essentiale N and Hypoxenum or Heptral and Mexicor).

scholarly journals Тhe Probiotics Genesis Effect on the Productive Health of Laying Hens

2020 ◽  
Vol 9 (4) ◽  
pp. 2793-2797
Keyword(s):  
Acute Toxicity ◽  
Blood Serum ◽  
Clinical Examination ◽  
Biochemical Parameters ◽  
Laying Hens ◽  
Food Additive ◽  
Delayed Type Hypersensitivity ◽  
Intragastric Administration ◽  
White Rats ◽  
Calcium Phosphorus

The article presents research results of the food additive Genesis effect on laying hens health. Experiments were conducted in 2 stages. During the first stage of experiments the acute toxicity of the food additive Genesis was studied on white rats of the Standard variety. Criteria for assessing the acute toxicity of the food additive Genesis were indicators of animals’ clinical and hematological status. Observation of the experimental rats for 14 days made it possible to establish that after intragastric administration of an aqueous solution of the food additive Genesis there are no toxic effects in the form of immediate and delayed-type hypersensitivity. This is confirmed by the results of the clinical examination of laboratory animals and the morphological study of blood. During the second stage of experiments the effect of Genesis on the organism of laying hens was studied. The condition of laying hens was assessed by the results of a clinical examination, an assessment of the biochemical composition of blood serum, as well as change in their productive qualities. The biochemical parameters of the blood serum of laying hens were identified: inorganic calcium, phosphorus, calcium-phosphorus ratio, alkaline phosphatase, urea, creatinine, creatine Kinase, cholesterol, total protein, glucose. It was established that the food additive Genesis has a positive effect on the clinical, biochemical parameters of the blood serum of laying hens as well as on the morphological parameters of chicken eggs. In addition, when using the Genesis, the efficiency of feed utilization increases.

Safety Evaluation of Eucalyptus globulus Essential Oils through Acute and Sub-acute Toxicity and Skin Irritation in Mice and Rats

2020 ◽  
Vol 14 (3) ◽  
pp. 187-195
Author(s):  
Berhan Mengiste ◽  
Tizazu Zenebe ◽  
Kassahun Dires ◽  
Ermias Lulekal ◽  
Awol Mekonnen ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Essential Oil ◽  
Eucalyptus Globulus ◽  
Biochemical Parameters ◽  
Skin Irritation ◽  
Toxicity Study ◽  
Subacute Toxicity ◽  
Acute Toxicity Study ◽  
Limit Test ◽  
Ointment Formulation

Background: The Eucalyptus globulus extractions have been used by the traditional healers to treat diseases in the study area. Our previous study revealed that the essential oil has antimicrobial and antifungal activity. This study determined phytochemical analysis, skin irritation, acute and subacute toxicity of Eucalyptus globulus essential oil in mice and rats. Methods: The phytochemicals were analyzed using GC-MS mass spectrometry. The acute toxicity study was determined at three dose levels of 1500 mg/kg, 1750mg/kg, and 2000 mg/kg. The essential oil limit test at a dose of 1000 mg/kg was administered to mice for 28 consecutive days for sub-acute toxicity study. The mice mortality, behavioral change, injury and other signs of illness were recorded once daily. Biochemical parameters were evaluated. Liver and kidney were analyzed for histopathological analyses. The 5% ointment formulation was applied to the rat skin to determine skin irritation effects. Results: The Eucalyptus globulus essential oil showed no effect on the mice at a dose of 1500mg/kg and below, but caused signs of toxicity and death at a dose of 1750mg/kg and above compared to the controls (p<0.05). The LD50 value was 1650 mg/kg. There was no significant difference (p > 0.05) in the body weights, gross abnormalities of the organs and biochemical parameters compared to the control at 1000 mg/kg subacute toxicity study. No histopathological changes were detected in the organs tested. The 5% ointment formulation did not show any abnormal skin reaction. Discussion: In the present study, the Eucalyptus globulus essential oil was comparable with other studies in terms of both chemical composition and its effects on sub-acute and topical application. Conclusion: This toxicity study demonstrated that Eucalyptus globulus essential oil is nontoxic at a relatively lower concentration.

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